Title:
VACCINATION OF SEX REVERSED HYBRID TILAPIA (OREOCHROMIS NILOTICUS X O. AUREUS) WITH AN INACTIVATED VIBRIO VULNIFICUS VACCINE
Document Type and Number:
WIPO Patent Application WO/2012/129375
Kind Code:
A3
Abstract:
Vibrio vulnificus can cause infections in economically important aquaculture-raised fish and is considered an opportunistic human-pathogen. V. vulnificus was isolated using biochemical and molecular methods. V. vulnificus isolate recovered from diseased tilapia was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. The isolate can cause disease in tilapia held in static salt water (1.5g sea salt/L). Fish vaccinated with formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer (vaccinated titers ranged from 32-64 and non- vaccinated titers ranged from 4-8). Vaccinated tilapia exhibited relative survival of 73 and 60% following challenge with the homologous isolate. Vaccinated tilapia exhibited relative survival values of up to 87.5% following challenge with a heterologous isolate. Use of a mineral oil adjuvant enhanced protection. This vaccine provides an effective means of preventing infections caused by biochemically and genetically diverse isolates of V. vulnificus in aquaculture.
Inventors:
SHOEMAKER CRAIG A (US)
LAFRENTZ BENJAMIN RYAN (US)
LAFRENTZ BENJAMIN RYAN (US)
Application Number:
PCT/US2012/030073
Publication Date:
December 27, 2012
Filing Date:
March 22, 2012
Export Citation:
Assignee:
US AGRICULTURE (US)
SHOEMAKER CRAIG A (US)
LAFRENTZ BENJAMIN RYAN (US)
SHOEMAKER CRAIG A (US)
LAFRENTZ BENJAMIN RYAN (US)
International Classes:
C12N1/20; A61K35/76; A61K39/106; A61K39/00
Foreign References:
| US7794730B2 | 2010-09-14 | |||
| US20100168203A1 | 2010-07-01 |
Other References:
EVA SANJUAN ET AL.: "Evaluation of genotypic and phenotypic methods to dis- tinguish clinical from environmental Vibrio vulnificus strains", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 75, no. 6, March 2009 (2009-03-01), pages 1604 - 1613
PATRICK C. THIAVILLE ET AL.: "Genotype is correlated with but does not pre- dict virulence of Vibrio vulnificus biotype 1 in subcutaneously inoculated, iron dextran-treated mice", INFECTION AND IMMUNITY, PUBLISHED AHEAD OF PRINT ON, vol. 79, no. 3, 3 January 2011 (2011-01-03), pages 1194 - 1207
FRANCISCO J. ROIG ET AL.: "pilF polymorphism-based PCR to distinguish Vibrio vulnificus strains potentially dangerous to public health", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 76, no. 5, March 2010 (2010-03-01), pages 1328 - 1333
CRAIG A. SHOEMAKER ET AL.: "Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus x 0. aureus) with an inactivated Vibrio vulnificus vaccine", BIOLOGICALS., vol. 39, no. 6, 7 October 2011 (2011-10-07), pages 424 - 429
PATRICK C. THIAVILLE ET AL.: "Genotype is correlated with but does not pre- dict virulence of Vibrio vulnificus biotype 1 in subcutaneously inoculated, iron dextran-treated mice", INFECTION AND IMMUNITY, PUBLISHED AHEAD OF PRINT ON, vol. 79, no. 3, 3 January 2011 (2011-01-03), pages 1194 - 1207
FRANCISCO J. ROIG ET AL.: "pilF polymorphism-based PCR to distinguish Vibrio vulnificus strains potentially dangerous to public health", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 76, no. 5, March 2010 (2010-03-01), pages 1328 - 1333
CRAIG A. SHOEMAKER ET AL.: "Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus x 0. aureus) with an inactivated Vibrio vulnificus vaccine", BIOLOGICALS., vol. 39, no. 6, 7 October 2011 (2011-10-07), pages 424 - 429
Attorney, Agent or Firm:
GOLDBERG, Joshua B. (112 S. West StreetAlexandria, Virginia, US)
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