Title:
USE OF RHOA IN CANCER DIAGNOSIS AND INHIBITOR SCREENING
Document Type and Number:
WIPO Patent Application WO/2015/033565
Kind Code:
A1
Abstract:
Disclosed are a novel cancer detection method, a screening method for an inhibitor and anti-cancer drug targeting a molecule related to cancer, and a cancer treatment agent, and specifically, a mutated RHOA polypeptide, a polynucleotide for coding said polypeptide, and a method for detecting cancer by using the polypeptide and polynucleotide. In addition, the following are also disclosed: a vector and host cell containing said polynucleotide; a screening method for a cancer treatment agent containing said polypeptide and/or said polynucleotide; and a cancer treatment agent containing an siRNA having an RHOA mutant silencing effect.
Inventors:
ABURATANI HIROYUKI (JP)
ISHIKAWA SHUMPEI (JP)
KAKIUCHI MIWAKO (JP)
NISHIZAWA TAKASHI (JP)
ISHIKAWA SHUMPEI (JP)
KAKIUCHI MIWAKO (JP)
NISHIZAWA TAKASHI (JP)
Application Number:
PCT/JP2014/004539
Publication Date:
March 12, 2015
Filing Date:
September 04, 2014
Export Citation:
Assignee:
UNIV TOKYO (JP)
FORERUNNER PHARMA RES CO LTD (JP)
FORERUNNER PHARMA RES CO LTD (JP)
International Classes:
C12N15/09; A61K31/7088; A61K48/00; A61P35/00; C07K14/705; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
| WO2011163436A1 | 2011-12-29 | |||
| WO2003030836A2 | 2003-04-17 | |||
| WO2003023063A1 | 2003-03-20 |
Foreign References:
| JP2012511031A | 2012-05-17 |
Other References:
WAHEED, F. ET AL.: "Affinity precipitation of active Rho-GEFs using a GST-tagged mutant Rho protein (GST-RhoA(G17A)) from epithelial cell lysates.", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 61, no. E3932, 2012, pages 1 - 5, XP055328836
LONGENECKER, K. ET AL.: "Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution.", ACTA CRYSTALLOGRAPHICA SECTION D: BIOLOGICAL CRYSTALLOGRAPHY, vol. 59, 2003, pages 876 - 880, XP055328840
J. SURG. ONCOL., vol. 107, no. 3, 2013, pages 230 - 6
CA CANCER, J. CLIN., vol. 61, no. 2, 2011, pages 69 - 90
LANCET, vol. 376, no. 9742, 2010, pages 687 - 97
"Result of HER2 status in Japanese metastatic gastric cancer: Prospective cohort study (JFMC44-1101", GASTROINTESTINAL CANCERS SYMPOSIUM, 2013
J. CLIN. ONCOL., vol. 29, no. 30, 2011, pages 3968 - 76
LANCET ONCOL., vol. 14, no. 6, 2013, pages 490 - 9
NAT. REV. CANCER, vol. 2, no. 2, 2002, pages 133 - 42
BIOCHIM. BIOPHYS. ACTA, vol. 1796, no. 2, 2009, pages 91 - 8
BIOCHIM. BIOPHYS. ACTA, vol. 1795, no. 2, 2009, pages 137 - 51
NAT. CELL BIOL., vol. 12, no. 5, 2010, pages 457 - 67
EMBOJ., vol. 14, 1995, pages 286
RIDLEY, INT. J. BIOCHEM. CELL. BIOL., vol. 29, no. 11, 1997, pages 1225 - 9, Retrieved from the Internet
VEGA ET AL., FEBS LETT., vol. 582, no. 14, 2008, pages 2093 - 101, Retrieved from the Internet
VEGA ET AL., FEBS LETT., vol. 582, no. 14, 2008, pages 2093 - 101
HARLOW; LANE: "Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY PRESS
HAMMERLING ET AL.: "Monoclonal Antibodies and T-Cell Hybridomas", 1981, ELSEVIER
SHARP, GENES DEV., vol. 13, no. 2, 1999, pages 139
HAMMOND ET AL., NATURE, vol. 404, no. 6775, 2000, pages 293
ELBASHIR ET AL., NATURE, vol. 411, no. 6836, 2001, pages 494
MIYAGISHI ET AL., NAT. BIOTECHNOL., vol. 20, no. 5, 2002, pages 497
LEE ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 500
JACQUE ET AL., NATURE, vol. 418, 2002, pages 435
FRUSCOLONI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, 2003, pages 1639
DYKXHOORN ET AL., NAT. REV. MOL. CELL BIOL., vol. 4, 2003, pages 457
URNOV ET AL., NATURE, vol. 435, no. 7042, 2005, pages 646
LOMBARDO ET AL., NAT. BIOTECHNOL., vol. 25, no. 11, 2007, pages 1298
DO ET AL., MUTAT. RES., vol. 740, no. 1-2, 2012, pages 34
SONG ET AL., NATURE MED., vol. 9, 2003, pages 347
See also references of EP 3042955A4
LONGENECKER, K. ET AL.: "Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution.", ACTA CRYSTALLOGRAPHICA SECTION D: BIOLOGICAL CRYSTALLOGRAPHY, vol. 59, 2003, pages 876 - 880, XP055328840
J. SURG. ONCOL., vol. 107, no. 3, 2013, pages 230 - 6
CA CANCER, J. CLIN., vol. 61, no. 2, 2011, pages 69 - 90
LANCET, vol. 376, no. 9742, 2010, pages 687 - 97
"Result of HER2 status in Japanese metastatic gastric cancer: Prospective cohort study (JFMC44-1101", GASTROINTESTINAL CANCERS SYMPOSIUM, 2013
J. CLIN. ONCOL., vol. 29, no. 30, 2011, pages 3968 - 76
LANCET ONCOL., vol. 14, no. 6, 2013, pages 490 - 9
NAT. REV. CANCER, vol. 2, no. 2, 2002, pages 133 - 42
BIOCHIM. BIOPHYS. ACTA, vol. 1796, no. 2, 2009, pages 91 - 8
BIOCHIM. BIOPHYS. ACTA, vol. 1795, no. 2, 2009, pages 137 - 51
NAT. CELL BIOL., vol. 12, no. 5, 2010, pages 457 - 67
EMBOJ., vol. 14, 1995, pages 286
RIDLEY, INT. J. BIOCHEM. CELL. BIOL., vol. 29, no. 11, 1997, pages 1225 - 9, Retrieved from the Internet
VEGA ET AL., FEBS LETT., vol. 582, no. 14, 2008, pages 2093 - 101, Retrieved from the Internet
VEGA ET AL., FEBS LETT., vol. 582, no. 14, 2008, pages 2093 - 101
HARLOW; LANE: "Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY PRESS
HAMMERLING ET AL.: "Monoclonal Antibodies and T-Cell Hybridomas", 1981, ELSEVIER
SHARP, GENES DEV., vol. 13, no. 2, 1999, pages 139
HAMMOND ET AL., NATURE, vol. 404, no. 6775, 2000, pages 293
ELBASHIR ET AL., NATURE, vol. 411, no. 6836, 2001, pages 494
MIYAGISHI ET AL., NAT. BIOTECHNOL., vol. 20, no. 5, 2002, pages 497
LEE ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 500
JACQUE ET AL., NATURE, vol. 418, 2002, pages 435
FRUSCOLONI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, 2003, pages 1639
DYKXHOORN ET AL., NAT. REV. MOL. CELL BIOL., vol. 4, 2003, pages 457
URNOV ET AL., NATURE, vol. 435, no. 7042, 2005, pages 646
LOMBARDO ET AL., NAT. BIOTECHNOL., vol. 25, no. 11, 2007, pages 1298
DO ET AL., MUTAT. RES., vol. 740, no. 1-2, 2012, pages 34
SONG ET AL., NATURE MED., vol. 9, 2003, pages 347
See also references of EP 3042955A4
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
Seiji Ono (JP)
Seiji Ono (JP)
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