VACCINE AGAINST AFRICAN SWINE FEVER VIRUS INFECTION

FIELD OF THE INVENTION

The present invention relates to attenuated African Swine Fever viruses. The attenuated viruses protect pigs against subsequent challenge with virulent virus. The present invention also relates to the use of such attenuated viruses to treat and/or prevent African Swine Fever.

BACKGROUND TO THE INVENTION

African Swine Fever (ASF)

African swine fever is a devastating haemorrhagic disease of domestic pigs caused by a double-stranded DNA virus, African swine fever virus (ASFV). ASFV is the only member of the Asfarviridae family and replicates predominantly in the cytoplasm of cells. Virulent strains of ASFV can kill domestic pigs within about 5-14 days of infection with a mortality rate approaching 100%.

ASFV can infect and replicate in warthogs (Phacochoerus sp.), bushpigs (Potamocherus sp.) and soft ticks of the Ornithodoros species (which are thought to be a vector), but in these species few if any clinical signs are observed and long term persistent infections can be established. ASFV was first described after European settlers brought pigs into areas endemic with ASFV and, as such, is an example of an “emerging infection”. The disease is currently endemic in many sub-Saharan countries and in Europe in Sardinia. Following its introduction to Georgia in the Trans Caucasus region in 2007, ASFV has spread extensively through neighbouring countries including the Russian Federation. In 2012 the first outbreak was reported in Ukraine and in 2013 the first outbreaks in Belarus. In 2014 further outbreaks were reported in pigs in Ukraine and detection in wild boar in Lithuania and Poland.

There is currently no treatment for ASF. Prevention in countries outside Africa has been attempted on a national basis by restrictions on incoming pigs and pork products, compulsory boiling of waste animal products under licence before feeding to pigs and the application of a slaughter policy when the disease is diagnosed. Prevention in Africa is based on measures to keep warthogs and materials contaminated by warthogs away from the herd.

There is thus a need for improved measures to control ASFV infection and prevent spread of the disease. African Swine Fever Virus (ASFV)

The complete genome sequences of ASFV isolate Benin 97/1 (a highly pathogenic virus from West Africa, Groupl), isolate OURT88/3 (non-pathogenic, attenuated virus from Portugal, Group 1) and isolate BA71V (Vero cell tissue culture adapted non-pathogenic virus, Group 1) have been compared (Chapman et al. 2008 J. Gen. Virol. 89: 397-408).

In the OURT88/3 genome, the multigene family (MGF) 360 18R (DP148R) gene, EP153R gene and EP402R gene are each interrupted by frameshift mutations. Additionally, the following MGF genes are absent from the OURT88/3 genome: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L, MGF 300 3L, MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R, and MGF 505 1R, 2R and 6R. The MGF 5053R gene is also truncated.

The sequences of the high-virulence Lisboa60 strain and the low-virulence NH/P68 strain have also been compared (Portugal et al. 2015 J. Gen. Virol. 96: 408-419).

In the NH/P68 genome, the MGF 360 18R (DP148R) gene, EP153R gene and EP402R gene are each interrupted by a premature stop codon. Additionally, the following MGF genes are absent from the NH/P68 genome: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L, MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R, and MGF 505 1R, 2R and 6R. The MGF 360 9L and MGF 5053R genes are also truncated.

Deletion of the DP148R gene from the virulent Benin 97/1 isolate reduced virulence and induced protection against challenge with the parental virus (Reis et al. 2017 J. Virol. 91, 24: e01428- 17).

SUMMARY OF THE INVENTION

Generally, the invention relates to an attenuated African Swine Fever virus in which expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted, whilst expression and/or activity of particular MGF genes is not disrupted.

The invention provides an attenuated African Swine Fever (ASF) virus in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 1103L, 6L, 7L, 8L, 10L, 11L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1 R, 2R and 6R. In a further aspect the invention provides an attenuated African Swine Fever (ASF) virus in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 110 11 L and 12L,

MGF 3606L, 10L, 11L, 12L, 13L, and 14L, and MGF 505 1R and 2R.

In a further aspect the invention provides an attenuated African Swine Fever (ASF) virus in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 1105L, 6L, 8L and 12L,

MGF 3606L, 10L, 11L, 12L, 13L, 14L and 21R, and MGF 505 1R and 2R.

The invention also provides a vaccine comprising an attenuated ASF virus of the invention.

The invention further provides a vaccine of the invention for use in treating and/or preventing ASF in a subject.

The invention further provides a method for treating and/or preventing ASF in a subject which comprises the step of administering to the subject an effective amount of a vaccine according to the invention.

The invention yet further provides a method of attenuating an ASF virus which comprises the step of disrupting the expression and/or activity of the following genes: DP148R, EP153R and EP402R.

DESCRIPTION OF THE FIGURES

Figure 1 shows confocal microscopy images of non-permeabilised cells expressing wild- type (A) or mutant (B) CD2v stained with sera from pigs immunised with attenuated ASFV containing a wild-type CD2v gene to detect surface expression.

Figure 2 shows exemplary images from a HAD assay. Vero cells were infected with modified vaccinia virus Ankara expressing T7RNA polymerase and transfected with plasmids (pcDNA3) expressing wild-type or mutant CD2v full-length proteins with a C- terminal HA epitope tag. Pig red blood cells were added and cells observed for attachment of red blood cells to the surface. HAD of red blood cells is observed around three cells transfected with a plasmid expressing wild-type Benin CD2v (A). Partial HAD is observed around one cell expressing CD2v with the Y102 residue mutated to D (B). No HAD is observed for cells expressing CD2v with residue E99 mutated to R (C).

Figure 3 depicts an alignment of the amino acid sequence of CD2v ligand-binding domain from different ASFV isolates of varying genotypes. E99 and equivalent residues in other isolates are highlighted in yellow.

Figure 4 shows exemplary images from a HAD assay. Vero cells were infected with modified vaccinia virus Ankara expressing T7RNA polymerase and transfected with plasmids (pcDNA3) expressing wild-type or mutant CD2v full-length proteins from Benin or Georgia strains with a C-terminal HA epitope tag. Pig red blood cells were added and cells observed for attachment of red blood cells to the surface. HAD of red blood cells is observed around four cells transfected with a plasmid expressing wild-type Benin CD2v (A) and around four cells transfected with a plasmid expressing wild-type Georgia CD2v (B). No HAD is observed for cells expressing Georgia CD2v with residue Q96 mutated to R (C) or for untransfected Vero cells (D).

Figure 5 shows WSL cells expressing CD2vE99R infected with BeninΔCD2v virus (A) or OURT88/3 virus (B), which has frame-shift interruptions in the EP153R and EP402R/CD2v genes. HAD is observed upon infected with BeninΔCD2v but not with OURT88/3.

Figure 6 shows porcine marrow cells infected with ASFV Benin 97/1 wild-type (B left panel) or ASFV Benin 97/1 ADP148RΔEP153R-CD2vE99R (A, B right panel).

Figure 7 shows levels of luciferase expression from an NF-kB dependent promoter in HEK293T cells transfected with an empty pcDNA3 vector (negative control) or a plasmid expressing DP148R. Cells were stimulated with I L-1 β (A) or TNFα (B) to induce NF-kB activation. Alternatively, NF-kB activation was induced by overexpression of the the two main kinases of the NF-kB pathway, IKKα (C) or IKKβ (D).

Figure 8 shows immunofluorescence images of HeLa cells transfected with HA-tagged DP148R and stained for HA tag (red) and NF-kB p65 sub-unit (green). Nuclei are shown in blue. Cells were unstimulated (top row) or stimulated with TNFα (middle row) or I L-1 β (bottom row) to induce NF-kB activation and p65 translocation to the nucleus. Arrows point to cells expressing DP148R. In stimulated cells expressing DP148R, p65 doe snot translocate to the nucleus. Figure 9 depicts the experimental protocol used to immunise, boost and challenge pigs with BeninΔD P148RΔEP153 RΔCD2v (Group A) and BeninΔDP148RΔEP153R-CD2vE99R (Group B) viruses.

Figure 10 shows rectal temperatures of pigs in Group A (A) and B (B) and the control Group E (C) during immunisation and challenge.

Figure 11 shows clinical scores of pigs in Group A (A) and B (B) and the control Group E (C) during immunisation and challenge.

Figure 12 shows viremia of pigs in Group A (A) and B (B) during immunisation and challenge.

Figure 13 shows the antibody response of pigs in Group A (A) and B (B) during immunisation and challenge measured using a commercially available competitive ELISA based on the VP72 (B646L) major capsid protein.

Figure 14 shows the cell-mediated immune response in Group A (A) and B (B) during immunisation and challenge. Peripheral blood mononuclear cells were collected pre- immunization, boost and challenge from pigs in Groups A and B and stimulated with ASFV Benin virus. Numbers of IFN gamma producing cells were measured by Elispot assay.

Figure 15 shows the number of ASFV specific lesions found in pigs from Groups E, A and B at post-mortem.

Figure 16 directly compares virus genome in blood after immunisation with BeninΔDP148R (A), BeninΔDPI 48RΔCD2v (B), BeninΔDP148RΔEP153RΔCD2v (C) and BeninΔDP148RΔEP153R-CD2vE99R (D).

DETAILED DESCRIPTION

African Swine Fever Virus (ASFV)

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF). The genome structure of ASFV is known in the art, as detailed in Chapman et al. 2008 J. Gen. Virol. 89: 397-408. ASFV is a large, icosahedral, double-stranded DNA virus with a linear genome containing at least 150 genes. The number of genes differs slightly between different isolates of the virus. ASFV has similarities to the other large DNA viruses, e.g., poxvirus, iridovirus and mimivirus. In common with other viral haemorrhagic fevers, the main target cells for replication are those of monocyte, macrophage lineage. Based on sequence variation in the C-terminal region of the B646L gene encoding the major capsid protein p72, 22 ASFV genotypes (l-XXII) have been identified. All ASFV p72 genotypes have been circulating in eastern and southern Africa. Genotype I has been circulating in Europe, South America, the Caribbean and western Africa. Genotype VIII is confined to four East African countries.

Examples of strains from some of the genotypes are given below:

Genotype I : OURT88/3; Brazil/79; Lisbon/60; BA715; Pret; Benin 97/1; IC/1/96; IC/576; CAM/82; Madrid/62; Malta/78; ZAR85; Katange63; Togo; Dakar59; Ourt88/1; BEN/1/97; Dom_Rep; VAL/76; IC/2/96; Awoshie/99; NIG/1/99; NIG/1/98; ANG/70; BEL/85; SPEC120; Lisbon/57; ASFV-Warm; GHA/1/00; GAM/1/00; Ghana; HOL/86; NAM/1/80; NUR/90/1; CAM/4/85; ASFV-Teng; Tegani; ASFV-E75.

Genotype II: Georgia 2007/1; POL/2015/Podlaskie (Polish strain); Belgium/Etalle/wb/2018; ASFV/Kyiv/2016/131; China/2018/AnhuiXCGQ

Genotype III: BOT 1/99

Genotype IV: ASFV- War; RSA/1/99/W

Genotype VI: MOZ 94/1

Genotype VII: VICT/90/1; ASFV-Mku; RSA/1/98

Genotype VIII: NDA/1/90; KAL88/1; ZAM/2/84; JON89/13; KAV89/1; DEZda; AFSV-Mal; Malawi LI L 20/1

Genotype IX: UGA/1/95

Genotype X: BUR/1/84; BUR/2/84; BUR/90/1; UGA/3/95; TAN/Kwh12; Hindell; ASFV-Ken; Virulent Uganda 65.

ASFV isolates

The attenuated ASF virus of the present invention may be derivable from a wild-type ASF virus isolate, but includes mutations in its genome such that the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted.

The term “wild-type” indicates that the virus existed (at some point) in the field, and was isolated from a natural host, such as a domestic pig, tick or warthog. ASFV isolates described to date are summarised in Table 1 below, together with their Genbank Accession numbers. Table 1 The genome of the attenuated ASFV of the invention may correspond to any ASFV genotype. The genome of the attenuated ASFV of the invention may essentially correspond to any ASFV genotype.

The term “corresponds to” means that the remainder of the genome of the attenuated ASFV of the invention is the same as a wild-type strain (i.e. a virus that existed at some point in the field). “The remainder of the genome” refers to all genes other than the disrupted genes DP148R, EP153R and EP402R. In other words, the genes of the attenuated ASFV of the invention may be the same as the genes of the wild-type strain, except for the genes DP148R, EP153R and EP402R. In an embodiment the genes of the attenuated ASFV of the invention are the same as the genes of the wild-type strain, except for DP148R, EP153R and EP402R.

The disrupted genes DP148R, EP153R and EP402R may also correspond to the wild-type strain. In an embodiment the genes DP148R, EP153R and EP402R correspond to the wild- type strain. In such an embodiment (i.e. where DP148R, EP153R and EP402R correspond to the wild-type strain) expression and/or activity of DP148R, EP153R and EP402R may be disrupted by one or more mutation in an intergenic region such as a promoter. In other words, the DP148R, EP153R and EP402R genes are the same as in the wild-type genome but their expression or activity is altered by mutation of a non-genic sequence. Thus all of the genes of the attenuated ASFV of the invention may be the same as the genes of the wild-type strain. In an embodiment all genes of the attenuated ASFV of the invention are the same as the genes of the wild-type strain.

The term “essentially corresponds to” means the same as “corresponds to” with the additional exception that the remainder of the genome may comprise one or more mutations. The one or more mutations may be in other genes (i.e. not in the genes DP148R, EP153R and EP402R).

The genome of the attenuated ASFV may correspond or essentially correspond to genotype I. The genome of the attenuated ASFV may correspond or essentially correspond to genotype II. The genome of the attenuated ASFV may correspond or essentially correspond to genotype III. The genome of the attenuated ASFV may correspond or essentially correspond to genotype IV. The genome of the attenuated ASFV may correspond or essentially correspond to genotype V. The genome of the attenuated ASFV may correspond or essentially correspond to genotype VI. The genome of the attenuated ASFV may correspond or essentially correspond to genotype VII. The genome of the attenuated ASFV may correspond or essentially correspond to genotype VIII. The genome of the attenuated ASFV may correspond or essentially correspond to genotype IX. The genome of the attenuated ASFV may correspond or essentially correspond to genotype X.

The genome of the attenuated ASFV may correspond or essentially correspond to genotype I. The genome of the attenuated ASFV may correspond or essentially correspond to genotype II.

Preferably, the genome of the attenuated ASFV may correspond or essentially correspond to genotype I.

The genome of the attenuated ASFV of the invention may correspond or essentially correspond to that of a virulent ASFV strain. Known virulent ASF virus strains include: Georgia 2007/1, Benin 97/1, Kenyan, Malawi UI20/1, Pretorisuskop/96/4 and Tengani 62. The genome of the attenuated ASFV may correspond or essentially correspond to that of the Benin 97/1 strain. The genome of the attenuated ASFV may correspond or essentially correspond to that of the Georgia 2007/1 strain.

The genome of the attenuated ASFV may correspond or essentially correspond to that of the Benin 97/1 strain.

The genome of the attenuated ASFV of the invention may correspond or essentially correspond to that of an ASFV strain whose virulence is currently unknown, for example: Mkuzi, Warmbaths and Warthog.

In an embodiment the genome of the attenuated ASFV of the invention does not correspond to that of OURT88/3. In an embodiment the genome of the attenuated ASFV of the invention does not correspond to that of NH/P68. In an embodiment the attenuated ASFV of the invention is not OURT88/3. In an embodiment the attenuated ASFV of the invention is not NH/P68. In an embodiment the attenuated ASFV of the invention is neither OURT88/3 nor NH/P68.

The complete genome for the African swine fever virus Benin 97/1 pathogenic isolate is given in Genbank Locus: AM712239.1. The complete BA71 isolate genome encodes 151 open reading frames (ORFs), the Benin 97/1 isolate encodes 157 ORFs and the OURT88/3 isolate encodes 151 ORFs.

Multigene families (MGFs)

ASFV contains five multi-gene families which are present in the left and right variable regions of the genome. The MGFs are named after the average number of codons present in each gene: MGF100, 110, 300, 360 and 505/530. The N-terminal regions of members of MGFs 300, 360 and 505/530 share significant similarity with each other. It has been shown the MGF 360 and 505 families encode genes essential for host range function that involves promotion of infected-cell survival and suppression of type I interferon response.

The attenuated ASFV according to the present invention comprises a functional version of one or more of the following genes: multigene family (MGF) 1103L, 6L, 7L, 8L, 10L, 11 L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1 R, 2R and 6R.

In a further aspect the invention provides an attenuated African Swine Fever (ASF) virus in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 110 11 L and 12L,

MGF 3606L, 10L, 11 L, 12L, 13L, and 14L, and MGF 505 1R and 2R.

Suitably, in one embodiment the invention provides an ASFV in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 1105L, 6L, 8L, and 12L, and MGF 3606L, 10L, 11 L, 12L, 13L, 14L and 21R, and MGF 505 1R and 2R.

Suitably, in one embodiment the invention provides an ASFV in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 1106L, 8L, and 12L, and MGF 3606L, 10L, 11 L, 12L, 13L, 14L and 21R, and MGF 505 1R and 2R.

Suitably, in one embodiment the invention provides an ASFV in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 110 12L, and MGF 3606L, 10L, 11 L, 12L, 13L, 14L, and MGF 505 1R and 2R. Suitably, in one embodiment the invention provides an ASFV in which the expression and/or activity of the genes DP148R, EP153R and EP402R is disrupted; and which comprises a functional version of one or more of the following genes: multigene family (MGF) 3606L, 10L, 11L, 12L, 13L, and 14L, and MGF 505 1 R and 2R.

The location of some of these genes in the genomes of a variety of ASFV strains is provided below in Table 2. The sequence identity of each gene to the corresponding Benin 97/1 gene is also provided.

Table 2a - MGF 360-10L

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 20677 19607 100

Georgia 2007/1 26438 25368 93

BA71V Deleted

OURT88/3 Deleted

Kenya 1950 30628 29563 82

Malawi-Lil/20 24802 23763 91

Mkuzi 1979 28139 27069 99

Pretorisuskop/96/4 27186 26116 96

Tengani 62 21600 20530 92

Warmbaths 26361 25322 94

Warthog 23130 22091 95

E75 19981 18911 100

Table 2b - MGF 360-11L

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 21764 20703 100 Georgia 2007/1 27526 26465 97 BA71V Deleted OURT88/3 Deleted

Kenya 1950 31716 30655 84

Malawi-Lil/20 25892 24831 86

Mkuzi 1979 29225 28164 99

Pretorisuskop/96/4 28275 27214 97

Tengani 62 22688 21627 97

Warmbaths 27449 26388 97

Warthog 24218 23157 97

E75 21068 20007 100 Table 2c- MGF 360-12L

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 24668 23616 100 Georgia 2007/1 30434 29382 96 BA71V Deleted OURT88/3 Deleted

Kenya 1950 34566 33549 90

Malawi-Lil/20 28731 27682 92

Mkuzi 1979 32125 31073 97

Pretorisuskop/96/4 31150 30098 95

Tengani 62 25592 24540 96

Warmbaths 30346 29294 96

Warthog 27088 26036 96

E75 23971 22920 99

Table 2d- MG F 360-13L

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 25901 24840 100 Georgia 2007/1 31656 30595 97 BA71V Deleted OURT88/3 Deleted

Kenya 1950 35812 34757 90

Malawi-Lil/20 29980 28925 91

Mkuzi 1979 33347 32286 99

Pretorisuskop/96/4 32368 31307 95

Tengani 62 26814 25753 95

Warmbaths 31559 30498 95

Warthog 28338 27277 95

E75 25204 24143 99 Table 2e - MGF 360- 14L

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 27146 26073 100 Georgia 2007/1 32913 31840 99 BA71V Deleted OURT88/3 Deleted

Kenya 1950 37194 36121 92

Malawi-Lil/20 31266 30193 92

Mkuzi 1979 34620 33547 99

Pretorisuskop/96/4 33598 32525 97

Tengani 62 28056 26983 97

Warmbaths 32820 31747 96 Warthog 29568 28495 97

E75 26449 25376 100

Table 2f- MGF 505-1 R

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 21971 23566 100 Georgia 2007/1 27734 29329 95 BA71V Deleted OURT88/3 Deleted

Kenya 1950 31904 33496 91

Malawi-Lil/20 26041 27627 93

Mkuzi 1979 29425 31020 96

Pretorisuskop/96/4 28449 30044 94

Tengani 62 22891 24486 95

Warmbaths 27651 29246 95

Warthog 24387 25982 95

E75 21275 22870 100

Table 2g - MGF 505-2R

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 27352 28932 100

Georgia 2007/1 33119 34699 99

BA71V 17725 19304 99

OURT88/3 29532 29981 76

Kenya 1950 37419 38985 90

Malawi-Lil/20 31541 33121 93

Mkuzi 1979 34826 36406 99

Pretorisuskop/96/4 33795 35374 97

Tengani 62 28261 29830 98

Warmbaths 33029 34597 96

Warthog 29773 31352 97

E75 26655 28236 99

Table 2h - MGF 505-3R

Strain Start Nucleotide Number Stop Nucleotide number % Nucleotide Identity

Benin 97/1 29019 29861 100 Georgia 2007/1 34786 35625 96 BA71V 20398 21915 100 OURT88/3 20850 22367 100 Kenya 1950 40295 41815 89 Malawi-Lil/20 34322 35842 89 Mkuzi 1979 37500 39017 99 Pretorisuskop/96/4 36480 37985 89

Tengani 62 30926 32443 90

Warmbaths 35712 37232 93

Warthog 32449 33954 90

E75 29330 30847 100

The gene (i.e. nucleotide) sequences of these genes from different strains are given below.

MGF 1105L gene sequences

SEQ ID No. 266 - China/2018/AnhuiXCGQ (genotype II) 9145-8528 MGF 110 5L/6L fusion

ATGTTGGTGATCTTCTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGAGCCTCCAGAGGAAGAACTCGAATACTGGTGTGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGACGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAGGGTATCTACCATACCATGAATTGCAACCAGGTCTTAGGGCTA CCTAATCAACCAGCTGGACAACT TCATCCAACGGATAATCCTCCACAAGAAGAACTTGAATACTGGTGCACTTATACAGAAAA CTGCAAGTTTTGCTGGAATTGTC AAAATGGCCTTTGTGAGGGCAAATTGGAGAATACAACAATTCTTGAAAATGAGTATGTGC AATCATGTATAGTTTCCCGCTGG CTAAATAAATGTATGTATGATCTAGGACAGGGGATTCACCATGTAATGGCCTGTTCTGAA CCAAAGCCCTGGAATCCTTACAA AAT CTTAAAGAGAGAGT GGAAAGAAAATAATAGTTAA

SEQ ID No. 267 - Georgia 2007/1 (genotype II) 9140-8523 MGF 110 5L/6L fusion

ATGTTGGTGATCTTCTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGAGCCTCCAGAGGAAGAACTCGAATACTGGTGTGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGACGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAGGGTATCTACCATACCATGAATTGCAACCAGGTCTTAGGGCTA CCTAATCAACCAGCTGGACAACT TCATCCAACGGATAATCCTCCACAAGAAGAACTTGAATACTGGTGCACTTATACAGAAAA CTGCAAGTTTTGCTGGAATTGTC AAAATGGCCTTTGTGAGGGCAAATTGGAGAATACAACAATTCTTGAAAATGAGTATGTGC AATCATGTATAGTTTCCCGCTGG CTAAATAAATGTATGTATGATCTAGGACAGGGGATTCACCATGTAATGGCCTGTTCTGAA CCAAAGCCCTGGAATCCTTACAA AAT CTTAAAGAGAGAGT GGAAAGAAAATAATAGTTAA

SEQ ID No. 268 - Ken05/Tk1 9698-9327 MGF 110 5L

ATGTTGGTGATCTTCTTGGGACTCCTTGGCCTGCTGGCCAACCAGGTCTTAGGGCTA CCTAATCAACCAGCTGGACAACTTCA TCCAACGGATAATCCTCCAAAAGAAGAACTTGGATACTGGTGCACTTACACGGAAAGCTG TAAATTTTGCTGGAACTGTCAAA ATGGCCTTTGTGAGGGCAAATTGGAGAATACAACAATTCTTGAAAATGAGTATGTGCAAT CATGTATAGTTTCCCGCTGGCTA AATAAATGTATGTATGATCTAGGACAGGGGATTGACCATGTAATGGCCTGTTCTGAACCA AAGCCCTGGAATCCTTACAAAAT C T T AAAGAGAGAG T G GAAAAAAAAT GT CAG C CAAAAT TAA

SEQ ID No. 269 - Ken06.Bus 6224-5853 MGF 1105L

ATGTTGGTGATCTTCTTGGGACTCCTTGGCCTGCTGGCCAACCAGGTCTTAGGGCTA CCTAATCAACCAGCTGGACAACTTCA TCCAACGGATAATCCTCCAAAAGAAGAACTTGGATACTGGTGCACTTACACGGAAAGCTG TAAATTTTGCTGGAACTGTCAAA ATGGCCTTTGTGAGGGCAAATTGGAGAATACAACAATTCTTGAAAATGAGTATGTGCAAT CATGTATAGTTTCCCGCTGGCTA AATAAATGTATGTATGATCTAGGACAGGGGATTCACCATGTAATGCCCTGTTCTGAACCA AAGCCCTGGAATCCTTA CAAAAT CTTAAAGAAAGAGTGGAAAGAAAATGTCAGCCAAAATTAA

SEQ ID No. 270 - Malawi Lil-20/1/1983 7878-7516 MGF 1105L

ATGTTGGTTATCTTCTTGGGAATTCTTGGCCTTCTGGCCAACCAGGTCTCAAGCCAG CTCGTTGGACAACTTCATCCAACGGA

AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTT TTGCTGGGACTGTCAAGATGGCCTTT

GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTA TAGTTTCCCGCTGGCTAAATAAATGT

ATGTATGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAACCAAAGTAC TGGAATCCTTATAAAATCTTAAAGAA

AGAGTGGAAAGAAAATAACAGCCAAAATTAA

SEQ ID No. 271 - Mkuzi 1979 (genotype XII) 10334-9972 MGF 1105L ATGTTAGTAATCTTCTTGGGAATTCTTGGCCTTCTGGCCAACCAGGTCTCAAGCCAGCTC GTTGGACAACTTCATCCAACGGA

AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTT TTGCTGGGACTGTCAAGATGGCCTTT

GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTA TAGTTTCCCGCTGGCTAAATAAATGC

ATGTATGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAACCAAAGTAC TGGAATCCTTATAAAATCTTAAAGAA

AGAGTGGAAAGAAAATAACAGTCAAAATTAA

SEQ ID No. 272 - Pretorisuskop/96/4 (genotype XX/I) 9424-9059 MGF 1105L

ATGTTAGTAATCTTCTTGGGAATTCTTGGCCTTCTGGCCAACCAGGTCTCAAGCCAG CTCGTTGGACAACTTCATCCAACGGA

AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTT TTGCTGGGACTGTCAAAATGGCCTTT

GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTA TAGTTTCCCGCTGGCTAAATAAATGT

ATGTATGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAGCCAAAATAC TGGAATCCTTATAAAATCTTAAAGAA

AGAGTGGAAAGAAAATAACAGCCAAAATAAATAA

SEQ ID No. 273 - Tengani62 (genotype V/l) 8803-8447 MGF 1105L

ATGTTAGTCATCTTCTTGGGAATTCTTGGCCTTCTGGTCAACCAGGTCTCAAGCCAG CCAGTTGGACAACTTCATCCAACGGA AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTTTTG CTGGGACTGTCAAAATGGCCTTT GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTATAG TTTCCCGCTGGCTAAATAAATGT ATGTATGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAACCAAAATACTGG AATCCTTATAAAATCTTAAAGAA AGAGT GGAAAGAAAATAATAGTTAA

SEQ ID No. 274 - Warmbaths (genotype 11 I/I) 8649-8284 MGF 1105L

ATGTTAGTAATCTTTTTGGGAATTCTTGGCCTTCTGGCCAACCAGGTCTCAAGCCAG CTCGTTGGACAACTTCATCCAACGGA AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTTTTG CTGGGACTGTCAAAATGGCCTTT GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTATAG TTTCCCGCTGGCTAAATAAATGT ATGTACGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAACCAAAATACTGG AATCCTTATAAAATCTTAAAGAA AGAGT GGAAAGAAAATAACAGCCAAAATAAATAA

SEQ ID No. 275 - Warthog (genotype IV) 7349-6984 MGF 1105L

ATGTTAGTAATCTTCTTGGGAATTCTTGGCCTTCTGGCCAACCAGGTCTCAAGCCAG CTCGTTGGACAACTTCATCCAACGGA AAATCCTTCAGAGAATGAACTTGAATACTGGTGCACTTACATGGAATGTTGCCAGTTTTG CTGGGACTGTCAAAATGGCCTTT GTGTGAATAAGTTGGGAAATACAACAATTCTTGAAAATGAGTATGTGCATCCATGTATAG TTTCCCGCTGGCTAAATAAATGT ATGTACGATCTGGGTCAAGGCATTGATCATGTAATGGTCTGTTCTCAACCAAAATACTGG AATCCTTATAAAATCTTAAAGAA AGAGT GGAAAGAAAATAACAGCCAAAATAAATAA

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 1105L. Suitably the functional version of MGF 110 5L comprises the sequence of SEQ ID No. 266, 267, 268, 269, 270, 271, 272, 273, 274 or 275. Suitably the functional version of MGF 110 5L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 266, 267, 268, 269, 270, 271 , 272, 273, 274 or 275. Suitably the functional version of MGF 110 5L consists of the sequence of SEQ ID No. 266, 267, 268, 269, 270, 271, 272, 273, 274 or 275.

MGF 1106L gene sequences

SEQ ID No. 35 - Ken05/Tk1 MGF 1106L

ATGTTGGTAATCTTTTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTCTCAAGTCAA CCAGATGGACAACTTCGTCCAACAGA GGATCCTCCAGAAGAAGAACTTAAATATTGGTGCACCTACATGGAAAGTTGCCAGTTTTG TTGGGACTGCCAAGATGGCAATT GTATAAACAAAGTAGATGGGTCAGTCATTTATAAAAATGAGTTTGTGCGACCATGTTCAG TTTCCCGCTGGATGAATAAATGT ATGTATGATTTAAATAAGGGTATCTATCATACAATGAATTGTTCTCAGCCACAGTCTTGG AATCCCTACAAATACTTCAGGAA G GAGT GGAAAAAAGAT GAACT CTAG SEQ ID No. 36 - Ken06.Bus MGF 1106L

ATGTTGGTGATCTTTTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTCTCAAGTCAG CCAGTTGGACAACTTCGTCCAACAGA GGACCCTCCAGAGAATGAACTCGAATATTGGTGCACCTACATGGAAAGTTGCCAGTTTTG CTGGGACTGTCAAGATGGCAATT GTATAAACAAAGTAGATGGGTCAGTCATTTATAAAAATGAGTATGTGCAACCATGTTCAG TTTCCCGCTGGCTAAATAAATGT ATGTATGATTTAGATAAGGGTATCTATCATACAATGAACTGTTCTCAGCCACGGTCTTGG AATCCCTACAAATGTTTCAGGAA G GAGT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 37 - Kenya 1950 MGF 1106L

ATGTTGGTGATCTTTTTGGGAATTCTTGGCCTTATGGCCAGCCAGGTCTTAGGGCTA CCATCTAATCAACCAACTGGACAACT TCGTCCAACAGAGGATCCTCCAGAGGAAGAACTCGAATACTGGTGTGCCTACATGGAAAG TTGCCAGTTTTGCTGGGACTGCC AAGATGGCAATTGCATAAACAAAATAGATGGGTCAGTCATTTATAAAAATGAGTTTGTGC GACCATGTTCAGTTTCCCGCTGG ATGGATAAATGTATGTATGATTTAAATAAGGGTATCTATCATACAATGAATTGTTCTCAG CCACAGTCTTGGAATCCCTACAA ATACT T CAG GAAG GAGT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 38 - Malawi Lil-20/1 (1983) MGF 1106L

ATGTTGGTGACCTTTTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTCTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGATCCTCCAGAGGAAGAACTCGAATACTGGTGCGCCTACATGGAAAGTTGCCAGTTTTG CTGGGACTGCCAAGATGGCAATT GTATAAACAAAATAGATGGGTCAGTCATTTATAAAAATGAGTATGTGCGACCATGTTCAG TTTCCCGTTCGATGGATAAATGT ATGTATGATTTAAATAAGGGTATCTACCATAGCATGAGCTGTTCTGACCCAAAGGCCTGG AATCCCTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 39 - Mkuzi 1979 MGF 1106L

ATGTTGGTGATCTTTTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAA CTCGTTGGACAACTTCGACCAACAGA GGATCCTCCAGAGGAAGAACTCGAATACTGGTGCGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGATGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAAGCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAAGGTATCTACCATACCATGAATTGTTCTCAGCCATGGTCTTGG AATCCTTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 40 - Pretorisuskop/96/4 MGF 110 6L

ATGTTGGTGATCTTCTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGATCCTCCAGAGGAAGAACTCGAATACTGGTGCGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGATGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAAGGTATCTACCATACCATGAATTGTTCTCAGCCATGGTCTTGG AATCCTTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 41 - Tengani62 MGF 1106L

ATGTTGGTGATCTTCTTGGGAATTTTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGATCCTCCAGAGGAAGAACTCGAATACTGGTGTGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGATGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAGGGTATCTACCATACCATGAATTGTTCTCAGCCATGGTCTTGG AATCCTTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 42 - Warmbaths MGF 1106L

ATGTTGGTGATCTTCTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGTCCAACAGA GGATCCTCCAGAGGAAGAACTCGAATACTGGTGCGCCTACATGGAAAGTTGCCAATTTTG CTGGGACTGCCAAGATGGCACTT GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTCTGG TTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAAGGTATCTACCATACCATGAATTGTTCTCAGCCATGGTCTTGG AATCCTTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

SEQ ID No. 43 - Warthog MGF 1106L

ATGTTGGTGATCTTCTTGGGAATTCTTGGCCTTCTGGCCAGCCAGGTTTCAAGTCAG CTCGTTGGACAACTTCGACCAACAGA

GGATCCTCCAGAGGAAGAACTCGAATACTGGTGCGCCTACATGGAAAGTTGCCAATT TTGCTGGGACTGCCAAGATGGCACTT

GTATAAACAAAATAGATGGGTCAGTAATTTACAAGAATGAGTATGTGAAATCATGTC TGGTTTCCCGTTGGCTGGATAAATGT ATGTATGATTTAGATAAAGGTATCTACCATACCATGAATTGTTCTCAGCCATGGTCTTGG AATCCTTACAAATACTTCAGGAA G GAAT GGAAAAAAGAT GAACT CTAG

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 1106L. Suitably the functional version of MGF 110 6L comprises the sequence of SEQ ID No. 35, 36, 37, 38, 39, 40, 41 , 42 or 43. Suitably the functional version of MGF 110 6L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 35, 36, 37, 38, 39, 40, 41, 42 or 43. Suitably the functional version of MGF 110 6L consists of the sequence of SEQ ID No. 35, 36, 37, 38, 39, 40, 41, 42 or 43.

MGF 1107L gene sequences

SEQ ID No. 247 - China/2018/AnhuiXCGQ MGF 110 7L

ATGCTGGTGATTATCCTGGGAATTATTGGCCTGCTGGCCAGTAGCAACCTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG

ACATCTTCCTCTAACATTTGAACCCCCAGAGAATGAACTTGGATACTGGTGCACTTA TGTGGAAAGCTGTCGGTTCTGCTGGG

ATTGTGAAGATGGGATTTGTACCAGCAGGGTTTGGGGAAACAACTCCACAAGTATTA TTGAGAATGACTATGTAAAATATTGT

GAGGTTTCCCGCTGGGGTGACCTATGTAGATATGATGTGGAGGAGCACATTTACCAT AGCATGAACTGTTCTGACCCAAAGCC

CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAACATCCCAGAAA GGATTTGAAAAAAGATGAATTCTAG

SEQ ID No. 248 - Georgia 2007/1 MGF 110 7L

ATGCTGGTGATTATCCTGGGAATTATTGGCCTGCTGGCCAGTAGCAACCTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG

ACATCTTCCTCTAACATTTGAACCCCCAGAGAATGAACTTGGATACTGGTGCACTTA TGTGGAAAGCTGTCGGTTCTGCTGGG

ATTGTGAAGATGGGATTTGTACCAGCAGGGTTTGGGGAAACAACTCCACAAGTATTA TTGAGAATGACTATGTAAAATATTGT

GAGGTTTCCCGCTGGGGTGACCTATGTAGATATGATGTGGAGGAGCACATTTACCAT AGCATGAACTGTTCTGACCCAAAGCC

CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAACATCCCAGAAA GGATTTGAAAAAAGATGAATTCTAG

SEQ ID No. 249 - Ken05/Tk1 MGF 110 7L

ATGCTGGTGATTATCCTGGGAGTTATTGGCCTGCTGGCCAGTAGCAACTTGGTTTCA TCCTCCACTTCTACTCGGATAGGTGG

ACATCTTCCTCTAACATTTGATCCTCCAGAGAATGAACTTGGATACTGGTGCACTTA TGTGGAAAGCTGTCGGTTCTGCTGGG

ATTGTGAAGATGGGGTCTGTACTAGCAGGATCTGGGGGAACAACTCCACAAGTATTG TTGAGAACAGCTATGTAAAATATTGT

GAAGTTTCCCGCTGGGGTGACCAGTGTAGATATGATGTGGAGGAGCGCATTTACCAT ACCATGAACTGTTCTGACCCAAAACC

CTGGAATCCTTATAAAATTACAAGGGTGGAGTGGAAAAAGAATGAACATTTCAGAAA GGATTTGAAAAAAGATGAATTCTAG

SEQ ID No. 250 - Kenya 1950 MGF 110 7L

ATGCTGGTGATTATCCTGGGAGTTATTGGCCTGCTGGCCAGTAGCAACTTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG

ACATCTTCCTCTAACATTTGATCCTCCAGAGAATGAACTTGGATACTGGTGCACTTA TGTGGAAAGCTGTCGGTTCTGCTGGG

ATTGTGAAGATGGGGTCTGTACTAGCAGGATCTGGGGGAACAACTCCACAAGTATTG TTGAGAACAGCTATATAAAATATTGT

GAAGTTTCCCGCTGGGGTGACCAGTGTAGATATGATGTGGAGGAGCACATTTACTAT ACCATGAACTGTTCTGACCCAAAGCC

CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAACATTTCAGAAA GGATTTGAAAAAAGATGAATTCTAG

SEQ ID No. 251 - Malawi Lil-20/1 (1983) MGF 110 7L

ATGCTGGTGATTATCCTGGGAGTTATTGGCCTGCTGGCCAGTAGCAACTTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG ACATCTTCCTCTAACATTTGATCCTCCAGAGAATGAACTTGGATACTGGTACACTTATGT GGAAAGTTGTCGGTTCTGCTGGG ATTGTGAAGATGGGGTCTGTACTAGCAGGGTTTGGGGAAACAACTCCACAAGTATTGTTG AGAATGACTATGTAAAATATTGT GAGGTTTCTCGCTGGGGTGACCAATGTAGATATGATGTGGAGGAGCACATTTACTATACC ATGAACTGTTCTGACCCAAAGCC C T G GAAT C C T T AT AAAAT T G C AAAG GAAG GAGT G G AAAAAG GAT GA

SEQ ID No. 252 - Mkuzi 1979 MGF 110 7L

ATGCTGGTGATTATCCTGGGAGTTATTGGCCTGCTGGCCAGTAGCAACTTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG

ACATCTTCCTCTAACAGAGGATCCTCCAGAGAATGAACTTGGATACTGGTGCACTTA TGTGGAAAGCTGTCGGTTCTGCTGGG

ATTGTGAAGATGGGATTTGTACTAGCAGGGTTTGGGGAAACAACTCCACAAGTATTG TTGAGAACGACTATGTAAAATATTGT GAGGTTTCTCGCTGGGGTGACCAATGTAGATATGATGTAGAGGAGCACATTTACTATACC ATGAACTGTTCTGACCCAAAGCC

CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGGATGAACATTCCAGAAA GAATTTGAAAAAAGATGAATTCTAG

SEQ ID No. 253 - Pretorisuskop/96/4 MGF 110 7L ATGCTGGTGATTATCCTGGGAATTATTGGCCTGCTGGCCAGTAGCAACCTGGTTTCATCC TCCACTTCTACTCGGGTAGGTGG ACATCTTCCTCTAACATTTGATCCCCCAGAGAATGAACTTGGATACTGGTGCACTTATGT GGAAAGCTGTCGGTTCTGCTGGG ATTGTGAAGATGGGATTTGTACCAGCAGGGTTTGGGGAAACAACTCCACAAGTATTGTTG AGAATGACTATGTAAAATATTGT GAGGTTTCCCGCTGGGGAAACCTATGTAGATATGATGTGGAGGAGCACATTTACTATAGC ATGAACTGTTCTGACCCAAAGCC CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAATATCTCAGAAAGGA TTTGAAAAAAGATGAATTCTAG SEQ ID No. 254 - Tengani 62 MGF 110 7L

ATGAACTGTTCTGACCCAAAGCCCTGGAATCCTTATAAAATTGCAAGGAAGGAGTGG AAAAAGAATGAACATCCCAGAAAGGA T T T GAAAAAAG AT GAAT T C T A G

SEQ ID No. 255 - Warmbaths MGF 110 7L

ATGCTGGTGATTATCCTGGGAATTATTGGCCTGCTGGCCAGTAGCAACCTGGTTTCA TCCTCCACTTCTACTCGGGTAGGTGG ACATCTTCCTCTAACATTTGATCCCCCAGAGAATGAACTTGGATACTGGTGCACTTATGT GGAAAGCTGTCGGTTCTGCTGGG ATTGTGAAGATGGGATTTGTACCAGCAGGGTTTGGGGAAACAACTCCACAAGTATTGTTG AGAATGACTATGTAAAATATTGT GAGGTTTCCCGCTGGGGTAACCTATGTAGATATGATGTGGAGGAGCACATTTACCATAGC ATGAACTGCTCTGACCCAAAGCC CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAACATCCCAGAAAGGA TTTGAAAAAAGATGAATTCTAG

SEQ ID No. 256 - Warthog MGF 110 7L ATGCTGGTGATTATCCTGGGAATTATTGGCCTGCTGGCCAGTAGCAACCTGGTTTCATCC TCCACTTCTACTCGGGTAGGTGG ACATCTTCCTCTAACATTTGATCCCCCCGAGAATGAACTTGGATACTGGTGCACTTATGT GGAAAGCTGTCGGTTCTGCTGGG ATTGTGAAGATGGGATTTGTACCAGCAGGGTTTGGGGAAACAACTCCACAAGTATTGTTG AGAATGACTATGTAAAATATTGT GAGGTTTCCCGCTGGGGTAACCTATGTAGATATGATGTGGAGGAGCACATTTACCATAGC ATGAACTGCTCTGACCCAAAGCC CTGGAATCCTTATAAAATTGCAAGGAAGGAGTGGAAAAAGAATGAACATCCCAGAAAGGA TTTGAAAAAAGATGAATTCTAG

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 110 7L. Suitably the functional version of MGF 110 7L comprises the sequence of SEQ ID No. 247, 248, 249, 250, 251, 252, 253, 254, 255 or 256. Suitably the functional version of MGF 110 7L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 247, 248, 249, 250, 251, 252, 253, 254, 255 or 256. Suitably the functional version of MGF 110 7L consists of the sequence of SEQ ID No. 247, 248, 249, 250, 251, 252, 253, 254, 255 or 256.

MGF 1108L gene sequences

SEQ ID No. 44 - Ken05/Tk1 MGF 110 8L ATGAAGGTGCTGATTCTAGTACTACTGGGGGTCGTTATCCTTCAGGCTGCTCCTATACGT AAATTAGAAGACCTACTGCCAAC CCGTAATCCTCCCCAAAATGAGCTAGTTTACTGGTGCACCTACGCAAACCAATGTGACTT TTGCTGGGAATGTGTACACGGAA TCTGCCGAAACAGAATTCAAGCAGATTGGCCTGTGATTCACCAAAATGACTGGATTATAA ATTGCACCGTTTCCCGCTGGAAT GGTATATGTAGGTATTATGAAGGACCCAGAGTCCATATAGATCATGAAATGGACTGTGCA AATCCAACATCCCATACTTATCC ACACATT GAATACAT GAAGAT CTAT GAAAGAGAT GAC CTAT GA SEQ I D No. 45 - Kenya 1950 MGF 110 8L

ATGAAGGTGCTGATTCTAGTACTACTGGGGGTCGTTATCCTTCAGGCTGCTCCTATA CGTAAATTAGAAGACCTACTGCCAAC

CCGTAATCCTCCCCAAAATGAGTTAGTTTATTGGTGCACCTACGCAAACCAATGTGA TTTTTGCTGGGAATGTATACACGGAA

TCTGCCGAAACAGAATTCAAGCAGATTGGCCTGTGATTCACCAAAATGACTGGATTA TAAATTGCACCGTTTCCCGCTGGAAT GGTATATGTAGGTATTATGAAGGACCCAGAGGCCATATAGATCATGAAATGGACTGTGCA AATCCAACATACCATACTTATCC ACACATT GAATACAT GAAGAT TTAT GTAAGAGAT GAC CTAT GA

SEQ ID No. 46 - Malawi Lil-20/1 (1983) MGF 110 8L

ATGAAGGTGCTGATTCTAGTACTGCTGGCGGTGGTTATCCTTCAAGCCGCTCCTATA CGTAAATTAGAAGATCTACTACCAAC

CCGTTATCCTCCTGACCATGAGCTAGTTTATTGGTGCACCTACGCAAACCAATGTGA CTTTTGCTGGGAATGCGTACACGGTA

TCTGCCGAAATAGGATTCAAGCAGATTGGCCGGTTATTCACCAAAATGACTGGATTA TAAATTGCACGGTTTCCCGCTGGAAT

GGTATATGTAGTTATTATGAAGGACCCAGAAACCATACAGATCATCAAATGGACTGT GCAAATCCAACATCCCATACCTATCC

ACATAGAGAATACATGAAAATTTATGAAAGAGATGACTTATAA

SEQ ID No. 47 - Mkuzi 1979 MGF 110 8L

ATGAAGGTGCTGATTCTAGTACTACTGGGGGGGGGGGGTATTATTCTTCAGGCCGCT CCTATACGTAAATTAGAAGATCTACT ACCAACCCGTTATCCTCCTGAACATGAGCTGGTTTATTGGTGCACCTACGCAAACCAATG TGACTTTTGCTGGGAATGCGTCC ACGGTATTTGCCGAAACAGGATTCAAGCAGATTGGCCGGTTATTCATCAAAATGACTGGA TTATAAATTGCACGGTTTCCCGC TGGAATGGCCAATGTCATTATTATGAAGGTTCTCAACAATATCTACATCATGAAATGGAC TGTACAAATCCAACATCCCATAC CTAT C CACATACAGAATACAT GAAGAT CTAT GAAAGAGAT GAC TTAT GA

SEQ ID No. 48 - Pretorisuskop/96/4 MGF 110 8L

ATGAAGGTGCTAATTCTAGTACTACTGGGGGTGGTTATCCTTCAGGCCGCTCCTATA CGTAAATTAGAAGATCTACTACCAAC CCGTTATCCTCCTGAACATGAGCTAGTTTATTGGTGCACTTACGCAAACCAATGTGACTT TTGCTGGGAATGCGTCCACGGTA TCTGCCGAAACAGGATTCAAGCAGATTGGCCAGTTATTCATCAAAATGACTGGATTATAA ATTGCACGGTTTCCCGCTGGAAT GGTATATGTAGTTATTATGAAGGACCCAGAAACCATACAGATCATCAAATGGACTGTGCA AATCCAACATCCCATACTTATCC ACATAGAGAATACAT GAAGAT CTAT GAAAGAGATGACTTAT GA

SEQ ID No. 49 - Warmbaths MGF 110 8L

ATGAAGGTGCTAATTCTAGTACTACTGGGGGTGGTTATTCTTCAGGCCGCTCCTATA CGTAAATTAGAAGATCTACTACCAAC CCGTTATCCTCCTGAACATGAGCTAGTTTATTGGTGCACTTACGCAAACCAATGTGACTT TTGCTGGGAATGCGTCCACGGTA TCTGCCGAAACAGGATTCAAGCAGATTGGCCAGTTATTCACCAAAATGACTGGATTATAA ATTGCACAGTTTCTCGCTGGAAT GGTATATGTAGTTATTATGAAGGACCCAGAAACCATACAGATCATCAAATGGACTGTGCA AATCCAACATCCCATACTTATCC ACATAGAGAATACAT GAAGAT CTAT GAAAGAGATGACTTAT GA

SEQ ID No. 50 - Warthog MGF 110 8L

ATGAAGGTGCTAATTCTAGTACTACTGGGGGTGGTTATTCTTCAGGCCGCTCCTATA CGTAAAGTAGAAAATCTACTGCCAAC CCGTAATCCTCCCCAAAATGAATTAGTTTATTGGTGCACCTATGCAAACCAATGTGACTT TTGCTGGGAATGCATTCACGGCA TCTGCCGAAACAGGATTCAAGCAGATTGGCCAGTTATTCATCAAAATGACTGGATTATAA ATTGCACGGTTTCCCGCTGGAAT GGCCAATGTCATTATTATGAAGGTTCTCAACAATATCTGCATCATGAAATGGACTGTATA AATCCAACATCCCATACCTATCC ACATACAGAATACAT GAAGAT CTAT GAAAGAGATGACTTAT GA

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 110 8L. Suitably the functional version of MGF 110 8L comprises the sequence of SEQ ID No. 44, 45, 46, 47, 48, 49 or 50. Suitably the functional version of MGF 110 8L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 44, 45, 46, 47, 48, 49 or 50. Suitably the functional version of MGF 110 8L consists of the sequence of SEQ ID No. 44, 45, 46, 47, 48, 49 or 50.

MGF 11012L gene sequences

SEQ ID No. 276 - Benin 97/1 (genotype I) 7643-7332 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTCATTCTTACATAT CAATCACCAACAACCCAGTGGTGTTT

TTATGAAATATCACTTAAAATACTTAATCATCATAGCATGGAAAAATGGAGGGATAA GAATTGGTCAATCATTATAAGGTATT ATTGTTTTTACCTTGTATTTAGCTTTGCATTTGCTGGTTGCGTTGCATTTGCGATCTGCA AAAATCTACGACTGTGTACAACC

ATGAAATTACTTATGCTTTTGAATATTTTGGTTTTGTTATCTCAGCCAATTTTGAAT AATTGA

SEQ ID No. 277 - China/2018/AnhuiXCGQ (genotype II) 14155-13796 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTTATTCTTACCTAT CAATCACCAACAACCCCGTGGTGTTT

TTATGAAATATCACTTAAAATACCTAATCATCATAGCATGAAATGTTGTTCGTATCC TAGACTTTATGAACATGAAATGTTCA

TGGAAAAATGGAGGGATAAGAATTGGCCAATCATTATAAGGTATTATTGTTTTTACC TTGTATTTAGCTTTGTATTTGCTGGT

TGCGTTGCATTTGCGATCTGCAAAAATCTACGACTGAGTACAACCATGAAATTACTT ATGCTTTTGAGTATTTTGGTTTTGTT

GTTATCTCAGCCAATCTTGAATAATTGA

SEQ ID No. 278 - Georgia 2007/1 (genotype II) 14151-13792 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTTATTCTTACCTAT CAATCACCAACAACCCCGTGGTGTTT

TTATGAAATATCACTTAAAATACCTAATCATCATAGCATGAAATGTTGTTCGTATCC TAGACTTTATGAACATGAAATGTTCA

TGGAAAAATGGAGGGATAAGAATTGGCCAATCATTATAAGGTATTATTGTTTTTACC TTGTATTTAGCTTTGTATTTGCTGGT

TGCGTTGCATTTGCGATCTGCAAAAATCTACGACTGAGTACAACCATGAAATTACTT ATGCTTTTGAGTATTTTGGTTTTGTT

GTTATCTCAGCCAATCTTGAATAATTGA

SEQ ID No. 279 - Ken05/Tk1 (genotype IX) 15456-14950 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTTTTCAATCATCCTCATTCTTACATAT CAATCACCGACAACTCAGCATCCTCC

TAAGGAAGAGCTTGCGTACTGGTGCACTTATGCAAAATCTTGTGACTTCTGCTGGGA TTGCCAAAATGACACTTGTATAAATA

AGGTAATAAATGAGTCTATTTCGATAACTTCGATTGTAAACTGTAGAGTTACTCGCG ATTCCCAATCCTGTTTTTATGAAATA

TCAGTTAAAATACCTAATCATCATAGCATGGAATGTTCATATCCTAGACTTTATGAA CATGAAATGTTTATGGAAAAATGGAG

GGATGAGTATTGGCCAATCATTATAAAACAGTGTTGTTTTTACCTTGTATTTAGCAT TGCATTTGCTGGATGCGTTGCATTTG

CGATCTGCAAAAATCTACGACTGCGTACAACTATAAAACTACTTATTCTTTTGAGTA TTTTGGTTTGGTTGTCTCAACCAGTC

TTGAATTGA

SEQ ID No. 280 - Ken06.Bus (genotype X) 11961-11446 MGF 110 12L

ATGAAGGTTTTTCTGGCACTTTTACTAGGTTATTTAACTATCCTCATTCTTACATAT CAAACACCAACAACCCAGCATCCTCC TAAGGAGGAGCTTCCATATTGGTGTACGTATGTAAAGAATTGCGACCTCTGTTGGGATTG TCAAGATAGCATCTATTGGAACA AGGTAATAAGTGAGTCTATTTCGATAAATTCGATTATAAACTGTAGAGTTACTTGCGATT CCCAATCTCAGTCCTGCTTTTAT GAAATATTACTTAAAATACCTAATCATCATAGCATGGAATGTTCTTATCCTGGATCGTAT GAAAATGAAATGTTCATGGAAAA ATGGAGGGATGAGAATTGGTCAATCATTATAAAACATTATTGTTTTTACCTTGTATTTAG CTTTGCATTTGCTGGTTGCGTTG CATTTGCGATCTGCAAAAATCTACGACTGAGTACAACTATGAAATTACTTATGCTTTTGA GTATTTTGGTTTGCTTATCTCAG C CAAT CT T GAATAAT T GA

SEQ ID No. 281 - Kenya 1950 (genotype X) 16240-15731 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTTTTCAATCATCCTCATTCTTACATAT CAATCACCGACAACCCAGCATCCTCC TAAGGAAGAGCTTGCGTACTGGTGCACTTATGCAAAATCTTGTGACTTCTGCTGGGATTG CCAAAATGACACTTGTATAAATA AGGTAATAAATGAGTCTATTTCGATAACTTCGATTGTAAACTGTAGAGTTACTCGCGATT CCCAGTCCTGTTTTTATGATATA TCAGTTAAAATACCTAATCATCATAGCATGGAATGTTCATATCCTAGACTTTATGAACAT GAAATGTTTATGGAAAAATGGAG GGATGAGTATTGGCCAATCATTATAAAACAGTGTTGTTTTTACCTTGTATTTAGCTTTGC ATTTGCTGGTTGCGTTGCATTTG CGATCTGCAAAAACCTACGACTGCGTACAACTATAAAACTACTTATTCTTTTGAGTATTT TGGTTTGGTTATCTCAGCCAATC TT GAATAAT TGA

SEQ ID No. 282 - L60 (genotype I) 7993-7682 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTCATTCTTACATAT CAATCACCAACAACCCAGTGGTGTTT

TTATGAAATATCACTTAAAATACTTAATCATCATAGCATGGAAAAATGGAGGGATAA GAATTGGTCAATCATTATAAGGTATT

ATTGTTTTTACCTTGTATTTAGCTTTGCATTTGCTGGTTGCGTTGCATTTGCGATCT GCAAAAATCTACGACTGTGTACAACC

ATGAAATTACTTATGCTTTTGAATATTTTGGTTTTGTTATCTCAGCCAATTTTGAAT AATTGA

SEQ ID No. 283 - Malawi Lil-20/1/1983 (genotype VIII) 12584-12069 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTCATTCTTACATAT CAATCACCAACAACCCAGCATCCTCC

TAAGGAAGAGCTTGAGTATTGGTGCACTTATGCAAAAACTTGTGACTTCTGCTGGGA TTGCCAAAATGACACTTGTATAAATA

AGGTAATAAATGAATCTATTTCGATGAATTCGATTGTAAACTGTAGAGTTACTCGCG ATTCCCAATCCCAGTCCTGTTTTTAT GAAATATCACTTAAAATACCTAATTATCATAGCATGGAATGTTCATATCCTAGACTTTAT AAACATTTCATGTCCATGGAAAA ATGGAGGGATGAGAATTGGCCAATCCTTATAAGACATTATTGTTTTTACCTTGTATTTAG CTTTGCATTTGCTGGTTGCGTTG CATTTGCGATCTGCAAAAATCTACGACTGCGTACAACCATGAAATTACTTATGCTTTTGA GTATTTTGGTTTTGTTATCTCAG C CAAT CT T GAATAAT T GA

SEQ ID No. 284 - Mkuzi 1979 (genotype XII) 15894-15571 MGF 110 12L

ATGAACGCAACCATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTC ATTCTTACATATCAATCACCGACAAC CCAGTGGTGTTTTTATGAAATATCACTTAAAATACTTAATCATCATAGCATGGAAAAATG GAGGGATAAGAATTGGTCAATCA TTATAAGGTATTATTGTTTTTACCTTGTGTTTAGCTTTGCATTTGCCGGTTGCGTTGCAT TTGCGATCTGCAAAAATCTACGA CTGTGTACAACCATGAAATTACTTATGCTTTTGGGTATTTTGGTTTTGTTATCTCAGCCA ATCTTGAATAATTGA

SEQ ID No. 285 - Warmbaths (genotype lll/l) 14212-13859 MGF 110 12L

ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTCATTCTTACATAT CAATCACCAGCAACCCAGTGGTGTTT TTATGAAATATCACTTAAAATACCTAATCATCATAGCATGGAATGTTCATATCCTAGACT TTATAAACATTTCATATTCATGG AAAAATGGAGGGATAAGAATTGGTCAATCATTATAAGGTATTATTGTTTTTACCTTGTAT TTAGTTTTGCATTTGCTGGTTGC ATTGCATTTGCGATCTGCAAAAATCTACGACTGTGTACAACTATGAAATTACTTATGCTT TTGAGTATTTTGGTTTTGTTATC T CAGC CAAT CT T GAATAAT T GA

SEQ ID No. 286 - Warthog (genotype IV) 12897-12544 MGF 110 12L ATGAAGGTTTTTCTAGGACTTTTACTAGGTTATTCAACCATCCTCATTCTTACATATCAA TCACCAGCAACCCAGTGGTGTTT TTATGAAATATCACTTAAAATACCTAATCATCATAGCATGGAATGCTCATATCCTAGACT TTATAAACATTTCATGTTCATGA AAAAATGGAGGGATAAGAATTGGTCAATCATTATAAGGTATTATTGTTTTTACCTTGTAT TTAGTTTTGCATTTGCTGGTTGC ATTGCATTTGCGATCTGCAAAAATCTACGACTGTGTACAACTATGAAATTACTTATGCTT TTGAGTATTTTGGTTTTGTTATC T CAGC CAAT CT T GAATAAT T GA

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 110 12L. Suitably the functional version of MGF 110 8L comprises the sequence of SEQ ID No. 276, 277, 278, 279, 280, 281, 282, 283, 284, 285 or 286. Suitably the functional version of MGF 110 12L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 276, 277, 278, 279, 280, 281, 282, 283, 284,

285 or 286. Suitably the functional version of MGF 110 12L consists of the sequence of SEQ ID No. 276, 277, 278, 279, 280, 281, 282, 283, 284, 285 or 286.

MGF 3606L gene sequences

SEQ ID No. 51 - Georgia 2007/1 MGF 3606L (NC_044959.1:17222-18349) TTACCGCAAATTGTCTCTCAGCGAAGAAAATGAATGAAACGTTTCTGTATATTCATAGGT TGAAATTATTTTACGCACTTCAC TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAAAAA GAAGTTTCATCATAACATTCTTT TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTTATA CAAAAATGTACATTACTCCAAAT ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACCTATCAA CGTCTCTAAGGCCGTATTGATAT TTGCGCCTAAGGCAAAACAATAGTATATACCCAATTTATTTTGAGGGTACATACAAGCAA GCGACATCATGTCATTTGGATCT AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTATACA GCCGTTTTTAAACGCCAATAATC TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTATGGC GATCTTAAACCAAAATTTTAATA CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACGATTGTAAA TAAAGTCTATCAACACGTAAAAA TCATGGCTATCAAAACTGTCGAGAATCGAAATATTGTCATAATAAATATCTATAGCTAAT AAGACCTTTTGTTGTTTAATTAG ATCAACAAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTGAAT ACACAGAACTTTCGTCCTTTCCG TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAATAACTTGATTA GATCAGTCTGGTTTTTCTTCACA GCCCTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACATCAGAACATGACATAGAGCCA CTGGTTACCATTTTACATTGTTT ACAAAAACCTATGGGTCCGTTTTCCCACCATAATCCAAGCTGCTGTAAAATAAAAATATC ATCCTCATGATAATTTGAAAAAG CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 52 - China/2018/AnhuiXCGQ MGF 3606L (MK128995.1:17221-18348) TTACCGCAAATTGTCTCTCAGCGAAGAAAATGAATGAAACGTTTCTGTATATTCATAGGT TGAAATTATTTTACGCACTTCAC

TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGAAGTTTCATCATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACCTAT CAACGTCTCTAAGGCCGTATTGATAT

TTGCGCCTAAGGCAAAACAATAGTATATACCCAATTTATTTTGAGGGTACATACAAG CAAGCGACATCATGTCATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATAATC

TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACGATTGT AAATAAAGTCTATCAACACGTAAAAA

TCATGGCTATCAAAACTGTCGAGAATCGAAATATTGTCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAATTAG

ATCAACAAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTG AATACACAGAACTTTCGTCCTTTCCG

TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAATAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACATCAGAACATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGTCCGTTTTCCCACCATAATCCAAGCTGCTGTAAAATAAAAAT ATCATCCTCATGATAATTTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 53 - Warmbaths MGF 3606L (AY261365.1:17236-18363

TTACCGCAAATTGTCTCTCAGTAAAGAAAATGAATGAAACGTTTCTGTATATTCATA GGTTGAAATTATTTTACGCACTTCAC

CAGGTTCTAATATTTTTTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGAAGTTTCATTATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACCTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCACCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAG CAAGCGGCATCATGTCATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATAATC

TAGGTAAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACAATTGT AAATAAAGTCTATCAACACGTAAAAA

TCATGGCTATCAAACCTGTCGAGAATCGAAATATTATCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAATTAG

ATCAACAAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTG AATACACAGAGCTTTCATCCTTTCTG

TCTTGGCGCATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAATAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGCG CCACTAGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGACCGTTTTCCCACCATAGTCCAAGCTGCTGTAAAATAAAAAT ATCATCTTCATGATATTTTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ I D No. 54 - L60 MGF 3606L (NC_044941.1 : 11041 -12168)

TTACCGCAAATTGTCTCTCAGCAAAGAAAATGAATGAAACGTTTCCGTATATTCATA GGTTGAAATTATTTTACGCACTTCAC

TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGAAGTTTCATTATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACCTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCGCCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAG CAAGCGACATCATGTCATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATAATC

TAGATGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACAATTGT AAATAAAGTCTATCAATACGTAAAAA

TCATGGCTATCAAAACTGTCGAGAATCGAAATATTATCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAATTAG

ATCAACAAACATATTATACAATCCTACATCTAAAAATTCTGGATTGGCTCCTAGTTC AATACACAGGTCTTTCGTCCTTTCCG

TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAACAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTTACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGGCCATTATGCCACCACAATCCAAGCTGCTGTAAAATAAAAAT ATCATCCTCATGATATTCTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 55 - Benin 97/1 MGF 3606L (NC_044956.1 :10692-11819)

TTACCGCAAATTGTCTCTCAGCAAAGAAAATGAATGAAACGTTTCCGTATATTCATA GGTTGAAATTATTTTACGCACTTCAC

TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGAAGTTTCATTATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACCTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCGCCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAG CAAGCGACATCATGTCATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATAATC

TAGATGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACAATTGT AAATAAAGTCTATCAATACGTAAAAA

TCATGGCTATCAAAACTGTCGAGAATCGAAATATTATCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAATTAG

ATCAACAAACATATTATACAATCCTACATCTAAAAATTCTGGATTGGCTCCTAGTTC AATACACAGGTCTTTCGTCCTTTCCG

TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAACAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTTACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGGCCATTATGCCACCACAATCCAAGCTGCTGTAAAATAAAAAT ATCATCCTCATGATATTCTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT SEQ ID No. 56 - Pretorisuskop/96/4 MGF 3606L (AY261363.1 :17123-18250)

TTACCGCAAATTGTCTCTCAGCAAAGAAAATGAATGAAACGTTTCCGTATATTCGTA GGTTGAAATTATTTTACGCACTTCAC

TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCCTAA AAAGAAGTTTCATTATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCCATTTACTTCATGATTAATGTAACGTAT CAATGTCTCTAAGGCAGTATTGATAT

TTGCGCCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAG CAAGCGGCATCATGTCATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATCATC

TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CATATGTATTTTTTATCATTTTTTCTTTTTCATCTAAATTTAAGATAAAACAATTGT AAATAAAGTCTATCAACACGTAAAAA

TCATGACTATCAAACCTGTCGAGAATCGAAATATTATCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAATTAG

ATCAACAAACAAATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTG GATACATAGAACTTTCGTCCTTTCCG

TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAACAAATAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGGCCATTATGCCACCATAGTCCAAGCTGCTGTAAAATAAAAAT ATCATCTTCATGATAATTTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 57 - Mkuzi 1979 MGF 3606L (AY261362.1 : 18925-20052)

TTACTGCAAATTGTCTCTCAGCAAAGAAAATGAATGAAACGTTTCTGTATATTCATA GGTTGAAATTATTTTACGCACTTCAT

TAGGTTCTATTATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGAAGTTTCATTATAACATTCTTT

TCTTGTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTCCTTCATGATTAATGAAACCTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCGCCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAG CAAGCGACATCATGTCATTTGGATCT

AAACGTATATTTTCTTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATCATC

TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTGTAT GGCGAACTTAAACCAAAATTTTAATA

CAAGTGTATTTTTTGTCATTTCTTCTTTTTCATCTAAGTATAGGATAAAACGATTGT ACATAAAGTCTATCAACACGTAAAAA

TCATGGCTATCAAAACTGTCGAGAATCGAAACATTATCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAAGTAG

ATCAACAAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTG AATACACAGAACTTTCGTCCTTTCCG

TCTTGGCACATATGATGCCATAATTAATGTTGGCACCCCATAAAATAAATAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCCTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGGCCATTATGCCACCATAGTCCAAGCTGCTGTAAAATAAAAAT ATCATCTTCATGATATTCTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 58 - Ken05/Tk1 MGF 3606L (NC_044945.1 : 19166-20293)

TTATCGTAAATTGTCTCTTAGCGAAGAAAATGAATGAACCGTTTCCGTATATTGATA GGTTGAAATTATTTTACGCACTTCAC

TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAA AAAGTAGTTTCATTATAACATTCTTT

TCTTCTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACGTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCCCCTAAGGCAAAACAATAGTATATACCTAACTTATTTTGAGGGTACATACAAG CAAGCGACATCATGTTATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCGTTTTTAAACGCCAATGATC

TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT CGCGAACTTATACCAAAATTTTAATA

CAAGTGTATTTCTTGTCATTTCTTCTTTTTCATCTAAATTTAAGATAAAACGATTGT AAATAAAGTCTATCAACGTGTAAAAA

TCATGGTCATCAAAACTATCGAGAATCGAAATATTATCATAATAAATATCTATAGCT AATAAGACTTTTTGTTGTTTAAGTAG

ATCAACAAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTG AAGACACAGAACTTTTGTCCTTTCCG

TCTTGGCACACATAATGCCATAATTAACGTTGGCACCCCATAAAACAAATAACTTGA TTAGATCAGTCTGGTTTTTCTTCACA

GCCGTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACATCAGAGCATGACATAGAG CCACTGGTTACCATTTTACATTGTTT

ACAAAAACCTATGGGGCCATCATGCCACCACAACCCTAGCTGCTGTAAAATAAAAAT ATCATCCTCATGATAATTTGAAAAGG

CCTTGGTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

SEQ ID No. 59 - Malawi Lil-20/1 (1983) MGF 3606L (AY261361.1:15478- 16605)

TTACCGCAAATTGTCTCTCAGCGAAGAAAATGAATGAAACGTTTCCGTATATTCATA GGTTGAAATTATTTTACGCACTTCAC

CAGGTTCTAATATTTTTTTATGAAGTATTGAATGGGAGGTTAAAAGTCCTTTCTTAA AAAAGAGTTTCATTATAACATTCTTT

TCTTCTCTAAGAAGAGTTTCTTGTATTTTTTTTATATAAGGATTGGCACCCAAACTT ATACAAAAATGTACATTACTCCAAAT

ACCATAATTTGAAAAGAAAGTTATTTCCCTATCTACTTCATGATTAATGAAACGTAT CAACGTCTCTAAGGCAGTATTGATAT

TTGCACCTAAGGCAAAACAATAGTATATACCTAATTTATTTTGAGGGTACATACAAG CAAGCGACATCATGTGATTTGGATCT

AAACGTATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTACCTAAGTAT ACAGCCATTTTTAAACGCCAATCATC

TAGGTGAGGAAATTTCTTACTAAGAAAACGAATAGGTTTTATAAGATTAAACTCTAT GGCGATCTTAAACCAAAATTTTAATA

CAAGTGTATTTCTTGTCATTTCTTCTTTTTCATCTAAGTATAGGATATAACGATTGT AAATAAAGTTTATCAATGCGTAAAAA

TCATGGCTATCAAAACTGTCGAGAATGGGAATGTTTTCATAATAAATATCTATAGCT AATAAGACCTTTTGTTGTTTAAATAG

ATCAACAAACATAGTATACAACCCTACCTCTAAAAATGTTGGATCAGCTCCTAGTTG AATACACAGAACTTTCGTCCTTTCCA

TCTTGGCACATATGATGCCATAATTGATGTTGGCACCCCATAAAACAAATAACTTGA TTAGATCAGTCTGATTTTTCTTTACA GCCTTCACCAAGGCTCTGTCAAGCTCATAGCTGTCAACGTCAGAGCATGACATAGAGCCA CTGGTTACCATTTTACATTGTTT

ACAAAAACCTATAGGTCCGTTTTCCCACCATAATTCTAGCTGCTGTAAAATAAAAAC ATCATCTTCATGATAATTTGAAAAAG

CCTTGTTTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT SEQ ID No. 60 - Kenya 1950 MGF 3606L (AY261360.1:19341 -20462)

TTACCGCACATTGTCTCTCAGCGAAGAAAATGAATGAAACGTTTCCGTATATTGATA GGTTGAAATTATTTTACGCACTTCAC TAGGTTCTAATATTTTCTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTCTTAAAAA GTAGTTTCATTATATCATACTTT TCTTCTCTAAGAAGAATTTCTTGTATTTTTTTTGTATAAGGATTGGCACCCAAACTTATA CAAAAATGTACATTACTCCAAAT ACCATAATTTGAAAAGAAAGGTCTATTTAGTTCATGATTAATGAAACGTATCAACGCCTC TAAGGCAGTATTGATATTTGCCC CTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGATACATACAAGCAAGCGACA TCATGTGATTTGGATCTAAACGT ATATTTTCCTGAAAATATGCATGATGGATTTCATCAACATTCCCTAAGTATACAGCCGTT TTTAAACGCCAATCATCTAGGTG AGGAAATTTCTTACCAAGAAAACGAATAGGTTTTACAAGATTAAACTCTATGGCAAACTT ATACCAAAATTTTAATACAAGTG TATTTTTTGTTATTTTTTCTTTTTCATCTAAATTTAAGATAAAACCATTGTAAATAAAGT CTATCAACACGTAAAAATCATGG CTATCAAAACTGTCGAGAATCGAAATATTATCATAATAAATATCTATAGCTAATAAGACC TTTTGTTGTTTAATTAGATCAAC AAACATATTATACAACCCTACATCTAAAAATTTTGGATCAGCTCCTAGTTGAATACATAG AACTTTCGTCCTTTCCGTCTTGG CACACACGATGCCAAAATTAATTTCGGCGCCCCATAAAACAAATAACTTGATTAGATCAG TTTGGTTTTCCTTCACAGCTTTT ACTAAGGCTCTGTCAAGCTCATAGCTGTCGACATCAGAACATGACATAGAGCCACCGGTT ACCATTTTACATTGTTTACAAAA ACCTATGGGTCCGTTTTCCCACCACAGTCCAAGCTGCTGTAAAATGAAACTATCATCTTC ATGATAATTTGAAAAAGCCTTGT TTTCTATCAAGACTTTTTTTGTAAGAACCTGTAAAGAGTTCAT

SEQ ID No. 61 - Ken06.Bus MGF 3606L (NC_044946.1 :15081-16208)

TTACCGCAAATTATCTCTCAGTGAAGAAAATGAATGAAACGTTTCCGTATGTTCATA GGTTGAAATTATTTTACGCACCTCAC TAGGTTCTAATATTTTTTTATGAAGTATTGAATGGGGGCTTAAAAGTCCTTTTTTAAAAA GTAGTTTCATTATAACATTCTTT TCTTCTCTAAGAAGAGTTTCTTGTATTTTTTTTGTATCAGGGTTGGCACCCAAACTTATA CAAAAATGTATATTACTCCAAAT ACCATAATTTGAAAAGAAAGTTATTTCCCTATTTACTTCATGATTAATGAAACGTATCAA CGTCTCTAAGGCAGTATTGATAT TTGCGCCTAAGGCAAAACAATAGTATATACCTAATTTATTCTGAGGGTACATACAAGCAA ACGACATCATGTGATTTGGATCT AAACGTATATTTTCTTGAAAATATGCATGATGGATTTCATCAACATTCCCTAAGTATACA GCCGTTTTTAAACGCCAATCATC TAGGTGAGGAAATTTCTTACTAAGATAACGGATAGGTTTTACAAGATTAAACTCTATGGC GAACTTAAACCAAAATTTTAATA CAAATGTGTTTCTTGTCATTTTTTCTTTTTCATCTAAATTTAGGATAAAACGATTGTAAA TAAAGTTTATCAACGCGTGAAAA TCATGGCTATTAAAGCTGTCGAGAATCAAAATATTATCATAATAAATATCTATGGCTAAT AAGACCTTTTGTTGTTTAATTAG ATTAACAAACATATTATACAGCTTTTTATCTAAAAATTCTGGATCGGCTCCTAGTTGAAT ACACAGGTCTTTCGTCCTTTCCA TCTTAGCACACATAATGCCATAATTAATGTTGGCACCCCATAAAACAAATAATATGATTA GATCAGTTTGGTTTTCCTTCACA GCTTTTACTAAGGCCCTGTCAAGCTCATAGCTGTCAACATCAGAGCATGACATTGAGCCA ATGGTTACTATTTTACATTGTTT ACAAAAACCTATGGGGCCGTTTTCCCACCACAATCCTAGCTGCTGTAAAATAAAACTATC ATCTTCATGATAATTTGAAAAGG CCTTGTTTTCTATTAAGACTTTTTTTGTAAGAACCTGTAAAGAATTCAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 3606L. Suitably the functional version of MGF 360 6L comprises the sequence of SEQ ID No. 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 or 61. Suitably the functional version of MGF 360 6L comprises a sequence having at least 70%, at least 80%, at least 90% or at least

95% identity with SEQ ID No. 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 or 61. Suitably the functional version of MGF 360 6L consists of the sequence of SEQ ID No. 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 or 61.

MGF 36010L gene sequences SEQ ID No. 62 - Georgia 2007/1 MGF 360 10L (LR743116.1 :26373-27410)

CTAGGCCAACATACTTTTTAATTTATAGTTTTTTAATAGATGATATATTTTGTTAGG ATCTGCTTCTTTTAACGTTAATAGCG AGGAGTCTGCACTATAAATGTCTAATGATAAATGATGAGATATCAAATAGTAATTCCGTT GCTCTGCTAGGGCCTTTGCCTCT TCAAAGGCGTCGGCTCCCAGATCTATACAAAAGAACAAGTTATCCATATTATAAAATCGT ACGCAGGCAAGCATAGCTGAATT AATATTAGCTCCTAAGAGAAAACAATAATATATGGTTAAAAAATTGTTATCTTTTGTGCA GGCCATCCGCATCATTTCATCCA CGTCCATGCGGATCTTTTCCTTTTCATACAAATTATGTAGGTCAAACAGCTTATTAAAAC AAAGAGCACAGATTAACCACCAC GTATTTAGATACTTAAAATGTTGGTAAACATAAGAAATGGCCTCCCTAAGATTATCCTGC AATGCCACTATAAAACAGTATAT CGTTAACATATCACCATCCGACATATTACTTAATATGTCGGTGTCTTCTACTAACCTTTT CAACTTCCAATATATGGATGACC TTATTTCCCTTATAATGACATAGGCTGGAAAGGGATTATCATTAAAAAGTTTAAGACATA AGATAATATTACTGCTAGTAGTG CCAGGGTGTATTAATTTAAAGAACATGTGCATAATCTTCTTTTTATCCACGCGGTACTTG GCTCCTAATTCCCAGCAAAATTC TCGAACAGGCGGCGTATTGGCGCAAATTAACCCATAGTTGATGTCTGCGCCCCATTCTGT AAACAGTTTTATTAACTGATAGT

TGTTTTCCTTTGTAGCCAACATTAGTGCCGTATTAAGGTCCAAGCCGTCTGCAAAGC TTGGCAGCTTTATCAGCATATGTTTG

CAATCAAGGGAAATTGGGGCCTTATACCACCATAGTCCGCAGCGTTCTAAGATAACA TGGTACTCAATAGATACTTGCTGTCT

GGCTAGTACCTTTTTGGCGAAGGATTGTAAGGAAGGAAACAT

SEQ ID No. 63 - China/2018/AnhuiXCGQ MGF 360 10L (MK128995.1:25402-26439)

CTAGGCCAACATACTTTTTAATTTATAGTTTTTTAATAGATGATATATTTTGCTAGG ATCTGCTTCTTTTAACGTTAATAGCG

AGGAGTCTGCACTATAAATGTCTAATGATAAATGATGAGATATCAAATAGTAATTCC GTTGCTCTGCTAGGGCCTTTGCCTCT

TCAAAGGCGTCGGCTCCCAGATCTATACAAAAGAACAAGTTATCCATATTATAAAAT CGTACGCAGGCAAGCATAGCTGAATT

AATATTAGCTCCTAAGAGAAAACAATAATATATGGTTAAAAAATTGTTATCTTTTGT GCAGGCCATCCGCATCATTTCATCCA

CGTCCATGCGGATCTTTTCCTTTTCATACAAATTATGTAGGTCAAACAGCTTATTAA AACAAAGAGCACAGATTAACCACCAC

GTATTTAGATACTTAAAATGTTGGTAAACATAAGAAATGGCCTCCCTAAGATTATCC TGCAATGCCACTATAAAACAGTATAT

CGTTAACATATCACCATCCGACATATTACTTAATATGTCGGTGTCTTCTACTAACCT TTTCAACTTCCAATATATGGATGACC

TTATTTCCCTTATAATGACATAGGCTGGAAAGGGATTATCATTAAAAAGTTTAAGAC ATAAGATAATATTACTGCTAGTAGTG

CCAGGGTGTATTAATTTAAAGAACATGTGCATAATCTTCTTTTTATCCACGCGGTAC TTGGCTCCTAATTCCCAGCAAAATTC

TCGAACAGGCGGCGTATTGGCGCAAATTAACCCATAGTTGATGTCTGCGCCCCATTC TGTAAACAGTTTTATTAACTGATAGT

TGTTTTCCTTTGTAGCCAACATTAGTGCCGTATTAAGGTCCAAGCCGTCTGCAAAGC TTGGCAGCTTTATCAGCATATGTTTG

CAATCAAGGGAAATTGGGGCCTTATACCACCATAGTCCGCAGCGTTCTAAGATAACA TGGTACTCAATAGATACTTGCTGTCT

GGCTAGTACCTTTTTGGCGAAGGATTGTAAGGAAGGAAACAT

SEQ ID No. 64 - Ken06.Bus MGF 360 10L (NC_044946.1:23437-24507)

TTACGACGTTGTATCATTGATGTGATAATCCAAATAGGCCAACATACTTTTTGATTT ATAGTTGTTTAATAGATGGTATATTT

TTTTAGGGTTCGCTTCTTTTAGCGTGAATAGAGAGGGATCTGGATGATAGATATCTA ATGACAAACGTTGTGATATTAAATAA

TAGTCCCGCTGCTCTGCTAGGGCTTTTGCCTCTTCGAAGGCATTGGCTCCCAAGTCT ATACAAAAGAACAGATTATCTATATT

AAAGAACTGTATGGCAGCAAGCATGGCCATATTGATATCGGCCCCTAAAATAAAACA ATAATACATCGTTAAAAAGTTATTAT

CTTTCGTGCAAGCTATGCGCATCATTTCATTAATGTCTATGCGAATCTTTTCCTGCT CATAGAGATAATGGAGGTCAGACAGT

TTATTAAAATACAGAGCACAGATTAACCACCACGTATTCATGTGCTTAAAATGTTCG TAAAAATACGAGACGGCCTCCCTAAG

ATCATTCTGCAAGGCCACCATAAAACAATATATAGTTAGCATATCGCCGTCCGAAAC ATCACGTAGTAGGTCTTCATCTTCTA

CTAACCTCCTCAGCCTCCAATAAAGAGACGACTTTATTTCCCTTATAATCACATAGG TAGGAAAAGGATTCTCATTAAAAAGC

TTATGACATAAGATAATATATTGACTTGTTTTGTTCTCTATAAATTTAAAGAACATG TGTATCACCTTTTTTTTATCCATGCG

ATAGTTCGCTCCTAATTCCCAACAAAACTCTCGGGTAGGCTGTGTATTGGCACAAAT GTATCCATAGTTGATGTTTGCACCCC

ACTCCGTGAATAGTTTTATTAATTGATAGTTGTTTTCCTTTGCAGCTATCATCAATG CCGTATTGAGATCCAGGCCATCCGCA

AAGTATGGCAACCTTATTAACATGTGTTTGCAATCAAGTGAAATTGGAGCCGTATAC CACCATAACCCGCAGTATTTCAAAAT

ATGATAGTAGTCTTCAGGCAGCACATGCTGACTGGCTAATATCTTTTTGGCGAAAGA CTGTAAAGAAGGAAACAT

SEQ ID No. 65 - Ken05/Tk1 MGF 360 10L (NC_044945.1 :27310-28380)

TTACGACGTTGTATCATTATTGTGATAATCCAAATAGGCCGACATACTTTTTGATTT ATAATTGTTTAATAGATGGTATATTT

TTTTAGGGTTCGCTTCTTTTAGCGTGAATAGAGAGGGATCTGGATGATAGATATCTA ATGACAAACGATGTGATATTAAATAA

TAGTCCCGCTGCTCTGCTAGGGCTTTTGCCTCTTCGAAGGCATTGGCCCCCAAGTCT ATACAAAAGAACAGATTATCTATATT

AAAAAACTGTATGGCAGCAAGCATCGCCATATTGATATCGGCCCCTAAAATAAAACA ATAATACATCGTTAAAAAGTTGTTAT

CTTTCGTGCAAGCTATGCGCATCATTTCATTAATGTCTATGCGAATTTTTTCCTGCT CATAGAGATAATGGAGGTCAAACAGT

TTATTAAAACACAGAGCACAGATTAACCACCACGTATTCAGGTGCTTAAAATGTTGG TAAAAATACGAGACGGCCTCCCTAAG

ACCATTCTGCAAGGCCACCATAAAACAATATAAAGTTAGCATATCGCCGTCCGAAAC ATCGCGTAGTAGGTCTTCGTCTTCTA

CTAACCTCCTCAGCCTCCAATAAAGAGACGACTTTATTTCCCTTATAATCACATAGG TAGGAAAAGGATTCTCATTAAAAAGC

TTATGACATAAGATAATATATTGACTCGTTTTGTTCTCTATAAATTTAAAAAACATA TGCATCACCTTTTTTTTATCTAAGCG

ATAGTTCGCTCCTAATTCCCAACAAAACTCTCGAGTAGGCTGTGTATTGGCACAAAT GTATCCATAGTTGATGTTTGCACCCC

ACTCCGTGAACAGTTTTATTAATTGATAGTTGTTTTCCTTTGCAGCTATCATCAATG CCGTATTGAGGTCCAGGCCATCCGCA

AAGTATGGCAACCTTATTAACATGTGTTTACAATCAAGTGAAATTGGAGCCTTATAC CACCATAACCCGCAGCGCTTCAAAAT

ATGATAGTAGTCTTCAGGCAGCACATGCTGGCTGGCTAATATCTTTTTGGCGAAAGA TTGCAGGGAAGGAAACAT

SEQ ID No. 66 - Kenya 1950 MGF 360 10L (AY261360.1 :29558-30628)

TTACGACGTTGTATCATTAATGTGATAATCCAAATAGGCCGACATACTTTTTGATTT ATAGTTGTTTAATAGATGGTATATTT

TTTTAGGGTTCGCTTCTTTTAGCGTGAATAGAGAGGGATCTGGATGATAGATATCTA ATGACAAACGATGTGATATTAAATAA

TAGTCCCGCTGCTCTGCTAGGGCTTTTGCCTCTTCGAAGGCATTGGCCCCCAAGTCT ATACAAAAGAACAGATTATCCATATT

AAAGAACTGTATGGCAGCAAGCATGGCCATATTGATATCGGCCCCTAAAATAAAACA ATAATACATCGTTAAAAAGTTGTTAT

CTTTCGTGCAAGCTATGCGCATCATTTCATTAATGTCTATGCGAATTTTTTCCTGCT CATAGAGATAATGGAGGTCAAACAGT

TTATTAAAACACAGAGCACAGATTAACCACCACGTATTCAGGTGCTTAAAATGTTGG TAAAAATACGAGACGGCCTCCCTAAG

ATCATTCTGCAAGGCCACCATAAAACAATATAAAGTTAGCATATCGTCGTCCGAAAC ATCGCGTAGTAGGTCTTCGTCTTCTA

CTAATCTCCTCAGCCTCCAATAAAGAGACGACTTTATTTCCCTTATAATCACATAGG TAGGAAAAGGATTCTCATTAAAAAGC

TTATGACATAAGATAATATACTGACTCGTTTTGTTCTCTATAAATTTAAAAAACATA TGCATCACCTTTTTTTTATCTAAGCG

ATAGTTTGCTCCTAATTCCCAACAAAACTCTCGAGTAGGCTGTGTATTGGCACAAAT GTATCCATAGTTGATGTTTGCACCCC ACTCCGTGAACAGTTTTATTAATTGATAGTTGTTTTCCTTTGCAGCTATCATCAATGCCG TATTGAGGTCCAGGCCATCCGCA

AAGTATGGCAACCTTATTAACATGTGTTTACAATCAAGTGAAATTGGAGCCTTATAC CACCATAACCCGCAGCGCTTCAAAAT

ATGATAGTAGTCTTCAGGCAGCACATGCTGGCTGGCTAATATCTTTTTGGCGAAAGA TTGCAGGGAAGGAAACAT

SEQ ID No. 67 - L60 MGF 360 10L (NC_044941.1:19956-21026)

T TATAAC GAT G TAT CAT T GAT GT CATAAT T C AAAT AG G C CAACAT AC t T T T T GAT T TAT AG t T T T T T AATAGAC GAT AT AT T T TGTTAGGATCTGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATGTCTAATG ATAAACGATGAGATATCAAATAG TAATTCTGTTGCTCTGCCAAGGCTTTTGCCTCTTCAAAGGCATCGGCCCCCAGATCTATA CAAAAGAACAGGTTATCCATATT ATAGAATCGTATGGAGGCAATCATGGCCAAATTAATATTAGCTCCTAAGATAAAACAATA ATATATAGTTAAAAAATTGTTAT CTTTTGTGCAGGCTATCCGCATCATTTCATCCATGTCCATACGGATTTTTTCTTTTTCGT ACAAATTATGTAGGTCAAACAGC TTATTATAGCAAAGAGCACATGTTAACCACCACGTATTCAGATACTTAAAATGTTGGTAA ACATAAGAAATGGCCTCCCTAAG ATTATCCTGCAATGCCACGATAAAACAGTATATAGTTAACATATCACCATCCGAAATATT ACTTAATACGTTGGTATCTTCTG CTAAATTTTTTAGCTTCCAATGTATACACGACTTTATTTCCCTTATAATGACATAGGCTG AAAAGGGATTGTCATTAAAAAAT TTAAGACATAAGATAATATTATTACTAGTAGTGTTAGGATGTATTAATTTAAAGAATATG TGCATAATCTTCTTTTTATCCAC TTGGTACTTGGCTCCTAATTCCCAGCAAAATTCTCGAATAGGTGGCGTATTCGCGCAAAT TAACCCATAGTTGATGTCTGCGC CCCAATCCGTAAACATTTTTATTAATTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTG CCGTATTAAGGTCCAAGCCATCT GCAAAGTTTGGCAGCTTTATCAGCATATGTTTGCAATCAAGTGAAATTGGAGCCTTATAC CACCATAGCCCGCAGCGTTCTAA GATAACATGGTAATCAATAGATACATGCTGACTGGCTAATACCTTTTTGGCGAAGGATTG CAAGGAAGGAACCAT

SEQ ID No. 68 - Benin 97/1 MGF 360 10L (NC_044956.1:19607-20677)

T TATAAC GAT G TAT CAT T GAT GT CATAAT T C AAAT AG G C CAACAT AC t T T T T GAT T TAT AG t T T T T T AATAGAC GAT AT AT T T TGTTAGGATCTGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATGTCTAATG ATAAACGATGAGATATCAAATAG TAATTCTGTTGCTCTGCCAAGGCTTTTGCCTCTTCAAAGGCATCGGCCCCCAGATCTATA CAAAAGAACAGGTTATCCATATT ATAGAATCGTATGGAGGCAATCATGGCCAAATTAATATTAGCTCCTAAGATAAAACAATA ATATATAGTTAAAAAATTGTTAT CTTTTGTGCAGGCTATCCGCATCATTTCATCCATGTCCATACGGATTTTTTCTTTTTCGT ACAAATTATGTAGGTCAAACAGC TTATTATAGCAAAGAGCACATGTTAACCACCACGTATTCAGATACTTAAAATGTTGGTAA ACATAAGAAATGGCCTCCCTAAG ATTATCCTGCAATGCCACGATAAAACAGTATATAGTTAACATATCACCATCCGAAATATT ACTTAATACGTTGGTATCTTCTG CTAAATTTTTTAGCTTCCAATGTATACACGACTTTATTTCCCTTATAATGACATAGGCTG AAAAGGGATTGTCATTAAAAAAT TTAAGACATAAGATAATATTATTACTAGTAGTGTTAGGATGTATTAATTTAAAGAATATG TGCATAATCTTCTTTTTATCCAC TTGGTACTTGGCTCCTAATTCCCAGCAAAATTCTCGAATAGGTGGCGTATTCGCGCAAAT TAACCCATAGTTGATGTCTGCGC CCCAATCCGTAAACATTTTTATTAATTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTG CCGTATTAAGGTCCAAGCCATCT GCAAAGTTTGGCAGCTTTATCAGCATATGTTTGCAATCAAGTGAAATTGGAGCCTTATAC CACCATAGCCCGCAGCGTTCTAA GATAACATGGTAATCAATAGATACATGCTGACTGGCTAATACCTTTTTGGCGAAGGATTG CAAGGAAGGAACCAT

SEQ ID No. 69 - Mkuzi 1979 MGF 360 10L (AY261362.1 :27069-28139)

T TATAAC GAT G TAT CAT T GAT GT CATAAT T C AAAT AG G C CAACAT AC t T T T T GAT T TAT AG t T T T T T AATAGAC GAT AT AT T T TGTTAGGATCTGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATGTCTAATG ATAAACGATGAGATATCAAATAG TAATTCTGTTGCTCTGCCAAGGCCTTTGCCTCTTCAAAGGCATCGGCCCCCAGATCTATA CAAAAGAACAGGTTATCCATATT ATAGAATCGTATGGAGGCAATCATGGCCAAATTAATATTAGCTCCTAAGATAAAACAATA ATATATAGTTAAAAAATTGTTAT CTTTTGTGCAGGCTATCCGCATCATTTCATCCATGTCCATACGGATTTTTTCTTTTTCGT ACAAATTATGTAGGTCAAACAGC TTATTATAGCAAAGAGCACATGTTAACCACCACGTATTCAAATACTTAAAATGTTGGTAA ACATAAGAAATGGCCTCCCTAAG ATTATCCTGCAATGCCACGATAAAACAGTATATAGTTAACATATCACCATCCGAAATATT ACTTAATACGTTGGTATCTTCTG CTAAATTTTTTAGCTTCCAATGTATACACGACTTTATTTCCCTTATAATGACATAGGCTG AAAAGGGATTGTCATTAAAAAAT TTAAGACATAAGATAATATTACTACTAGTAGTGCTAGGATGTATTAATTTAAAGAATATG TGCATAATCTTCTTTTTATCCAC TTGGTGCTTGGCTCCTAATTCCCAGCAAAATTCTCGAATAGGTGGCGTATTCGCGCAAAT TAACCCATAGTTGATGTCTGCGC CCCAATCCGTAAACATTTTTATTAATTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTG CCGTATTAAGGTCCAAGCCATCT GCAAAGTTTGGCAGCTTTATCAGCATATGTTTGCAATCAAGTGAAATTGGAGCCTTATAC CACCATAGCCCGCAGCGTTCTAA GATAACATGGTGATCAATAGATACATGCTGACTGGCTAATACCTTTTTGGCGAAGGATTG CAAGGAAGGAAACAT

SEQ ID No. 70 - Pretorisuskop/96/4 MGF 360 10L (AY261363.1:26116-27186)

T TATAAC GT T G TAT TAT T GAC GT CATAAT T C AAAT AG G C CAAT AT AC t T T T T GAT T TAT AG t T T T T T AATAGAT GAT AT AT T T TGTTAGGATCCGCTTCTTTTAACGTTAATAACGAGGAATCTGGACTATAAATGTCTAATG ATAAACAATGAGATATCAAAAAG TAATTCCGTTGCTCTGCCAGGGCTTTTGCCTCCTCAAAGGCATCGGCCCCCAGGTCTATA CAAAAGAATAAGTTATCTATGTT ATAGAATTGTATGGAGGCAATCATAGCTAAATTAATATTAGCTCCTAAGATAAAACAATA ATATATGGTTAAAAAATTGTTAT CTTTTGTGCAGGCTATCCGCATCATTTCATCCATGTCCATGCGGATCTTTTCCTTTTCAT ACAAATGATGTAGGTCAAACACC TTATTATAGCAAAGAACACATGTTAACCACCACGTATTCAGATACTTAAAATGTTGGTAA ACATAAGAAATGGCCTCCCTAAG ATTATCCTGCAATGCCACGATAAAACAGTATATAGTTAACATATCTCCATCCGAAATATT ACTTAATACGTTGGTATCTTCTG CTAACTTTTTTAGCTTCCAATGTATACACGACTTTATTTCCCTTATAATGACATAGGCTG AAAAGGGATTGTCATTAAAAAGT TTAAGACATAAGATAATATTACTACTAGTAGTGCTAGGATGTATTAATTTAAAGAATATG TGCATAATCTTCTTTTTATCCAC TTGGTACTTGGCTCCTAATTCCCAGCAAAATTCTCGAACAGGTGGCGTATTCGCGCAAAT TAACCCATAGTTGATGTCTGCGC CCCAATCCGTAAACATTTTTATTAATTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTG CCGTATTAAGGTCCAAGCCATCT GCAAAGTTTGGCAGCTTTATCAGCATATGTTTGCAATCAAGTGAAATTGGAGCCTTATAC CACCATAGCCCGCAGCGTTCTAA

GATAATATGGTGATCAATGGATACATGCTGTCTGGCTAGTACCTTTTTGGCGAAGGA TTGTAAGGAAGGAAACAT

SEQ ID No. 71 - Tengani 62 MGF 360 10L (AY261364.1 :20530-21600)

T TATAACAAT G TAT CAT T GAT AT CAT CAT T CAAAT AG G C CAACAT AT t T T T T GAT T TAT AG t T T T T T AATAGAT GAT T TAT T T TGTTAGGATCTGTTTCTTTTAACGTTAATAGCAAGGAGTCTGGCTTATAAATGTCTAATG ATAAACGATGAGATATTAAATAG TAATTCCGTTGCTCTGCCAGGGCTTTTGCCTCTTCAAAGGCGTCGGCTCCCAGATCTATA CAAAAGAACAAGTTATCCATATT ATAAAATCGTACGCAGGCAAGCATAGCTGAATTAATATTAGCTCCTAAGAGAAAACAATA ATATATAGTTAAAAAATTGTTAT CTTTTGTGCAGGCCATCCGCATCATTTCATCCACGTCCATGCGGATCTTTTCCTTTTCAT ACAAATTATGTAGGTCAAACAGC TTATTAAAACAAAGAGCACAGATTAACCACCACGTATTTAGATACTTAAAATGTTGGTAA ACATAAGAAATGGCCTCCCTAAG ATTATCCTGCAATGCCACTATAAAACAGTATATCATTAACATATCACCATCCGACATATT ACTTAATATGTCGGTGTCTTCTA CTAACCTTTTCAGCTTCCAATATATGGATATCCTTATTTCCCTTATAATGACATAGGCTG GAAAGGGATTATCATTAAAAAGT TTAAGACATAAGATAATATTACTGCTAGTAGTGCCAGGATGTATTAATTTAAAGAACATG TGTATAATCTTCTTTTTATCCAC GCGGTGCTTGGCTCCTAATTCCCAGCAAAATTCTCGAACAGACGGCGTATTGGCGCAAAT TAACCCATAGTTGATGTCTGCGC CCCATTCCGTAAACAGTTTTATTAACTGATAGTTATTTTCCTTTGTAGCCAACATTAGTG CCGTATTAAGGTCCAAGCCGTCT GCAAAGCTTGGCAGCTTTATCAGCATATGTTTGCAGTCAAGTGAAATTGGAGCCTTATAC CACCATAGTCCGCAACGTTCTAA GATAACATGGTACTCAATAGATACTTGCTGTCTGGCTAGTACCTTTTTGGCGAAGGATTG TAAGGAAGGAAACAT

SEQ ID No. 72 - Warthog MGF 360 10L (AY261366.1 :22069-23130)

TCATATTGTATCATCATGGTTAAAATATGCCGTCATATTTTTTGATTTATAGTTTTT TAATAGATGATATATTTTGTTAGGGT

CCGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATGTCTAATGATAAAC GATGAGATATCAAAAAGTAATTCCGT

TGCTCTGCCAGGGCTTTTGCCTCTTCAAAGGCATCGGCCCCCAAGTCTATACAAAAG AACAGGTTATCCATATTATAAAATCG

TATGGAGGCAATCATGGCCAAATTAATATTAGCTCCTAAGATAAAACAATAATATAT AGTTAAAAAATTGTTATCTTTTGTGC

AGGCTATCCGCATCATTTCATCCATGTCCATGCGGATTTTTTCCTTTTCGTACAAAT TATGTAGGTCAAACAGCTTATTAAAA

CAAAGAGCACAGATTAGCCACCACGTATTCAGATATTTAAAATGTTGGTAAACATAA GAAATGGCCTCCCTAAGATTATCCTG

CAAT G C CAC CATAAAACAG TATAT C GT TAACAT AT CAC CAT C C GAAATAT TACT T AATAC G TCGGTGTCTTCTAC TAAC T T T T

T CAG C T T C CAATATAT G GAT GAC T T TAT T T C C C T T AT AAT GACAT AG G C T G AAAAG G G G T TAT CAT T AAAAAG T T TAAGACAT

AAGATAATATTACTGCTAGTAGTGCCAGGATGTATTAATTTAAAGAACATGTGCATA ATCTTCTTTTTATCCACGCGGTACTT

GGCTCCTAGTTCCCAGCACAATTCTCGAACAGGCGGCGTATTGGCGCAAATTAACCC ATAGTTGATGTCTGCGCCCCATTCCG

TAAACAGTTTTATTAACTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTGCCGTAT TAAGGTCCAAGCCATCTGCAAAGCTT

GGCAGCTTTATCAGCATATGTTTGCAATCAAGGGAAATTGGAGCCTTATACCACCAT AGTCCGCAGCGTTCTAAGATAACATG

GTAATCAATAGATACATGCTGTCTGGCTAGTACCTTTTTGGCGAAGGATTGTAA

G GAAG GAAACAT

SEQ ID No. 73 - Warmbaths MGF 360 10L (AY261365.1 :25300-26361)

TCATATTGTATCATCATGGTTAAAATATGCCGTCATATTTTTTGATTTATAGTTTTT TAATAGATGATATATTTTGTTAGGGT

CCGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATGTCTAATGATAAAC GATGAGATATCAAAAAGTAATTCCGT

TGCTCTGCCAGAGCCTTTGCCTCTTCAAAGGCGTCGGCCCCCAGGTCTATACAAAAG AACAGGTTATCCATATTATAGAATCG

TATGGAGGCAATCATGGCCAAATTAATATTAGCTCCTAAGATAAAACAATAATATAT AGTTAAAAAATTGTTATCTTTTGTGC

AGGCTATCCGCATCATTTCATCCATGTCCATGCGGATTTTTTCCTTTTCGTACAAAT TATGTAGGTCAAACAGCTTATTAAAA

CAAAGAGCACAGATTAGCCACCACATATTCAGATACTTAAAATGTTGGTAAACATAA GAAATGGCCTCCCTAAGATTATCCTG

CAATGCCACCATAAAACAGTATATCGTTAACATATCACCATCTGAAATATCACTTAA TACGTCGGTGTCTTCTACTAACTTTC

T CAG C T T C CAATATAT G GAT GAC T T TAT T T C C C T TAT AAT GACAT AG G C T G AAAAG G G G T TAT CAT T AAAAAG T T TAAGACAT

AAGATAATATTACTGCTAGTAGTGCCAGGATGTATTAATTTAAAGAACATGTGCATA ATCTTCTTTTTATCCACGCGGTACTT

GGCTCCTAGTTCCCAGCACAATTCTCGAACAGGCGGTGTATTGGCGCAAATTAACCC ATAGTTGATGTCTGCGCCCCATTCCG

TAAACAGTTTTATTAACTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTGCCGTAT TAAGGTCCAAGCCATCTGCAAAGCTT

GGCAGCTTTATCAGCATATGTTTGCAATCAAGTGAAATTGGAGCCTTATACCACCAT AGTCCGCAGCGTTCTAAGATAACATG

GTAATCAATAGATACATGCTGTCTGGCTAGTACCTTTTTGGCGAAGGATTGTAA

G GAAG GAAACAT

SEQ ID No. 74 - Malawi Lil-20/1 (1983) MGF 360 10L (AY261361.1 : 23735-24802)

TTACACTGTGTCATGCCCATCATAGTCAAAATATACCATCATATTTTTTGATTTATA GTTTTTTAATAGATGATATATTTTTT

TAGGATCTGCTTCTTTTAACGTTAATAGCGAGGAGTCTGGACTATAAATATCTATTG ATAAACGATGATACATCAAAAAGTAA

TTCCGCTGGTCTGCCAGGGCTTTTGCCTCTTCAAAGGCATCGGCTCCCAGGTCTATA CAAAAGAACAAGTTATCCATATTATA

GAATTGTACGCAGGCAAGCATAGCCTGATTAATATTAGCTCCTAAGAGAAAACAGTA ATATATGGTTAAAAAGTTGTTATCTT

TAGTGCAGGCTATGCACATCATTTCATCCATGTCCATGCGGATCTTTTCCTTTTCAT ACAAATCATGTAGGTCAAACAGCTTA

TTAAAACAAAGAGCACAGATTAACCACCACGTATTCAGATGCTTAAAATGTTGGTAA AAATAAGAAATGGCCTCCCTAAGATT

ATCCTGCAATGCCAGGATAAAACAGTATATAGTCAACATATCATCATCCGACATATT ACTTAATATGTCAGTGTCTTCTACTA

ACCTTCTCAGCTTCCAATATATAGACGACTTTATTTCCCTTATAATGACATAGGTTG GAAAAGGATTATTATTAAAAAGTTTA

AGACATAAGATAATATTACTACTAGTAGTGCCATGATGTATTAATTTAAAAAACATA TGCATAACCTTATTTTTATCCACTTG

GTACTTGGCTCCTAATTCCCAGCAAAATTCTCGAACAGGCGGCGTATTAGCGCAAAT TAATCCATAGTTGATGTCCGCGCCCC ATTCCGTAAACAGTTTTATTAACTGATAGTTGTTTTCCTTTGTAGCCAACATTAGTGCCG TATTAAGGTCCAAGCCATCTGCA

AAGCTTGGCAGCTTTATTAACATATGTTTGCAATCAAGTGAAATTGGAGCCTTATAC CACCATAGTCCGCAATGTTTTAAGAT

ATAATGATAATCAATGGATACATGATGTCTAGCTAATACCTTTTTGGCGAAGGATTG TAAAGAAGGAAACAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 10L. Suitably the functional version of MGF 360 10L comprises the sequence of SEQ ID No. 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73 or 74. Suitably the functional version of MGF 360 10L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73 or 74. Suitably the functional version of MGF 360 10L consists of the sequence of SEQ ID No. 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73 or 74.

MGF 36011 L gene sequences

SEQ ID No. 75 - Georgia 2007/1 MGF 360 11L (NC_044959.1 :26465-27526)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCAATTTATTTTTTCAGGGTCCG TAACGATCGACAACAGAGAAATAACCGGATTGTAATGCTTTAATGATAAGGCATGGGCTA TCAGATAATTTTCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCATCGGCACCCAGGTCTATACAAAAGAACAGGTTT CCAAGATTATAGTTTTGTATGGA AACAAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCCGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATTTCATCAATATCCATGCGGACCCTTTCTATTTCGTACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGACGCGGGTATTTCTGGTAAAAGTAGCGGATAGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGCTACTGGTTTTATCGCGCGTTGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAACAGGTTGAC CTTATAAATAATTTATTGCGTCGAATATGAAGCATAATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGACATGATA TTGATCTACCGGTATACACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT

SEQ ID No. 76 - China/2018/AnhuiXCGQ MGF 360 11L (MK128995.1:26467-27528)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCAATTTATTTTTTCAGGGTCCG TAACGATCGACAACAGAGAAATAACCGGATTGTAATGCTTTAATGATAAGGCATGGGCTA TCAGATAATTTTCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCATCGGCACCCAGGTCTATACAAAAGAACAGGTTT CCAAGATTATAGTTTTGTATGGA AACAAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCCGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATTTCATCAATATCCATGCGGACCCTTTCTATTTCGTACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGACGCGGGTATTTCTGGTAAAAGTAGCGGATAGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGCTACTGGTTTTATCGCGCGTTGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAACAGGTTGAC CTTATAAATAATTTATTGCGTCGAATATGAAGCATAATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGACATGATA TTGATCTACCGGTATACACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT SEQ ID No. 77 - L60 MGF 360 11L (NC_044941.1:21052-22113)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGTAATTTATTTTTTCAGGGTCCA TAACGTTCGACAACAGAGAAATGACTGGATTGTAATGCTTTAATGATAAGGCATGGGCTA TCAGATAATTTTCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCGTTGGCGCCCAGGTCTATACAAAAGAACAGGTTT CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCCGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATTTCATCGATATCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCGATGTAGGCGCGGGTATTTCTGGTAAAAGTAGCGAATAGCA TCTTTGAGGTCATAGTCCACCGC TATAGCATACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCTAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGCTACTGGTTTTATCGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATAGTTTATTACGTCGAATATGAAGCATAATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA

ATGATCCACCGACACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGA AGGCAACAT

SEQ ID No. 78 - Benin 97/1 MGF 360 11L (NC_044956.1:20703-21764)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGTAATTTATTTTTTCAGGGTCCA TAACGTTCGACAACAGAGAAATGACTGGATTGTAATGCTTTAATGATAAGGCATGGGCTA TCAGATAATTTTCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCGTTGGCGCCCAGGTCTATACAAAAGAACAGGTTT CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCCGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATTTCATCGATATCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCGATGTAGGCGCGGGTATTTCTGGTAAAAGTAGCGAATAGCA TCTTTGAGGTCATAGTCCACCGC TATAGCATACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCTAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGCTACTGGTTTTATCGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATAGTTTATTACGTCGAATATGAAGCATAATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA ATGATCCACCGACACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT

SEQ ID No. 79 - Mkuzi 1979 MGF 360 11L (AY261362.1 :28164-29225)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGTAATTTATTTTTTCAGGGTCCA TAACGATCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATGGGCTA TCAGATAATTTCCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCGTTGGCGCCCAGGTCTATACAAAAGAACAGGTTT CCAAGATTATAGTTTTGTATGGA AACCAGCATAGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGACCGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATTTCATCAATATCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGGCGCGGGTATTTCTGGTAAAAGTAGCGAATAGCA TCTTTGAGGTCATAGTCCACCGC TATAGCATACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCTAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCTCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGCTACTGGTTTTATCGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATAGTTTATTACGTCGAATATGAAGCATAATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA ATGATCCACCGACACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT

SEQ ID No. 80 - Warmbaths MGF 360 11L (AY261365.1 :26388-27449)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCGATTTATTTTTTCAGGGTCCG TAACCACCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATTGGCTA TCAGATAATTTCCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCGTTGGCACCCAGGTCTATACAAAAGAACAGGTTC CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAGATGGCTGAATA GCTATAATCTTGGACGCAGGCTA TGTGCATCATTTCATCAATGTCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGCCGCGGGTATTTCTGGTAAAAGTAGCGGATAGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGTTACTGGTTTTATCGCGCGTTGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATATTCTATTACGTCGAATATGAAGCATTATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA ATGATCCACCGACACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT SEQ ID No. 81 - Pretorisuskop/96/4 MGF 360 11 L (AY261363.1:27214-28275)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCGATTTATTTTTTCAGGGTCCG TAACCACCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATTGGCTA TCAGATAATTTCCCTTTTGTTCT GCCAAAGCTTTGCCCTCTTCAAAGGCGTTGGCACCCAGGTCTATACAAAAGAACAGGTTC CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCTGAATA GCTATAATCTTGGACGCAGGCTA TGTGCATCATTTCATCAATGTCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGCCGCGGGTATTTCTGGTAAAAGTAGCGGATAGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGTTACTGGTTTTATCGCGCGTTGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATATTCTATTACGTCGAATATGAAGCATTATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA

ATGATCTACCGACACGCACTGCCCAGCCAGTACTTTCTTCGTGAGGGATTGCAGGGA AGGCAACAT

SEQ ID No. 82 - Tengani 62 MGF 360 11L (AY261364.1 :21627-22688)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCAATTTATTTTTTTAGGGTCTG TAACGACCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATCGGCTA TCAGATAATTTCCCTTTTGTTCT GCCAAAGCTTTGCCTTCCTCAAAGGCGTTGGCTCCCAGGTCTATACAAAAGAACAGGTTC CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCCGAATA GCTATAATCTTGGACGCAGGCTA TGTGCATCATTTCATCAATGTCCATGCGGACCCTTTCTATTTCGTACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGG GCGCACATGAGCCGCCACCTATGTAGACGCGGGTATTTCTGGTAAAAGTAGCGGATCGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTGCTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCCCCCCTCAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGTTACTGGTTTTATCGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCACC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATAGTTTATTACGTCGAATCTGAAGCATAATGGGTCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA ATGATCCACCGACACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT

SEQ ID No. 83 - Warthog MGF 360 11L (AY261366.1 :23157-24218)

TTATCTTTGTTCATAATCAAGAAAAATCCCCATATTTATTGAGTGATAATTTTTTAA CATGCGATTTATTTTTTCAGGGTCCG TAACCACCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATTGGCTA TCAGATAATTTCCCTTTTGTTCT GCCAAAGCTTTGCCCTCCTCAAAGGCGTTGGCCCCCAGGTCTATACAAAAGAACAGGTTC CCAAGATTATAGTTTTGTATGGA AACCAGCATGGCTTGATTGATGTTGGCTCCCATGATAAAACAGTAGTAAATGGCTGAATA GCTATAATCTTGGACGCAGGCTA TGTGCATCATTTCATCAATGTCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACGTTGTTGTAAAAAAGA GCGCACATGAGCCGCCACCTATGTAGCCGCGGGTATTTCTGGTAAAAGTAGCGGATAGCA TCTTTGAGGTCATAGTCCACCGC TATCGCGTACCAGTATTTGGTTAAAACAGTACTAAAGCTATCATCATGGTCCAGCATGAA GGTTATCTCCATGAGCCCTCTTA ACTCCCACATGATTTCTCCCCTTAGATCCAGATTATCTATAATCCTTAAATTGGGGTTAT TGGAAAACACCTCGTGGCAAAAG ATAATATTGTTACTGGTTTTATCGCGCGTTGTATCAAAGAAAATTTTTAAAATATACTCT CTTTCTAAATATTCTTTGGCTCC CAGCTCTTTGCACAGATCACGGGTATTTTCCGTGAGAGCACAAATCATTCCATAGTTAAT ATCTGCACCCCATTCAGTAAACA GCTTTATCAAGTCATGATTATTCTCCTTCACGGCTTTCATCAGTCCTATGTTTAACTCGA TACCTTGACTAAAGCAGGTTGAC CTTATAAATATTCTATTACGTCGAATATGAAGCATTATGGGGCCATTATGCCACCACAGG CCACAACACTTCAGGATATGATA ATGATCCACCGATACGCACTGCCCGGCCAGTACTTTCTTCGTGAGGGATTGCAGGGAAGG CAACAT

SEQ ID No. 84 - Malawi Lil-20/1 (1983) MGF 360 11L (AY261361.1 :24831-25892)

TTATCTTCGTTCATAATCAAGAAAAACCCTCATATTTATTGAGTGATAATTTTTTAA CATGCAATTTATTTTTTCAGGGTCCA TAATGACCGACAACAGAGAAATGACCGGATTGTAATGCTTTAATGATAAGGCATCGGCTA TCAGATAATTTTCCTTTTGTTCT GCCAAAGCTTTCCCCTCCTCAAAGGCGTTGGCCCCCAGATCTATACAAAAGAACATGTTC CCAAGATTATAGTTTTGTATAGA AAC AAG CAT G G CT T GAT T GAT GTTGGCCCC CAT GATAAAACAG TAAT AAAT AG C C G CAT AG CT AT AAT C T T G GAC G CAG G C TA

TGTGCATCATTTCATCAATATCCATGCGGACCCTTTCTATTACATACAGCTCGTGAA GGTCGAACACGTTGTTGTAAAAAAGG GCGCACATAAGCCGCCAGTGGTGTAGATGGGTATACCTCTGATAAAAATAATGGATAGCC TTTTTGAGGCCATAGTCTACCGC TATGGCGTACCAGTATTTGGTTAACATATTGCTAAAGGAGTTGTCATGGTCCAACATGTA GGTTATCTCCATGAGTCCCCTTA GCTCCCACATGATTTCCTCCCTCAGATCCAAATTATCCACATTTTTCAGGTTGGGGTTAT TGGAAAACATTTCGTGGCATAAG ATAATATTACTGTCGGTTTTGTTGCGCGTCGTATCAAAGAAAATTTTTAAAATATAATCT CTATCTAAACGATCTTTGGCTCC TAGTTCTCTGCATAGATTACGGGTGTGCTCCGTATGGGCACAAATCAGTCCATAATTAAT ATCGGCACCCCACTCGGCAAACA GCCTTATCAAGTCATGGTTGTTTTCCTTCGCGGCTTTCATCAGTACGGTGTTCAATTCAA TACCCTCGCTAAAACAGGTTGAC CTTATAAACATTTTGTGACGATAAACGTAAAATGTAATAGGGCCATCATACCACCACAAC CCGCAGCACTTTAAGACATGATA TTGATCTACCGGTACACACTGTTTGGCCAGCACTTTCTTAGTTAAAGATTGTAAGGAAGG CAACAT SEQ ID No. 85 - Kenya 1950 MGF 360 11L (AY261360.1 :30655-31716)

TTATCTGCGTTCATAATCGAGAAAAACCACCATATTCTTTGAGTGATAATTTTTTAA CATAGAATTTATCTTTTCTGGGTCGG TCACGGCCGACAACAGGGAAATCACCGGGTTATAATGTTTTAATGATAAGGCATCGGCTA TCAAATAATTTTCCTTTTGTATT GCTAGGGTTTTGCCTTCTTCAAAGGCGTTGGCACCCAGGTCTATACAAAAGAACATGTTC CCGAGATTATAGTTTTGTATGGA AACAAACATGGCTTGATTGATGTTGGCCCCCATGATAAAACAGTAGTAGATAGCTGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATCTCATCGATATCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACATTGTTATAAAAAAGG GCGCACATAAGCCGCCACCGATGTAGATGGGTATACTTCTGATAAAAATAGTGGATGGCC TTTTTGAGGCCGTAGTCTACCGC TATGGCGTACCAGTATTTGGTTAACATATTGCTGAAGGAGTTGTCATGGTCCAACATGTA GGTTATCTCCATGAGTCCCCTTA GCTCCCACATGATTTCCTCCCTCAGATCCAAATCATCCACATGTTTCAGATTGGGGTTAT TGGAAAACATTTCGTGGCATAAG ATAATATTACTGTCGGTTTTGTTGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTATCTAAACGGTCTTTGGCTCC AAGTTCTCTGCATAGATTGCGGGTGTGCTCCGAATGAGCACAAACCAGTCCATAATTAAT ATCGGCACCCCACTCAACAAACA GCCTTATCAAGTCATGATTGTTTTCCTTCGCGGCTTTCATCAGTACGGTGTTCAATTCAA TACCCTCGCTAAAACAGGTTGAC TTTATAAACATTTTGTTACGATACGTATAAAATGTAATAGGGCCATCATACCACCACAAC CCGCAGCATTTCAGGATATGATA

TTGATCTACCGGTATACACTGTTTGGCCAGCACTTTCTTGGATAGGGATTGCAAGGA AGGCAACAT

SEQ ID No. 86 - Ken05/Tk1 MGF 360 11 L (NC_044945.1 :28407-29468)

TTATCTGCGTTCATAATCGAGAAAAACCACCATATTCTTTGAGTGATAATTTTTTAA CATAGAATTTATCTTTTCTGGGTCGG TCACGGCCGACAACAGGGAAATCACCGGGTTATAATGTTTTAATGATAAGGCATCGGCTA TCAAATAATTTTCCTTTTGTATT GCTAGGGCTTTGCCTTCTTCAAAGGCGTTGGCACCCAGGTCTATACAAAAGAACATGTTC CCGAGATTATAGTTTTGTATGGA AACAAACATGGCTTGATTGATGTTGGCCCCCATGATAAAACAGTAGTAAATAGCTGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATCTCATCGATATCCATGCGGACCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACATTGTTATAAAAAAGG GCGCACATAAGCCGCCACCGATGTAGATGGGTATACTTCTGATAAAAATAGTGGATGGCC TTTTTGAGGCCGTAGTCTACCGC TATGGCGTACCAGTATTTGGTTAACATATTGCTGAAGGAGTTGTCATGGTCCAACATGTA GGTTATTTCCATGAGTCCCCTTA GCTCCCACATGATTTCCTCCCTCAGATCCAAATCATCCACATGTTTCAGATTGGCGTTAT TGGAAAACATTTCGTGGCATAAG ATAATATTACTGTCGGTTTTGTTGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT CTATCTAAACGGTCTTTGGCTCC AAGTTCTCTACATAGATTGCGGGTGTGCTCCGAATGAGCACAAACCAGTCCATAATTAAT ATCGGCACCCCACTCAACAAACA GCCTTATCAAGTCATGATTGTTTTCCTTCGCGGCTTTCATCAGTACGGTGTTCAATTCAA TACCCTCGCTAAAACAGGTTGAC TTTATAAACATTTTGTTACGATACGTATAAAATGTAATAGGGCCATCATACCACCACAAC CCGCAGCATTTCAGGATATGATA TTGATCTACCGGTATACACTGTTTGGCCAGCACTTTCTTGGATAGGGATTGCAAGGAAGG CAACAT

SEQ ID No. 87 - Ken06.Bus MGF 360 11L (NC_044946.1:24534-25595)

TTATCTGCGTTCATAATCGAGAAAAACCACCATATTCTTTGAGTGATAATTTTTTAA CATAGAATTTATCTTTTCCGGGTCGG TCACGGCCGACAACAGGGAAATCACCGGGTTATAATGTTTTAATGATAAGGCATCGGCTA TCAAATAATTTTCCTTTTGTATT GCTAGGGCTTTGCCTTCTTCAAAGGCGTTGGCACCCAGGTCTATACAAAAGAACATGTTC CCGAGATTATAGTTTTGTATGGA AACAAACATGGCTTGATTGATGTTGGCACCCATGATAAAACAGTAGTAAATAGTTGAATA GCTATAATCTTGGATGCAGGCTA TGTGCATCATCTCATCGATATCCATGCGGATCCTTTCTATTTCATACAGCTCGTGAAGGT CGAACACATTGTTATAAAAAAGG GCGCACATGAGCCGCCACCGATGTAGATGGGTATACTTCTGATAAAAATAGTGGATGGCC TTTTTGAGGCCGTAGTCTACCGC TATGGCGTACCAGTATTTGGTTAACATGTTGCTGAAGGAGTTGTCATGGTCCAACATGTA GGTTATCTCCATGAGTCCCCTTA GCTCCCACATGATTTCCTCCCTCAGGTCCAAATCATCCACATGTTTCAGATTGGGGTTAT TGGAAAACATTTCGTGGCATAAG ATAATATTACTGTCGGTTTTGTTGCGCGTCGTATCAAAGAAAATTTTTAAAATATACTCT TTATCTAAACGGTCTTTGGCCCC TAGTTCTCTGCATAGATCGCGGGTGTGCTCCGAATGAGCACAAACCAGTCCATAATTAAT ATCGGCACCCCACTCAGTAAATA ATTTTATTAGGTCATGATTGTTTTCCTTCGCGGCTTTCATCAGTACGGTGTTCAATTCAA TACCCTCGCTAAAACAGGTTGAC TTTATAAACATTTTGTTACGATACGTATAAAATGTAATAGGGCCATCATACCACCACAAC CCGCAACTTTTCAGGATATGATA TTGATCTACCGGTATACACTGTTTGGCCAGCACTTTTTTGGATAGGGATTGCAAGGAAGG CAACAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 11 L. Suitably the functional version of MGF 360 11 L comprises the sequence of SEQ ID No. 75, 76, 77, 78, 79, 80, 81 , 82, 83, 84, 85, 86 or 87. Suitably the functional version of MGF 360 11 L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86 or

87. Suitably the functional version of MGF 360 11 L consists of the sequence of SEQ ID No. 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86 or 87.

MGF 36012L gene sequences

SEQ ID No. 88 - Georgia 2007/1 MGF 360 12L (LR743116.1 :30355-31407) TCATCTTAAATCATAGGTAAGGAAGATCATCATATTTTTTGAAACGTAATTTTTTAACGC ATGATCTATGATTTCAGGGTCCG TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCTTTA TAAGCTCATACCCTTCCCCTAAA GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCTATACAAAAGAACATGTTTTCT ATATTATAGTACTGTATTGAGCT AAGCATGGCTTGATTGATGTTGGCGCCCAGGACATAGCAGTAGTACATGGTTGAAAGGTT GTGGTCTTTGATGCAGGCGATCC GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGAAGGTCAA ACACATTATTAAAACAAAGAGCA CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCCTCT TTCAGGTTATAGCGTATGGCTAT AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCGAAAACTCATTATTTAGCAGATCGGT TTTTACTATTAACTCCCTTAACT CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGTTGG AAAACACCTCATGGCATAAAATA ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCTTCA TTCAAGGTTTCCTTGGCACCTAG CTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATACCAGCCCGTAGTTGATGTC CGCCCCCCACTCTGCAAACAGTT TTATAAGGTTGTAGTTGTTTTCCCTTACAGCCTTCACTAACGCCGTATTTAGGTTTAAGC CCTCTTTAATACCTGCTGATTTT ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATCATGCCACCATAGGTCATAACAC TTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 89 - China/2018/AnhuiXCGQ MGF 360 12L (MK128995.1:29384-30436)

TCATCTTAAATCATAGGTAAGGAAGATCATCATATTTTTTGAAACGTAATTTTTTAA CGCATGATCTATGATTTCAGGGTCCG

TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCT TTATAAGCTCATACCCTTCCCCTAAA

GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCTATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGCT

AAGCATGGCTTGATTGATGTTGGCGCCCAGGACATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCGATCC

GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGAAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGTTATAGCGTATGGCTAT

AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTACTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCT TCATTCAAGGTTTCCTTGGCACCTAG

CTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATACCAGCCCGTAGTTGAT GTCCGCCCCCCACTCTGCAAACAGTT

TTATAAGGTTGTAGTTGTTTTCCCTTACAGCCTTCACTAACGCCGTATTTAGGTTTA AGCCCTCTTTAATACCTGCTGATTTT

ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATCATGCCACCATAGGTCATAA CACTTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 90 - Tengani 62 MGF 360 12L (AY261364.1 :24540-25592)

T CAT C T T AAAT CATAG G TAAG GAAGAT CAT CAT AT t T T T T G AAAC GT AAT T T T T T AAC G CAT GAT CT AT GAT T T CAG G G T C CA TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCTTTA TAAGCTCATACCCTTCCCCTAAA GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCTATACAAAAGAACATGTTTTCT ATATTATAGTACTGTATTGAGCT AAGCATGGCCTGATTGATGTTGGCGCCCAGGACATAGCAGTAGTACATGGTTGAAAGGTT GTGGTCTTTGATGCAGGCGATCC GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGAAGGTCAA ACACATTATTAAAACAAAGAGCA CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCCTCT TTCAGGTTATAGCGTATGGCTAT AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCGAAAACTCATTATTTAGCAGATCGGT TTTTTCTATTAACTCCCTTAACT CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGTTGG AAAACACCTCATGGCATAAAATA ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCTTCA TTCAAGGTCTCCTTGGCACCTAG CTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATACCAGCCCGTAGTTGATGTC CGCCCCCCACTCTGCAAACAGTT TTATAAGGTTGTAGTTGTTTTCCCTTACAGCCTTTACTAACGCCGTATTTAGGTTTAAGC CCTCTTTAATACCTGCTGATTTT ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATCATGCCACCATAGGTCATAACAC TTTAAAAGATAATGTTGGTTCGT GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 91 - Warthog MGF 360 12L (AY261366.1 :26036-27088)

TCATCTTAAATCGTAGGTAAGGAAGATCATCATATTTTTTGAAACGTAATTTTTTAA CGCATGATCTATAATTTCAGGGTCCG

TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCT TTATAAGCTTATACCCTTCCCCTAAA

GCTATAGTGCCCTCTTCGAAAGCATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGGCGCCCAGGACATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCGATCC

GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGAAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGTTATAGCGTATGGCTAT

AGCGTACCAGTATTTGAGTAGTAATGCACTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCT TCATTCAAGGTCTCCTTGGCACCTAG

CTCTCGACAGAGGTCCCGGGTGTGCTCCGTGTTGACAGATACCAGCCCATAGTTGAT GTTCGCCCCCCACTCTGCAAACAGTT

TTATAAGGTTGTAATTGTTTTCCCTTACAGCCTTCACTAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGCTGATTTT

ATGAGCTTTAGGTTATGATCAAACGTGATCGGACCATCATGCCACCATAGGTCATAA CACTTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 92 - Warmbaths MGF 360 12L (AY261365.1 :29294-30346)

TCATCTTAAATCGTAGGTAAGGAAGATCATCATATTTTTTGAAACGTAATTTTTTAC CGCATGATCTATGATTTCAGGGTCCG

TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCT TTATAAGCTTATACCCTTCCCCTAAA

GCTATAGTACCCTCTTCGAAAGCATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTACATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGGCGCCCAGAACATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCGATCC

GCATCATCTCTTCTATGTCCATATAGATCTTGTCCTTTTCATACGCCTCATGAAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCATGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGTTATAGCGTATGGCTAT

AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCT TCATTCAAGGTCTCCTTGGCACCTAG

CTCTCGACAGAGGTCCCGGGTGTGCTCCGTGTTGACAGATACCAGCCCATAGTTGAT GTCCGCCCCCCACTCTGCAAACAGTT

TTATAAGGTTGTAGTTGTTTTCCCTTACAGCCTTCACTAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGCTGATTTT ATGAGCTTTAGGTTATGATCAAACGTGATCGGACCATCATGCCACCATAGGTCATAACAC TTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 93 - Pretorisuskop/96/4 MGF 360 12L (AY261363.1:30098-31150)

TCATCTTAAATCGTAGGTAAGGAAGATCATCATATTTTTTGAAACGTAATTTTTTAA CGCATGATCTATAATTTCAGGGTCCG

TGCTTTTAGGCAACGGGGTGGTGGCCGGACTATAAATCTTTAGGGATAAAATGTTCT TTATAAGCTTATACCCTTCCCCTAAA

GCTATAGTGCCCTCTTCGAAAGCATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGCCCCCCAGGACATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCGATCC

GCATCATCTCTTCTATGTCCATATGAATCTTGTCCTTTTCATACGCCTCATGAAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGTTATAGCGTATGGCTAT

AGCGTACCAGTATTTAAGTAGTAATGTACTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTCT TCATTCAAGGTCTCCTTGGCACCTAG

CTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATACCAGCCCATAGTTGAT GTTCGCCCCCCACTCTGCAAACAGTT

TTATAAGGTTGTAGTTGTTTTCCCTTACAGCCTTCACTAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGCTGATTTT

ATGAGCTTTAGGTTATGATCAAACGTGATCGGACCATCATGCCACCATAGGTCGTAA CACTTTAAAAGATAATGTTGGTTTGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 94 - Mkuzi 1979 MGF 360 12L (AY261362.1 :31073-32125)

TCATCTTAAATCATAGGAAAGGAAGATCATCATATTTTTTGAAAAGTAATTTTTTAA CGCATGATCTATGATTTCAGGGTCCG

TGCTTTTAGGCAACGGGATGGTGGTCGGACTATAAATCTTTAGAGATAAAATGTTTT TTATAAGCTCATACCCTTCCCCTAAA

GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGGCGCCCAACATATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTAATGCAGGCGATCC

GCATCATCTCTTCTATGTCCATATAGATCTTGTCCTTTTCATACGCCTCATGGAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCT TCTTTCAGGTTATAGCGTATGGCTAT

AGCGTACCAGTATTTGAGTAGCAATGTACTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAAAAAATTTCTATCCTCATTTTTATATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATAAAAATTTGTAAAATTTCTTTT TCATTCAAGGTCTCCTTGGCACCTAG

CTCTCGACAGAGGTCCCAGGTGTGCTCCGAGCTGACAGATACCAGCCCATAGTTGAT GTCCGCCCCCCACTCTGCAAACAATT

TTATAAGGTTGTAGTTGTTTTCCTTTACAGCCTTCACCAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGCTGATTTT

ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATCATGCCACCATAGGTCATAA TATTTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCGGCCAACACCTTTTTGGTCAAAGATTGCAGGGAAGGCAACAT

SEQ ID No. 95 - L60 MGF 360 12L (NC_044941.1:23965-25017)

TCATCTTAAATCATAGGAAAGGAAGATCATCATATTTTTTGAAAAGTAATTTTTTAA CGCATGATCTATGATTTCAGGGTCCG

TGCTTTTAGGCAACGGGATGGTGGTCGGACTATAAATCTTTAGAGATAAAATGTTTT TTATAAGCTCATACCCTTCCCCTAAA

GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGGCGCCCAACATATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCAATCC

GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGGAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGCTATAGCGTACGGCTAT

AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCAAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCAATTTCAGATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATGAAAATTTGTAAAATTTCCTCT TCATTCAAGATCTCCTTGGCACCTAA

CTCTTGACAGAGGTCCCGGGTATGCTCCGTGTTGACAGATACCAGCCCATAGTTGAT GTCCGCCCCCCACTCTGTAAATAGTT

TTATAAGGCTGTAGTTGTTTTCCCTTACAGCCTTTACTAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGATGATTTT

ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATTATACCACCATAGATCATAA TATTTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAAAGATTGCAGGGAAGGCAACAT

SEQ ID No. 96 - Benin 97/1 MGF 360 12L (NC_044956.1:23616-24668)

TCATCTTAAATCATAGGAAAGGAAGATCATCATATTTTTTGAAAAGTAATTTTTTAA CGCATGATCTATGATTTCAGGGTCCG

TGCTTTTAGGCAACGGGATGGTGGTCGGACTATAAATCTTTAGAGATAAAATGTTTT TTATAAGCTCATACCCTTCCCCTAAA

GCTGTAGTACCCTCTTCGAAAACATCAGCCCCCAGATCCATACAAAAGAACATGTTT TCTATATTATAGTACTGTATTGAGGT

AAGCATGGCCTGATTGATGTTGGCGCCCAACATATAGCAGTAGTACATGGTTGAAAG GTTGTGGTCTTTGATGCAGGCAATCC

GCATCATCTCTTCTATGTCCATATGGATCTTGTCCTTTTCATACGCCTCATGGAGGT CAAACACATTATTAAAACAAAGAGCA

CATGTTAACCGCCACGTATTCATGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAGGCTATAGCGTACGGCTAT

AGCGTACCAGTATTTGAGTAGTAATGTACTGAGCAAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTAACTCCCTTAACT

CCCAGAAAATTTCTATCCTCAATTTCAGATTATTTACTTTTTGTAATATCGGATTGT TGGAAAACACCTCATGGCATAAAATA

ATGTTACTACTAGTTTTATGAAACTTTAGATCTATGAAAATTTGTAAAATTTCCTCT TCATTCAAGATCTCCTTGGCACCTAA

CTCTTGACAGAGGTCCCGGGTATGCTCCGTGTTGACAGATACCAGCCCATAGTTGAT GTCCGCCCCCCACTCTGTAAATAGTT

TTATAAGGCTGTAGTTGTTTTCCCTTACAGCCTTTACTAACGCCGTATTTAGGTCTA AGCCCTCTTTAATGCCTGATGATTTT ATGAGCCTTAGGTTATGATCAAACGTGATCGGAGCATTATACCACCATAGATCATAATAT TTTAAAAGATAATGTTGGTTCGT

GGGCACGCATTGTCCAGCCAACACCTTTTTGGTCAAAGATTGCAGGGAAGGCAACAT

SEQ ID No. 97 - Malawi Lil-20/1 (1983) MGF 360 12L (AY261361.1 :27682-28731)

TCATCTTAAATCGTAGGAAAGGAAGGTCATCATATTTTTTGATTTATAATTTTTTAA CACATGATCTATTATTTCAGGGTCCG

TGTTTTTAGGTAACGGGGCAGTGGACGGACTATAGATCTTTAGGGATAAAATGTTCC TTATAAGCTCATACCCTTCCCCTACA

GGCATATTCTCTTCGAAAACATCAGCCCCCAGATCCATACAAAAGAACATGTTTTCT ATATTATAGTACTGTATTGAGGCAAG

CATGGCCTGATTAATGTTGGCGCCCAGGACATAGCAGTAGTATATCGTTGAAAGGTT GTGGTCTTTGATGCAGGCTACTCGCA

TCATCTCTTCTAAGTCCATATAGATCTTGTCCTTTTCATACGCCTCATGAAGGTCAA ACACATTATTAAAACAAAGAGCACAT

GTTAACCGCCACGTATTCAGGTGTGTATATTTTTGATAAAAATACTGTATGGCCTCT TTCAGGTTATAGCGTACGGCTATAGC

GTACCAGTATTTGAGTAATAATGTATTGAGTGAAAACTCATTATTTAACAGATCGGT TTTTTTTATTAACTCCCTTAACTCCC

AGAAAATTTCTATCCTCATTTTCAGATTATTTACTTTTTGTAATAACGGATTGTTGG AAAACACCTCATGGCATAAAATAATG

TTACTACTAGTTTTATAAAACTTTAGATCGATGAAAATTCGTAAAATTTCCTCTTCA TTTAAGGTCTCCTTGGCGCCTAGCTC

TCTGCAGAGGTCCCGGGTGTGCTCCGTGTTGACAGATACCAGCCCGTAGTTGATGTC CGCCCCCCACTCTGTAAAAAGCTTTA

TAAGGTTGTAGTTGTTTTCCCTTACAGCCTTTACTAACGCCGTATTTAGGTCTAAGC CATCTTTAATGCCTGCTGATTTTATC

AGCCCTAGGTTATGATCAAACGTGATCGGAGAATCATACCACCATAGGTCGTAACAC TTTAAAAGATAATATTGGTCCGTCGG

CAGGCATTGTCCAGCCAGTACCTTTTTGGTCAGAGATTGCAGGGAAGGCAACAT

SEQ ID No. 98 - Kenya 1950 MGF 360 12L (AY261360.1 :33549-34604)

TCATCTTAGATCATAAGTAAGGAAGACCATCATATTTTTTGAAAAGTAATTTTTTAA CTCATGATCTATAATTTCAGGGTCCG

TGCTTTTAGGCAACGGGGTGGTGGCCGGGCTATAAATCTTTAGGGATAAAATATTCC TTATAGGCTCATACCCTTCCTGTCCC

ACTAGAGCCATACCCTCTTCAAAAGCATCGGCCCCCAGATCCATACAAAAGAATATG TTTTCTATATTATAGTACTGTATTGA

GGTAAGCATGGCCTGATTGATGTTGGCGCCCAGCATATAGCAGTAGTATATCGTTGA AAGGCTGTGGTCTTTGATGCAGGCTA

TCCGCATCATCTCTTCTAAATCCATACAGATCTTGTCCTTTTCATACGCCTCATGAA GGTCAAACACGTTATTAAAACAAAGA

GCACATGTTAATCGCCACGTATTCAGGTGCGTATATTTTTGGTAAAAATACTGTATG GCCTCTTTCAAGTTGTAGCGTACGGC

TATAGCGTACCAGTATTTGAGTAATAGTGTATTGAGCGAAAACTCATTATTTAGCAG ATCGGTTTTTTCTATTATCTCCCTTA

ACTCCCAAAAAATTTCTATCCTCATTTTCAGGTTATTTACCTTTTTTAAAAGTAGAT TATCGGAAAACACCTCATGACATAAG

ATAATGTTACTACTGGTTTTATGAAACTTTAAATCTATGAAAATTTGTAAAATATCC CCTTCATTTAAGGTCTTCTTGGCGCC

TAGCTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATATCAGCCCGTAGTT GATGTTCGCTCCCCACTCCGTAAATA

GTTTTATAAGACTGTAGTTGTTTTCCTTTACAGCCTTCACTAATGCCATGTTTAGGT CTAAGCCTTCTTGAAGGCCTGCTGAT

TTTATCAGCCTTAGGTTATGATCAAACGTGATCGGAGCATTATTCCACCATAGATCG TAACACTTTAAAAGATAATGTTGGTC

CTCGGGCAGACATTGTCCAGCCAGCACCTTTTTGGTCAGAGATTGCAGGGAAGGCAA CAT

SEQ ID No. 99 - Ken06.Bus MGF 360 12L (NC_044946.1:27428-28483)

T CAT C T T AGAT CATAAG TAAG GAAGAC CAT CAT AT t T T T T G AAAAGT AAT T T T T T AAC G CAT GAT CT AT AAT T T CAG G G T C C G TGCTTTTAGGCAATGGGGTAGTGGCCGGGCTATAAATCTTTAGGGATAAAATATTCCTTA TAGGCTCATACCCTTCCTGTCCC ACTAGAGCCATACCCTCTTCAAAAGCATCGGCCCCCAGATCCATACAAAAGAATATGTTT TCTATATTATAGTACTGTATTGA GGTAAGCATGGCCTGATTGATGTTGGCGCCCAGCATATAGCAGTAGTATATTGTTGAAAG GTTGTGGTCTTTGATGCAGGCTA TCCGCATCATCTCTTCTAAATCCATACAGATCTTGTCCTTTTCATACGCCTCATGAAGGT CAAACACGTTATTAAAACAAAGA GCACATGTTAACCGCCACGTATTCAGGTGTGTATATTTTTGGTAAAAATACTGTATGGCC TCTTTCAAGTTGTAGCGTACGGC TATAGCGTACCAGTATTTGAGTAATAGTGTATTGAGCGAAAACTCATTATTTAGCAGATC GGTTTTTTCTATTATCTCCCTTA ACT CCCAAAAAATTTCTATCCTCATTTTCAGGTTATTTACCCTTTTTAAAAGTAAATTATCGG AAAACACCTCATGA CATAAG ATAATGTTACTACTGGTTTTATAAAATTTTAAATCTATGAAAATTTGTAAAATATCCCCT TCATTTAAGGTCTTCTTGGCGCC TAGCTCTCGACAGAGGTCCCAGGTGTGCTCCGTGTTGACAGATATCAGCCCGTAGTTAAT GTTCGCCCCCCACTCCGTAAATA GTTTTATAAGACTGTAGTTGTTTTCCTTTACAGCCTTCACTAATGCCATGTTTAGGTCTA AGCCTTCTTGAATGCCTGCTAAT TTTATCAGCCTTAGGTTATGATCAAACGTGATCGGAGCATTATTCCACCATAGATCGTAA CACTTTAAAAGATAATGTTGGTC CTCGGGCAGACATTGTCTAGCCAGCACCTTTTTGGTCAAAGATTGCAGGGAAGGCAACAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 12L. Suitably the functional version of MGF 360 12L comprises the sequence of SEQ ID No. 88, 89, 90, 91, 92, 93, 94 95, 96, 97, 98 or 99. Suitably the functional version of MGF 360 12L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 88, 89, 90, 91, 92, 93, 94 95, 96, 97, 98 or 99. Suitably the functional version of MGF 360 12L consists of the sequence of SEQ ID No. 88, 89, 90, 91 , 92, 93, 94 95, 96, 97, 98 or 99. MG F 36013L gene sequences

SEQ ID No. 100 - Georgia 2007/1 MGF 360 13L (LR743116.1:31568-32629)

CTATAGTATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAATC TTTTAATAAACTGCTTATTTCTTCGG

GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGTAAATATCCAAAGCCAAAC TATGGCTCAGAAAATTATAACCTTTT

TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAA AATATATTACCGATGTTATAATATTG

TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGCGCGTAACAGTAATATAT TGTTAATGGATTGTTATCCTTGGTAG

AAGCCAGACATATCATGTCATGGACGTCTATTTGGATGTTTTCCTTGTGGTACATCT CATGAAGCTCATATATTTTGTTATAA

TACAGGAGACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCATCTAGAAAA CAAATGGCGTCCTTACAATCGTATTG

TACTGCTTTGGCGTACCAATACTTCACTAGTAAACCATTTAACTCGTCCGTTTCTTT TATTTCTATGAGCCCCCATAGTCTTT

TATAAATTAAGCCCCTTAATTGTATAACAAATTTGTTTTCTAAAATAGGATTATTCA TAAAAATTTCATGGCACAAAATAATA

CTGCCGCTGGTTTTATTGTGCATTATCCTGGTAAAAATACGGAAAATATCGTTGTCC TCTAGAGTTTCTTTGGCGCCTAGCTG

TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACCATAGTTGATATT TGCGCCCCACTCTGTAAAGAGCTTTA

TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACGAAGGTCTATAT CTTCTCCTAAAAATCCTGATTTTATT

TGTATTCGGCCACGATCCATACAAAGCTTGAGAGGAGCATCATGCCACCATAGGCCA CAATATTTCAAAATGCAGTGTTCATC

TATTGACAAACACTGGCTGGCTATCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGG CAACGACAT

SEQ ID No. 101 - China/2018/AnhuiXCGQ MGF 360 13L (MK128995.1 :30597-31658)

CTATAGTATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAATC TTTTAATAAACTGCTTATTTCTTCGG

GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGTAAATATCCAAAGCCAAAC TATGGCTCAGAAAATTATAACCTTTT

TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAA AATATATTACCGATGTTATAATATTG

TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGCGCGTAACAGTAATATAT TGTTAATGGATTGTTATCCTTGGTAG

AAGCCAGACATATCATGTCATGGACGTCTATTTGGATGTTTTCCTTGTGGTACATCT CATGAAGCTCATATATTTTGTTATAA

TACAGGAGACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCATCTAGAAAA CAAATGGCGTCCTTACAATCGTATTG

TACTGCTTTGGCGTACCAATACTTCACTAGTAAACCATTTAACTCGTCCGTTTCTTT TATTTCTATGAGCCCCCATAGTCTTT

TATAAATTAAGCCCCTTAATTGTATAACAAATTTGTTTTCTAAAATAGGATTATTCA TAAAAATTTCATGGCACAAAATAATA

CTGCCGCTGGTTTTATTGTGCATTATCCTGGTAAAAATACGGAAAATATCGTTGTCC TCTAGAGTTTCTTTGGCGCCTAGCTG

TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACCATAGTTGATATT TGCGCCCCACTCTGTAAAGAGCTTTA

TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACGAAGGTCTATAT CTTCTCCTAAAAATCCTGATTTTATT

TGTATTCGGCCACGATCCATACAAAGCTTGAGAGGAGCATCATGCCACCATAGGCCA CAATATTTCAAAATGCAGTGTTCATC

TATTGACAAACACTGGCTGGCTATCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGG CAACGACAT

SEQ ID No. 102 - Tengani 62 MGF 360 13L (AY261364.1:25753-26814)

CTATAGTATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAATC TTTTAATAAACTGCTTATTTCTTCGG GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGTAAATATCCAAAGCCAAACTAT GGCTCAGAAAATTATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGATGTTATAATATTG TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGCGCGTAACAGTAATATATTGT TAATGGGTTGTTATCCTTGGTAG AAGCCAGACATATCATGTCATGGACGTCTATTTGGATGTTTTCCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTCCTTACACTCGTATTG TACTGCTTTGGCGTACCAATACTTCACTAGTAAACCGTTTAACTCATCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT T AT AAAT TAAG C C C C T T AAT T GT AT AG TAAAT T TAT C T T CT AT AT TAG GAT TAT T CATAAATATAT CAT G G CACAAAAT AATA CTGCCGCTGGTTTTATTGTGCATTATCTTGGTGAAAATACGGAAAATATCGTCGTCTTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACTATAGTTGATATTTGC GCCCCACTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCTATATGGAGGTCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTTGGCCACGATCCATACAAAGCTTGAGGGGGACATCATGCCACCATAGGCCACAG TGTTTCAGAATGCAGTGTTCATC TATCGACAAAGACTGGTTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGGTAA TGACAT

SEQ ID No. 103 - Warthog MGF 360 13L (AY261366.1:27277-28338)

CTATAGTATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAATC CTTTAATAAACTGCTTATTTCTTCGG GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGTAAATATCCAAAGTCAAACTAT GGCTTAGAAAATGATAACCTTTT TGTTCCGCTATAGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGATGTTATAATATTG TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCGTAACAATAATATATTGT TAATGGATTGTTATCCTTGGTAG AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTTTCCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTCCTTACACTCGTATTG TACTGCTTTGGCGTACCAATACTTCACTAGTAAATCGTTTAACTCATCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT T AC AAAT TAAG C C C C T T AAT T GT AT AG TAAAT T T G T T T T CT AT AT TAG GAT TAT T CATAAATAT C T CAT G G CACAAAAT AATA CGGCCGCTGGTTTTATTGTGCATTATCTTGGTGAAAATACGGAAAATATCGTTGTCCTCT AGAGTTTCTTTGGCACCTAGCTG TCTACACAACTCTCGGATGTGCTCCGTATTGATAGAAAGCAAACTATAGTTGATATGTGC GCCCCATTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCTATATGGAGGTCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTTGGCCACGATCCATACAAAGCTTGAGGGGGACATCATGCCACCATAGGCCACAG TGTTTCAGAATGCAGTGTTCATC

TGTCGACAAACACTGGCTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGG CAACGACAT

SEQ ID No. 104 - Warmbaths MGF 360 13L (AY261365.1:30498-31559)

CTATAGTATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAACC CTTTAATAAACTGCTTATTTCTTCGG GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGTAAATATCCAAAGCCAAACTAT GGCTTAGAAAATGATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGATGTTATAATATTG TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCGTAACAGTAATATATTGT TAATGGATTGTTATCCTTGGTAG AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTATCCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCATTCATTAAGACCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTCCTTACACTCGTATTG TACTGCTTTGGCGTACCAATACTTCACTAGTAAATCGTTTAACTCATCCGTTTCTTTTAT TTCTATGAGCCTCCATAGTCTTT T AC AAAT TAAG C C C C T T AAT T GT AT AG TAAAT T T G T T T T CT AT AT TAG GAT TAT T CATAAATAT C T CAT G G CACAAAAT AATA CGGCCGCTGGTTTTATTGTGCATTATCTTGGTGAAAATACGGAAAATATCGTTGTCCTCT AGAGTTTTTTTGGCACCTAGCTG TCTACACAACTCTCGGATGTGTTCCGTATTGATAGAAAGCAAACTATAGTTGATATGTGC GCCCCATTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCTACATGGAGATCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTTGGCCACGATCCATACAAAGCTTGAGGGGGACATCATGCCACCATAGGCCACAG TGTTTCAGAATGCAGTGTTCATC TGTCGACAAACACTGGCTGGCTACCGTTTTTTTGACGAGGGTCTGCAGAGAGAGCGGCAA CGACAT

SEQ ID No. 105 - Pretorisuskop/96/4 MGF 360 13L (AY261363.1 :31307-32368)

CTATAGTATATTATGAGAATATTCCCAAATTATGGATAAGTTTTTTGATTTATAATC CTTTAAAAAACTGCTTATTTCTTCGG GGTCCTTTAAGTTTAGTGGCAAGGAAGCATCTGAGCTGAAAATATCCAAAGCCAAACTAT GGCTTAGAAAATTATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGATGTTATAATATTG TATTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCGTAACAGTAATATATTGT TAATGGATTGTTATCCTTGGTAG AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTTTCCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTAAAA TACAGGAGACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCTTCTAGAAAGCAA ATGGCGTCCTTACACTCGTATTG TACTGCTTTGGCGTACCAATACTTCACTAGTAAATCGTTTAACTCATCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT T AC AAAT TAAG C C C C T T AAT T GT AT AG TAAAT T T G T T T T CT AT AT TAG GAT TAT T CATAAATATAT CAT G G CACAAAAT AATA CGGCCGCTGGTTTTATTGTGCATTATCTTGGTGAAAATACGGAAAACATCGTCGTCTTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAACTCTCGGATGTGCTCCGTATTAATAGAAAGCAAACTATAGTTGATATGTGC GCCCCATTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCTATATGGAGGTCTATATCCT CTCCTAAAAATCCTGATTTTATT TGTATTTGGCCACGATCCATACAAAGCTTGAGGGGGACATCATGCCACCATAGGCCACAG TGTTTCAGAATGCAGTGTTCATC TATCGACAAACACTGGCTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGGCAA CGACAT

SEQ ID No. 106 - Mkuzi 1979 MGF 360 13L (AY261362.1:32286-33347)

CTATAATATATTATGAGAATATTCCCAAATGATGGATAAGTTTTTTGATTTATAATC TTTTAATAAACTGCTTATTTCTTCGG GGTCCTTTAAGTTTAATGGCAAGGAAGCATCTGAACTGTAAATATCCAAAGTCAAGCTAT GGCTTAAAAAATTATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCCCCCAAATCTATACAGAAAAAT ATATTACCGATGTTATAATATTG TACTGAAGTGAGCATAGCTTGGTTGATGTTGCTCCCCAGGGCGTAACAGTAATATATTGT TAATAGATTGTTATCCTTGGCAC AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTTTTCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCATTCGTTAAGATCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTTCTTACAAACGTATTG TACTGCTTTGGCATACCAATACTTCACTAGTAAATCATTTAACTTGTCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT TAT AAATTAAGCCCCTTAATT GTATAACAAATTTGTTTTCTAAAATAGGATTATTCATAAAAATTTCATGGCACAAAAT AATA CTGCCGCTGGTTTTATTGTGCATTATCCTGGTGAAAATACGGAAAATATCGTTGTCCTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACTATAGTTGATATTTGC GCCCCACTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACGAAGGTCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTCGGCCACGATCCATACAAAGCTTGAGAGGAGCATCATGCCACCATAGGCCACAG TATTTCAAAATGCAGTGTTCATC TATTGACAAACACTGGCTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGGCAA CGACAT

SEQ ID No. 107 - L60 MGF 360 13L (NC_044941.1 :25189-26250)

C TATAAT AT AT TAT GAG AATAT T C C CAAAT GAT G GAT AAGT t T T T T GAT T TAT AAT C T T T T AATAAACT G C T TAT TTTTTCGG GGTCCTTTAAGTTTAATGGCAAGGAAGCATCTGAACTGTAAATATCCAAAGTCAAGCTAT GGCTTAAAAAATTATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGACGTTATAATATTG TACTGAAGTGAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCGTAACAGTAATATATTGT TAATAGATTGTTATCCTTGGCAC AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTTTTCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCACTCGTTAAGATCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTTCTTACAAACGTATTG TACTGCTTTGGCATACCAATACTTCACTAGTAAATCATTTAACTCGTCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT TAT AAATTAAGCCCCTTAATT GTATAACAAATTTGTTTTCTAACATAGGATTATTCATAAAAATTTCATGGCACAAAAT AATA CTGCCGCTGGTTTTATTGTGCATTATCCTGGTGAAAATACGGAAAATATCGTTGTCCTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACTATAGTTGATATTTGC GCCCCACTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACGAAGGTCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTCGGCCACGATCCATACAAAGCTTGAGAGGAGCATCATGCCACCATAGGCCACAG TATTTCAAAATGCAGTGTTCATC

TATTGACAAACACTGGCTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGG CAACGACAT

SEQ ID No. 108 - Benin 97/1 MGF 360 13L (NC_044956.1 :24840-25901)

C TATAAT AT AT TAT GAG AATAT T C C CAAAT GAT G GAT AAGT t T T T T GAT T T AT AAT C T T T T AATAAACT G C T TAT TTTTTCGG GGTCCTTTAAGTTTAATGGCAAGGAAGCATCTGAACTGTAAATATCCAAAGTCAAGCTAT GGCTTAAAAAATTATAACCTTTT TGTTCCGCTATGGCACGACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAAAAT ATATTACCGACGTTATAATATTG TACTGAAGTGAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCGTAACAGTAATATATTGT TAATAGATTGTTATCCTTGGCAC AAGCCAGACATATCATGTCATGGACGTCTATTTGGACTTTTTTCTTGTGGTACATCTCAT GAAGCTCATATATTTTGTTATAA TACAGGAGACATTTTAATCGCCACTCGTTAAGATCCGTATATTTCTCATCTAGAAAGCAA ATGGCGTTCTTACAAACGTATTG TACTGCTTTGGCATACCAATACTTCACTAGTAAATCATTTAACTCGTCCGTTTCTTTTAT TTCTATGAGCCCCCATAGTCTTT TATAAATTAAGCCCCTTAATTGTATAACAAATTTGTTTTCTAACATAGGATTATTCATAA AAATTTCATGGCACAAAATAATA CTGCCGCTGGTTTTATTGTGCATTATCCTGGTGAAAATACGGAAAATATCGTTGTCCTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAACTCTCGGATGTGCTTCGTATTGATAGAAAGCAAACTATAGTTGATATTTGC GCCCCACTCTGTAAAGAGCTTTA TCAGACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACGAAGGTCTATATCTT CTCCTAAAAATCCTGATTTTATT TGTATTCGGCCACGATCCATACAAAGCTTGAGAGGAGCATCATGCCACCATAGGCCACAA TATTTCAAAATGCAGTGTTCATC TATTGACAAACACTGGCTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGGCAA CGACAT

SEQ ID No. 109 - Malawi Lil-20/1 (1983) MGF 360 13L (AY261361.1 : 28920-29980)

TTAGAGTATATTATGAGAGTAATCCAAAATGATGGATAAATTTTTTGATTTATAATT TTTTAACAAACTGCTTATTTCTTCGG

GGTCCTTTAAGTTTAATGGCAAGGAAGCATCCGAGCTATAAATATTCAAAACCAAAC TATGGCTTAGAAAATTATAACCTTTT

TGTCTCGCTATGGCACTACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAA TATATATTACCGATGTTATAATATTG

CACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCATAACAGTAATATAT TGTTAATGGATTGTTATCCCTGATAC

AAGCCAGGGATAACATTTCATTAACGTCTATTTGGATTTTTTCCTTGTGGTATATAT CATGAAGCTCATATATTTTGTTATAA

TATAGGTAACATTTTAATCGCCATTCATTAAGATCCGTATATTTCTCATCCAGAAAG CAAATGGCATCTTTATAATCGTATTG

TACTGCTTTGGCGTACCAATATTTCACTAGTAATTCACTTAACTCGTCCGTTTCTTT TATTTCTATAAGCCCGCATAGTCTTT

TATAAATTAAGCGCCTTAATTGTGCAGCAAATTTGTTTTCTAAATTAGGATTATTTA TAAATATCTCATGGCACAAAATAACA

CTGCCGCTGGTTTTATTATGCATTATCTTGTTGAAAATACAGAAAATATCGTAGTCC TCCAGAGTTTCTTTGGCGCCTAGCTG

TCTACACAAATCTCGGACGTGCTTCGTATTGATAGAAAGCAGACTATAGTTGATATT TGCGCCCAACTCTGTAAAAAGCTTTA

TTAGATTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACAAAGGTCTATAT CTTCTCCTAAAAATCCCGATTTTATT

TGTATTCGGCCACGATCCATATAAAGCTTGAGAGGGGCATCATGCCACCATAAGTCA CAGTATTTCAAAATGTGTTCATCTAT

CGACAAACATGATGTACTGGCTACCGTCTTTTTGACGAGGGTTTGTAGAGAGAGCGG CGCCGACAT

SEQ ID No. 110 - Kenya 1950 MGF 360 13L (AY261360.1 :34752-35812)

TTAGAGTATTTTATGAGAGTAATCCAGAATGATGGATAAATTTTTTGATTTATAATT TTTTAATAAACTGCTTATTTCTTCGG G AAC C T T TAAG T T TAAT G G CAAG GAAG CAT C T GAG CT AT AAAT AT T C AAAAT T AAAC TAT G G CAT AAAAAAT T AT AAC CAT T T TGTCTCGCTATGGCACTACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAATAT ATATTACCGATGTTATAATATTG TACTGAAGTAAGCATAGCTTGGTTGATGTTGCCCCCCAGGGCATAACAATAATATATTGT TAATAGATTGTTATCCCTGATAC AAGCCAGAGATAACATTTCATTGACGTCTATTTGGATTTTTTTCTTGTGGTATATATCAT GAAGCTCATATATTTTGTTATAA CATAGGTAACATTTTAATCGCCATTCATCAAGATCCGTATATTTTTCATCCAGAAAGCAA ATGGCATCTTTATGATCGTATTG TACTGCATTGGCGTACCAATATTTCACTAGTAATTCGCTTAACTCGTCCGTTTCTTTTAT TTCTATAAGCCCGCATAATCTTT TATAAATTAAGCGCCTTAATTGTGCAGCAAAGTTGTTTTCTAATTTAGGATTATTTATAA ATATCTCATGGCACAAAATAATA CTGCCGCTAGTTTTATTATGCATTATTTTATTGAAAATACAGAAAATATCGTAGTCGTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAAATCTCGGGCGTGCTTCGTATTGATAGAAAGCAGACTATAGTTGATATATGC GCCCCACTCTGTAAATAACTTTA TCAGACTATAGTTATTTTCCTTAACAGCTATTATTAATGCCACACAAAGGTCTATATCTT CTCCTAGGAATCCCGATTTTATG TATATTCGTCCACGATCTATGTAAAGCTTGAGGGGAGCATCATGCCACCATAAGCCACAG TATTTCAAAATGTGTTCATCTAT CGACAAACATGATGTACTGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGGCGA CGACAT

SEQ ID No. 111 - Ken06.Bus MGF 360 13L (NC_044946.1 :28644-29704)

TTAGAGTATTTTATGAGAGTAATCCAGAATGATGGATAAATTTTTTGATTTATAATT TTTTAATAAACTGCTTATTTCTTCGG GAT C C T T TAAG T T TAAT G G CAAG GAAG CAT C T GAG CT AT AAAT AT T C AAAAC C AAAC TAT G G C T T AAAAAAT TAT AAC CAT T T TGTCTCGCTATGGCACTACCCTCTTCAAAGGCATTACCACCCAAATCTATACAGAAATAT ATATTACCGATGTTATAATATTG TACTGAAGTAAGCATAGCTTGATTGATGTTGCCCCCCAGGGCATAACAATAATATATTGT TAATAGATTGTTATCCCTGATAC AAGCCAGAGATAACATTTCATTGACGTCTATTTGGATTTTTTCCTTGTGGTATATATCAT GAAGCTCATATATTTTGTTATAA CATAGGTAACATTTTAATCACCATTCATCAAGATCCGTATATTTTTCATCCAGAAAGCAA ATGGCATCTTTATGGTCGTATTG TACTGCATTGGCGTACCAATATTTCACTAGCAATTCGCTTAACTCGTCCGTTTCTTTTAT TTCTATAAGCCCGCATAGTCTTT TATAAATTAAGCGCCTTAATTGTGCAGCAAAGTTGTTTTCTAAATTAGGATTATTTATAA ATATCTCATGGCACAAAATAATA CTGCCGCTAGTTTTATTATGCATTATCTTGTTGAAAATACAGAAAATATCGTAGTCGTCT AGAGTTTCTTTGGCGCCTAGCTG TCTACACAAATCTCGGGCGTGCTTCGTATTGATAGAAAGCAGACTATAGTTGATATATGC GCCCCACTCTGTAAATAACTTTA TCATACTATAGTTGTTTTCCTTAACAGCTATTATTAATGCCACACAAAGGTCTATATCTT CTCCTAGGAATCCCGATTTTATG TATATTCGCCCACGATCTATGTAAAGCTTGAGGGGAGCATCATGCCACCATAAGCCACAG TATTTCAAAATGTGTTCATCTAT

CGACAAACATGATGTACCGGCTACCGTCTTTTTGACGAGGGTCTGCAGAGAGAGCGG CGACGACAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 13L. Suitably the functional version of MGF 360 13L comprises the sequence of SEQ ID No. 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110 or 111. Suitably the functional version of MGF 360 13L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110 or 111. Suitably the functional version of MGF 360 13L consists of the sequence of SEQ ID No. 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110 or 111.

MGF 36014L gene sequences

SEQ ID No. 112 - Georgia 2007/1 MGF 360 14L (LR743116.1:32814-33887)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAG ATTTTTTGAAGTATATGTCTTTAGCA TATCATATATTTCCTGGGGTTCGGTTACATCTAATACCAAGGTCACATCACGGCTGAAAA GCTGCTTTACTAAGAAAATGTTG CTCAAGTTATACATATAAGCTTTGTGCGCAATGAGTTGTGCCCTATCAAAATCGGCAGCC CCCAAATCAATACAGAAAAACAT GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCCCAA CCTATGACAGTAATAAATGGCCG CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTGATGCAAATCT CTTTTTCACGACACAACTCGTGT ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTTTTA TGCCTTTTATAGAAATAGTGAAT AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGTGGTG CGCCGTATCCCCCGTCAACGGAA TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGAGTG GATTATGACAAAACATGTCATAA CCCAGCAGAACTCCTGCACAGGATTTTAGCCTGGCCACTTCTTTTAAAATTTCCAGAAGA CGGGGTTCGGATACAGGCGTTAA GCCTCCCAGTTCCGCACACAGCCGCTTTAGATACACGGCAGGAACACGTATAAGCCCATA TTCAGGATTTGCGCCCCAATCCA CAAATAAACGTATAAGTTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTTCGA GACAAAGATCATCCTCAGAAAAA CACTGTAAATGTTTATACGAAAAAACTTGCTTACAATTGTTACATAGGTGAATAGGACCT AAATCCCACCACAAACCAAAACG CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGCCAA CGTTTGTAAAGACAACAT

SEQ ID No. 113 - China/2018/AnhuiXCGQ MGF 360 14L (MK128995.1:31843-32916)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAG ATTTTTTGAAGTATATGTCTTTAGCA TATCATATATTTCCTGGGGTTCGGTTACATCTAATACCAAGGTCACATCACGGCTGAAAA GCTGCTTTACTAAGAAAATGTTG CTCAAGTTATACATATAAGCTTTGTGCGCAATGAGTTGTGCCCTATCAAAATCGGCAGCC CCCAAATCAATACAGAAAAACAT GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCCCAA CCTATGACAGTAATAAATGGCCG CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTGATGCAAATCT CTTTTTCACGACACAACTCGTGT ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTTTTA TGCCTTTTATAGAAATAGTGAAT AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGTGGTG CGCCGTATCCCCCGTCAACGGAA TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGAGTG GATTATGACAAAACATGTCATAA CCCAGCAGAACTCCTGCACAGGATTTTAGCCTGGCCACTTCTTTTAAAATTTCCAGAAGA CGGGGTTCGGATACAGGCGTTAA GCCTCCCAGTTCCGCACACAGCCGCTTTAGATACACGGCAGGAACACGTATAAGCCCATA TTCAGGATTTGCGCCCCAATCCA CAAATAAACGTATAAGTTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTTCGA GACAAAGATCATCCTCAGAAAAA CACTGTAAATGTTTATACGAAAAAACTTGCTTACAATTGTTACATAGGTGAATAGGACCT AAATCCCACCACAAACCAAAACG CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGCCAA CGTTTGTAAAGACAACAT

SEQ ID No. 114 - Tengani 62 MGF 360 14L (AY261364.1:26983-28056)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCAGCTCGTTTCAG ATTTTTTGAAGTATATGACTTTAGCA TATCATATATTTCCTGGGGTTCGGTTACATCTAATGCCAAGGTCACATCACGGCTGAAAA GCTGCTTTACTAAGAAAATGTTG CTCAAGTTATACATATAAGCTTTGTGCGCAATGCGTTGTGCCCTATCAAAATCAGTAGCC CCCAAATCGATGCAGAAAAACAT GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCCTAA CCTATAACAGTAATAAATGGCCG CATAATTTTTTTTCTGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTGATGCAAATCT CTTTTTCACGACACAACTCGTGT ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTTTTA TGCCTTTTATAGAAATAGTGAAT AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGTGGTG CGCCGTATCCCCCGTCAACGGAA TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGAGTG GATTATGACAAAACATGTCATAA CCCAGCAGAACTCCTGCACAGGATTTTAGCCTGGCCACTTCTTTTAAAATTTCCAGAAGA CGGGATTCGGATACAGGCGTTAA GCCACCCAGTTCCGTGCACAGCCGCTTTAGATGCACGGCAGGAACACGTATAAGTCCATA TTCAGGATTTGCGCCCCAATCCA CAAATAAACGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTTCAA GACAAAGATCATCCTCAGAAAAA CACTGTACATGTTTATACGAAAAAATTTGCTTACAATTATTACATAGGTGAATAGGACCT AAATCCCACCACAAACCAAAACG

CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACCTTTTTGGC CAATGTTTGTAAAGACAACAT

SEQ ID No. 115 - Warthog MGF 360 14L (AY261366.1:28495-29568)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAG ATTTTTTGAAGTATATGACTTTAGCA

TATCATATATTTCCTGGGGTTCGGTTACATCTAATGCCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAAAAAATGTTG

CTCAAGTTATACATATAAGTTTTGTGCGCAATGCGTTGTGCCCTATCAAAATCAGTA GCCCCCAAATCGATGCAGAAAAACAT

GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCC TAACCTATAACAGTAATAAATGGCCG

CATAATTTTTTTTCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTGATACAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTT TTATGCCTTTTATAAAAATAGTGAAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGTG GTGCGCCGTATCCCCCGTCAACGGAA

TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGCTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGATACAGGCGTTAA

GCCTCCCAGTTCCGTGCACAGCCGCTTTAGATGCACGGCAGGAACACGTATAAGTCC ATATTCAGGATTTGCGCCCCAATCCA

CAAATAAACGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATACGAAAAAATTTGCTTACAATTGTTACATAGGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAACATATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACCTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 116 - Warmbaths MGF 360 14L (AY261365.1:31747-32820)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAT ATTTTTTGAAGTATATGCCTTTAGCC

TATCATATATTTCCTGGGGTTCGGTTACATCTAATGCCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAAAGAATGTTG

CTCAAGTTATACATATAAGCTTTGTGCGCAATGCGTTGTGCCCTATCAAAATCAGTA GCCCCCAAATCGATGCAGAAAAACAG

GTTTAAAGAATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCTCC CAACCTATAACAGTAATAAATGGCCG

CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTAATGCAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTT TTATGCCTTTTATAGAAATAGTGAAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGCGCAAACCAAAACTTTGTTAATAGGTG GTGCGCCGTATCCCCCATCAACGGAA

TTTTTGAACAGCTGTACATAACTGTGTCTAAAGTGGTTCTAGTTATGGTTTCCAAGA GTGGGTTATAACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGGTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGATACAGGTGTTAA

GCCTCCCAGTTCTGTGCACAGCCGCTTTAGATGCACGGTAGGAACACATATAAGTCC ATATTCAGGATTTGCACCCCAATCCA

CAAATAAACGTATAAGCTCAAGATTATCCCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAGAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATACGAAAAAATTTGCTTACAATTGTTACACAGGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAACATATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACCTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 117 - Pretorisuskop/96/4 MGF 360 14L (AY261363.1 :32525-33598)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAG ATTTTTTGAAGTATATGACTTTAGCC

TATCATATATTTCCTGGGGTTCGGTTACATCTAATGCCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAGAAAATGTTG

CTCAAGTTATACATATAAGCTTTGTGCGCAATGTGTTGTGCCCTATCAAAATCAGTA GCCCCCAAATCGATGCAGAAAAACAT

GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCC TAACCTATAACAGTAATAAATGGCCG

CATAATTTTTTTTCTGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTGATGCAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGGTGATTT TTATGCCTTTTATAGAAATAGTGAAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGCTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGTTACAGGCGTTAA

GCCTCCCAGTTCCGTGCACAGCCGCTTTAGATGCACGGCAGGAACACGTATAAGTCC ATATTCAGGATTTGCGCCCCAATCCA

CAAATAAACGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATACGAAAAAATTTGCTTACAATTGTTACACAGGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACCTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 118 - Mkuzi 1979 MGF 360 14L (AY261362.1:33547-34620)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCCCGTTTCAT ATTTTTTGAAGTATATGTCTTTAGCA

TATCATATATTTCCTGGGGTTCGGTTACATCTAATACCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAGAAAATGTTG

CTCAAGTTATACATATAAGCTTTGTGCGCAATGAGTTGTGCCCTATCAAAATCGGCA GCCCCCAAATCAATACAGAAAAACAT

GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCC CAACCTATGACAGTAATAAATGGCCG

CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTAATGCAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGCTGATTT TTATGCCTTTTATAAAAATAGTGGAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGGTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGATACAGGCGTTAA

GCCTCCCAGTTCCGCACACAGCCGCTTTAGATACACGGCAGGAACACGTATAAGCCC ATATTCAGGATTTGCGCCCCAATCCA

CAAATAAACGTATAAGTTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA CACTGTAAATGTTTATACGAAAAAACTTGCTTACAATTGTTACATAGGTGAATAGGACCT AAATCCCACCACAAACCAAAACG

CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 119 - L60 MGF 360 14L (NC_044941.1 :26422-27495)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAT ATTTTTTGAAGTATATGTCTTTAGCA

TATCATATATTTCCTGGGGTTCGGTTACATCTAATACCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAGAAAATGTTG

CTCAAGTTATACATATAAGCTTTGTGCGCAATGAGTTGTGCCCTATCAAAATCGGCG GCCCCCAAATCAATACAGAAAAACAT

GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCC CAACCTATGACAGTAATAAATGGCCG

CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTAATGCAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGCTGATTT TTATGCCTTTTATAAAAATAGTGGAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGGTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGATACAGGCGTCAA

GCCTCCCAGTTCCGCACACAGCCGCTTTAGATACACGGCAGGAACACGTATAAGCCC ATATTCAGGATTTGCGCCCCAATCCA

CAAATAAACGTATAAGTTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATACGAAAAAACTTGCTTACAATTGTTACATAGGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 120 - Benin 97/1 MGF 360 14L (NC_044956.1 :26073-27146)

TTAGTCTATAACTATAATTTCTGGATGGGCTGTAAGATACTCTTCGGCTCGTTTCAT ATTTTTTGAAGTATATGTCTTTAGCA

TATCATATATTTCCTGGGGTTCGGTTACATCTAATACCAAGGTCACATCACGGCTGA AAAGCTGCTTTACTAAGAAAATGTTG

CTCAAGTTATACATATAAGCTTTGTGCGCAATGAGTTGTGCCCTATCAAAATCGGCG GCCCCCAAATCAATACAGAAAAACAT

GTTTAAAGTATTATTGTTATAGATAGAAAGATTCATGCCATAATCGAGACTAGCCCC CAACCTATGACAGTAATAAATGGCCG

CGTAATTTTTTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTAATGCAAA TCTCTTTTTCACGACACAGCTCGTGT

ATGTCAAAAATGTTATTAAAATAAAGGCTACAAGCTACCCGCCAATAGAGCTGATTT TTATGCCTTTTATAAAAATAGTGGAT

AGCCTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGTTAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TGTTTGAACAGGTGTACGTAACTGTGTCTAAAGTGGTTCTAGTTACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTTAGGTTGGCCACTTCTTTTAAAATTTCCAGA AGACGGGGTTCGGATACAGGCGTCAA

GCCTCCCAGTTCCGCACACAGCCGCTTTAGATACACGGCAGGAACACGTATAAGCCC ATATTCAGGATTTGCGCCCCAATCCA

CAAATAAACGTATAAGTTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATACGAAAAAACTTGCTTACAATTGTTACATAGGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAACGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 121 - Malawi Lil-20/1 (1983) MGF 360 14L (AY261361.1 :30193-31266)

TTAGTCTATAACTACAATTTCTGGATGGGCTGTAAAATACTCTTCGGCTTGTTTTAG ATTTTTTGACGTATATGTATTTAGCA

TATCATATATTGCCTGGGGCTCAGTTAGATCTAATATCAAGCTCACATCACGGCTGA AAAGCTGTTTTACTAAAAAAAGGTTG

CTCAAGTTATACATATAAACTCTACGCGCAATGAGTCGCGCCCTATCAAAGTTAGTA GCCCCCAAATCAATGCAGAAAAATAG

GTTTAAAGTATTATTGTTATAGATAGATAGATTCATGCCATAATCAAGACTAGCCCC TAACATATGACAGTAATAAATGGCTG

CATAATTTTCTTCCCGCAAGCAAGCAAATTTCATCATCAGATTAGGGCTAATGCAAA TCTCTTTTTTACAACACAGCTCATGC

AAGTCAAAAATGTTGTTAAAATAAAGGCTACAAGTCAGTCGCCAATAGAGCTGATTT TTATGCTTTTCGTAGAAATAGTGAAT

AGCTTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTTGATAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TTTTTGAACGGGTGTACATAACTGTGTCTAAGGTGGTTCTAGTCACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTCAGATCGGCCACTTCTTTTAAAATCTCCAGA AGACGAGATTCGGATACAGGTGTTAA

GCCTCCTAGTTCCATGCACAGCCGCTTTAGATGCACGGCAGGAACACGTATAAGTCC ATATTCAGGATTTGCGCCCCAATCCA

CAAACAATCGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCTT CAAGACAAAGATCATCCTCAGAAAAA

CACTGTAAATGTTTATATGAAAAAATTTGCTTACATGTATTACATAAGTGAATAGGA CCTAAATCCCACCACAAACCAAAACG

CTGCAGCGTATAATCATAATCACTTGAAAGATAATTGCATGCCACAACTTTTTTGGC CAGCGTTTGTAAAGACAACAT

SEQ ID No. 122 - Kenya 1950 MGF 360 14L (AY261360.1 :36121 -37194)

TTAGTCTATAACTACAATTTCTGGATGGGCTGTAAAATACTCTTCGGCTCGTTTTAG ATTTTTTGACGTATATGTCTTTAGCA

TATCATATATTGCCTGGGGTTCGGTTATATTTAATACCAAGCTCGCATCACGGCTGA AAAGCTGTTTTACTAAAAAAATGTTG

CTCAAGTTATACATATAAACTCTATGCGCAATGAGTCGCGCTCTATCAAAGTTAGTA GCCCCCAAATCAATACAGAAAAATAG

GTTTAAAGTATTATTGTTATAGATAGATAGATTCATGCCATAATCGGGACTAGCCCC CAACATATGACAGTAATAAATGGCTG

CATAATTTTCTTCCCGCAGGCAAGCAAATTTCATCATTAGATTAGGGCTAATGCAAA TCTCTTTTTTAGAACACAGCTCATGC

AAGTCAAAAATATTATTAAAATAAAGGCTACAAGCCAGCCGCCAATAGAGCTGATTT TTATGCTTTTCATAGAAATAGTGAAT

CGCTTTTGTAAAATTATGTCGTAATGCCAGGGCAAACCAAAACTTCGTTAATAGGAG GTGCGCCGTATCCCCCGTCAACGGAA

TTTTTGAACGGGTGTACATAACGGTGTTTAAAGTGGTTCTAGTCACGGTTTCCAAGA GTGGATTATGACAAAACATGTCATAA

CCCAGTAGAACTCCTGCACAGGATTTCAGATCGGCCACTTCTTTTAAAATTTCCAGA AGACGAGATTCGGAGACAGGTGTTAA

GCCTCCTAGTTCTGTGCACAGCCGTTTTAGATCCACGGCAGGAACACGTATAAGTCC ATATTCAGGATTTGCGCCCCAATCCA

CAAACAATCGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCGT CAAGACAAAGATCATCCTCAGAAAAA CACTGCAAATGTTGATACGAAAAAATTTGCTTACATGTATTACATAAGTGAATAGGACCT AAATCCCACCACAAACCAAAACG

CTGCAGCGTATAATCATAGTCACTTGAAAGATAATTGCATGCCACAACCTTTTTGGC CAACGTTTGTAAAGACAACAT

SEQ ID No. 123 - Ken06.Bus MGF 360 14L (NC_044946.1:29917-30990) TTAGTCTATAACGACAATTTCTGGATGGGCTGTAAAATACTCTTCGGCTCGTTTTAGATT TTTTGACGTATATGTCTTTAGCA TATCATATATTGCCTGGGGTTCGGTTATATTTAATACCAAGCTCGCATCACGGCTGAAAA GCTGTTTTACTAAAAAAATGTTG CTCAAGTTATACATATAAACTCTATGCGCAATGAGTCGCGCTCTATCAAAGTTAGTAGCC CCCAAATCAATGCAGAAAAATAG GTTTAAAGTATTATTGTTATAGATAGATAGATTCATGCCATAATCGGGACTAGCCCCCAA CATATGACAGTAATAAATGGCTG CATAATTTTCTTCCCGCAGGCAAGCAAATTTCATCATTAGATTAGGGCTAATGCAAATCT CTTTTTTAGAACACAGCTCATGC AAGTCAAAAATGTTATTAAAATAAAGGCTACAAGTTAGCCGCCAATACAGCTGATTTTTA TGTTTTTCGTAGAAATAGTGAAT CGCTTTTGTAAAATTATGCCGTAATGCCAGGGCAAACCAAAACTTCGTTAATAGGAGGTG CGCCGTATCCCCCGTCAACGGAA TTTTTGAACGGGTATACATAACTGTGTTTAAAGTGGTTCTAGTCACGGTTTCCATAAGTG GATTATGACAAAACATGTCATAA CCCAGTAGAACTCCTGCACAGGATTTCAGATCGGCCACTTCTTTTAAAATTTCCAGAAGA CGAGATTCGGAGACAGGTGTTAA GCCTCCTAGTTCCGTGCACAGCCGTTTTAGATGCACGGCAGGAACACGTATAAGTCCATA TTCAGGATTTGCGCCCCAATCCA CAAACAATCGTATAAGCTCAAGATTATCGCTCTTCACGGCCTTTACTAGCGCCGCGTCAA GACAAAGATCATCCACAGAAAAA CACTGCAAATGTTGATACGAAAAAATTTGCTTACATGTATTACATAAGTGAATAGGACCT AAATCCCACCACAAACCAAAACG CTGCAGCGTATAATCATAGTCACTTGAAAGATAATTGCACGCCACAACTTTTTTGGCCAA CGTTTGTAAAGACAACAT

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 14L. Suitably the functional version of MGF 360 14L comprises the sequence of SEQ ID No. 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, or 123. Suitably the functional version of MGF 360 14L comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, or 123. Suitably the functional version of MGF 360 14L consists of the sequence of SEQ ID No. 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, or 123.

MGF 36021 R gene sequences

SEQ ID No. 124 - Georgia 2007/1 MGF 360 14L

ATGTCTACTCCACTTTCTCTACAGACTCTTGTTAAAAAAGTGCTGGCCACACAGCAC ATATCTAAAGAACACTACTTTATTTT GAAATATTGTGGTTTATGGTGGCATGAAGCGCCGATTACGATTTGCATTGATGAGGATAG CCAAATATTGATAAAATCGGCAA GCTTCAAAGAAGGCTTATCTTTAGATATCGCATTAATGAAAGTCGTGCAAGAAAATAACC ATGATTTAATAGAGTTGTTTACC AAGTGGGGTGCAGATATCAACTCTAGCTTAGTTACTGTTAATACGGAGTATACCCGGAAC CTTTGTCAGAAATTAGGCGCAAA GGAAGCTTTGAATGAAAGGGATATTTTACAAATATTTTATAAAACACGTCATCTTAAAAC TAGCAGTAATATTATTTTATATA ATGAATTGTTTTCTAATAATCTCCTTTTCCAAAATATAGAGAGATTGAGTTTAATAGTTT ATAGGGGCTTGAAAAACTTATCA ATCAACTTTATATTGGATGATATTTCATTTAGCGAAATGTTAACTAGATACTGGTATAGT ATGGCGATATTATATAACCTTAC TGAAGCCATCCAATATTTTTATCAACGATATAGGCATTTTAAAGATTGGCGGCTTATATG TGGGCTTTCTTTTAACAATTTGT CTGACCTTCATGAAGTATATAACTTAGAGAAGACGGATATAGACATTGATGAAATGATGA AGTTGACCTGTAGTACGTATGAT GGTAATTATTCGACTATTTATTATTGTTTTATGTTGGGGGCTGACATCAATCGGGCAATG TTAACCTCGGTAATAAACTTTCA TATTGGTAACTTGTTCCTTTGTATAGATTTAGGAGCTGATGCTTTCGAAGACAGCATGGA ACTAGCAAAACAAAAGAATAATA ATATATTAGTAGAAATATTATCATTTAAAAATTATTATAGTTCAAATACCTCTCTTTTAT CAATAAAAACGACAGATCCGGAA AAAAT TAAT GC CT TATTAGAT GAAGAAAAGTAT GAGT CAAAAAATAT GT TAAT GTAT GAAGAATTAT CT CATT GA

SEQ ID No. 125 - China/2018/AnhuiXCGQ MGF 360 14L

ATGTCTACTCCACTTTCTCTACAGACTCTTGTTAAAAAAGTGCTGGCCACACAGCAC ATATCTAAAGAACACTACTTTATTTT GAAATATTGTGGTTTATGGTGGCATGAAGCGCCGATTACGATTTGCATTGATGAGGATAG CCAAATATTGATAAAATCGGCAA GCTTCAAAGAAGGCTTATCTTTAGATATCGCATTAATGAAAGTCGTGCAAGAAAATAACC ATGATTTAATAGAGTTGTTTACC AAGTGGGGTGCAGATATCAACTCTAGCTTAGTTACTGTTAATACGGAGTATACCCGGAAC CTTTGTCAGAAATTAGGCGCAAA GGAAGCTTTGAATGAAAGGGATATTTTACAAATATTTTATAAAACACGTCATCTTAAAAC TAGCAGTAATATTATTTTATATA ATGAATTGTTTTCTAATAATCTCCTTTTCCAAAATATAGAGAGATTGAGTTTAATAGTTT ATAGGGGCTTGAAAAACTTATCA ATCAACTTTATATTGGATGATATTTCATTTAGCGAAATGTTAACTAGATACTGGTATAGT ATGGCGATATTATATAACCTTAC TGAAGCCATCCAATATTTTTATCAACGATATAGGCATTTTAAAGATTGGCGGCTTATATG TGGGCTTTCTTTTAACAATTTGT CTGACCTTCATGAAGTATATAACTTAGAGAAGACGGATATAGACATTGATGAAATGATGA AGTTGACCTGTAGTACGTATGAT GGTAATTATTCGACTATTTATTATTGTTTTATGTTGGGGGCTGACATCAATCGGGCAATG TTAACCTCGGTAATAAACTTTCA TATTGGTAACTTGTTCCTTTGTATAGATTTAGGAGCTGATGCTTTCGAAGACAGCATGGA ACTAGCAAAACAAAAGAATAATA ATATATTAGTAGAAATATTATCATTTAAAAATTATTATAGTTCAAATACCTCTCTTTTAT CAATAAAAACGACAGATCCGGAA AAAAT TAAT GC CT TATTAGAT GAAGAAAAGTAT GAGT CAAAAAATAT GT TAAT GTAT GAAGAATTAT CT CATT GA

SEQ ID No. 126 - Tengani 62 MGF 360 14L

ATGTCTACTCCACTTTCTCTACAGACTCTTGTTAAAAAAGTGCTGGCCACACAGCAC GTATCTAAAGATCACTACTTTATTTT AAAATATTGTGGTTTGTGGTGGCATAAGGCCCCGATTACGATTTGCATTGATGAGGATAG CCAAATATTGATAAAATCGGCAA GCTTTAAAGAAGGCTTACCTTTAGATATGGCATTAATGAAAGCTGTACAGGAAAACAACT ATGAAATGATCATGTTGTTTACT GAGTGGGGTGCAGACATTAACTCTAGCTTAGTCACTGTTAATACGGAGCGTACCCGGAAC CTTTGTCGAGAATTAGGCGCGAA GGAAATTTTGAATGAAAAAGAAATTTTAGAAATATTTTATAAAACATGTCATATTAAAAC TAGCAGTAATTTTATTCTATGCC ATGAATTGTTATCCAATAATCCCCTTTTCCAAAATATAGAGAGTTTGAGTTTAATAGTTT ATAGGGGCTTGAAAAACTTATCA ATTAACTTTATATTGGATGATATTTCATTTAGCGAAATGTTAACTAGATACTGGTATAGC TGGGCGATATTATATAACCTTAC TGAAGCTATTCAATATTTCTATCAAACATACAAGCATTTTAAAGATTGGCGGCTAATATG TGGACTTGCTTATAATAACGTGT TTGATCTTCATGAAATTTATAACATAGAAAAGACTGATTTAGATATTGATGAAATGATGC AGTTGGCTTGTATGTATGATAGT AATTATTCAACTATTTATTATTGTTTTATGTTGGGGGCTAATATCAATCAGGCAATGTTA ACCTCGGTAATAAACCATTATAT TAATAACTTGTTCCTTTGTATAGATTTGGGAGCTGACGCCGTCAAAGACAGCATGGAACT AGCAAAACAAAAGAATTATAATA TATTAGTAAAAATATTAACATTTAAAAATTATAGTCCAGACTCTTCTCTTTTATCATTAA AAACGACAGATCCGGAAAAAATT AAT GCCTTACTAGAT GAAGAAAAGTAT GAGT CGAAAAATAT GTTAAT GTAT GAAGAATCAT CT CGTT GA

SEQ ID No. 127 - Pretorisuskop/96/4 MGF 360 14L

ATGCCTTCTCCACATTCTCTACAGACTCTTGCTAAAAAAATACTGGCTACACAGCAG ATATCTACAGATCACTACTTTATTTT AAAATATTGTGGTTTATGGTGGCATGGAGCTCCAATTATGCTTTCTACTAATGAGGATAA TCAATTGATGATAAAATCGGCAA GCTTCAAAGAAGGCTTATCTTTAGATTTGGCATTAATGAAAGTCGTGCAAGAAAACAATC ATGATTTAATAAAGCTGTTTACC GAGTGGGGTGCAGACATCAACTCTAGCTTCGTCACTGTTAATATGGAGTGTACCCGAAAC CTCTGTCGGGAATTAGGCGCGAA GGAAGCTTTGAATGAAAGGGATATTTTACAAATATTTTATAAAACACGTGATATTAAAAC TAGCAGTCATGTTATTTTATGCC ATGAATTATTATCCAATAACCCCCTTTTCCAGAATATAGAGAGAATGCGTTCAATAATTT ATAGGAGTCTGGAAAAACTATCA ATCAACTTTATATTGGATGATATTTCATTTAGTGAAATGTTAACTAGACACTGGTATGGG TTGGCGATATTATATAACCTAAC TGAAGCTATCCAATATTTCTATGAAAAATACAAGCATTTTAAAAATTGGCGGCTAATATG TGGACTTTCTTTTAACAATCTGT CTGATCTTTATGAAATATATAATTTAGAGAAGGTTGATATGAACATTGATGAAATGATGT ATCTGGCCTGTAGTATATATGAT GGTAATTATTCAACTATTTATTATTGTTTTGTATTGGGAGCTGATATCAATCAGGCAATG TTAACCTCAGTAATAAACCATTG TATTGGTAACTTGTTCCTTTGTATAGATTTAGGGGCTGATGCTTTCGAAGACAGCATGGA ACTAGCAAAGCAAAAAAATGATA ATATTTTTATTAGTATATTATCATTTAAAAATTACAGTCCAGACTCTTCTCTTTTGTCAT TAAAAATGACAGATCCGGAAAAA AT TAAT G C C T T AC TAGAT G AAGAAAAG TAT GAG T CAAAAAATAT G T TAAT G T T T GA

SEQ ID No. 128 - Mkuzi 1979 MGF 360 14L

ATGCCTACTCCACTTTCTCTACAGACTCTTGCTAAAAAAGTACTGGCCACACAGTAT ATATCCATAGATTATTACTTTATTTT G AAAT AT T G T G GT T TAT G G T G G CAT G GAG C C C C TAT TAT GAT T T C CAT T GATAAG AATAAAAG C C AAAT AC T GAT AAAAT C G G CAAGCTTCAAAGAAGGCTTATCTTTAGATATCGCATTAATGAAAGCTGTGCAGGAAAACA ATCATGATTTAATAAAGCTGTTT ACCGAGTGGGGTGCAGACATCAACTCTAGCTTAGTCACTGTTAATACGGAGTGTACCCGA AACCTCTGTCGGGAATTAGGCGC GAAGGAAGCTTTGAATGAAAGGGATATTTTACAAATATTTTATAAAACACGTGATATTAA AACTAGCAGTAATGTTATACTAT GCCATGAATTGTTATCCAATAACCCCCTTTTCCAAAATATAGAGAGAATGCGTTCAATAA TTTATAGGAGTCTGGAAAAACTA TCAATTAACTTTATATTGGATGATATTTCATTTAGCGAAATGTTAACTAGACACTGGTAT AGGTTGGCGATATTATATAACCT AACTGAAGCTATCCAATATTTCTATGAAAAATACAAGCATTTTAAAGATTGGCGGCTAAT ATGTGGGCTTTCTTTTAACAATC TGTCTGACCTTCATGAAATATATAACTTAGAGAAGGTTGATATGGACATTGATATAATGA TGTATCTGGCCTGTAGTACGTAT GATGGTAATTATTCAACTATTTATTATTGTTTTGTATTGGGGGCTGATATCAATCAGGCA ATGTTAACCTCGGTAATAAACCA TCGTATTGGTAACTTGTTCCTTTGTATAGATTTAGGGGCTGATGCCTTCGAAGACAGTAT GAAACTAGCAAAACAAAAGAATC ATAATATATTAGTACATATATTATCGTTTAAAAACTATAGTCCAGACTTCTCCCTTTTAT CAATAAAAACGACAGATCCAGAG AAAAT TAAT GC CT TACTAGAT GAAGAAAAGTAT GAGT CAAAAAATAT GT TAAT GTAT GAT GAATTT GAC GC CT GTACTAATAA TGTTTAA

SEQ ID No. 129 - Warmbaths MGF 360 14L

ATGCCTACTCCACTTTCTCTACAGACTCTTGCTAAAAAAGTACTGGCCACACAGTAT ATATCCATAGATTATTACTTTATTTT G AAAT AT T G T G GT T TAT G G T G G CAT G GAG C C C C TAT TAT GAT T T C CAT T GATAAG AATAAAAG C C AAAT AC T GAT AAAAT C G G CAAGCTTCAAAGAAGGCTTATCTTTAGATATCGCATTAATGAAAGCTGTGCAGGAAAACA ATCATGATTTAATAAAGCTGTTT ACCGAGTGGGGTGCAGACATCAACTCTAGCTTAGTCACTGTTAATACGGAGTGTACCCGA AACCTCTGTCGGGAATTAGGCGC GAAGGAAGCTTTGAATGAAAGGGATATTTTACAAATATTTTATAAAACACGTGATATTAA AACTAGCAGTAATGTTATACTAT GCCATGAATTGTTATCCAATAACCCCCTTTTCCAAAATATAGAGAGAATGCGTTCAATAA TTTATAGGAGTCTGGAAAAACTA TCAATTAACTTTATATTGGATGATATTTCATTTAGCGAAATGTTAACTAGACACTGGTAT AGGTTGGCGATATTATATAACCT AACTGAAGCTATCCAATATTTCTATGAAAAATACAAGCATTTTAAAGATTGGCGGCTAAT ATGTGGGCTTTCTTTTAACAATC TGTCTGACCTTCATGAAATATATAACTTAGAGAAGGTTGATATGGACATTGATATAATGA TGTATCTGGCCTGTAGTACGTAT GATGGTAATTATTCAACTATTTATTATTGTTTTGTATTGGGGGCTGATATCAATCAGGCA ATGTTAACCTCGGTAATAAACCA TCGTATTGGTAACTTGTTCCTTTGTATAGATTTAGGGGCTGATGCCTTCGAAGACAGTAT GAAACTAGCAAAACAAAAGAATC ATAATATATTAGTACATATATTATCGTTTAAAAACTATAGTCCAGACTTCTCCCTTTTAT CAATAAAAACGACAGATCCAGAG AAAAT TAAT GC CT TACTAGAT GAAGAAAAGTAT GAGT CAAAAAATAT GT TAAT GTAT GAT GAATTT GAC GC CT GTACTAATAA TGTTTAA

SEQ ID No. 130 - Ken05/Tk1 MGF 360 14L ATGCCTACACCGTCTTCTTTACAAGTCCTTGTCAAAAGGGTGTTGGACTACCAGCATCAT GTATCTGAAGATGATTATTGTAT TTTACAGCATTGTGGGTTGTGGTGGCATGGAAGTCCAATTATGTTTTTTACTAATGAAAA TCATCAAAGGATGATAAAATCAG CAAGCTTTAAAGATGGTTTAGAAATAAATCTCGCATTAATGAAGGCCGTACAGGAAAACA ATTGTATCTTAATAGAGCTGTTT ACCGAATGGGGTGCAGACATTAATTATGGATTGATTACTGTCAATACGGAATATACCCGA AACCTATGTCGAAATCTAGGTGC CAAGGAGGCGTTAAATACACGGAAAATTTTAGATGTATTTTTAAATTTAAAGGATTTTAA AAGTAGTAATCATATTATTCTAT G C CAT GAAT T G T TAT CT AATAAT CCTCTTTTAC TAAG T G AAAATAAT GAT TAT T T GAGAAAAATAAT TAAT T G TAAT CT AAG G

AGAATATCAATTAACTTTATATTGGATGAAATTTCATTTAATGAAAAGCTAACCAGA TTCTGGTATAAACAGGCAGTACTAAA TAATCTTACTGAAGCTATCCAATATTTCTATCAAAAATATAAGCAGTTTAAAGACTGGCG ACTAATATGTGGACTTGCTTTAA ATAATGTATTTGATCTTCATGAAATATATAACAAAGAGAAGGCTGATATAGATATTAATC AAATGATAGAGATAACCTGTACA TATATGTGTAGTTATCCCACCATTTACTATTGTTTTGTAATGGGGGCTGACATTAATCGG GCAATGATTACCTCAGTTACAAA ATCTTATACTGGTAACTTGTTCTTTTGTATAGATTTAGGAGCTACTGCCTTCGAAGAGTG CTTAGAAATAGCAAAACAACAGA ATGATAATGAATTGGTAAAGGTATTATCATTGAAAAACTATTACAGTCCAGATAGCTCCC TTTTATCATTAAAAATAACAGAT CCGGAAAAAAT TAAT AT CT TATTAGAT GAT GAAACATAT GAGT CAAAAAAT GAGT TAAT AT AT GAAG AAT T AATATAA

SEQ ID No. 131 - Kenya 1950 MGF 360 14L

ATGTCCACACCATCTTCTTTACAAGTCCTTGTCAAAAGGGTGCTGGACTTCCAGCAT GTGTCTGAAGATGATTACTGTATTTT AAAAT GTTGTGGGTTATGGTGGCATGGAGGCCCAATCATGCTTTCTACGAATGAGGATAATCAAA TGATGATAAAATCAGCAA

GTTTTAAAGATGGTTTAGAGATAAATCTCGCATTAATGATGGCCGTACAAGAAAACA ATTGTAGCCTAATAGAGCTGTTTACC GAATGGGGTGCAGACATTAATTCTGGATTGGTTACTGTCAACACGGAATACACCCGAAAC CTATGTCGAAATCTAGGTGCTAA GGAGACATTAAATAAACGGGAAATTTTAGATGTATTTTTAAAATTAAAGAATTTTAAAAG TAGTAATAATATTATTCTATCCC ATGAATTATTATCTAATAATCCTCTTTTCCTAAGTGAAGATAATGATTATTTTAGAAGAA TAATTAATTGTAATCTAAGGAGA ATATCAATTAACTTTATATTGGATGAAATTTCATTTAATGAAAAGCTAACCAGATTCTGG TATAGTCAGGCGGTACTATATAA TCTTACTGAAGCTATCCAATATTTCTATCAAAAATATGAACATCTCAATGAGTGGCGTTT AATTTGTGCGCTTTCTTTTAATA ATGTGTTTGATCTTCATGAAATATATAACAAAGAAAAGGTTGGTATGGATATTAATCAAA TGATAGAGATAACCTGTGCATAT ATGTGTAGTTATTCCACCATTTACTATTGTTTTGTAATGGGAGCTGACATTAATCGGGCA ATGATTATCTCGGTTACAAAATC TTATACTTATAACTTGTTCTTTTGTATAGATTTAGGAGCTACTGCCTTCGAAGAGTGTTT AGAAATAGCAAAACAACAGAATA ATAATGAATTAGTAAAAATATTATCATTGAAAAACTATTATAGTCCAGATAGCTCCCTTA TATCATTAAAAATAACAGATCCG GAG AAAAT T AATAT C T TAT TAGAT GAG GAAAAT TAT GAG T CAAAAAAT GAG T T AATATAT G AAGAAT CT AATATAAATAAT AG C GAT GAT AT AT T T T GA

SEQ ID No. 132 - Ken06.Bus MGF 360 14L

ATGCCATCTACTTTACAAGCACTTGCTAAAAAAGTACTGGCCACACCGCATATATCT AAAAAATATTGTCAGTCTAAGGAGTA C T G T CAT AT AT TAAAAT GTTGTGGTT TAT G G T G G CAT GAT G CT C C AAT T AC AAT T TAT C CT T G TATAAATAAAAT AT T GAT AA

AAACAGCAAGCTTTAAACATGGTTTAGAGTTGAATGTCGCATTCATGAAAGCTGTAC AGGAAAATAATTATGATCTAATAAGG CTGTTTATTGAGTGGGGTGCGGATATCAACTTTGGGTTGGCCACTGTTAATACAGACCGT ACCCGGGACCTGTGCCGGGAACT AGGTGCGAAGGAAGCTTTAAGTGAAAAAGAAATTTTAGAAATATTTGATAAAATACAGTA TATTAAAAGTAGCAGTAATATTA TTGTATCCCATGAATTATTATCTAATAACCCCCTTTTCTTAAATAATGATCAATTGAAAT TAAGAATGTTCGATGAACTACAT AAACTATCAATCAACTTTGTATTACATGAGATATCATTTAATGAAATGCTAACTAGGTAT TGGTATAGTATGGCGATACTATA TAACCTCACCGCAGCCATTCAATATTTTTATCAATCCTACAAGCATTTTAAAGATTGGCG GTTAATATGTGGGCTTGCTTATA ACAACGTGTTTGATCTTCATGAAATATATAACAAAGAGAAGGTTGATATGGATATTAATC AAATGATGCAGTTGGCCTGTATG TATGATGGTAATTATACAACCATTTATTATTGTTTTATGTTGGGAGCTGACATTAATCGG GCAATGATTACCTCGGTTACAAA ATCTTGTGATGGTAACTTATTCCTTTGTATAGATTTGGGGGCTGATGTATTTGAAGAGAG TATGAAAATGGCAATAGAAGATC ATAATGACGTATTAGAAAGTATCTTATCATTTAAAAATTATTATAGTTCAGACGTTTCTC TGTTATCATTAAAGACGACAGAT CCA GAAAAAATTAATGCCTTGTTAGAAGAAGAAAGTTACAAAT CAAAAAAT AGGTTGAT AT AT AAAAGTTATTGA

SEQ ID No. 133 - Malawi Lil-20/1 (1983) MGF 360 14L

AT GAT G C CAT C TACT T T AC AAACAC T T G C TAAAAAAACAT T G G C CACACAG CATATAT C TAAAAAAT AT TGGCCGTCT GAG GA GTATTGTTATATATTAAAATGTTGTGGTTTATGGTGGCATGATTCTCCAATCACGATTTT TACTTGTATAAAACAAATATTAA TAAAAACAGCAAACTTTAAACATGGTTTAGACTTAAACCTTGCATTAATGAAAGCTGTAC AAGAAAATAACTATGAATTGATC ATGTTGTTTACTGAGTGGGGTGCGGATATCAACTTGGGGTTGATCACTGTTAATACAGAG TGCACCCGAGATTTGTGCCAAAA GTTAGGTGCAAAGGAAGCTTTGAGTGCAAAGGAAATTTTAGAAATATTTTATAAAATACA GTATATTAAGAGTAGCAATAATA TTATTATATCTCATGAATTAATATCTAATCATCCCCTTTTCCTAAATAATGATCAATTGA AATTAAGAATTGTTGGTGAACTA AATGCTATATCAATCAACTTTATATTGGATGAGATATCATTCAATGAAATGCTAACTAGG TATTGGTATAGTATGGCGATACT ATATAAACTCCCTGCAGCCATTCAATATTTTTATCAATCATACAAGTATTTTAAAGATTG GCGGTTAATATGTAGCCTTGCTT ATAACAACGTGTTTGACCTTCATGAAATTTATAATAAAGAGAAGACTGATATAAACATTG ATGAAATGATGCGGTTGGCCTGT AGGTATGATGGTAATTATACAACTATTTATTATTGTTTTATGTTGGGGGCTGACATTAAT CAGGCAATGATTACCTCGGTCAT GAATCTTTGGGACGGTAACTTATTTCTTTGTATAGATTTGGGGGCTGATGTATTTGAAGA GTGTATGAAAATAGCAATAGAAG ATCATAATGGTGTATTAGAAAGTATCTTATCATTTAAAAATTATTATAGTCCAGACGTTT CTCTGTTATCATTAAAAACGACA GAT C C G G AAAAAAT T AAT GAC T T G C TAGAT G AAGATAT C TATAAAT C AAAAAATAG G T T GATATATAAAAG T T GT T GA

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 360 21 R. Suitably the functional version of MGF 360 21 R comprises the sequence of SEQ ID No. 124, 125, 126, 127, 128, 129, 130, 131, 132 or 133. Suitably the functional version of MGF 360 21 R comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 124, 125, 126, 127, 128, 129, 130, 131, 132 or 133. Suitably the functional version of MGF 360 21 R consists of the sequence of SEQ ID No. 124, 125, 126, 127, 128, 129, 130, 131, 132 or 133.

MGF 5051R gene sequences

SEQ ID No. 134 - Georgia 2007/1 MGF 505 1R (LR743116.1:28707-30302)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTAACCGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATACAGCAACATACCCTCATTC CCGTAAATGAAGCCCTGAGAACAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTCATATTTCACAAATGCCATATCATGCGGCAATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTACTTTAAACATAGATTAGAGGATG ATTTGCCCTTCACTCATTTACTT ATT GAAAAG GCAT GTAAAGAT CATAAT TAT GAAGT TATTAAAT GGATATAT GAAAAC CTACATAT CTACAATAT GATAGATAC CTTTGAATGTGCTATTGCCCATAAGGATCTACATCTATATTGTTTGGGGTATAGATTTAT ATATAACAGAATCGTACCCGATA AGTATCATCATTTAGATATTCGCATGCTTTCAAGCCTACAACTCCTACATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTA GAAACCTTAAAGTATGAT CATAAT AAAGATAATATAAATATTATTCTAACACAAGCTGCAACCTATAACCATAGAAAAAT TTTAATCTATTTCATTCCTCAATCAACCCACGCACAGATAGAACAATGTTTACTAGTGGC GATAAAAGCAAAATCTTCCAGGA AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAACCTCATCAAAAAAATAA GCCATTATGTTGCCACTTACAAT TCAACAAATATAATAGGCATTCTGAGTATGCGGCGGAAAAAGAAGATATATTTAGATATC ATATTGACAAAATTTGTAAAAAA AGCTATTTTTAATAAGTTTGTCGTTCGATGTATGGATACATTTTCTATAAACCCGGAAAG AATCCTTAAAATAGCCGCGCGAA TAAATAGGATGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGTTA GACTTAAATACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGATCGC TGTTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACCAATATCAACATC GTGAACAAGTATATCGGCAACCTGTTTGTTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATTTATTC T AAAC AATACAT G C C T T AG

SEQ ID No. 135 - China/2018/AnhuiXCGQ MGF 505 1R (MK128995.1:27736-29331)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTAACCGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATACAGCAACATACCCTCATTC CCGTAAATGAAGCCCTGAGAACAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTCATATTTCACAAATGCCATATCATGCGGCAATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTACTTTAAACATAGATTAGAGGATG ATTTGCCCTTCACTCATTTACTT ATT GAAAAG GCAT GTAAAGAT CATAAT TAT GAAGT TATTAAAT GGATATAT GAAAAC CTACATAT CTACAATAT GATAGATAC CTTTGAATGTGCTATTGCCCATAAGGATCTACATCTATATTGTTTGGGGTATAGATTTAT ATATAACAGAATCGTACCCGATA AGTATCATCATTTAGATATTCGCATGCTTTCAAGCCTACAACTCCTACATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTA GAAACCTTAAAGTATGAT CATAAT AAAGATAATATAAATATTATTCTAACACAAGCTGCAACCTATAACCATAGAAAAAT TTTAATCTATTTCATTCCTCAATCAACCCACGCACAGATAGAACAATGTTTACTAGTGGC GATAAAAGCAAAATCTTCCAGGA AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAACCTCATCAAAAAAATAA GCCATTATGTTGCCACTTACAAT TCAACAAATATAATAGGCATTCTGAGTATGCGGCGGAAAAAGAAGATATATTTAGATATC ATATTGACAAAATTTGTAAAAAA AGCTATTTTTAATAAGTTTGTCGTTCGATGTATGGATACATTTTCTATAAACCCGGAAAG AATCCTTAAAATAGCCGCGCGAA TAAATAGGATGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGTTA GACTTAAATACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGATCGC TGTTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACCAATATCAACATC GTGAACAAGTATATCGGCAACCTGTTTGTTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATTTATTC T AAAC AATACAT G C C T T AG SEQ ID No. 136 - Tengani 62 MGF 505 1R (AY261364.1 :22891 -24486)

ATGTTCTCTCTTCAGAACCTATGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATACAGCAACATAACCTCATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGAGTCTTT TATTAGCATGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAGTATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTAATATTTCACAAATGCCATATCATGCGGCAATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTCTTTTAAACATAGATTAAGGGATG ATTTGCCCTTCACTCATTTACTT ATT GAAAAG GCAT GTAAAGAT CATAAT TAT GAAGTAATTAAAT GGATATAT GAAAAC CTACATAT CTACAATAT GATAGATAC CTTTGAATGTGCTATTGCCCATAAGGATCTACGTCTATATTGTTTGGGGTATAGATTTAT ATATAACAGGATCGTACCCGATA AGTATCATCATTTAGATATTCGCATGCTTTCAAGCCTACAACTCCTGCATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTA GAAACCTTAAAGTATGAT CATAAT AAAGATAATATAAATATTATTCTAACACAAGCTGCAACCTATAACCATAGAAAAAT TTTAATCTATTTCATTCCTCAATCAACCCACGCACAGATAGAACAATGTTTACTAGTGGC GATAAAAACAAAATCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAATCTCATCAAAAAAATAA GCCATTATGTTGCCACTTACAAT TCAACAAATATAATAGGCATTCTGAGTATGCGGCGGAAAAAGAAGATATATTTAGATATC ATATTGACAAAATTTGTAAAAAA AGCTATTTTTAATAAATTTGTCGTTCGATGTATGGATACATTTTCTATAAACCCGGAAAG AATCCTTAAAATAGCCGCGCGAA TAAATAGGACGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGTTA GACTTAAATACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGATCGC TGTTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACCAATATCAACATC GTGAACAAGTATATCGGCAACCTGTTTGTTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TACAT G C C T T AG

SEQ ID No. 137 - Warmbaths MGF 505 1R (AY261365.1:27651-29246)

ATGTTCTCTCTCCAGAACCTATGTCGAAAAACTTTACCTGACTGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATACAGCAACGTACCCTCATTC CCGTAAATGAAGCCCTGAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGGGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGAGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTCATATTTCACAAATGCCATATAATCCGACGATG CTTTTTTAATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTACTTTAAACATAGATTAGAGGATG ATTTGCCCCTCACTCATTTACTT ATT GAAAAG GCAT GTGAAGAT CATAATTATGAAGTTATTAAAT GGATATAT GAAAACCTACATACCTACGATATAATGGAT AC CTTTGAATGTGCTATTGCCCATAAGGATCTACGTCTATATTGTTTGGGGTATACATTTAT ATATAACAGGATCGTACCCTATG AGTATCATCATTTAGATATTCTCATTCTTTCAAGCCTACAACTCCTGCATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTA GAAACCTTAAAGTATGAT CATAACAATGATAATTTAGATATTATTCTAACACAAGCTGCAACAT AT AACCATAGAAAAAT TTTAACCTATTTCATTCCTCAATTAACCTACGCACAAATAGAACAATGTTTGTTCATGGC GATAAAAAGAAAATCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAACCCTTTCCATCGAACTCATCAAAAAAATAA GCCAATATGTTGTCACTTACAAT TCAACAAATATAATAGGCATTCTGAGTATGAAGCGGAAAAAGAAGATATATTTAGATATC ATGTTGACAAAATATGTAAAATA CGCTATTTTTAATAAATATGTCGTTCGATGTATGGATAGATTTTCCATAAACCCGGAAAG AATCATCAAAATGGCCGCGCGAA TAAATAGGATGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGCTA GACTTAAACACCTTAAGCATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGATCGC TGCTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTATCCTAG ATACTATACAATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACCAATATCAACATC GTGAACAAGTATATTGGCGACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TACAT G CTTTAG

SEQ ID No. 138 - Mkuzi 1979 MGF 505 1R (AY261362.1:29425-31020)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTGATTGTAAACTTCCTGAA TTTTTTGACGATTATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGGGCAGGAAACAACTGTGTGCT TATACAGCAACATACCCTTATTC CCATAAATGAAGCCCTGAGAACAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCATGGGAGGGGAATCTT TACTATGCTATTATAGGGGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTAATATTTCACAAATGCCATATCATGCGGCGATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTATTTTAAATATAGGTTAGAGGATG ATTTACCCCTCACTCATTTACTT ATT GAAAAG GCAT GT GAAAAT CATAAT TAT GAAGT TAT TAGAT GGATATAT GAAAAC CTACATAT CTACAATAT GATAGATAC CTTTGAATGTGCTATTGCCCATAAGGATCTACGTCTATATTGTTTGGGGTATACATTTAT ATATAACAGGATCGTACCCAATA AGTATCATCATATAGATATTCTCATTCTTTCAAGCCTACAACTCCTGCATAAGGTGGCAG CCAAAGGATACTTAGACTTTATC CTA GAAACCTTAAAGTATGAT CATAACAATGATAATTTAGATATTATTCTAACACAAGCTGCAACAT AT AACCATAGAAAAAT TTTAACCTATTTTATTCCTCAATCAACCTACGCACAAATAGAACAATGTTTGATGGTGGC GATAAAAACAAAATCTTCCAAGA AAACCTTGAATTTACTATTGTCCCACCTAAACCTTTCCATCAAACTCATCAAAAAAATAA GCCAATATGTTGTCACTTACAAT TCAACAAATATAATAGGCATTCTGAGTATGAAGCGAAAAAAGAAGATATATTTAGATATC TTGTTGACAAAATTTGTAAAAAA TGCTATTTTTAATAAATTTGTCGTTCGATATATGGATACGTTTTCTATAAACCCGGAAAA AATCGTCAAAATGGCCGCGCGAA TAAATAAGATGATGTTAGTGAAAAAAATATCTGAACATATTTGGAAAAATCATGCGGCTA GACTTGAACACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGAGTAC TGCTATTACCATATGCAAGGGGA AGAAATTTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTCGACGT TTTTTATAATTGTTGTATCCTAG ATACGATACGATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACTAATATCAACATC GTGAACAAGTATATTGACAACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATTTATTC T AAACAT TATAT G C C T T AG

SEQ ID No. 139 - Warthog MGF 505 1R (AY261366.1:24387-25982)

ATGTTTTCTCTCCAGAACTTGTGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATACAGCAACATACCCTCATTC CCGTAAACGAAGCCCTGAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGAAACCTT TACTATGCTATTATAGGAGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACGATCCAGTCATATTTCACAAATGCCATATCATGCGGCGATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTATTTTAAATATAGGTTAGAGAATG ATTTGCCCCTCGCTCATTTACTT ATTAAAAAGGCATGTGAAGATCATAATTATGAAGTTATTAAATGGATATATGAAAACCTA CATATCTACAATATAATGGATAC CTTTGGATGTGCTATTGCCCATAAGGATCTACGTCTATATCGTTTGGGGTATACATTTAT ATATAACAGGATCGTACCCTATA AGTATCATTATTTAGATGTTCTCATTCTTTCAGGCCTACATCTCCTGTATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTAGAAACCTTAAAGTATGATCATAACAATGATAATTTAGATATTATTCTAACACAAGCT GCAACATATAACCATAGAAAAAT TTTAACCTATTACATTCCTCAATTAACCTATGCACAAATAGAACAATGTTTGTTCATGGC GATTAAAAAAAAATCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAAAGCTTTCCATCAAACTCATCAAAAAAATAA GCCAATATGTTGCCACTTACAAT TCAACAAATATAATAGGCATTCTGAATATGCGGCGGAAAAAGAAGATATATTTAGATATC ATATTGACAAAATTTGTAAAAAA AGCTATTTTTAATAAATTTGTCGTTCGATGTATGGATACATTTTCCATAAACCCGGAAAG AATCATCAAAATGGCCGCGCGAA TAAATAAGATGTTGTTAGTGAAAAAAATATCTGAACATGCTTGGAAAAATCATGCGGCTA GACTTAAACACCTTAAGCATGCG GTATACACGATGAAACATAAAGATGGGAAAAATAGACTCATGAACTTGATCTATGATCAC TACTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTCTCCTAG ATACTATACGATTTAAAAACCTTCTTTTAGATTGTTCACACATCATAGGTAAAAACGCTC ATGATGCTACTAATATCACTATC GTGAACAAGTATATTGGCAACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TACAT G C C T T AG

SEQ ID No. 140 - L60 MGF 505 1R (NC_044941.1 :22320-23915)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGATTATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGGGCAGGAAACAACTGTGTGCT TATACAACAACATACCCTCATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGGGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGTAACCGCTACAACTTAATTCGTAAATATGAT GATCAAATCAAGGACCATCATGA CATTCTGCCATTCATTGATGATCCAATCATATTTCACAAATGCCATATCATGCGGCGATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGTGTTCTTCTTTATTTTAAATATACATTAGAGGATG ATTTGCCCCTCGTTCATTTACTT ATTGAAAAGGCATGTGAAGATCATAATTATGAAGTTATTAAATGGATATATGAAAACCTA CATGTCTGCCATATAATAGATAC CTTTGACTGTGCTATTGCCCATAAAGATCTACGTTTATATTGTTTGGGGTATACATTTAT ATACAACAGGATTGTACCCTATA AGTATCATCATTTAGATATTCTCATACTTTCAAGCCTACAACTCCTACATAAGGTGGCGG CCAAAGGATACTTAGATTTTATC CTAGAAACCTTAAAGTATGATCATAATATAGATAATTTAGATGTTATTCTAACACAAGCT GCAACATATAACCATAGAAAAAT TTTAACCTATTTTATTCCTCAATCAACCTACGCACAAATAGAACAATGTTTGTTCGTGGC GATAAAAACAAAATCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAAACTCATCCAAAAAATCA GCCAATATGTTGCCACTTTCAAT TCAACAAATATAATAGGCATTCTGAGTATGAAGCGGAAAAAGAAGATATATTTGGATATC ATATTGACAAAATTTGTAAAAAA TGCTATTTTTAATAAATTTGTCGTTCGATGTATGGAGAGATTTTCTATAAACCCGGAAAG AATCGTCAAAATGGCTGCGCGTA TAAATAAGATGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGCTA GACTTAAACACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGAACAC TGCTATTACCATATGCAAGGGGA AGAAATTTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTCGACGT TTTTTATAATTGTTGTATCCTAG ATACGATACGATTCAAAAGCCTTCTTTTAGATTGTTCACATATCATAGGTAAAAACGCTC ATGATGCTACTAATATCAACATC GTGAACAAGTATATTGGCAACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TATAT G C C T T AG

SEQ ID No. 141 - Benin 97/1 MGF 505 1 R (NC_044956.1 :21971 -23566)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGATTATATATTACAACT

GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGGGCAGGAAACAACTGTGT GCTTATACAACAACATACCCTCATTC

CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGGGCC TTTTATTAGCGTGGGAGGGGAACCTT

TACTATGCTATTATAGGGGCTCTAGAGGGTAACCGCTACAACTTAATTCGTAAATAT GATGATCAAATCAAGGACCATCATGA

CATTCTGCCATTCATTGATGATCCAATCATATTTCACAAATGCCATATCATGCGGCG ATGCTTTTTTGATTGTATTTTATATC

AAGCTGTAAAATATAGTAAGTTTCGTGTTCTTCTTTATTTTAAATATACATTAGAGG ATGATTTGCCCCTCGTTCATTTACTT

ATTGAAAAGGCATGTGAAGATCATAATTATGAAGTTATTAAATGGATATATGAAAAC CTACATGTCTGCCATATAATAGATAC

CTTTGACTGTGCTATTGCCCATAAAGATCTACGTTTATATTGTTTGGGGTATACATT TATATACAACAGGATTGTACCCTATA

AGTATCATCATTTAGATATTCTCATACTTTCAAGCCTACAACTCCTACATAAGGTGG CGGCCAAAGGATACTTAGATTTTATC

CTAGAAACCTTAAAGTATGATCATAATATAGATAATTTAGATGTTATTCTAACACAA GCTGCAACATATAACCATAGAAAAAT

TTTAACCTATTTTATTCCTCAATCAACCTACGCACAAATAGAACAATGTTTGTTCGT GGCGATAAAAACAAAATCTTCCAAGA

AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAAACTCATCCAAAAAA TCAGCCAATATGTTGCCACTTTCAAT TCAACAAATATAATAGGCATTCTGAGTATGAAGCGGAAAAAGAAGATATATTTGGATATC ATATTGACAAAATTTGTAAAAAA TGCTATTTTTAATAAATTTGTCGTTCGATGTATGGAGAGATTTTCTATAAACCCGGAAAG AATCGTCAAAATGGCTGCGCGTA TAAATAAGATGATGTTAGTGAAAAAAATATCTGAACATGTTTGGAAAAATCATGCGGCTA GACTTAAACACCTTAAACATGCG GTACACACGATGAAGCATAAAGATGGGAAAAATAGACTCATGAACTTTATCTATGAACAC TGCTATTACCATATGCAAGGGGA AGAAATTTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTCGACGT TTTTTATAATTGTTGTATCCTAG ATACGAT ACGATT CAAAAGCCTTCTTTTA GATT GTTCACAT AT CATAGGTAAAAACGCTCATGATGCTACTAATATCAA CATC GTGAACAAGTATATTGGCAACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TATAT G C C T T AG

SEQ ID No. 142 - Pretorisuskop/96/4 MGF 505 1 R (AY261363.1:28449-30044)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TGTACAACAACATACCCTCATTC CCGTAAACGAAGCCCTGAGAATAGCAGCGTCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGAAACCTT TACTATGCTATTATAGGAGCTCTAGAGGGCAACCGCCACGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTCATTGACAATCCAGTCATATTTCACAAATGCCATATAATGCGGCGATG CTTTTTTGATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGCGTTCTTCTTTATTTTAAATATAGGTTAGAGAATG ATTTGCCCCTCGCTCATTTACTT GTTGAAAAGGCATGTGAAGATCATAATTATGAAGTTATTAAATGGTTATATGAAAACCTA CATATCTACAATATAATGGAGAC CTTTGAATGTGCTATTGCCCATAAGGATCTACGTCTATATCGTTTGGGGTATACATTTAT ATATAACAGGATCGTACCCTATA AGTATCATTATTTAGATGTTCTCATTCTTTCAGGCCTACATCTCCTGTATAAGGTGGCAG CCAAAGGATACTTAGATTTTATC CTAGAAACCTTAAAGTATGATCATAACAATGATAATTTAGATATTATTCTAACACAAGCT GTAACATATAACCATAGAAAAAT TTTAACCTATTACATTCCTCAATTAACCTATGCACAAATAGAACAATGTTTGTTCATGGC GATTAAAAAAAAATCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAAAGCTTTCCATCAAACTCATCAAAAAAATAA GCCAATATGTTGCCACTTACAAT TCAACAAATATAATAGGCATTCTGAATATGAAGCGGAAAAAGAAGATATATTTAGATATC ATATTGACAAAATTTGTAAAATA CGCTATTTTTAATAAATATGTCGTTCGTTGTATGGATACATTTTCCATAAACCCGGAAAG AATCATCAAAATGGCCGCGCGAA TAAATAAGATGTTGTTAGTGAAAAAAATATCTCAACATGCTTGGAAAAATCATGCGGCTA GACTTAAACACCTTAAGCATGCG GTATACACGATGAAACATAAAGATGGGAAAAATAGACTCATGAACTTGATCTATGATCAC TACTATTACCATATGCAAGGGGA AGAAATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCACCAAAGTTGTTTGACGT TTTTTATGATTGTTGTCTCCTAG ATACTATACGATTTAAAAGCCTTCTTTTAGATTGTTCACACATCATAGGTAAAAACGCTC ATGATGCTACTAATATCACTATC GTGAACAAGTATATTGGCAACCTGTTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTA CAGGACTATCCATCCATCTATTC T AAACAT TACAT G C C T T AG

SEQ ID No. 143 - Kenya 1950 MGF 505 1 R (AY261360.1 :31904-33496)

ATGTTTTCTCTCCAGAACTTATGTCGAAAAACCTTACCTGACCGTAAACTTCCTGAA TTTTTTGACGATTATGTATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACGATTCAACGAGCAGGAAACAACTGTGTACT TATCCAACAACATAACCTCATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCTTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGTAATCGCCACAACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTTATTGACGATCCAGTCATATTTCACAAATGTCATATGATGCGGCGATG CTTTTTTAATTGTATTTTGTATC AAGCTGTAAAATATAGTAAGTTTAGCGTTCTTCTATATTTTAAATATATATTAAAGGAGA ATTTGCCCCTCGTTCATTCACTT ATTGAAAAGGCGTGTGAAGATCATAATTATGAAGTTATTAAATGGATCTATGAAAACCTA CATATCTATGAAATAATGGATAC CTTTAAATGTGCTATTGCCCATAAAGATCTACATTTATATAGTTTAGGATATACATTTAT ATATAATAGGATCGTACCCTATA AGTATCATCATTTAGATATTCGCATACTTTCAAGGCTACAACTTCTACATAAGGTGACAG CCAAAGGATACTTGGACTTTATC CTAGAAACCTTAAAGTATGATCATAATAAAGATAATATAAATATTATTCTGACACAAGCT GCAACCTATAACCATAGAAACAT TTTAACCTATTTCATTCCTCAATCAACCTACGCGCAGATAGAACAATGCTTATTCGTGGC AATAAAAACAAACGCTTCCAAGA AAACCTTGAATTTACTATTATCTCACCTAAACCTTTCTATCAAACTCGTCAAAAAATTAA GCCAATATGTTGTTGCTTACAAG T CAACAAATAT CATAAGCATTCT GAGTAT GCAGCAAAAAAAGAAGATATATTTAGATAT CATTTT GACAAAGGTT GTAAAAAA CGCTATTTTTATTAAATTTGTCATTGGATGTATGGTTACATTTTCCATAAACCCAGAAAG AATTGTCAAAATGGCCGCGCGAA TAAAAAAGATGAAGTTAGTGAAAAATATATCTGAACATGTTTGGAAAAATCATGCGGCTA AACTTAAACACCTTAAGCATGCG GTACACACGATGAAGCATCAAGAAGGAAAAAATAGATTAATGAACTTTATCTATGATCAC TGTTATTACCACATGCAAGGAGA AGAGATCTTTAGTCTTGCAAGATTTTATGCAATCCATCATGCGCCAAAATTGTTCGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTTAAAAGCCTGCTTTTAGATTGTTCATATATCATAGCTAAAAACGCTC ATGATGCTAGTATTAACATCGTG AATAAGTATATTGGCAATCTATTTGCTATGGGGGTTCTTAGCAAAAAAGAAATCTTACAA GACTATCCATCCATCTATTCTAA G GAT TACAT G C T T TAG

SEQ ID No. 144 - Ken06.Bus MGF 505 1R (NC_044946.1 :25784-27376)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACCTTACCTGACTGTAAACTTCCTGAG TTTTTTGACGATTATGTATTACAACT GCTGGGCTTATACTGGGAAAATCATGGTACGATTCAACGAGCAGGAAACAACTGTGTACT TATCCAACAACATAACCTCATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAGGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGGAACCACCACAACTTAATTCGTAAATACGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTTATTGACGATCCAGTCATATTTCACAAATGTCATACAATGCGGCGATG CTTTTTTAATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTCAGCGTTCTTCTATACTTTAAATATATATTAAAGGAGA ATTTGCCCCTCGTCCACTCACTT ATT GAAAAG GC GTATAAATAT CATAAT TAT GAG GT TATTAAAT GGAT CTAT GAAAAC CTACATAT CTAT GATAT CATAAATAC TTTTAAATGTGCTATTGCCCATAAAGATCTACGTTTATATTGTTTAGGGTATACATTTGT ATATAATAGAATCGTACCCTATA AGTATTATCATTTAGATATTCGCATCCTTTTAAGGCTACAACTTCTACATAAGGTGACAG CCAAAGGATACTTGGATTTTATC CTAGAAACCTTAAAGTATGATCATAATACAAACAATATAGATATTATCCTAACACAGGCT GCAACCTATAACCATAGAAACAT TTTAACCTATTTCATTCCTCAATCAACCTACGCACAAATAGAACAATGTTTATTCGTGGC GATAAAAACAAATGCTTCCAAGA AAACCTTGAATTTACTATTATCTCACCTAAATCTTTCTATCAAACTCGTCAAAAAATTAA GCCAATATGTTGTCGCTTACAAG TCAACAAATATCATAAGCATTCTGAGTAGGCAGCAAAAAAAGAAGATATATTTAGATATC ATTTTGACAAAGGTTGTAAAAAA TGCTGTTTTTAATAAATTTGTCATTGGATGTATGGTTACATTTTCCATAAACCCAGAAAG AATTGTCAAAATGGCCGCGCGAA TAAAAAAGATGAAGTTAGTAAAAAATATATCTGAACATGTTTGGAAAAATCATGCGGTTA AACTTAAATATCTTAAGCATGCG GTACACACGATGAAGCATCAAGAAGGAAAAAATAGATTAATGAACTTTATCTATGATCAC TGTTATTACCACATGCAAGAAGA AGAGATCTTTAGCCTCGCAAGATTTTATGCAATCCATCATGCGCCAAAATTGTTCGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTTAAAAGCCTGCTTTTAGATTGTTCACATATCATAGTTAAAAACGCTC ATGATGCTAGTATTAACATCGTG AACAAGTATATTGGCAATCTATTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTACAA GACTATCCATCTATCTATTCTAA G GAT T ACAT G C T T TAG

SEQ ID No. 145 - Ken05/Tk1 MGF 505 1R (NC_044945.1:29657-31249)

ATGTTCTCTCTCCAAAACTTATGTCGAAAAACCTTACCTGACTGTAAACTTCCTGAA TTTTTTGACGATTATGTATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACGATTCAACGAGCAGGAAACAACTGTGTACT TATCCAACAACATAACCTCATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGGAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGTAACCGCCACAACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTCTGCCATTTATTGACGATCCAGTTATATTTCACAAATGTCATATAATGCGGCGATG CTTTTTTAATTGTATGTTGTATC AAGCTGTAAAATATAGTAAGTTTAGCGTTCTTCTATATTTTAAATATATATTAAAGGAAA ATTTGCCCCTCGTCCACTCACTC ATT GAAAAAGC GTATAAATAT CATAAT TAT GAG GT TATTAAAT GGAT CTAT GAAAAT CTACATAT CTAT GATAT CATAAATAC TTTTAAATATGCTATTGCCCATAAAGATCTACGTTTATATTGTTTAGGGTATACATTTGT ATATAATAGGATCGTACCCTATA AGTATTATCATTTAGATATTCGCATCCTTTTAAGGCTACAACTTCTACATAAGGTGACAG CCAAAGGATACTTGGATTTTATC CTA GAAACCTTAAAGTATGAT CATAAT ACAAACAATATAGATATTATCCTAACACAGGCTGCAACCTATAACCATAGAAACAT TTTAACCTATTTCATTCCTCAATCAACCTACGCACAAATAGAACAATGCTTATTCGTGGC AATAAAAACAAACGCTTCCAAAA AAACCTTGAATTTACTATTATCTCACCTAAACCTTTCTATCAAACTCGTCAAAAAATTAA GCCAATATGTTGTCGCTTACAAG T CAACAAATAT CATAAGCATTCT GAGTAT GCAGCAAAAAAAGAAGATATATTTAGATAT CATTTT GACAAAGGTT GTAAAAAA CGCTGTTTTTATTAAATTTGTCATTGGATGTATGGTTACATTTTCCATAAACCCAGAAAG AATTGTCAAAATGGCCGCGCGAA TAAAAAAGATGAAGTTAGTAAAAAATATATCTGAACATGTTTGGAAAAATCATGCGGCTA AACTTAAACATCTTAAGCATGCG GTACACACGATGAAGCATCAAGAAGGAAAAAATAGACTCATGAACTTTATCTATGATCAC TGCTATTACCACATGCAAGGAGA AGAGATCTTTAGTCTTGCAAGATTTTATGCAATCCATCATGCGCCAAAATTGTTCGACGT TTTTTATGATTGTTGTATCCTAG ATACGATACGATTTAAAAGCCTGCTTTTAGATTGTTCACATATTATAGCTAAAAACGCTC ATGATGCTAGTATTAACATCGTG AATAAGTATATTGGCAATCTATTTGCTATGGGAGTTCTTAGCAAAAAAGAAATCTTACAA GACTATCCATCCATCTATTCTAA ATAT GACAT AC T T TAG

SEQ ID No. 146 - Malawi Lil-20/1 (1983) MGF 505 1R (AY261361.1:26041 -27633)

ATGTTCTCTCTCCAGAACTTATGTCGAAAAACATTACCTGACTGTAAACTTCCTGAA TTTTTTGACGAATATATATTACAACT GCTGGGATTATACTGGGAAAACCATGGAACTATTCAACGAGCAGGAAACAACTGTGTGCT TATCCAGCAACATAACCTTATTC CCGTAAATGAAGCCCTAAGAATAGCAGCATCTGAAGAAAATTATGAGATCGTGAGCCTTT TATTAGCGTGGGAGGGAAACCTT TACTATGCTATTATAGGGGCTCTAGAGGGCAACCGCCCCGACTTAATTCGTAAATATGAT GACCAAATCAAGGACCATCATGA AATTTTGCCATTCATCGACGATCCAATCATATTTCACAAATGTCACATAATGCGGCGATG CTTTTTTAATTGTATTTTATATC AAGCTGTAAAATATAGTAAGTTTCGTGTTCTTCTTTATTTTAAACATAGGTTAGGGGATG ATTTGCCCCTCACTCATTTACTT ATT GAAAAGGCATGTGAAGAT CATAATTATGAAGTTATTAAAT GGAT CTAT GAAAACCTACATAGCTACAATATAAT GGAT AC CTTTGAATGTGCTATTGCCCATAAGGATCTACGTCTATATTGTTTGGGGTATACATTTAT ATATAATAGGATCGTACCCTATA AGTATCATCATTTAGATATTTGCATACTTTCAAGCCTACAACTCCTGCATAAGGTGGCAG CCAAAGGATACTTGGATTTTATC CTA GAAACCTTAAAGTATGAT CATAACATAAATAATATAGATATTATTCTAACACAAGCTGCAACCTATAACCATAGAAAA AT TTTAACCTATTTCATTCCTCAATTAACCTACGCACAGATAGAACAATGTTTACTAGTGGC GATAAAAACAAAAGCTTCCAAGA AAACCTTGAACTTACTACTGTCTCACCTAAACCTTTCCATCAAACTCATCAAAAAAATAA GCCAATATGTTGTCACTTACAAT T CAACAAAT AT CATAAGCATTCT GAGTAT GCGGCGGAAAAA GAAGAT AT ATTTAGAT AT CATTTT GACAGAGTTT GTAAAAAA CGCTATTTTTAATAAATTTGTCGTTCGATGTATGGATACATTTTCCATAAACCCGGAAAG AATTGTCAAAATGGCCGCACGAA TAAATAGGATGATGTTAGTGAAAAATATATCTGAACGTGTTTGGAAAAATCATGCGGTTA AACTTAAACACCTTAAGCATGCG GTACATACGATGAAGCATCAAGAAGGAAAAAATAGACTCATGAACTTTATCTATGATCAC TGCTATTACCACATGCAAGGGGA AGAGATCTTTGGCCTCGCAAGATTTTATGCAATCCATCATGCACCCAAGTTGTTTGACGT TTTTTATGATTGCTGCATGCTAG ATGCTACACGATTTAAAAGCCTGCTTTTAGATTGTCCACATATCATAGGTAAAAACGCTT ATGATGCTGGTATCAACCTCGTG AACAAATATATTGGCAACCTATTTGCTATGGGGGTTCTTAGCAAAAAAGAAATCTTACAG GACTATCCATCTATCTATTCTAA ACAT GATAT GT TT TAG

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 505 1R. Suitably the functional version of MGF 505 1R comprises the sequence of SEQ ID No. 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145 or 146. Suitably the functional version of MGF 505 1R comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145 or 146. Suitably the functional version of MGF 505 1 R consists of the sequence of SEQ ID No. 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145 or 146.

MGF 5052R gene sequences

SEQ ID No. 147 - Georgia 2007/1 MGF 5052R (NC_044959.1:33119-34699)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCATAGGAGACGACCACATACTCATACG ACGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCGTCATAGGAGCCTTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATTTTATCTT ACCATTGATTCAAGACGCGAATACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAAAACATG CTACAAAATACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATGAGAG CGTATCTTCACGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAAACCATACAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAACATCTCAAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGCAAC ATAGATACCGTCCTGACCCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAACTTTTGTTGCTGGCCGTGCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTACCGCGCTATGTGATAGAGTACGAGTCCACCTTGGTGA TAAAGATTTTATTAAAAAAAAGA GTGAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGTCTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGGGAAAAAGTCATGTTTTATTTA GCCAAGCTCTATGTTGCTCAAAA CGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TATTAGACTGTTTAGAAATTATTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTGTTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAAGTAATT CATTATAAAACAATACAAT G TTAA

SEQ ID No. 148 - China/2018/AnhuiXCGQ MGF 5052R

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCATAGGAGACGACCACATACTCATACG ACGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCGTCATAGGAGCCTTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATTTTATCTT ACCATTGATTCAAGACGCGAATACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAAAACATG CTACAAAATACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATGAGAG CGTATCTTCACGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAAACCATACAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAACATCTCAAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGCAAC ATAGATACCGTCCTGACCCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAACTTTTGTTGCTGGCCGTGCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTACCGCGCTATGTGATAGAGTACGAGTCCACCTTGGTGA TAAAGATTTTATTAAAAAAAAGA GTGAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGTCTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGGGAAAAAGTCATGTTTTATTTA GCCAAGCTCTATGTTGCTCAAAA CGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TATTAGACTGTTTAGAAATTATTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTGTTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAAGTAATT CATTATAAAACAATACAAT G TTAA

SEQ ID No. 149 - Mkuzi 1979 MGF 5052R (AY261362.1:34826-36406)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG

ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCATAGGAGACGACCACATACTCAT ACGACGGGATCTCATCCTTTCCACCA

ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGT TACTGTGGAAGGGAAATCTTCATTAC

GCCGTCATAGGAGCCTTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAAC CAAATCGGCGACTTTCATTTTATCTT

ACCATTGATTCAAGACGCGAATACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTG TGGTGTTTCATGTCTGCTAAAACATG

CTACAAAATACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATGA GAGCGTATCTTCACGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAGACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAACATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGCAAC ATAGATACCGTCCTGACCCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAACTTTTGTTGCTGGCTGTTCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTATGAGTCCACCTTGGTGA TAAAGATTTTGTTAAAAAAAAGA GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACAAAAGTG AGGACGATCATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGTTTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGGGAAAAAGTCATGTTTCATTTA GCCAAGCTCTATGTTGCTCAAAA TGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TATTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTATTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAAGTAATT CATTATAAAACAATACAAT G TTAA

SEQ ID No. 150 - L60 MGF 505 2R (NC_044941.1 :27701-29281)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCATAGGAGACGACCACATACTCATACG ACGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCGTCATAGGAGCCCTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATTTTATCTT ACCATTGATTCAAGACGCGAATACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAAAACATG CTACAAAATACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATGAGAG CGTATCTTCACGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAGACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAACATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGCAAC ATAGATACCGTCCTGACCCAAGCCGTAAAGTACAACCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAGCTTTTGTTGCTGGCTGTTCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTATGAGTCCACCTTGGTGA TAAAGATTTTGTTAAAAAAAAGA GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGTCTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGGGAAAAAGTCATGTTTCATTTA GCCAAGCTCTATGTTGCTCAAAA TGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TATTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTATTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAAGTAATT CATTATAAAACAATACAAT G TTAA

SEQ ID No. 151 - Benin 97/1 MGF 5052R (NC_044956.1 :27352-28932)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCATAGGAGACGACCACATACTCATACG ACGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCGTCATAGGAGCCCTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATTTTATCTT ACCATTGATTCAAGACGCGAATACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAAAACATG CTACAAAATACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATGAGAG CGTATCTTCACGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAGACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAACATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGCAAC ATAGATACCGTCCTGACCCAAGCCGTAAAGTACAACCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAGCTTTTGTTGCTGGCTGTTCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTATGAGTCCACCTTGGTGA TAAAGATTTTGTTAAAAAAAAGA GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGTCTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGGGAAAAAGTCATGTTTCATTTA GCCAAGCTCTATGTTGCTCAAAA TGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TATTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTATTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAAGTAATT CATTATAAAACAATACAAT G TTAA

SEQ ID No. 152 - Tengani 62 MGF 5052R (AY261364.1 :28261 -29830 )

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG

ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCGTAGGAGACGACCACATACTCAT ACGGCGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCGTCATAGGAGCCTTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATCTTATCTT ACCATTGATTCAAGACGCGAAAACGTTTGAAAAATGCCATGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAAAACATG CTACAAAATACAACATGCTCCCTGTTCTCCAAAAATACCAAGAAGAGCTGTCCATGAGAG CATATCTTTGCGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAAACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACTATGTACTCCTTAGGATATATTTTCCTTTT TGATAGAGGGAACACCGAAGCTA CGTTGCTAACGCAATATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGTAAC ATAGATATTGTCCTGACCCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAGCTTTTGTTGCTGGCCGTGCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCACATTTAAACTACT CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCCACCTTGGTGA TAAAGATTTTATTAAAAAAAAGA GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTT GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTATGGGAGG ATGATCTAGAACGACTTACTCGTCTTAAAGATATGGTATACACCGTAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATACACGGCATATACAAAAACTTATTATACGGCGAAAAAGAAAAGGTCATGTTTCATTTA GCCAAGCTCTATGTTGCTCAAAA CGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TGTTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTATAGTATTCTGGAAATCTTAG AAAAACATATGATTTCCCTATTT ACTAT GAAAGT TAT GACT GAAGAAGAAAAAAAC CTAT GTTTAGAAATAT TATATAAG TAA

SEQ ID No. 153 - Pretorisuskop/96/4 MGF 5052R (AY261363.1:33795-35374)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGATTAGG ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCGTAGGAGACGACCACATCCTCATACG GCGGGATCTCATCCTTTCCACCA ACGAGGCCTTAAGAATGGCGGGAGAAGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAC GCCATCATAGGAGCCTTGCAGGGTGATCAATATGACCTGATCCATAAGTATGAAAACCAA ATCGGCGACTTTCATCTTATCTT ACCATTGATTCAAGATGCGAAAACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTGTGG TGTTTCATGTCTGCTAGAACATG CTACAAAATACAACATGCTCCCTATTCTCCAAACATACCAAGAAGAGCTGTCTATGAGAG CATATCTTCGCGAAACCCTATTT GAACTAGCGTGCCTGTGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAAACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTGACGATGTATTCCTTAGGATATATTCTTCTTTT TGATAGAGAGAACACCGAAGCTA CGTTGTTAACACAACATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCTACTTTGTGCTAG AAACGTTAAAATACGGCGGTAAC ATAGATATCGTCCTGACTCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAACA TATTGAAAAGCTTTTGTTGCTGGCCGTGCAGGAAAAGGCTTCTAAAAAAACATTGAACTT ACTGTTGTCCCATCTAAACTACT CCGTGAAACGTATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCCACCTTGGTGA TAAAGATTTTATTAAAAAAAAGA GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAAGTG AGGACGATCATGGATGAGCTTTT GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACGGATATCGTAAT CCATACTTCTTATGTTTGGGAGG ATGATCTAGAACGACTTACTCGTCTTAAAAATATGGTATACACCATAAAGTACGAACATG GGAAAAAAATGTTAATTAAAGTC ATGCACGGCATATACAAAAACTTATTATACGACGAAAGAGAAAAGGTCATGTTTCATTTA GCCAAGCTCTATGTTGCTCAAAA CGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACGGTT TAAACCGCGGTTTAAGCAACTAA TGTTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTATAGTATCCTGGAAATCTTAG AAAAACATATTATTTCCCTATTT ACTAT GAAAGT TAT GACT GAAGAGGAAAAAAAC CTAT GTTTAGAAATAT TATATAAG TAA

SEQ ID No. 154 - Warmbaths MGF 505 2R (AY261365.1:33029-34597)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTATTACAACGATTAGG

ACTGTATTGGAGATGTCACGGCTCCCTTCAACGCGTAGGAGACGACCACATACTCAT ACGGCGGGATCTCATCCTTTCCACCA

ACGAGGCCTTAAGAATGGCGGGAGAAGAAGGAAACAATGAAGTAGTAAAGCTCTTGT TACTGTGGAAGGGAAATCTTCATTAC

GCCATCATAGGAGCCTTGCAGGGTGATCAATATGACCTAATCCATAAGTATGAAAAC CAAATCGGCGACTTTCATCTTATCTT

ACCATTGATTCAAGATGCGAAAACGTTTGAAAAATGCCACGCTTTAGAACGTTTTTG TGGTGTTTCATGTCTGCTAAAACATG

CTACAAAATACAACATGCTCCCTATTCTCCAAACATACCAAGAAGAGCTGTCTATGA GAGTATATCTTCGCGAAACCCTATTT

GAACTAGCATGCCTATGGCAGAGGTATGATGTCCTTAAATGGATAGAGCAAACCATG CATGTTTACGACCTAAAGGTTATGTT

TAATATTGCCATCTCCAAGAGAGATCTGACTATGTATTCCTTAGGATATATTCTTCT TTTTGATAGAGAGAACACCGAAGCTA

CGTTGCTAACGCAACATCTCGAGAAAACAGCGGCCAAGGGGCTCCTCCACTTTGTGC TAGAAACGTTAAAATACGGCGGTAAC

ATAGATATCGTCCTGTCCCAAGCCGTAAAGTACAATCATAGAAAACTTTTAGATTAT TTTCTGCGTCAACTACCTCGTAAAAA

TATTGAAAAACTTTTGTTGCTGGCTGTTCAGGAAAAGGCTTCTAAAAAAACATTGAA CTTACTGTTGTCCCATTTAAACTACT

CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCCACCTTGG TGATAAAGATTTTATTAAAAAAAAGA

GTAAACCTGATAGATGCCATGTTGGAAAAGATGGTAAGATATTTTTCTGCGACGAAA GTGAGGACGATCATGGATGAGCTTTC

GATTAGTCCGGAAAGAGTCATTAAGATGGCTATACAGAAAATGAGAACAGATATCGT AATCCATACTTCTTATGTTTGGGAGG

ATGATCTAGAACGACTTACTCGTCTTAAAAATATGGTATACGCCATAAAGTATGAAC ATGGGAAAAAAATGTTAATGAAAGTC

ATGCACGGCATATACAAAAACTTATTATACGGCGAAAGAGAAAAGGTCATGTTTCAT TTAGCCAAGCTCTATGTTGCTCAAAA

CGCGGCCACCCAATTCAGAGACATTTGTAAGGACTGTTACAAACTGGATGTGGCACG GTTTAAGCAACTAACGTTAAACTGTT

TAGAAATTATTACTAAAAAATCTTGCTATAGTATCCTGGAAATCCTAGAAAAACATA TTATTTCCCTATTTACTATGAAAGTT

ATGACTGAAGAAGAAAAAAACCTATGTTTAGAAATATTATATAAAGTAATTCATTAT AATACAATACAATGTTAA

SEQ ID No. 155 - Malawi Lil-20/1 (1983) MGF 5052R (AY261361.1 :31541 -33121) ATGTTTTCCCTTCAAGATCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTTGGC GAGCATGTACTACAACAGTTAGG ACTGTACTGGAAACGTCACGGCTCTCTTCAACGCATAGGAGACGACCACATACTCATACG GCGGGACCTCATCCTTTCCACCA ACGAGGCCTTAAAAATGGCGGGAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGGAGGGAAACCTTCATTAT G C CAT CATAG G G G C C T T ACAG G G T GAT CAAT AT GAT C T GAT C CAT AAGT AT GAAAAC CAAAT C GAAGAC TAT CAT CATAT C T T GCCATTGATTCAAGATGCGAAAACGTTTGAAAAATGCCACGCCTTAGAACGTTTTTGTGA TGTTCCATGTCTGCTAGAACATG CTACAAAACACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTCTATAAGAG TGTATCTTCGCGAAACCCTATTC GAACTAGCATGCCTATGGCAGAGGTATGATGTTCTTAAATGGATAGAGCAAACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTAAGCATGTACTCCTTAGGATATGTTCTCCTTTT TGATAGAGGAAACATCGAAGCTA CGTTCCTAACGCAACATCTCGAGAAGACAGCGGCCAAAGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGTAAC TTAAATATCGTCCTGTCCCAAGCCGTAAAATACAATCATAGAAAACTTTTGGATTATTTT CTGCGTCAACTACCTCGTAAAAA TATTGAAAAACTTTTGTTGCTGGCCGTGCAGGAAAAGGCTTCTAAAAAAACATTGAACCT ACTGTTGTCACACTTAAACTACT CCGTGAAACACATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCTACCTTGGTGA TAAAACTTTTATTGAAAAAAAGA GTAAACCTGATAGACGCCGTGTTGGAAAAGAATGTAAGATATTTTTCTGCGATTAAAGTG AGGACTATTATGGATGAGCTTTC GATTAGTCCGGAAAGAGTCATTAAGATGGCCATACAGAAAATGAGAACGGATATTGTGAT TCAGACTTCTTATATTTGGGAGG ATGATCTAGAAAGACTTATTCGTCTTAAAAATATGGTATACACCATAAAGTATGAACATG GGAAAAAAATGTTAATTAAAGTT ATTCACGGCATATACAAAAACTTATTATACGGCGAAAAAGAAAAAGTCATGTTTCATTTA GCCAAACTCTATGTTGCTCAAAA CGCGGCCACCCAATTCAGGGACATTTGTAAGGACTGTTGCAAACTGGATGTGGCGCGGTT TAAACCGCGGTTTAAACAACTAA TTTTAGACTGTTTAGAAATGGTTACTAAAAAATCTTGCTTTAGTATTATAGAAATCTTAG AAAACTATATTATTTCCCTATTT GTGATGAAAGTCATCACTGAAGAAGAAAAAAACCTATGTTTAGAACTATTATATAAAGTA ATTAGTTACAAAACGATATAATG TTAA

SEQ ID No. 156 - Ken05/Tk1 MGF 5052R (NC_044945.1:35094-36674)

ATGTTTTCCCTTCAAGACCATTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACGGTTAGG

ACTGTACTGGGGACGTCACGGCTCTCTTCAACGAATCGGGGACGATCACATACTCAT ACGGCGGGACCTCATCCTTTCCACCA

ACGAGGCCTTAAAAATGGCGGGAGAAGAAGGAAACAATGAAGTAGTAAAGCTCTTGT TACTGTGGGAGGGAAATCTTCATTAT

GCTATCATAGGGGCTTTACAGGGTGATCAATATGACCTAATCCATAAGTATGAAAAC CAAATCGAAGACTATCATCATATCTT

ACCATTGATTCAAGATGCGGAAACGTTTGAAAAATGCCACGCCTTAGAACGTTTTTG TGATGTTCCATGTCTGCTAGAACATG

CTACAAAACACAACATGCTCCCTATTCTCCAAAAATATCAAGAAGAGTTGTCTATAA GAGTGTATCTACGCGAAACCCTATTC

GAACTAGCATGCCTATGGCAGAGGTATGATATTCTTAAATGGATAGAGCAAACCATG CATGTTTACGATCTAAAAATTATATT

TAATATTGCCATCTCCAAGAGGGATCTAAGCATGTACTCCTTAGGATATGTTCTCCT TTTTGATAGAGGGAACACCGAAGCTA

CCTTGTTAACGCAACACCTCGAGAAGACAGCGGCCAAGGGGCTCCTCCACTTTGTGC TAGAAACGTTAAAATACGGCGGTAAC

TTAAATATCGTCCTGTTCCAAGCCGTAAAATACAATCATAGAAAACTTTTAGATTAT TTTCTGCGTCAACTACCCCGTAAAAA

TATTGAAAAACTTTTGTTGCTGGCTGTGCAGGAAAAAGCTTCTAAGAAAACATTGAA CTTACTGTTGTCACATTTAAACTACT

CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCTACCTTGG TGATAAAGATTTTATTAAAAAAAAGA

GTAAACCTAATAGACGCCGTGTTGGAAAAGACTGTAAGATATTTTTCTGAGACGAAA GTAAAGACTATTATGGATGAGCTTTC

GATTAATCCGGAAAAAGTCATTAAGATGGCCATACAGAAAATGAGAACGGATATTGT GATCCAAACTTCTTATATTTGGGAGG

ATGATCTAGAAAGACTTATTCGTCTTAAAAATATGGTATACACCATAAAGTATGAAC ATGGGAAAAAAATGTTAATGAAAGTT

ATTCACGGCATATACAAAAACTTATTACACGATGAAAAAGAAAAAGTCATGTTTCAT TTAGCCAAGTTCTATATTGCTCAAAA

CGCGGCCACCCAATTCAGAGACATTTGTAAAGACTGTTGCAAACTGGATGTGGCGCG GTTTAAACCGCGGTTTAAACAACTAA

TTTTAGACTGTTTAGAAATTGTTACTAAAAAATCTTGCTTTAGTATTATAGAAATTT TAGAAAACCATATTATTTCCCTATTT

ATGATGAAAGTTATCACTGAAGATGAAAAAAACCTAGGTTTAGAATTATTATATAAA GTAATTAGTTACAAAATGATATCATA

TTAA

SEQ ID No. 157 - Kenya 1950 MGF 5052R (AY261360.1 :37419-38999)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCACGTACTACAACAATTGGG GCTGTACTGGGAAAAACACGGCTCTCTTCAACGAATAGGGAACGATCACATACTCATACG GCGGGATCTCATCCTTTCTATCA ACGAGGCCTTAAAAATAGCGGCAGAGGAAGGAAACAATGAAGTAGTAAAGCTTTTGTTAC TATGGAAGGGAAATCTTCATTAT GCCAT CATAGGAGCTTT GGAGGGTGAC CAATATGACCTGATCTATACGTAT GAAAAC CAAATTGAAGACTATCATCATATCTT GCCATTGATTCAAGATGCGAAAACGTTTGAAAAATGCCACGCCTTGGAACGTTTTTGTGA TGTTCCATGCCTGCTAGAGCATG CTATAAAACACAACATGCTCCCTATTCTCCAAAAATATCAAGAAGAACTGTTTATAAGAG TGTATCTCCGCGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATATTCTTAAATGGATAGAGCAAACCATGCAT GTTTACGATCTAAAAATTATATT TAATATTGCCATCTCCAAGAGGGATCTAAGCATGTACTCCTTAGGATATGTTCTCCTTTT TGATAGAGGGAACACCGAAGCTA CCTTGTTAACGCAACACCTCGAGAAGACAGCGGCCAAGGGGCTCCTCCACTTTGTGCTAG AAACGTTAAAATACGGCGGTAAC ATAAACATCGTCCTATCCCAAGCCGTAAAATACAATCATAGGAAACTTTTAGATTATTTT CTGCGTCAACTACCTCGTAAAAA TATTGAAAAACTTTTGTTGTTAGCCGTACAGGAAAAGGCTTCTAAGAAAACATTGAACTT ACTGTTGTCTTATTTAAACTACT CCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGTACGAGTCCACACTGGTGA TAAGGATTTTATTAAAAAAAAGA ATAAACCTAATAGACGCCGTGTTGGAAAAGACTGTAAGATATTTTTCTGAGACGAAAGTA AAGACTATTATGGATGAGCTTTC GATTAATCCGGAAAAAGTTATTAAGATGGCCATACAGAAAATGAGAACGGATATTGTGAT CCAAACTTCTTATATTTGGGAGG ATGATCTAGAAAGACTTATTCGTCTTAAAAATATGGTATACACCATAAAGTATGAACATG GGAAAAAAATGTTAATGAAAGTT ATTCACGGCATATACAAAAACTTATTACACGATGAAAAAGAAAAAGTCATGTTTCATTTA GCCAAGTTCTATATTGCTCAAAA CGCGGCCACCCAATTCAGAGACATTTGTAAAGACTGTTGCAAACTGGATGTGGCGCGGTT TAAACCGCGGTTTAAACAACTAA TTTTAGACTGTTTAGATATTATTACTAAAAAAACGTGCCTCAATATTATGGAAATCTTAG AAAATCATATTATTTCGCTATTT GCTATGAAAATGATGACTGAAGATGAAAAAAACCTAGGTTTAGAATTATTATATAAAGTA ATTAGTTACAAAATGATATCATA TTAA SEQ ID No. 158 - Ken06.Bus MGF 5052R (NC_044946.1:31225-32799)

ATGTTTTCCCTTCAAGACCTTTGCCGAAAGCATCTTTTTATTCTTCCCGATGTTTTT GGCGAGCATGTACTACAACAATTGGG GCTGTACTGGGAAAAACACGGCTCTCTTCAACGAATAGGGAACGATCACATACTCATACG GCGGGATCTCATCCTTTCTATCA ACGAGGCCTTAAAAATAGCGGCAGAGGAAGGAAACAATGAAGTAGTAAAGCTCTTGTTAC TGTGGAAGGGAAATCTTCATTAT G C CAT CATAG GAG C C T T G CAG G G T GAC CAAT AT GAC C T CAT C CAT AC GT AC GAAAAC CAAAT C GAAGAC TAT CAT CATAT C T T GCCATTGATTCAAGATGCGAAAACGTTTGAAAAATGCCACGCCTTGGAACGTTTTTGTGA TGTTCCATGCCTACTAGAACATG CTACAAAACACAACATGCTCCCTATTCTCCAAAAATACCAAGAAGAGCTGTTTATAAGAG TGTATCTCCGCGAAACCCTATTT GAACTAGCATGCCTATGGCAGAGGTATGATATCCTTAAATGGATAGAGCAAACCATGCAT GTTTACGACCTAAAGATTATGTT TAATATTGCCATCTCCAAGAGGGATCTAAGCATGTACTCTTTAGAATATATTCTCTTTTT TAATAGAGGGAACACCGATGTTG CGTTAGCAACGTTGCTAACGCAACATCTCGAGAAGACAGCGGCCAAGGGGCTCCTCCACT TTGTGCTAGAAACGTTAAAATAC GGCGGTAACATAAACATCGTCCTCTCCCAAGCCGTAAAATACAATCATAGAAAACTTTTA GATTATTTTCTGCGTCAACTACC TCGTAAAAATATTGAAAAACTTTTGTTGCTGGCCGTACAGGAAAGGGCTTCTAAGAAAAC ATTGAACTTACTGTTGTCTTATT TAAACTACTCCGTGAAACGCATCAAAAAACTGCTGCGCTATGTGATAGAGCATGAGTCCA CACTGGTGATAAGGATTTTATTA AAAAAAAGAATAAACCTAATAGACGCCGTGTTGGAAAAGACTGTAAGATATTTTTCTATG ACTAAAGTGAGGACGATCATGGA TGAGCTTTCGATTAATCCGGAAAAAGTCATTAAAATGGCCGTGCAGAAAATGAGAACGGA TATCGTGATCCATACTTCTTATG TTTGGGAGGATGATCTAGAAAGACTTATTCGTCTTAAAAATATGCTATACACTATAAAGT ATGAGCATGGAAAAAAAATGCTA ATTAAAGTCATTCACGGCATATACAAAAACTTATACGGCGAAAAAGAAAAAGTCATGTTT AATTTGGCCAAGTTCTATGCTGC TCAAAACGCGGCCACCCAATTCAAAGACACTTGTAAAGACTGTTGCAAACTGGATGTGGC GCGGTTTAAACAACTAATTTTAG ACTGTTTAGACATTATTACTAAAAAAACGTGCCTCAGTATCATGGAAATCTTAGAAAATC ATATTATTTCGCTATTTGCTATG AAAAT GAT GAC G GAG GAT G AAAAAAAC T T AG GT T T AG AAAT AT TATATAAAGT AAT T AG T T AC AAAAT GAT AT CATAT T AA

In an embodiment the attenuated ASFV of the invention comprises a functional version of MGF 505 2R. Suitably the functional version of MGF 505 2R comprises the sequence of SEQ ID No. 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157 or 158. Suitably the functional version of MGF 5052R comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157 or 158. Suitably the functional version of MGF 505 2R consists of the sequence of SEQ ID No. 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157 or 158. MGF 5056R gene sequences

SEQ ID No. 257 - Benin 97/1 MGF 5056R

ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACACTTTCTTCCTTCCAAATGATTTT AGCAAGCATACTCTACAACGGCTGGG GTTGTATTGGAAAGAGCATGGATCCGTCCATCGAATAGAAAAGGACAGCATAATGATACA GAATGAATTGGTTCTTTCTATCA ATGATGCTTTACAGCTTGCAGGAGAGGAGGGGGACACAGATGTGGTACAGCTCTTGTTAC TATGGGAGGGAAATCTGCATTAT GCCATCATAGGAGCTTTGAAGACTGAGAATTATAATCTAGTATGTGAGTACCATAGCCAA ATTCAGGACTGGCATATTCTCCT ACCCTTGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGGATGTGA CCTTATATGCCTTCTCCAACATG CTGTAAAATGTGATATGCTTTCTATTCTTGTTAAATATAAGGAGGATTTACTAAATGTAA GGATTAGGCATCGTACCCAATCC CTGTTTGTTTTGGCATGCGAAAATCGGAGATTTGAGATTATTGAATGGATAGGTCAAAAT CTGTCAATTCCTGAACCTGAGGC CATTTTTAGCATTGCTATTGTTACAAAAGATGTAGAACTGTTTTCCTTAGGATATAAAAT TATTTTTGATTACATGCAAAGAC AAGGAATTTTTCAATTAACCAATGTAGTTCGCATGCTTCTGCTAAATCGTCATATTGGTA TGGCAATAGAAAAAGGACTTTTA CCTTTTATTCTGGAAACTTTAAAATATGGTGGTAGTGTAAAAAGAGCTTTATCTTATGCA GTAATAGATAATAAAAGAAAAAT TATAGACTATCTTGTACGCCATGAAAATATACCCCGAGGAACTATTGAAAGACTTTTGCA TCTAGCTGTGAAAAAACAATCTT CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTAAAA AGCTGGTAGAGCATGTAGTAGAT CACAAATCCACTCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATCTAGTGGATGCT GTTTTAACAAGACTTGTAAAACA TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAAATT CATTAAAATAGCTGTGCGGGAAA AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAAGAA TTACTTATCTCAAACAGATAGTG CACACCATAAAATATGAAAGTGGAAGGCGGTTTTTGATAGACATCATTCACAGCATTTAC CAAAGTTACTCACTAAAACACGA AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAATCACCCATTTTAAAGA TCTCTGCAAATATCTTTGGCTGA ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGCTTGGAAATTGCTGATGAGAAGG AGTTTCCTGATATTAAAAATATT GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATCATG CAAGCTTATGCTTTAGAGTATGA CATGTATTAA

SEQ ID No. 258 - L60 MGF 5056R ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACACTTTCTTCCTTCCAAATGATTTTAGC AAGCATACTCTACAACGGCTGGG

GTTGTATTGGAAAGAGCATGGATCCGTCCATCGAATAGAAAAGGACAGCATAATGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACAGCTTGCAGGAGAGGAGGGGGACACAGATGTGGTACAGCTCTTGT TACTATGGGAGGGAAATCTGCATTAT

GCCATCATAGGAGCTTTGAAGACTGAGAATTATAATCTAGTATGTGAGTACCATAGC CAAATTCAGGACTGGCATATTCTCCT

ACCCTTGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGGATG TGACCTTATATGCCTTCTCCAACATG

CTGTAAAATGTGATATGCTTTCTATTCTTGTTAAATATAAGGAGGATTTACTAAATG TAAGGATTAGGCATCGTACCCAATCC

CTGTTTGTTTTGGCATGCGAAAATCGGAGATTTGAGATTATTGAATGGATAGGTCAA AATCTGTCAATTCCTGAACCTGAGGC

CATTTTTAGCATTGCTATTGTTACAAAAGATGTAGAACTGTTTTCCTTAGGATATAA AATTATTTTTGATTACATGCAAAGAC

AAGGAATTTTTCAATTAACCAATGTAGTTCGCATGCTTCTGCTAAATCGTCATATTG GTATGGCAATAGAAAAAGGACTTTTA

CCTTTTATTCTGGAAACTTTAAAATATGGTGGTAGTGTAAAAAGAGCTTTATCTTAT GCAGTAATAGATAATAAAAGAAAAAT

TATAGACTATCTTGTACGCCATGAAAATATACCCCGAGGAACTATTGAAAGACTTTT GCATCTAGCTGTGAAAAAACAATCTT

CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTGGTAGAGCATGTAGTAGAT

CACAAATCCACTCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGGCGGTTTTTGATAGACATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAATCACCCATTTTAA AGATCTCTGCAAATATCTTTGGCTGA

ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGCTTGGAAATTGCTGATGAGA AGGAGTTTCCTGATATTAAAAGTATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATC ATGCAAGCTTATGCTTTAGAGTATGA

CATGTATTAA

SEQ ID No. 259 - China/2018/AnhuiXCGQ MGF 5056R

ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACATTTTCTTCCTTCCAAATGATTTT AGCAAGCATACCCTACAATGGCTGGG

ATTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAGACAGCATAATGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACAGCTTGCAGGAGAGGAGGGGGACACAGATGTAGTACAGCTCTTGT TATTATGGGAGGGAAATCTGCATTAT

GCCATCATAGGAGCCTTGAAGACTGAAAAATATAACCTAATATGTGAGTATCATAGC CAAATTCAGGACTGGCATATTCTCCT

ACCCATGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGGATG TGACTTTATTTGCCTTCTCCAACATG

CTGTAAAATACAACATGCTTTCTATTCTTGTCAAATATAAGGAGGATCTACTAAATG CAAGGATTAGGCATCGTATCCAATCC

CTGTTTGTTTTGGCATGCGAAAATCGGAGAATTGAAATTATTGATTGGATAGGCCAA AATCTGCCAATTCCTGAACCTGATGC

CATTTTTAGCATTGCTGTTGCTACAAGAGATTTAGAACTGTTTTCCTTAGGGTACAA GATTATTTTTGATTACATGCAAAGAC

AGGGAATCATTCAATTAACCAATGGAGTTCGCATGGTTGTGCTAAATCGTCACATTA GCATGGCAATAGATAATGGTCTTTTA

CCTTTTGTTCTGGAAACTTTAAAACATGGTGGGAATATACATAGAGCCTTATCTTAT GCAGTAACACACAATAGAAGAAAAAT

TCTGGATTATCTTATTCGCCAGAAAAATATAGCCCCTAATACAATTGAAAGACTTTT ATATCTGGCCGTGAAAAATCAATCTT

CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTGGTAGAGCATGTAGTAAAT

GAGAAATCCACTCTTGTGTTAAAAATTTTATTAGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGGCGATTTTTGGTAGACATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAATCACCCATTTTAA AGACCTCTGCAAATATCTTTGGCTGA

ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGTTTAGAAATTGCTGATGAGA AGGAGTTTCCTGATATTAAAAGTATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATC ATGCAAGCCTATGATGCTTTAGAGTA

G

SEQ ID No. 260 - Georgia 2007/1 MGF 5056R

ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACATTTTCTTCCTTCCAAATGATTTT AGCAAGCATACCCTACAATGGCTGGG

ATTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAGACAGCATAATGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACAGCTTGCAGGAGAGGAGGGGGACACAGATGTAGTACAGCTCTTGT TATTATGGGAGGGAAATCTGCATTAT

GCCATCATAGGAGCCTTGAAGACTGAAAAATATAACCTAATATGTGAGTATCATAGC CAAATTCAGGACTGGCATATTCTCCT

ACCCATGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGGATG TGACTTTATTTGCCTTCTCCAACATG

CTGTAAAATACAACATGCTTTCTATTCTTGTCAAATATAAGGAGGATCTACTAAATG CAAGGATTAGGCATCGTATCCAATCC

CTGTTTGTTTTGGCATGCGAAAATCGGAGAATTGAAATTATTGATTGGATAGGCCAA AATCTGCCAATTCCTGAACCTGATGC

CATTTTTAGCATTGCTGTTGCTACAAGAGATTTAGAACTGTTTTCCTTAGGGTACAA GATTATTTTTGATTACATGCAAAGAC

AGGGAATCATTCAATTAACCAATGGAGTTCGCATGGTTGTGCTAAATCGTCACATTA GCATGGCAATAGATAATGGTCTTTTA

CCTTTTGTTCTGGAAACTTTAAAACATGGTGGGAATATACATAGAGCCTTATCTTAT GCAGTAACACACAATAGAAGAAAAAT

TCTGGATTATCTTATTCGCCAGAAAAATATAGCCCCTAATACAATTGAAAGACTTTT ATATCTGGCCGTGAAAAATCAATCTT

CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTGGTAGAGCATGTAGTAAAT

GAGAAATCCACTCTTGTGTTAAAAATTTTATTAGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGGCGATTTTTGGTAGACATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAATCACCCATTTTAA AGACCTCTGCAAATATCTTTGGCTGA

ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGTTTAGAAATTGCTGATGAGA AGGAGTTTCCTGATATTAAAAGTATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATC ATGCAAGCCTATGATGCTTTAGAGTA

G SEQ ID No. 261 - Mkuzi 1979 MGF 5056R

ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACACTTTCTTCCTTCCAAATGATTTT AGCAATCATACCCTACAACGGCTGGG GTTATATTGGAAAGAGCATGGATCCGTCCATCGAATAGAAAAGGACAGCATAATGATACA GAATGAATTGGTTCTTTCTATCA ATGATGCTTTACATCTTGCAGGAGAGGAGGGGAACACAGATGTAGTACAGCTCTTGTTAT TATGGGAGGGAAATCTGCATTAT GCCATCATAGGAGCCTTGAAGACTGAGAAATATAACCTAATATGTGAGTACCATAGCCAA ATTCAGAACTGGCATGTTCTCCT CCCCTTGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGGATGTGA CTTTATTTGCCTTCTCCAACATG CTGTAAAATGTGACATGCTTTCTATTCTTGTTAAATATAAGGAGGATCTACTAAATGCAA GGATTAGGCATCGTATCCAATCC CTGTTTGTTTTGGCATGCGAAAATCGGAGATTTGAGATTATTGAATGGATAGGTCAAAAT CTGCCAATTCCTGAACCTGAGGC CATTTTTAGCATTGCTATTGTTACAAAAGATATAGAACTGTTTTCCTTAGCGTACAAGCT TATTTTTGATTACATGCAAAGAC AAGGAACTTTTCAATTAACCAATATGGTCCGCATGCTTCTGCTAAATCGGTACATTGGTA TGGCAATAGAAAAAGGACTTTTA C C C T T TAT T CT G G AAAC T T TAAAAT AT GGTGGTAGTG TAAATAGAG C T T TAT C T TAT G CAG TAAT AGAT AATAAAAG AAAAAT TATAGACTATCTTGTACGCCATGAAAATATACCCCGTGGAACTATTGAAAGACTTTTGCA TCTAGCTGTGAAAAAACAATCTT CCAGAAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTAAAA AGCTGGTAGAGCATGTAGTAGAT CACAACTCTACTCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATCTAGTGGATGCT GTTTTAACAAGACTTGTAAAACA TTCTACATATTTCCGGGTAAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAAATT CATTAAAATAGCTGTGCGGGAAA AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAAGAA TTACTTATCTCAAACAGATAGTG CACACCATAAAATATGAAAGTGGAAGGCGGTTTTTGATAGACATCATTCACAGCATTTAC CAAAGTTATTCACTAAAACATGA AGATATTTTTAAACTGGCAACATTTTATGTCAAACACAATGCAATCACCCATTTTAAAGA TCTCTGCAAATATCTTTGGCTGA ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGCTTGGAAATTGCTGATGAGAAGG AGTTTCCTGATATTAAAAGCATT GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATCATG CAAGCCTATAATGCTTTAGAGTA G

SEQ ID No. 262 - Pretorisuskop/96/4 MGF 5056R

ATGTTCTCCCTTCAGGACCTCTGTCGGAAGAACATTTTCTTCCTTCCAAACGATTTT AGCAAGCATACCCTACAATGGCTGGG GTTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAGACAGCATAATGATACA GAATGAATTGGTTCTTTCTATCA ATGATGCTTTACTGCTTGCAGGAGAGGAGGGGGACACAGATGTAGTACAGCTCTTGTTGC TATGGGAAGGAAATCTGCATTAT GCCATCATAGGAGCCTTGAAGACTGAGAAATATAGCCTAGTATGTGAGTACCATAGCCAA ATTCAGGACTGGCATGTTCTCCT CCCCTTGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGAATGTGA CTTTATATGCCTTCTACAACATG CTGTAAAATGTGACATGCTTTCTATTCTTGTTAAATATAAAGAGGATCTACTAAATGTAA GGATTAGGCATCGTATCCAATCC CTGTTTGTTTTGGCATGCGAAAATCGGAGATTTGAGATTATTGAATGGATAGGTCAAAAT CTGCCAATTCCTGAACCTGAGGC CATTTTTAGCATTGCTATTGTTACAAAAGATATAGAACTGTTTTCCTTAGGATACAAGCT TATTTTTGATTACATGCAAAGAC AAGGAACTTTTCAATTAACCAATATGGTTCGCATGCTTCTGCTAAATCGTTACATTGGTA TGGCAATAGAAAAAGGCCTTTTA C C C T T TAT C GT G G AAAC T T TAAAAT AT GGTGGTAGTG TAAATAGAG C T T TAT C T TAT G CAG T CAT AGAT AATAAAAGAAAAAT TATAGACTATCTTGTACGTCATGAAAATATACCCCGTGGAACTATTGAAAGACTTTTGCA TCTAGCTGTGAAAAAACAATCTT CCAGAAAAACTTTGAACTTGTTGCTATCTTACATAAACTACAAGGTGAAAAATGTTAAAA AGCTGGTAGAGCATGTAGTAGAT CACAACTCTACCCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATTTAGTGGATGCT GTTTTAACAAGACTTGTAAAACA TTCTACATATTTCCGGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAAATT CATTAAAATAGCTGTGCGGGAAC AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAAGAA TTACTTATCTCAAACAGATAGTG CACACCATAAAATATGAAAGTGGAAGGCGGTTTTTGATAGACATCATTCACAGCATTTAC CAAAGTTACTCACTAAAACACGA AGATATTCTTAAACTGGCAATATTTTATGTCAAATACAATGCAATCACCCATTTTAAAGA TCTCTGCAAATATCTTTGGCTGA ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGCTTGGAAATTGCTGATGAGAAGG AGTTTCCTGATATTAAAAGCATT GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACTAAGGAAGAAATCATA TAA

SEQ ID No. 263 - Tengani 62 MGF 505 6R

ATGTTCTCCCTTCAGGACCTCTGTCGGAAGAACACTTTCTTCCTTCCAAATGATTTT AGCAAGCATACCCTACAACGGCTGGG

GTTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAGACAGCATAATGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACAGCTTGCAGGAGAGGAGGGGGACACAGATGTAGTACAGCTCTTAT TATTATGGGAAGGAAATCTTCATTAT

GCCATCATAGGAGCCTTGAAGACTGAGAAATATAACCTAATATATGAGTACCATAGC CAAATTCAGGACTGGCATGTTCTCTT

ACCCATGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGCATG TGACTTTATATGCCTTCTCCAACATG

CTATAAAATATAACATGCTTTCTATCCTTGTCAAATATAAGGAAGACTTACTAAATG TAAGGATTAGGTATCGTATCCAATCC

CTGTTTGTTTTGGCATGTGAAAACCGGAGAATTGAGATTATTGAATGGATAGGTCAA AATCTGCCAATTCCTGAACCTGATGC

CATTTTTAGCATTGCTGTTGCTACAAGAGATTTAGAACTGTTTTCCTTAGGGTACAA GATTATTTTTGATTACATGCAAAGAC

AGGGAATCTTTCAATTAACCAATGGAGTTCGCATGGTTGTGCTAAATCGTCACATTA GCATGGCAATAGATAATGGTCTTTTA

CCCTTTGTTCTGGAAACTTTAAAACATGGTGGGAATATACATAGAGCCTTATCTTAT GCAGTAACACACAATAAAAGAAAAAT

TCTGGATTATCTTATTCGCCAGAAAAATATAGCCCCTAATACAATTGAAAGACTTTT ATATCTGGCCGTGAAAAATCAATCAT

CCAGAAAAACTTTGAACTTGTTACTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTGGTAGAGCATGTAGTAAAT

GAGAAATCCACTCTTGTGTTAAAAATTTTATTAGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTGATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGGCGATTTTTGGTAGACATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAACCACCCATTTTAA AGATCTCTGCAAATATCTTTGGCTGA ACAGAGGAACAGAAAGTAAAAAACTCTTTTTAGAGTGTTTAGAAATTGCTGATGAGAAGG AGTTTCCTGATATTAAAAGCATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAGCC TATGGATGCTTTAGAGTAGCCATGTA

TTAA

SEQ ID No. 264 - Warmbaths MGF 5056R

ATGTTCTCCCTACAGGACCTCTGTCGGAAGAACATTTTCTTCCTTCCAAACGATTTT AGCAAGCATACCCTACAATGGCTGGG

GTTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAGACAGCATAATGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACTGCTTGCAGGAGAGGAGGGGGACATAGATGTAGTACAGCTCTTGT TATTATGGGAGGGTAATCTGCATTAT

GCCATCATAGGAGCCTTGAAGACTGAGAAATATAACCTAATATGTGAGTACCATAGC CAAATTCAGGACTGGCATATTCTCCT

ACCCATGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGCATG TGACTTTATATGCCTTCTCCAACATG

CTGTAAAATATAACATGCTTTCTATCCTTGTCAAATATAAGGAAGACTTACTAAATG TAAGGATTAGGTATCGTATCCAATCC

CTGTTTGTTTTGGCATGCGAAAACCGGAGAATTGAGATTATTCATTGGATAGGCCAA AATCTGCCAATTCCTGAACCTGATGC

CATTTTTAGCATTGCTGTTGCTACAAGAGATTTAGAACTGTTTTCCTTAGGGTACAA GATTATTTTTGATTACATGCAAAGAC

AGGGAATCTTTCAATTATCCAATGGAGTTCGCATGGTTGTGCTAAATCGTCACATTA GCATGGCAATAGATAATGGACTTTTA

CCCTTTGTTCTGGAAACTTTAAAACATGGTGGGAATATACATAGAGCCTTATCTTAT GCAGTAACACACAATAGAAGAAAAAT

TCTGGATTATCTTATTCGCCAGAAAAATATAGCCCCTAATACAATTGAAAGACTTTT ATATCTGGCCGTGAAAAATCAATCTT

CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTGGTAGAGCATGTAGTAAAT

GAGAAATCCACTCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGACGATTTTTGATAGAGATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAACACAATGCAACCACCCATTTTAA AGATCTCTGCAAATATCTTTGGCTGA

ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGTTTAGAAATTGCTGATGAGA AGGAGTTTCCTGATATTAAAAGTATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAACTATTACCAAGGAAGAAATC ATGCAAGCCTATGATGCTTTAGAGTA

G

SEQ ID No. 265 - Warthog MGF 5056R

ATGTTCTCCCTTCAGGACCTCTGTCGGAAGAACATTTTCTTCCTTCCAAATGATTTT AGCAAGCATACCCTACAATGGCTGGG

GTTATATTGGAAAGAGCATGGATCCGTCCATCGAGCAGAAAAAAACAGCATAGTGAT ACAGAATGAATTGGTTCTTTCTATCA

ATGATGCTTTACAGCTTGCAGGGGAAGAGGGGGACACAGATGTAGTACAGCTCTTGT TATTATGGGAGGGAAATCTGCATTAT

GCCATCATAGGAGCCTTGAAGACTGAGAAATATAATCTAATATGTGAGTACCATAGC CAAATTCAGGACTGGCATGTTCTCCT

CCCCTTGATTCAAGATCCAGAAACATTCGAAAAATGTCATGATTTAAGCCTTGCATG TGACTTTATATGCCTTCTACAACATG

CTGTAAAATATAACATGCTTTCTATCCTTGTCAAATATAAGGAAGACTTACTAAATG TAAGGATTAGGTATCGTATCCAATCC

CTGTTTGTTTTGGCATGTGAAAACCGGAGAATTGAGATTATTGATTGGATAGGCCAA AATCTGCCAATTCCTGAACCTGATGC

CATTTTTAGCATTGCTGTTGCTACAAGAGATTTAGAACTGTTTTCCTTAGGGTACAA GATTATTTTTGATTACATGCAAAGAC

AGGGAATCTTTCAATTATCCAATGGAGTTCGCATGGTTGTGCTAAATCGTCACATTA GCATGGCAATAGATAATGGACTTTTA

CCCTTTGTTCTGGAAACTTTAAAACATGGTGGGAATATACATAGAGCCTTATCTTAT GCAGTAACACACAATAGAAGAAAAAT

TCTGGATTATCTTATTCGCCAGAAAAATATAGCCCCTAATACAATTGAAAGACTTTT ATATCTGGCCGTGAAAAATCAATCTT

CCAGGAAAACTTTGAACTTGTTGCTATCTTACATAAATTACAAGGTGAAAAATGTTA AAAAGCTAGTAGAGCATGTAGTAAAT

GAGAAATCCACTCTTGTGTTAAAAATTTTATTGGAAAAAAAGGAAAATCTAGTGGAT GCTGTTTTAACAAGACTTGTAAAACA

TTCTACATATTTCCAGGTGAGAGAATTTATCCAGGAGTTTTCCATCAGCCCAGAAAA ATTCATTAAAATAGCTGTGCGGGAAA

AGAAAAATGTGTTAATCGAGGCTATTTCTGAAGATATTTGGGAAAATCCCACAGAAA GAATTACTTATCTCAAACAGATAGTG

CACACCATAAAATATGAAAGTGGAAGACGATTTTTGATAGACATCATTCACAGCATT TACCAAAGTTACTCACTAAAACACGA

AGATATTCTTAAACTGGCAACATTTTATGTCAAATACAATGCAATCACCCATTTTAA AGATCTCTGCAAATATCTTTGGCTGA

ACAGAGGAACAGAAAGTAAGAAACTGTTTTTAGAGTGTTTAGAAATTGCTGATGAGA AGGAGTTTCCTGATATTAAAAGCATT

GTGAGTGAATATATTAACTACTTGTTTACTGCAGGAGCTATTACCAAGGAAGAAATC ATGCAAGCCTATGATGCTTTAGAGTA

G

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Benin 97/1 strain:

SEQ ID No. 55 (MGF 360 6L), SEQ ID No. 68 (MGF 360 10L), SEQ ID No. 78 (MGF 360 11 L), SEQ ID No. 96 (MGF 360 12L), SEQ ID No. 108 (MGF 360 13L) and SEQ ID No. 120 (MGF 360 14L), and

SEQ ID No. 141 (MGF 505 1R) and SEQ ID No. 151 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the China/2018/AnhuiXCGQ strain: SEQ ID No. 52 (MGF 360 6L), SEQ ID No. 63 (MGF 360 10L), SEQ ID No. 76 (MGF 360 11 L), SEQ ID No. 89 (MGF 360 12L), SEQ ID No. 101 (MGF 360 13L), SEQ ID No. 113 (MGF 360 14L) and SEQ ID No. 125 (MGF 360 21 R), and SEQ ID No. 135 (MGF 505 1 R) and SEQ ID No. 148 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Georgia 2007/1 strain:

SEQ ID No. 51 (MGF 360 6L), SEQ ID No. 62 (MGF 360 10L), SEQ ID No. 75 (MGF 360 11 L), SEQ ID No. 88 (MGF 360 12L), SEQ ID No. 100 (MGF 360 13L), SEQ ID No. 112 (MGF 360 14L) and SEQ ID No. 124 (MGF 360 21 R), and SEQ ID No. 134 (MGF 505 1 R) and SEQ ID No. 147 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Ken05/Tk1 strain:

SEQ ID No. 35 (MGF 1106L), SEQ ID No. 44 (MGF 110 8L), and

SEQ ID No. 58 (MGF 360 6L), SEQ ID No. 65 (MGF 360 10L), SEQ ID No. 86 (MGF 360

11 L) and SEQ ID No. 130 (MGF 36021R), and

SEQ ID No. 145 (MGF 505 1 R) and SEQ ID No. 156 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Ken06.Bus strain:

SEQ ID No. 36 (MGF 1106L), and

SEQ ID No. 61 (MGF 360 6L), SEQ ID No. 64 (MGF 360 10L), SEQ ID No. 87 (MGF 360 11 L), SEQ ID No. 99 (MGF 360 12L), SEQ ID No. 111 (MGF 360 13L), SEQ ID No. 123 (MGF 360 14L) and SEQ ID No. 132 (MGF 360 21 R), and SEQ ID No. 144 (MGF 505 1 R) and SEQ ID No. 158 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Kenya 1950 strain:

SEQ ID No. 37 (MGF 1106L), SEQ ID No. 45 (MGF 110 8L), and

SEQ ID No. 60 (MGF 360 6L), SEQ ID No. 66 (MGF 360 10L), SEQ ID No. 85 (MGF 360 11 L), SEQ ID No. 98 (MGF 360 12L), SEQ ID No. 110 (MGF 360 13L), SEQ ID No. 122 (MGF 360 14L) and SEQ ID No. 131 (MGF 360 21 R), and SEQ ID No. 143 (MGF 505 1 R) and SEQ ID No. 157 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the L60 strain: SEQ ID No. 54 (MGF 360 6L), SEQ ID No. 67 (MGF 360 10L), SEQ ID No. 77 (MGF 360 11 L), SEQ ID No. 95 (MGF 360 12L), SEQ ID No. 107 (MGF 360 13L) and SEQ ID No. 119 (MGF 360 14L), and

SEQ ID No. 140 (MGF 505 1 R) and SEQ ID No. 150 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Malawi Lil-20/1 (1983) strain:

SEQ ID No. 38 (MGF 1106L), SEQ ID No. 46 (MGF 110 8L), and

SEQ ID No. 59 (MGF 360 6L), SEQ ID No. 74 (MGF 360 10L), SEQ ID No. 84 (MGF 360 11 L), SEQ ID No. 97 (MGF 360 12L), SEQ ID No. 109 (MGF 360 13L), SEQ ID No. 121 (MGF 360 14L) and SEQ ID No. 133 (MGF 360 21 R), and SEQ ID No. 146 (MGF 505 1 R) and SEQ ID No. 155 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Mkuzi 1979 strain:

SEQ ID No. 39 (MGF 1106L), SEQ ID No. 47 (MGF 110 8L), and

SEQ ID No. 57 (MGF 360 6L), SEQ ID No. 69 (MGF 360 10L), SEQ ID No. 79 (MGF 360 11 L), SEQ ID No. 94 (MGF 360 12L), SEQ ID No. 106 (MGF 360 13L), SEQ ID No. 118 (MGF 360 14L) and SEQ ID No. 128 (MGF 360 21 R), and SEQ ID No. 138 (MGF 505 1 R) and SEQ ID No. 149 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Pretorisuskop/96/4 strain:

SEQ ID No. 40 (MGF 1106L), SEQ ID No. 48 (MGF 110 8L), and

SEQ ID No. 56 (MGF 360 6L), SEQ ID No. 70 (MGF 360 10L), SEQ ID No. 81 (MGF 360 11 L), SEQ ID No. 93 (MGF 360 12L), SEQ ID No. 105 (MGF 360 13L), SEQ ID No. 117 (MGF 360 14L) and SEQ ID No. 127 (MGF 360 21 R), and SEQ ID No. 142 (MGF 505 1 R) and SEQ ID No. 153 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Tengani 62 strain:

SEQ ID No. 41 (MGF 1106L), and

SEQ ID No. 71 (MGF 360 10L), SEQ ID No. 82 (MGF 360 11 L), SEQ ID No. 90 (MGF 360 12L), SEQ ID No. 102 (MGF 360 13L), SEQ ID No. 114 (MGF 360 14L) and SEQ ID No. 126 (MGF 36021 R), and

SEQ ID No. 136 (MGF 505 1 R) and SEQ ID No. 152 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Warmbaths strain: SEQ ID No. 42 (MGF 1106L), SEQ ID No. 49 (MGF 110 8L), and

SEQ ID No. 53 (MGF 360 6L), SEQ ID No. 73 (MGF 360 10L), SEQ ID No. 80 (MGF 360 11 L), SEQ ID No. 92 (MGF 360 12L), SEQ ID No. 104 (MGF 360 13L), SEQ ID No. 116 (MGF 360 14L) and SEQ ID No. 129 (MGF 360 21 R), and SEQ ID No. 137 (MGF 505 1 R) and SEQ ID No. 154 (MGF 5052R).

In an embodiment the invention provides an ASFV which comprises one or more, such as all, of the following sequences from the Warthog strain:

SEQ ID No. 43 (MGF 1106L), SEQ ID No. 50 (MGF 110 8L), and

SEQ ID No. 72 (MGF 360 10L), SEQ ID No. 83 (MGF 360 11 L), SEQ ID No. 91 (MGF 360 12L), SEQ ID No. 103 (MGF 360 13L), SEQ ID No. 115 (MGF 360 14L), and

SEQ ID No. 139 (MGF 505 1 R).

The translation products (i.e. protein sequences) of these genes are given below:

Benin 97/1 MGF gene protein sequences

SEQ ID No. 1 - translation of Benin 97/1 MGF 110-11L (A9JKR9) MKYSWKNGGGDYWPIIIRHCCFYLVFSIAFVGYIVFAYYKNLHLNTAMKLLALLCILIWL SQPGLNRPLSIFYMKQNLPRTYT

PPIRELEYWCTYGKHCDFCWECRNGICKNKVWDDMSSVQEHSYPMEHCMIHRQCKYI RDGPIFQVECTMQTSDATHLINA

SEQ ID No. 2 - translation of Benin 97/1 MGF 110-12L (A9JKS1)

MKVFLGLLLGYSTILILTYQSPTTQWCFYEISLKILNHHSMEKWRDKNWSIIIRYYC FYLVFSFAFAGCVAFAICKNLRLCTT

MKLLMLLNILVLLSQPILNN SEQ ID No. 3 - translation of Benin 97/1 MGF 360-6L (A9JKS8)

MNSLQVLTKKVLIENKAFSEYHEDDIFILQQLGLWWHNGPIGFCKQCKMVTSGSMSC SDVDSYELDRALVRAVKKNQTDLIKL FVLWGANINYGIICAKTERTKDLCIELGANPEFLDVGLYNMFVDLIKQQKVLLAIDIYYD NISILDSFDSHDFYVLIDFIYNC FILNLDEKEKMIKNTYVLKFWFKIAIEFNLIKPIRFLSKKFPHLDYWRLKTAVYLGNVDE IHHAYFQENIRLDPNDMMSLACM YPQNKLGIYYCFALGANINTALETLIGFINHEVNREITFFSNYGIWSNVHFCISLGANPY TKKIQETLLRQEKNVIMKLLFKK GLLSPHSILHKKILEPSEVRKIISTYEYTETFHSFSLLRDNLR

SEQ ID No. 4 - translation of Benin 97/1 MGF 360-10L

MVPSLQSFAKKVLASQHVSIDYHVILERCGLWWYKAPISLDCKHMLIKLPNFADGLD LNTALMLATKENNYQLIKMFTDWGAD INYGLICANTPPIREFCWELGAKYQVDKKKIMHIFFKLIHPNTTSNNIILCLKFFNDNPF SAYVIIREIKSCIHWKLKNLAED TNVLSNISDGDMLTIYCFIVALQDNLREAISYVYQHFKYLNTWWLTCALCYNKLFDLHNL YEKEKIRMDMDEMMRIACTKDNN FLTIYYCFILGANINLAMIASIRFYNMDNLFFCIDLGADAFEEAKALAEQQNYYLISHRL SLDIYSPDSSLLTLKEADPNKIY RLLKNYKSKSMLAYLNYDINDTSL

SEQ ID No. 5 - translation of Benin 97/1 MGF 360-11L (A9JKU4)

MLPSLQSLTKKVLAGQCVSVDHYHILKCCGLWWHNGPIMLHIRRNKLFIRSTCFSQG IELNIGLMKAVKENNHDLIKLFTEWG ADINYGMICALTENTRDLCKELGAKEYLEREYILKIFFDTTRDKTSSNIIFCHEVFSNNP NLRIIDNLDLRGEIMWELRGLME ITFMLDHDDSFSTVLTKYWYAIAVDYDLKDAIRYFYQKYPRLHRWRLMCALFYNNVFDLH ELYEIERVRMDIDEMMHIACIQD YSYSAIYYCFIMGANINQAMLVSIQNYNLGNLFFCIDLGANAFEEGKALAEQKENYLIAH ALSLKHYNPVISLLSNVMDPEKI NYMLKNYHSINMGIFLDYEQR

SEQ ID No. 6 - translation of Benin 97/1 MGF 360-12L (A9JKU9) MLPSLQSLTKKVLAGQCVPTNQHYLLKYYDLWWYNAPITFDHNLRLIKSSGIKEGLDLNT ALVKAVRENNYSLIKLFTEWGAD INYGLVSWTEHTRDLCQELGAKEILNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPILQ KVNNLKLRIEIFWELRELIEKT DLLNNEFLLSTLLLKYWYAIAVRYSLKEAIQYFYQKYTHMNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYMLGANINQAMLTSIQYYNIENMFFCMDLGADVFEEGTTALGEGYELIKNILS LKIYSPTTIPLPKSTDPEIIDHA LKNYFSKNMMIFLSYDLR

SEQ ID No. 7 - translation of Benin 97/1 MGF 360-13L (A9JKU7)

MSLPLSLQTLVKKTVASQCLSIDEHCILKYCGLWWHDAPLKLCMDRGRIQIKSGFLG EDIDLRVALIIAVKENNYSLIKLFTE WGANINYSLLSINTKHIRELCRQLGAKETLEDNDIFRIFTRIMHNKTSGSIILCHEIFMN NPMLENKFVIQLRGLIYKRLWGL IEIKETDELNDLLVKYWYAKAVQYVCKNAICFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKKKVQIDVHDMICLACAKDNN LLTIYYCYALGGNINQAMLTSVQYYNVGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL TLDIYSSDASLPLNLKDPEKISS LLKDYKSKNLSIIWEYSHNIL

SEQ ID No. 8 - translation of Benin 97/1 MGF 360-14L (A9JKV1)

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVANLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYKRHKNQLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNMKRAEEYLTAHPEIIVID

SEQ ID No. 9 - translation of Benin 97/1 MGF 505-1 R (A9JKU5)

MFSLQNLCRKTLPDCKLPEFFDDYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRIAASEENYEIVGLLLAWEGNL YYAIIGALEGNRYNLIRKYDDQIKDHHDILPFIDDPIIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYTLEDDLPLVHLL IEKACEDHNYEVIKWIYENLHVCHIIDTFDCAIAHKDLRLYCLGYTFIYNRIVPYKYHHL DILILSSLQLLHKVAAKGYLDFI LETLKYDHNIDNLDVILTQAATYNHRKILTYFIPQSTYAQIEQCLFVAIKTKSSKKTLNL LLSHLNLSIKLIQKISQYVATFN STNIIGILSMKRKKKIYLDIILTKFVKNAIFNKFW RCMERFSINPERIVKMAARINKMMLVKKISEHVWKNHAARLKHLKHA VHTMKHKDGKNRLMNFIYEHCYYHMQGEEIFSLARFYAIHHAPKLFDVFYNCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFAMGVLSKKEILQDYPSIYSKHYMP

SEQ ID No. 10 - translation of Benin 97/1 MGF 505-2R (A9JKV3)

MFSLQDLCRKHLFILPDVFGEHVLQRLGLYWRCHGSLQRIGDDHILIRRDLILSTNE ALRMAGEEGNNEW KLLLLWKGNLHY AVIGALQGDQYDLIHKYENQIGDFHFILPLIQDANTFEKCHALERFCGVSCLLKHATKYN MLPILQKYQEELSMRAYLHETLF ELACLWQRYDVLKWIEQTMHVYDLKIMFNIAISKRDLTMYSLGYIFLFDRGNTEATLLTQ HLEKTAAKGLLHFVLETLKYGGN IDTVLTQAVKYNHRKLLDYFLRQLPRKHIEKLLLLAVQEKASKKTLNLLLSHLNYSVKRI KKLLRYVIEYESTLVIKILLKKR VNLIDAMLEKMVRYFSATKVRTIMDELSISPERVIKMAIQKMRTDIVIHTSYVWEDDLER LTRLKNMVYTIKYEHGKKMLIKV MHGIYKNLLYGEREKVMFHLAKLYVAQNAATQFRDICKDCYKLDVARFKPRFKQLILDCL EIVTKKSCYSILEILEKHIISLF TMKVMTEEEKNLCLEILYKVIHYKTIQC

MGF 1105L protein sequences

SEQ ID No. 306 - Ken05/Tk1 MGF 110 5L protein

MLVIFLGLLGLLANQVLGLPNQPAGQLHPTDNPPKEELGYWCTYTESCKFCWNCQNG LCEGKLENTTILENEYVQSCIVSRWL

NKCMYDLGQGIDHVMACSEPKPWNPYKILKREWKKNVSQN

SEQ ID No. 307 - Ken06.Bus MGF 110 5L protein

MLVIFLGLLGLLANQVLGLPNQPAGQLHPTDNPPKEELGYWCTYTESCKFCWNCQNG LCEGKLENTTILENEYVQSCIVSRWL

NKCMYDLGQGIHHVMPCSEPKPWNPYKILKKEWKENVSQN

SEQ ID No. 308 - Malawi Lil-20/1/1983 MGF 110 5L protein

MLVIFLGILGLLANQVSSQLVGQLHPTENPSENELEYWCTYMECCQFCWDCQDGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNSQN

SEQ ID No. 309 - Mkuzi 1979 MGF 110 5L protein

MLVIFLGILGLLANQVSSQLVGQLHPTENPSENELEYWCTYMECCQFCWDCQDGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNSQN SEQ ID No. 310 - Pretorisuskop/96/4 MGF 110 5L protein

MLVIFLGILGLLANQVSSQLVGQLHPTENPSENELEYWCTYMECCQFCWDCQNGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNSQNK

SEQ ID No. 311 - Tengani62 MGF 110 5L protein

MLVIFLGILGLLVNQVSSQPVGQLHPTENPSENELEYWCTYMECCQFCWDCQNGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNS

SEQ ID No. 312 - Warmbaths MGF 110 5L protein

MLVIFLGILGLLANQVSSQLVGQLHPTENPSENELEYWCTYMECCQFCWDCQNGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNSQNK

SEQ ID No. 313 - Warthog MGF 110 5L protein

MLVIFLGILGLLANQVSSQLVGQLHPTENPSENELEYWCTYMECCQFCWDCQNGLCV NKLGNTTILENEYVHPCIVSRWLNKC

MYDLGQGIDHVMVCSQPKYWNPYKILKKEWKENNSQNK

SEQ ID No. 314 - China/2018/AnhuiXCGQ MGF 110 5L/6L fusion protein

MLVIFLGILGLLASQVSSQLVGQLRPTEEPPEEELEYWCAYMESCQFCWDCQDGTCI NKIDGSVIYKNEYVKSCLVSRWLDKC MYDLDKGIYHTMNCNQVLGLPNQPAGQLHPTDNPPQEELEYWCTYTENCKFCWNCQNGLC EGKLENTTILENEYVQSCIVSRW LNKCMYDLGQGIHHVMACSEPKPWNPYKILKREWKENNS

SEQ ID No. 315 - Georgia 2007/1 MGF110 5L/6L fusion protein

MLVIFLGILGLLASQVSSQLVGQLRPTEEPPEEELEYWCAYMESCQFCWDCQDGTCI NKIDGSVIYKNEYVKSCLVSRWLDKC MYDLDKGIYHTMNCNQVLGLPNQPAGQLHPTDNPPQEELEYWCTYTENCKFCWNCQNGLC EGKLENTTILENEYVQSCIVSRW LNKCMYDLGQGIHHVMACSEPKPWNPYKILKREWKENNS

MGF 11012L protein sequences

SEQ ID No. 316 - Benin 97/1 MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPTTQWCFYEISLKILNHHSMEKWRDKNWSIIIRYYC FYLVFSFAFAGCVAFAICKNLRLCTT

MKLLMLLNILVLLSQPILNN

SEQ ID No. 317 - China/2018/AnhuiXCGQ MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPTTPWCFYEISLKIPNHHSMKCCSYPRLYEHEMFME KWRDKNWPIIIRYYCFYLVFSFVFAG CVAFAICKNLRLSTTMKLLMLLSILVLLLSQPILNN

SEQ ID No. 318 - Georgia 2007/1 MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPTTPWCFYEISLKIPNHHSMKCCSYPRLYEHEMFME KWRDKNWPIIIRYYCFYLVFSFVFAG CVAFAICKNLRLSTTMKLLMLLSILVLLLSQPILNN

SEQ ID No. 319 - Ken05/Tk1 MGF 110 12L protein

MKVFLGLLLGFSIILILTYQSPTTQHPPKEELAYWCTYAKSCDFCWDCQNDTCINKV INESISITSIVNCRVTRDSQSCFYEI SVKIPNHHSMECSYPRLYEHEMFMEKWRDEYWPIIIKQCCFYLVFSIAFAGCVAFAICKN LRLRTTIKLLILLSILVWLSQPV LN

SEQ ID No. 320 - Ken06.Bus MGF 110 12L protein MKVFLALLLGYLTILILTYQTPTTQHPPKEELPYWCTYVKNCDLCWDCQDSIYWNKVISE SISINSIINCRVTCDSQSQSCFY

EILLKIPNHHSMECSYPGSYENEMFMEKWRDENWSIIIKHYCFYLVFSFAFAGCVAF AICKNLRLSTTMKLLMLLSILVCLSQ

PILNN

SEQ ID No. 321 - Kenya 1950 MGF 110 12L protein MKVFLGLLLGFSIILILTYQSPTTQHPPKEELAYWCTYAKSCDFCWDCQNDTCINKVINE SISITSIVNCRVTRDSQSCFYDI SVKIPNHHSMECSYPRLYEHEMFMEKWRDEYWPIIIKQCCFYLVFSFAFAGCVAFAICKN LRLRTTIKLLILLSILVWLSQPI LNN

SEQ ID No. 322 - L60 MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPTTQWCFYEISLKILNHHSMEKWRDKNWSIIIRYYC FYLVFSFAFAGCVAFAICKNLRLCTT MKLLMLLNILVLLSQPILNN

SEQ ID No. 323 - Malawi Lil-20/1/1983 MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPTTQHPPKEELEYWCTYAKTCDFCWDCQNDTCINKV INESISMNSIVNCRVTRDSQSQSCFY

EISLKIPNYHSMECSYPRLYKHFMSMEKWRDENWPILIRHYCFYLVFSFAFAGCVAF AICKNLRLRTTMKLLMLLSILVLLSQ

PILNN SEQ ID No. 324 - Mkuzi 1979 MGF 110 12L protein

MNATMKVFLGLLLGYSTILILTYQSPTTQWCFYEISLKILNHHSMEKWRDKNWSIII RYYCFYLVFSFAFAGCVAFAICKNLR

LCTTMKLLMLLGILVLLSQPILNN

SEQ ID No. 325 - Warmbaths MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPATQWCFYEISLKIPNHHSMECSYPRLYKHFIFMEK WRDKNWSIIIRYYCFYLVFSFAFAGC IAFAICKNLRLCTTMKLLMLLSILVLLSQPILNN

SEQ ID No. 326 - Warthog MGF 110 12L protein

MKVFLGLLLGYSTILILTYQSPATQWCFYEISLKIPNHHSMECSYPRLYKHFMFMKK WRDKNWSIIIRYYCFYLVFSFAFAGC IAFAICKNLRLCTTMKLLMLLSILVLLSQPILNN

MGF 36012L protein sequences SEQ ID No. 159 - Georgia 2007/1 MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDAPITFDHNLRLIKSAGIKEGLN LNTALVKAVRENNYNLIKLFAEWGAD INYGLVSVNTEHTWDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIVKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYVLGANINQAMLSSIQYYNIENMFFCIDLGADVFEEGTTALGEGYELIKNILS LKIYSPATTPLPKSTDPEIIDHA LKNYVSKNMMIFLTYDLR*

SEQ ID No. 160 - China/2018/AnhuiXCGQ MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDAPITFDHNLRLIKSAGIKEGLN LNTALVKAVRENNYNLIKLFAEWGAD INYGLVSVNTEHTWDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIVKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYVLGANINQAMLSSIQYYNIENMFFCIDLGADVFEEGTTALGEGYELIKNILS LKIYSPATTPLPKSTDPEIIDHA LKNYVSKNMMIFLTYDLR*

SEQ ID No. 161 - Tengani 62 MGF 360 12L protein MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDAPITFDHNLRLIKSAGIKEGLNLNT ALVKAVRENNYNLIKLFAEWGAD INYGLVSVNTEHTWDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIEKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYVLGANINQAMLSSIQYYNIENMFFCIDLGADVFEEGTTALGEGYELIKNILS LKIYSPATTPLPKSMDPEIIDHA LKNYVSKNMMIFLTYDLR*

SEQ ID No. 162 - Warthog MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDGPITFDHNLKLIKSAGIKEGLD LNTALVKAVRENNYNLIKLFAEWGAN INYGLVSVNTEHTRDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIEKT DLLNNEFSLSALLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYVLGANINQAMLTSIQYYNIENMFFCMDLGADAFEEGTIALGEGYKLIKNILS LKIYSPATTPLPKSTDPEIIDHA LKNYVSKNMMIFLTYDLR*

SEQ ID No. 163 - Warmbaths MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDGPITFDHNLKLIKSAGIKEGLD LNTALVKAVRENNYNLIKLFAEWGAD INYGLVSVNTEHTRDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIEKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIYMDIEEMMRIACIKDHN LSTMYYCYVLGANINQAMLTSIQYYNVENMFFCMDLGADAFEEGTIALGEGYKLIKNILS LKIYSPATTPLPKSTDPEIIDHA VKNYVSKNMMIFLTYDLR*

SEQ ID No. 164 - Pretorisuskop/96/4 MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKCYDLWWHDGPITFDHNLKLIKSAGIKEGLD LNTALVKAVRENNYNLIKLFAEWGAN INYGLVSVNTEHTWDLCRELGAKETLNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIEKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYVLGGNINQAMLTSIQYYNIENMFFCMDLGADAFEEGTIALGEGYKLIKNILS LKIYSPATTPLPKSTDPEIIDHA LKNYVSKNMMIFLTYDLR*

SEQ ID No. 165 - Mkuzi 1979 MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKYYDLWWHDAPITFDHNLRLIKSAGIKEGLD LNTALVKAVKENNYNLIKLFAEWGAD INYGLVSVSSEHTWDLCRELGAKETLNEKEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNIKMRIEIFWELRELIEKT DLLNNEFSLSTLLLKYWYAIAIRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIYMDIEEMMRIACIKDHN LSTMYYCYMLGANINQAMLTSIQYYNIENMFFCMDLGADVFEEGTTALGEGYELIKNILS LKIYSPTTIPLPKSTDPEIIDHA LKNYFSKNMMIFLSYDLR*

SEQ ID No. 166 - L60 MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKYYDLWWYNAPITFDHNLRLIKSSGIKEGLD LNTALVKAVRENNYSLIKLFTEWGAD INYGLVSVNTEHTRDLCQELGAKEILNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNLKLRIEIFWELRELIEKT DLLNNEFLLSTLLLKYWYAIAVRYSLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYMLGANINQAMLTSIQYYNIENMFFCMDLGADVFEEGTTALGEGYELIKNILS LKIYSPTTIPLPKSTDPEIIDHA LKNYFSKNMMIFLSYDLR*

SEQ ID No. 6 - Benin 97/1 MGF 360 12L protein

MLPSLQSLTKKVLAGQCVPTNQHYLLKYYDLWWYNAPITFDHNLRLIKSSGIKEGLD LNTALVKAVRENNYSLIKLFTEWGAD INYGLVSVNTEHTRDLCQELGAKEILNEEEILQIFIDLKFHKTSSNIILCHEVFSNNPIL QKVNNLKLRIEIFWELRELIEKT DLLNNEFLLSTLLLKYWYAIAVRYSLKEAIQYFYQKYTHMNTWRLTCALCFNNVFDLHEA YEKDKIHMDIEEMMRIACIKDHN LSTMYYCYMLGANINQAMLTSIQYYNIENMFFCMDLGADVFEEGTTALGEGYELIKNILS LKIYSPTTIPLPKSTDPEIIDHA LKNYFSKNMMIFLSYDLR*

SEQ ID No. 167 - Malawi Lil-20/1 MGF 360 12L protein

MLPSLQSLTKKVLAGQCLPTDQYYLLKCYDLWWYDSPITFDHNLGLIKSAGIKDGLD LNTALVKAVRENNYNLIKLFTEWGAD INYGLVSVNTEHTRDLCRELGAKETLNEEEILRIFIDLKFYKTSSNIILCHEVFSNNPLL QKVNNLKMRIEIFWELRELIKKT DLLNNEFSLNTLLLKYWYAIAVRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKIYMDLEEMMRVACIKDHN LSTIYYCYVLGANINQAMLASIQYYNIENMFFCMDLGADVFEENMPVGEGYELIRNILSL KIYSPSTAPLPKNTDPEIIDHVL KNYKSKNMMTFLSYDLR*

SEQ ID No. 168 - Kenya 1950 MGF 360 12L protein MLPSLQSLTKKVLAGQCLPEDQHYLLKCYDLWWNNAPITFDHNLRLIKSAGLQEGLDLNM ALVKAVKENNYSLIKLFTEWGAN INYGLISVNTEHTWDLCRELGAKKTLNEGDILQIFIDLKFHKTSSNIILCHEVFSDNLLL KKVNNLKMRIEIFWELREIIEKT DLLNNEFSLNTLLLKYWYAIAVRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKICMDLEEMMRIACIKDHS LSTIYYCYMLGANINQAMLTSIQYYNIENIFFCMDLGADAFEEGMALVGQEGYEPIRNIL SLKIYSPATTPLPKSTDPEIIDH ELKNYFSKNMMVFLTYDLR*

SEQ ID No. 169 - Ken06.Bus MGF 360 12L protein

MLPSLQSLTKKVLARQCLPEDQHYLLKCYDLWWNNAPITFDHNLRLIKLAGIQEGLD LNMALVKAVKENNYSLIKLFTEWGAN INYGLISVNTEHTWDLCRELGAKKTLNEGDILQIFIDLKFYKTSSNIILCHEVFSDNLLL KRVNNLKMRIEIFWELREIIEKT DLLNNEFSLNTLLLKYWYAIAVRYNLKEAIQYFYQKYTHLNTWRLTCALCFNNVFDLHEA YEKDKICMDLEEMMRIACIKDHN LSTIYYCYMLGANINQAMLTSIQYYNIENIFFCMDLGADAFEEGMALVGQEGYEPIRNIL SLKIYSPATTPLPKSTDPEIIDH ALKNYFSKNMMVFLTYDLR*

MGF 36013L protein sequences

SEQ ID No. 170 - Georgia 2007/1 MGF 360 13L protein

KSLPLSLQTLVKKTIASQCLSIDEHCILKYCGLWWHDAPLKLCKDRGRIQIKSGFLG EDIDLRVALIIAVKENNYSLIKLFTE WGANINYGLLSINTKHIRELCRQLGA-KETLEDNDIERIFTRIMHNKTSGSIILCHEIFM NNPILSNKEVIQLRGLIYKRLWGL lEIKETDELNGLLVKYWYAKAVQYDCKDAICFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKENIOIDVHDMICLASTKDNN PLTIYYCYALGGNINQAMLT3VQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL ALDIYSSDASLPLNLKDPEEISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 171 - China/2018/AnhuiXCGQ MGF 360 13L protein

MSLPLSLQTLVKKTIASQCL3IDEI-ICILKYCGLWWHDAPLKLCMDRGRIQIK3GF LGEDIDLRVALIIAVKENNYSLIKLFTE WGANINYGLLSINTKHIRELCRQLGA.KFTLEDNDIERIFTRIMHNKTSGSIILCHEIFM NNPILFNKEVIQLRGLIYKRLWGL IETKETDELNGLLVKYWYAKAVQYDCKDAJCFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKENIQIDVHDMICLASTKDNN

PLTIYYCYALGGNINQAMI.T3VQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYNFL SHSLALDIYSSDASLPLNLKDPEEISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 172 - Tengani 62 MGF 360 13L protein

MSLPLSLQTLVKKTVANQSL3IDEI-ICILKHCGLWWHDVPLKLCMDRGQIQIK3GF LGEDIDLHIALIIAVKENNYSLIKLFTE WGANINYSLLSINTKHIRELCRQLGAKETLEDDDIFRIETKIMHNKTSGSIILCHDIEMN NPNIEDKFTIQLRGLIYKRLWGL IEJKETDELNGLLVKYN'YAKAVQYECKDAJCFLDEKYTDLNEWRLKCLLYYNKIYELHE iMYKKENiQIDVHDMICLASTKDNN PLTIYYCYALGGNINQAMLTSVQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSH3L ALDIYSSDASLPLNLKDPEEISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 173 - Warthog MGF 360 13L protein

KSLPL3LQTLVKKTVASQCL3TDEHCILKHCGLWWHDVPLKLCKDRGQIQIK3GFLG EDIDLHIALIIAVKENNYSLIKLFTE WGAHINYSLLSINTEHIRELCRQLGAKETLEDNDIFRIFTKIMHNKTSGRIILCHEIEMN NPNIENKFTIQLRGLICKRLWGL IElKETDELNDLLVRYWYAKAVQYECKDAICFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKEKVQIDVHDMICIASTKDNN PLTIYYCYALGGNINQAMLTSVQYYNIGNIFFCIDLGGNAFEEGPAIAEQKGYHFLSH3L TLDIYSSDASLPLNLKDPEEISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 174 - Warmbaths MGF 360 13L protein

MSLPLSLQTLVKKTVASQCLSTDEHCILKHCGLWWHDVPLKLCMDRGQIQIKSGFLG EDIDLHVALIIAVKENNYSLIKLFTE

WGAHINYSLLSINTEKIRELCRQLGAKKTLEDNDIFRIFTKIMHNKTSGRIILCHEI FMNNPNIENKFTIQLRGLICKRLWRL IElKETDELNDLLVKYWYAKAVQYECKDAICFLDEKYTGLNEWRLKCLLYYNKIYELHEM YHKDKVQIDVHDMICLASTKDNN PLTIYYCYALGGNINQAI^LTSVQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYHFLSH3 LALDIYSSDASLPLNLKDPEEISS LLKGYKSKNLSIIWEYSHNIL*

SEQ ID No. 175 - Pretorisuskop/96/4 MGF 360 13L protein

MSLFLSLQTLVKKTVASQCLSIDEHCILKHCGLWWHDVPLKLCMDRGQIQIKSGFLG EDIDLHIALIIAVKENNYSLIKLETE

WGAHINYSLLSINTEHIRELCRQLGAKETLEDDDVFEIFTKIMHNKTSGRIILCHDI EMNNPNIENKFTIQLRGLICKRLWGL

IEIKETDELNDLLVKYWYAKAVQYECKDAICFLEEKYTDLNEWRLKCLLYFNKIYEL HEMYHKEKVQIDVHDMICLASTKDNN PLTIYYCYALGGNINQAMLTSIQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL ALDIFSSDASLPLNLKDPEEISS

FLKDYKSKMLSIIWEYSHNIL*

SEQ ID No. 176 - Mkuzi 1979 MGF 360 13L protein

MSLPLSLQTLVKKTVASQCLSIDEHCILKYCGLWWHDAPLKLCKDRGRIQIKSGFLG EDIDLRVALIIAVKENNYSLIKLFTE WGANINYSLLSINTKHIRELCRQLGAKETLEDNDIERIFTRIMHNKTSGSIILCHEIFMN NPILFNKEVIQLRGLIYKRLWGL IEIKETDKLNDLLVKYWYAKAVQYVCKNAICFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKKKVQIDVHDMICLACAKDNN LLTIYYCYALGSNINQAMLT3VQYYNIGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL TLDIYSSDASLPLNLKDPEEISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 177 - L60 MGF 360 13L protein

MSLPLSLQTLVKKYVASQCLSIDEHCILKYCGLiJViHDAPLKLCMDRGRIQIKSGF LGEDIDLRVALIIAVKENNYSLIKLFTE WGAMINYSLLSINTKHIRELCRQLGAKFTLEDNDIERIFTRIMHNKTSGSIILCHEIFMN NPMLFNKEVIQLRGLIYKRLWGL lEIKETDELNDLLVKYWYAKAVQYVCKNAICFLDEKYTDLNEWRLKCLLYYNKIYELHEM YHKKKVQIDVHDMICLACAKDNN LLTIYYCYALGGNINQAMLTSVQYYNVGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL TLDIYSSDA3LPLNLKDPEKISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 7 - Benin 97/1 MGF 360 13L protein

MSLPLSLQTLVKKTVASQCL3IDEHCILKYCGLWWHDAPLKLCMDRGRIQIK3GFLG EDIDLRVALIIAVKENNYSLIKLFTE, WGANINYSLLSINTKHIRELCRQLGAKETLEDNDIERIFTRIMHNKTSGSIILCHEIFMN NPMLENKFVIQLRGLIYKRLWGL IFJIKEYDELNDLLVKYWYAKAVQYVCKNAICFLDEKYYDLNEWRLKCLLYYNKIYELHE !MYHKKKVQIDVHDMICLAOAKDNN LLTIYYCYALGGNINQAMLT3VQYYNVGNIFFCIDLGGNAFEEGRAIAEQKGYNFLSHSL TLDIYSSDASLPLNLKDPEKISS LLKDYKSKNLSIIWEYSHNIL*

SEQ ID No. 178 - Malawi Lil-20/1 (1983) MGF 360 13L protein

MSAPLSLQTLVKKTVASTSCLSIDEHILKYCDLWWHDAPLKLYMDRGRIQIK3GFLG EDIDLCVALIIAVKENNYNLIKLFTE LGANINYSLLSINTKHVRDLCRQLGAKETLEDYDIFCIFNKIMHNKTSGSVILCHEIFIN NPNLENKFAAQLRRLIYKRLCGL lEIKETDELSELLVKYWYAKAVQYDYKDAICFLDSKYTDLNEWRLKCYLYYNKIYELHDI YHKEKIQIDVNEMLSLACIRDNN PLTIYYCYALGGNINQAI^LTSVQYYNIGINIYFCIDLGGNAFEEGSAIARQKGYNFLSH SLVLNIYSSDA3LPLNLKDPEEISS LLKNYKSKNLSIILDYSHNIL*

SEQ ID No. 179 - Kenya 1950 MGF 360 13L protein

MSSPL3LQTLVKKTVASTSCL3IDEHILKYCGLWWHDAPLKLYIDRGRIYIKSGFLG EDIDLCVALIIAVKENNYSLIKLFTE

WGAYINYSLLSINTKKARDLCRQLGAKETLDDYDIFCIFNKIMHNKTSGSIILCHEI FINNPKLENNFAAQLRRLIYKRLCGL lEIKETDELSELLVKYWYANAVQYDHKDAICFLDEKYTDLDEKRLKCYLCYNKIYELHDI YHKKKIQIDVNEMLSLACIRDNN LLTIYY ^' CYALGGNINQAJSLTSVQYYNIGNIYFCIDLGGN.AFEHGSAIARQNGYNFLCH SLILNIYSSDASLPLNLiCVPEEISS LLKNYKSKNLS11LDYSHKIL*

SEQ ID No. 180 - Ken06.Bus MGF 360 13L protein

MSSPLSLQTLVKKTVAGTSCLSIDEHILKYCGLWKHDAPLKLYIDRGRIYIKSGFLG EDIDLCVALIlAVKENNY ^' SMIKLETE W!GAYINYSLLSINTKHARDLCRQLGAKETLDDYDIFCIFNKIMHNKTSGSIILCHEIFI NNPNLENNEAAQLRRLIYKRLCGL IEIKETDELSELLVKYWYANAVQYDHKDAICFLDEKYTDLDEW*LKCYLCYNKIYELHDI YHKEKIQIDVNEML$LACIRDNN LLTIYYCYALGGNINQAMLTSVQYYNIGNIYFCIDLGGNAFEEGSAIARQNGYNFLSHSL VLNIYSSDASLPLNLKDPEEISS LLKNYKSKNLS11LDYSHKIL*

MGF 36014L protein sequences

SEQ ID No. 181 - Georgia 2007/1 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVARLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHHLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNLKRAEEYLTAHPEIIVID* SEQ ID No. 182 - China/2018/AnhuiXCGQ MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVARLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHHLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNLKRAEEYLTAHPEIIVID*

SEQ ID No. 183 - Tengani 62 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQIFSYKHVQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVHLKRLCTELGGLTPVSESRLLEILKEVARLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHHLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLQKKNYAA IYYCYRLGASLDYGMNLSIYNNNTLNMFFCIDLGATDFDRAQRIAHKAYMYNLSNIFLVK QLFSRDVTLALDVTEPQEIYDML KSYTSKNLKRAEEYLTAHPEIIVID*

SEQ ID No. 184 - Warthog MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYMLQRFGLWWDLGPIHLCNNCKQIFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVHLKRLCTELGGLTPVSEPRLLEILKEVAKLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHHLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLRKKNYAA IYYCYRLGASLDYGMNLSIYNNNTLNMFFCIDLGATDFDRAQRIAHKTYMYNLSNIFLVK QLFSRDVTLALDVTEPQEIYDML KSYTSKNLKRAEEYLTAHPEIIVID*

SEQ ID No. 185 - Warmbaths MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYMLQRFGLWWDLGPIHLCNNCKQIFSYKHLQCFSED DLCLEAALVKAVKRDNLELIRLFVD WGANPEYGLICVPTVHLKRLCTELGGLTPVSEPRLLEILKEVANLKSCAGVLLGYDMFCY NPLLETITRTTLDTVMYSCSKIP LMGDTAHHLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCYRLGASLDYGMNLSIYNNNSLNLFFCIDLGATDFDRAQRIAHKAYMYNLSNILLVK QLFSRDVTLALDVTEPQEIYDRL KAYTSKNMKRAEEYLTAHPEIIVID*

SEQ ID No. 186 - Pretorisuskop/96/4 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQIFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVHLKRLCTELGGLTPVTEPRLLEILKEVAKLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYKRHKNHLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLQKKNYAA IYYCYRLGASLDYGMNLSIYNNNTLNMFFCIDLGATDFDRAQHIAHKAYMYNLSNIFLVK QLFSRDVTLALDVTEPQEIYDRL KSYTSKNLKRAEEYLTAHPEIIVID*

SEQ ID No. 187 - Mkuzi 1979 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVANLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYKRHKNQLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNMKRAEEYLTAHPEIIVID*

SEQ ID No. 188 - L60 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVANLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYKRHKNQLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNMKRAEEYLTAHPEIIVID*

SEQ ID No. 8 - Benin 97/1 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNNCKQVFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVYLKRLCAELGGLTPVSEPRLLEILKEVANLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVTYTCSNIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYKRHKNQLYWRVACSLYFNNIFDIHELCRE KEIClSPNLMMKFACLREKNYAA IYYCHRLGASLDYGMNLSIYNNNTLNMFFCIDLGAADFDRAQLIAHKAYMYNLSNIFLVK QLFSRDVTLVLDVTEPQEIYDML KTYTSKNMKRAEEYLTAHPEIIVID*

SEQ ID No. 189 - Malawi Lil-20/1 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNTCKQIFSYKHLQCFSED DLCLEAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVHLKRLCMELGGLTPVSESRLLEILKEVADLKSCAGVLLGYDMFCH NPLLETVTRTTLDTVMYTRSKIP LTGDTAHLLLSKFWFALALRHNFTKAIHYFYEKHKNQLYWRLTCSLYFNNIFDLHELCCK KEIClSPNLMMKFACLREENYAA IYYCHMLGASLDYGMNLSIYNNNTLNLFFCIDLGATNFDRARLIARRVYMYNLSNLFLVK QLFSRDVSLILDLTEPQAIYDML NTYTSKNLKQAEEYFTAHPEIW ID*

SEQ ID No. 190 - Kenya 1950 MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNTCKQIFSYQHLQCFSED DLCLDAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVDLKRLCTELGGLTPVSESRLLEILKEVADLKSCAGVLLGYDMFCH NPLLETVTRTTLNTVMYTRSKIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYEKHKNQLYWRLACSLYFNNIFDLHELCSK KEIClSPNLMMKFACLREENYAA IYYCHMLGASPDYGMNLSIYNNNTLNLFFCIDLGATNFDRARLIAHRVYMYNLSNIFLVK QLFSRDASLVLNITEPQAIYDML KTYTSKNLKRAEEYFTAHPEIW ID*

SEQ ID No. 191 - Ken06.Bus MGF 360 14L protein

MLSLQTLAKKWACNYLSSDYDYTLQRFGLWWDLGPIHLCNTCKQIFSYQHLQCFSVD DLCLDAALVKAVKSDNLELIRLFVD WGANPEYGLIRVPAVHLKRLCTELGGLTPVSESRLLEILKEVADLKSCAGVLLGYDMFCH NPLMETVTRTTLNTVMYTRSKIP LTGDTAHLLLTKFWFALALRHNFTKAIHYFYEKHKNQLYWRLTCSLYFNNIFDLHELCSK KEIClSPNLMMKFACLREENYAA IYYCHMLGASPDYGMNLSIYNNNTLNLFFCIDLGATNFDRARLIAHRVYMYNLSNIFLVK QLFSRDASLVLNITEPQAIYDML KTYTSKNLKRAEEYFTAHPEIW ID*

MGF 5051R protein sequences

SEQ ID No. 192 - Georgia 2007/1 MGF 505 1R protein

MFSLQNLCRKTLPNRKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRTAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRQCFFDCILYQAVKY SKFRVLLYFKHRLEDDLPFTHLL IEKACKDHNYEVIKWIYENLHIYNMIDTFECAIAHKDLHLYCLGYRFIYNRIVPDKYHHL DIRMLSSLQLLHKVAAKGYLDFI LETLKYDHNKDNINIILTQAATYNHRKILIYFIPQSTHAQIEQCLLVAIKAKSSRKTLNL LLSHLNLSINLIKKISHYVATYN STNIIGILSMRRKKKIYLDIILTKFVKKAIFNKFW RCMDTFSINPERILKIAARINRMMLVKKISEHVWKNHAVRLKYLKHA VHTMKHKDGKNRLMNFIYDRCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFVMGVLSKKEILQDYPSIYSKQYMP*

SEQ ID No. 193 - China/2018/AnhuiXCGQ MGF 505 1R protein

MFSLQNLCRKTLPNRKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRTAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRQCFFDCILYQAVKY SKFRVLLYFKHRLEDDLPFTHLL IEKACKDHNYEVIKWIYENLHIYNMIDTFECAIAHKDLHLYCLGYRFIYNRIVPDKYHHL DIRMLSSLQLLHKVAAKGYLDFI LETLKYDHNKDNINIILTQAATYNHRKILIYFIPQSTHAQIEQCLLVAIKAKSSRKTLNL LLSHLNLSINLIKKISHYVATYN STNIIGILSMRRKKKIYLDIILTKFVKKAIFNKFW RCMDTFSINPERILKIAARINRMMLVKKISEHVWKNHAVRLKYLKHA VHTMKHKDGKNRLMNFIYDRCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFVMGVLSKKEILQDYPSIYSKQYMP*

SEQ ID No. 194 - Tengani 62 MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQHNLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRQCFFDCILYQAVKY SKFRVLLSFKHRLRDDLPFTHLL IEKACKDHNYEVIKWIYENLHIYNMIDTFECAIAHKDLRLYCLGYRFIYNRIVPDKYHHL DIRMLSSLQLLHKVAAKGYLDFI LETLKYDHNKDNINIILTQAATYNHRKILIYFIPQSTHAQIEQCLLVAIKTKSSKKTLNL LLSHLNLSINLIKKISHYVATYN STNIIGILSMRRKKKIYLDIILTKFVKKAIFNKFW RCMDTFSINPERILKIAARINRTMLVKKISEHVWKNHAVRLKYLKHA VHTMKHKDGKNRLMNFIYDRCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFVMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 195 - Warmbaths MGF 505 1 R protein MFSLQNLCRKTLPDCKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQRTLIPVNEA LRIAASEENYEIVGLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIIRRCFFNCILYQAVKY SKFRVLLYFKHRLEDDLPLTHLL IEKACEDHNYEVIKWIYENLHTYDIMDTFECAIAHKDLRLYCLGYTFIYNRIVPYEYHHL DILILSSLQLLHKVAAKGYLDFI LETLKYDHNNDNLDIILTQAATYNHRKILTYFIPQLTYAQIEQCLFMAIKRKSSKKTLNL LLSHLTLSIELIKKISQYWTYN STNIIGILSMKRKKKIYLDIMLTKYVKYAIFNKYW RCMDRFSINPERIIKMAARINRMMLVKKISEHVWKNHAARLKHLKHA VHTMKHKDGKNRLMNFIYDRCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIQF KSLLLDCSHIIGKNAHDATNINI VNKYIGDLFAMGVLSKKEILQDYPSIYSKHYML*

SEQ ID No. 196 - Mkuzi1979 MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDDYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPI NEALRTAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYRLEDDLPLTHLL IEKACENHNYEVIRWIYENLHIYNMIDTFECAIAHKDLRLYCLGYTFIYNRIVPNKYHHI DILILSSLQLLHKVAAKGYLDFI LETLKYDHNNDNLDIILTQAATYNHRKILTYFIPQSTYAQIEQCLMVAIKTKSSKKTLNL LLSHLNLSIKLIKKISQYWTYN STNIIGILSMKRKKKIYLDILLTKFVKNAIFNKFW RYMDTFSINPEKIVKMAARINKMMLVKKISEHIWKNHAARLEHLKHA VHTMKHKDGKNRLMNFIYEYCYYHMQGEEIFSLARFYAIHHAPKLFDVFYNCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIDNLFAMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 197 - Warthog MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYRLENDLPLAHLL IKKACEDHNYEVIKWIYENLHIYNIMDTFGCAIAHKDLRLYRLGYTFIYNRIVPYKYHYL DVLILSGLHLLYKVAAKGYLDFI LETLKYDHNNDNLDIILTQAATYNHRKILTYYIPQLTYAQIEQCLFMAIKKKSSKKTLNL LLSHLKLSIKLIKKISQYVATYN STNIIGILNMRRKKKIYLDIILTKFVKKAIFNKFW RCMDTFSINPERIIKMAARINKMLLVKKISEHAWKNHAARLKHLKHA VYTMKHKDGKNRLMNLIYDHYYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCLLDTIRF KNLLLDCSHIIGKNAHDATNITI VNKYIGNLFAMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 198 - L60 MGF 505 1 R protein

MFSLQNLCRKTLPDCKLPEFFDDYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRIAASEENYEIVGLLLAWEGNL YYAIIGALEGNRYNLIRKYDDQIKDHHDILPFIDDPIIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYTLEDDLPLVHLL IEKACEDHNYEVIKWIYENLHVCHIIDTFDCAIAHKDLRLYCLGYTFIYNRIVPYKYHHL DILILSSLQLLHKVAAKGYLDFI LETLKYDHNIDNLDVILTQAATYNHRKILTYFIPQSTYAQIEQCLFVAIKTKSSKKTLNL LLSHLNLSIKLIQKISQYVATFN STNIIGILSMKRKKKIYLDIILTKFVKNAIFNKFW RCMERFSINPERIVKMAARINKMMLVKKISEHVWKNHAARLKHLKHA VHTMKHKDGKNRLMNFIYEHCYYHMQGEEIFSLARFYAIHHAPKLFDVFYNCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFAMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 9 - Benin 97/1 MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDDYILQLLGLYWENHGTIQRAGNNCVLIQQHTLIPV NEALRIAASEENYEIVGLLLAWEGNL YYAIIGALEGNRYNLIRKYDDQIKDHHDILPFIDDPIIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYTLEDDLPLVHLL IEKACEDHNYEVIKWIYENLHVCHIIDTFDCAIAHKDLRLYCLGYTFIYNRIVPYKYHHL DILILSSLQLLHKVAAKGYLDFI LETLKYDHNIDNLDVILTQAATYNHRKILTYFIPQSTYAQIEQCLFVAIKTKSSKKTLNL LLSHLNLSIKLIQKISQYVATFN STNIIGILSMKRKKKIYLDIILTKFVKNAIFNKFW RCMERFSINPERIVKMAARINKMMLVKKISEHVWKNHAARLKHLKHA VHTMKHKDGKNRLMNFIYEHCYYHMQGEEIFSLARFYAIHHAPKLFDVFYNCCILDTIRF KSLLLDCSHIIGKNAHDATNINI VNKYIGNLFAMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 199 - Pretorisuskop/96/4 MGF 505 1 R protein

MFSLQNLCRKTLPDCKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLVQQHTLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHDLIRKYDDQIKDHHEILPFIDNPVIFHKCHIMRRCFFDCILYQAVKY SKFRVLLYFKYRLENDLPLAHLL VEKACEDHNYEVIKWLYENLHIYNIMETFECAIAHKDLRLYRLGYTFIYNRIVPYKYHYL DVLILSGLHLLYKVAAKGYLDFI LETLKYDHNNDNLDIILTQAVTYNHRKILTYYIPQLTYAQIEQCLFMAIKKKSSKKTLNL LLSHLKLSIKLIKKISQYVATYN STNIIGILNMKRKKKIYLDIILTKFVKYAIFNKYW RCMDTFSINPERIIKMAARINKMLLVKKISQHAWKNHAARLKHLKHA VYTMKHKDGKNRLMNLIYDHYYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCLLDTIRF KSLLLDCSHIIGKNAHDATNITI VNKYIGNLFAMGVLSKKEILQDYPSIYSKHYMP*

SEQ ID No. 200 - Kenya 1950 MGF 505 1R protein

MFSLQNLCRKTLPDRKLPEFFDDYVLQLLGLYWENHGTIQRAGNNCVLIQQHNLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHNLIRKYDDQIKDHHEILPFIDDPVIFHKCHMMRRCFFNCILYQAVKY SKFSVLLYFKYILKENLPLVHSL IEKACEDHNYEVIKWIYENLHIYEIMDTFKCAIAHKDLHLYSLGYTFIYNRIVPYKYHHL DIRILSRLQLLHKVTAKGYLDFI LETLKYDHNKDNINIILTQAATYNHRNILTYFIPQSTYAQIEQCLFVAIKTNASKKTLNL LLSHLNLSIKLVKKLSQYWAYK STNIISILSMQQKKKIYLDIILTKW KNAIFIKFVIGCMVTFSINPERIVKMAARIKKMKLVKNISEHW KNHAAKLKHLKHA VHTMKHQEGKNRLMNFIYDHCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSYIIAKNAHDASINIV NKYIGNLFAMGVLSKKEILQDYPSIYSKDYML*

SEQ ID No. 201 - Ken06.Bus MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDDYVLQLLGLYWENHGTIQRAGNNCVLIQQHNLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNHHNLIRKYDDQIKDHHEILPFIDDPVIFHKCHTMRRCFFNCILYQAVKY SKFSVLLYFKYILKENLPLVHSL IEKAYKYHNYEVIKWIYENLHIYDIINTFKCAIAHKDLRLYCLGYTFVYNRIVPYKYYHL DIRILLRLQLLHKVTAKGYLDFI LETLKYDHNTNNIDIILTQAATYNHRNILTYFIPQSTYAQIEQCLFVAIKTNASKKTLNL LLSHLNLSIKLVKKLSQYWAYK

STNIISILSRQQKKKIYLDIILTKW KNAVFNKFVIGCMVTFSINPERIVKMAARIKKMKLVKNISEHVWKNHAVKLKYLKHA VHTMKHQEGKNRLMNFIYDHCYYHMQEEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSHIIVKNAHDASINIV NKYIGNLFAMGVLSKKEILQDYPSIYSKDYML* SEQ ID No. 202 - Ken05/Tk1 MGF 505 1R protein

MFSLQNLCRKTLPDCKLPEFFDDYVLQLLGLYWENHGTIQRAGNNCVLIQQHNLIPV NEALRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRHNLIRKYDDQIKDHHEILPFIDDPVIFHKCHIMRRCFFNCMLYQAVKY SKFSVLLYFKYILKENLPLVHSL IEKAYKYHNYEVIKWIYENLHIYDIINTFKYAIAHKDLRLYCLGYTFVYNRIVPYKYYHL DIRILLRLQLLHKVTAKGYLDFI LETLKYDHNTNNIDIILTQAATYNHRNILTYFIPQSTYAQIEQCLFVAIKTNASKKTLNL LLSHLNLSIKLVKKLSQYWAYK STNIISILSMQQKKKIYLDIILTKWKNAVFIKFVIGCMVTFSINPERIVKMAARIKKMKL VKNISEHVWKNHAAKLKHLKHA VHTMKHQEGKNRLMNFIYDHCYYHMQGEEIFSLARFYAIHHAPKLFDVFYDCCILDTIRF KSLLLDCSHIIAKNAHDASINIV NKYIGNLFAMGVLSKKEILQDYPSIYSKYDIL*

SEQ ID No. 203 - Malawi Lil-20/1 (1983) MGF 505 1R protein MFSLQNLCRKTLPDCKLPEFFDEYILQLLGLYWENHGTIQRAGNNCVLIQQHNLIPVNEA LRIAASEENYEIVSLLLAWEGNL YYAIIGALEGNRPDLIRKYDDQIKDHHEILPFIDDPIIFHKCHIMRRCFFNCILYQAVKY SKFRVLLYFKHRLGDDLPLTHLL IEKACEDHNYEVIKWIYENLHSYNIMDTFECAIAHKDLRLYCLGYTFIYNRIVPYKYHHL DICILSSLQLLHKVAAKGYLDFI LETLKYDHNINNIDIILTQAATYNHRKILTYFIPQLTYAQIEQCLLVAIKTKASKKTLNL LLSHLNLSIKLIKKISQYWTYN STNIISILSMRRKKKIYLDIILTEFVKNAIFNKFW RCMDTFSINPERIVKMAARINRMMLVKNISERVWKNHAVKLKHLKHA VHTMKHQEGKNRLMNFIYDHCYYHMQGEEIFGLARFYAIHHAPKLFDVFYDCCMLDATRF KSLLLDCPHIIGKNAYDAGINLV NKYIGNLFAMGVLSKKEILQDYPSIYSKHDMF*

DP148R

The DP148R gene is located close to the right end of the ASFV genome, at position 177915 to 178679 on the Benin 97/1 genome. The DP148R gene may also be referred to as MGF 360 18R. DP148R is expressed at early times post-infection. The amino acid sequence of the DP148R protein has no significant similarity to other proteins; the secondary structure is predicted to be predominantly helical, but no signal peptide or transmembrane domains are evident. DP148R inhibits type I interferon. DP148R also inhibits activation of the NF-kB transcription factor (see Figure 7). DP148R inhibits nuclear translocation of the p65 subunit of NF-kB (see Figure 8). NF-kB controls expression of interferon and pro-inflammatory cytokines as part of the host’s innate immune system response to viral infection. The inhibition of NF-kB by DP148R would results in decreased amounts of type I interferon (IFN) and pro-inflammatory cytokines and chemokines produced by cells infected with ASFV and allow ASFV to circumvent the host innate immune response, favouring virus replication and disrupting the development of adaptive responses. The gene (i.e. nucleotide) sequences and positions in the genome of DP148R genes from different ASFV strains are presented below.

SEQ ID No. 287 - Georgia 2008/1 DP148R (MH910495.1 : 183350-184063)

ATGTTAGAAATAGTATTGGCAACGCTGCTAGGCGACCTGCAGCGGCTCCGGGTTCTT ACCCCTCAGCAGCGGGCAGTTGCCTT

CTTTCGAGCCAATACTAAGGAGCTAGAGGACTTCTTATGCTCAGATGGGCAGTCTGA GGAGGTACTGTCTGGCCCCCTTCTTA

ACCGTCTACTAGAACCCTCAGGCCCTCTTGATATTTTAACCGGATATCACCTATTTC GTCAGAATCCCAAGGCAGGTCAGTTG

CGCGGCCTTGAGGTCAAGATGCTTGAACGGTTATACGATGCTAATATTTACAATATA CTGTCTCGGCTGCGGCCTGAAAAAGT

TCGCAACAAGGCTATTGAGCTATACTGGGTTTTCCGAGCTATCCATATTTGTCATGC TCCTTTAGTTTTAGATATTGTACGAT

ATGAGGAACCGGACTTTGCTGAACTGGCCTTTATTTGTGCTGCTTACTTTGGTGAAC CTCAGGTAATGTATTTGCTCTACAAA

TATATGCCTCTGACCCGCGCAGTTCTTACGGATGCCATCCGGATAAGTCTTGAGAGC AACAACCAGGTAGGGATTTGCTATGC

TTACTTGATGGGAGGCAGCCTCAAGGGACTAGTCTCCGCCCCACTGCGTAAACGTCT GCGCGCCAAACTACGCTCGCAGCGCA

AAAAGAAGGACGTTCTTTCACCCCACGACTTCTTACTGCTGCTCCAGTAG

SEQ ID No. 288 - China/2018/AnhuiXCGQ DP148R (MK128995.1 :183372-184085)

ATGTTAGAAATAGTATTGGCAACGCTGCTAGGCGACCTGCAGCGGCTCCGGGTTCTT ACCCCTCAGCAGCGGGCAGTTGCCTT

CTTTCGAGCCAATACTAAGGAGCTAGAGGACTTCTTATGCTCAGATGGGCAGTCTGA GGAGGTACTGTCTGGCCCCCTTCTTA

ACCGTCTACTAGAACCCTCAGGCCCTCTTGATATTTTAACCGGATATCACCTATTTC GTCAGAATCCCAAGGCAGGTCAGTTG

CGCGGCCTTGAGGTCAAGATGCTTGAACGGTTATACGATGCTAATATTTACAATATA CTGTCTCGGCTGCGGCCTGAAAAAGT

TCGCAACAAGGCTATTGAGCTATACTGGGTTTTCCGAGCTATCCATATTTGTCATGC TCCTTTAGTTTTAGATATTGTACGAT

ATGAGGAACCGGACTTTGCTGAACTGGCCTTTATTTGTGCTGCTTACTTTGGTGAAC CTCAGGTAATGTATTTGCTCTACAAA

TATATGCCTCTGACCCGCGCAGTTCTTACGGATGCCATCCGGATAAGTCTTGAGAGC AACAACCAGGTAGGGATTTGCTATGC

TTACTTGATGGGAGGCAGCCTCAAGGGACTAGTCTCCGCCCCACTGCGTAAACGTCT GCGCGCCAAACTACGCTCGCAGCGCA

AAAAGAAGGACGTTCTTTCACCCCACGACTTCTTACTGCTGCTCCAGTAG

SEQ ID No. 289 - OURT 88/3 DP148R (NC_044957.1:168827-169045)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAGGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

CGACCTGCAGCGGCTCCGGGTTCTTACCCCTCAGCAGCGGGCGGTTGCCTTCTTTCG AGCCAATACTAAGGAGCTAGAGGACT

TCTTACGCTCAGATGGGCAATCTGAGGAGATACTGTCTGGCCCCCCTCCTTAA

SEQ ID No. 290 - L60 DP148R (NC_044941.1 : 177973-178737)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAGGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

CGACCTGCAGCGGCTCCGGGTTCTTACCCCTCAGCAGCGGGCGGTTGCCTTCTTTCG AGCCAATACTAAGGAGCTAGAGGACT

TCTTACGCTCAGATGGGCAATCTGAGGAGATACTGTCTGGCCCCCTCCTTAACCGTC TACTAGAACCCTCATGCCCTCTTGAT

ATTTTAACCGGATATCACCTATTTCGTCAGAATCCCAAGGCAGGTCAGTTGCGCGGC CTTGAGGTCAAGATGCTTGAACGGTT

ATACGATGCTAATATTTACAATATATTGTCTCGGCTGCGACCTGAAAAAGTCCGCAA CAAGGCTATTGAGCTATACTGGGTTT

TCCGAGCTATCCATATTTGTCATGCTCCTTTAGTTTTAGATATTGTACGATATGAGG AACCGGACTTTGCTGAACTGGCCTTT

ATTTGTGCTGCTTACTTTGGTGAACCTCAGGTAATGTATTTGCTCTACAAATATATG CCTCTGACCCGCGCAGTTCTTACGGA

TGCCATCCAGATAAGTCTTGAGAGCAACAACCAGGTAGGGATTTGCTATGCTTACTT GATGGGAGGCAGCCTCAAGGGACTAG

TCTCCGCCCCACTGCGTAAACGTCTGCGCGCCAAACTACGCTCGCAGCGCAAAAAGA AGGACGTTCTTTCACCCCACGACTTC

TTACTGCTGCTCCAGTAG

SEQ ID No. 291 - Benin 97/1 DP148R (NC_044956.1 :177915-178679)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAGGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

CGACCTGCAGCGGCTCCGGGTTCTTACCCCTCAGCAGCGGGCGGTTGCCTTCTTTCG AGCCAATACTAAGGAGCTAGAGGACT

TCTTACGCTCAGATGGGCAATCTGAGGAGATACTGTCTGGCCCCCTCCTTAACCGTC TACTAGAACCCTCATGCCCTCTTGAT

ATTTTAACCGGATATCACCTATTTCGTCAGAATCCCAAGGCAGGTCAGTTGCGCGGC CTTGAGGTCAAGATGCTTGAACGGTT

ATACGATGCTAATATTTACAATATATTGTCTCGGCTGCGACCTAAAAAAGTCCGCAA CAAGGCTATTGAGCTATACTGGGTTT

TCCGAGCTATCCATATTTGTCATGCTCCTTTAGTTTTAGATATTGTACGATATGAGG AACCGGACTTTGCTGAACTGGCCTTT

ATTTGTGCTGCTTACTTTGGTGAACCTCAGGTAATGTATTTGCTCTACAAATATATG CCTCTGACCCGCGCAGTTCTTACGGA

TGCCATCCAGATAAGTCTTGAGAGCAACAACCAGGTAGGGATTTGCTATGCTTACTT GATGGGAGGCAGCCTCAAGGGACTAG

TCTCCGCCCCACTGCGTAAACGTCTGCGCGCCAAACTACGCTCGCAGCGCAAAAAGA AGGACGTTCTTTCACCCCACGACTTC

TTACTGCTGCTCCAGTAG

SEQ ID No. 292 - Warthog DP148R (AY261366.1:180836-181548) ATGTTAGAAATAGTATTGGCAACGCTGCTAGGTGACCTGCAGCGGCTCCGGGTTCTTACC CCTCAGCAGCGGGCGGTTGCCTT

CTTTCGAGCCAATACTAAGGAGCTAGAGGACTTTTTATGCTCAGATGGGCAGTCTGA GGAGATACTGTCCGGCCCCCTCCTTA

ACCGTCTACTAGAACCCTCAGGCCCTCTTGATATTTTAACCGGATATCACTTATTTC GTCAGAATCCCAAGGCAGGTCAGGTG

CGCGGCCTTGAGGTCAAGATGCTTGAACGGTTATACGATGCTAATATTTACAATATA TTGTCTCGGCTGCGACCTGAAAAAGT

TCGCAACAAGGCTGTTGAGCTATACTGGGTTTTTCGGGCTATCAATATGTGTCATGC TCCTTTAGTTTTAGATATTGTACGAT

ATGAGGAACCGGACTTTGCTGAACTGGCCTTTATTTGTGCTGCTTACTTTGGTGAAC CTCAGGTAATGTATTTGCTCTACAAA

TATATGCCTCTGACCCGCGCAGTTCTTACGGATGCCATCCAGATAAGTCTTGAGAGC AACAGCCAGGTAGGGATTTGCTATGC

CTACTTGATGGGAGGCAGCCTCAAGGGCCTAGTCCGTGCCCCACTACGTAAACGTCT GCGCGCCAAACTACGCTCGCAGCGCA

AAAAGAAGGACGTTCTTCCACCCCATGACTTCTTACTGCTGCTCCAGTAA

SEQ ID No. 293 - Pretorisuskop/96/4 DP148R (AY261363.1 :185149-185861)

ATGTTAGAAATAGTATTGGCAACGCTGCTAGGTGACCTGCAGCGGCTCCGGGTTCTT ACCCCTCAGCAGCGGGCGGTTGCCTT

CTTTCGAGCCAATACTAAGGAGGTAGAAGACTTCTTATGCTCAGATGGGCAGTCTGA GGAGGTACTGTCCGGCCCCCTCCTTA

ACCGTCTACTAGAACCCTCAGGCCCTCTTGATATTTTAACCGGATATCACTTATTTC GTCAGAATCCCAAGGCGGGTCAGGTG

CGCGGCCTTGAGGTCAAGATGCTTGAACGGTTATACGATGCTAATATTTACAATATA TTGTCTCGGCTGCGACCTGAAAAAGT

TCGCAACAAGGCTGTTGAGCTATACTGGGTTTTTCGGGCTATCAATATGTGTCATGC TCCTTTAGTTTTAGATATTGTACGAT

ATGAGGAACCGGACTTTGCTGAACTGGCCTTTATTTGTGCTGCTTACTTTGGTGAAC CTCAGGTAATGTATTTGCTCTACAAA

TATATGCCTCTGAGCCGCGCAGTTCTTACGGATGCCATCCAGATAAGTCTTGAGAGC AACAGCCAGGTAGGGATTTGCTATGC

CTACTTGATGGGAGGCAGCCTCAAGGGCCTAGTCCGTGCCCCACTACGTAAACGTCT GCGCGCCAAACTACGCTCGCAGCGCA

AAAAGAAGGACGTTCTTCCACCCCACGACTTCTTACTGCTGCTCCAGTAA

SEQ ID No. 294 - Tengani 62 DP148R (AY261364.1:179845-180557)

ATGTTAGAAATAGTATTGGCAACGCTGCTAGGTGACCTGCAGCGGCTCCGGGTTCTT ACCCCTCAGCAGCGGGCGGTTGCCTT

CTTTCGAGCCAATACTAAAGAGCTAGAGGACTTCTTATGTCCAGATGGGCAGTCTGA GGAGGTGCTGTCTGGCTCCCTCCTTA

ACCGTCTACTAGAACCCTCAGGCCCTCTTGATATTTTAACTGGATATCACCTATTTC GTCAGAATCCCAAGGCAGGTCAGTTG

CGCGGCCTTGAGGTCAAGATGCTTGAACGGTTATACGATGCTAATATTTACAACATA TTGTCCCGGCTGCGACCTGAAAAAGT

TCGCAACAAGGCTGTTGAGCTATACTGGGTTTTTCGGGCTATCAATATGTGTCATGC TCCTTTAGTTTTAGATATTGTACGAA

ATGAGGAACTGGACTTTGCTGAACTGGCCTTTATTTGTGCTGCTTACTTTGGTGAGC CTCAGGTAATGTATTTGCTCTACAAA

TATATGCCTCTGACCCGCGCAGTTCTTACGGATGCCATCCAGATAAGTCTTGAGAGC AACAGCCAGGTGGGGATTTGCTATGC

CTACTTGATGGGAGGCAGCCTCAAGGGACTAGTCCGTGCCCCGCTACGTAAACGTCT GCGCGCCAAACTACGCTCGCAGCGCA

AAAAGAAGGATGTTCTTCCACCCCACGACTTCTTACTGCTGCTCCAGTAA

SEQ ID No. 295 - Ken06.Bus DP148R (NC_044946.1 :181097-181870)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAAGATGTTA GAAATAGTATTGGCAACGCTGCTGGG

TGATCTGCAGCAACTTAAGGATCTTACACCTAAGCAGCGGGCCGTAGCTTTCTTCCG AGCCAACACCAAAGAGCTAGAGGACT

TTCTCTACCCCGATGGGCAGACTGAGGAGTTACTCCCTGGATTTCTTCTTAACCATT TACTAGAACCCTCAGGCCCTATTGAA

ATTTTAACCGGATATCACCTCTTTCGTCAGAATCCAAAGGCAGGTCGCTTGCGAGGC CTTGAGGTGAAAATGCTTGAACGGTT

ATATGATGCTAATATTTACAATATGCTGGCTCGGCTACGGCCTGAGTTGGTTCGTGA CAAGGCTGTTGAGCTATATTGGCTTT

TTCGAGCTATTTTAATATGTCATGGTCCCTTGGTTTTGGAGATTGTACGACATGAGA CGTTGGACTTTGCAGAAACCGCCTTT

ATCTGTGCTGCTTACTTTAGTGAACCTCAGGTAATGTACGCTCTTTATAACTTTATA CCACCCACTCATGCAGTCCTTGCTGA

TGCCATCCAGATGTGTCTTGAGAGCAACAGCGAGGCAGGGATTTGCTATGTCTACCT AATGGGAGGTAACCTCAAGGGCAAGG

TGCCCGGCTCGCTGCGCAAACGTCTGCGTGCCAGTCCACTTCGGCAAGAACGCAAAA AGAAAAACGTCCTTCCGCCCCACGAA

TTCCTACTCCTGCTCCACGGGATTTAA

SEQ ID No. 296 - Kenya 1950 DP148R (AY261360.1 :189099-189872)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAAGATGTTA GAAATAGTATTGGCCACGCTGCTAGG

TGATCTGCAGAAACTTAAGGATCTTACACCTCCGCAGCGGGCTGTGGCTTTCTTTCG AGCCAACACCAAAGAGCTAGAGGACT

TTCTCTACCCCGATGGGCAGTCTGAGGAGTTACTCCCTGGACTTCTCCTTAACCGTT TACTAGAACCTTCAGGTTCTATTGAC

ATTTTAACCGGTTACCACCTATTTCGTGAGAATCCAAAGGCAGGTCGCTTGCGAGGC CTTGAGGTGAAAATGCTTGAACGGTT

ATATGATGCTAATATTTACAATATGCTGGCTCGGCTACGGCCTGAGTTGGTTCGTGA CAAGGCTATTGAGCTATATTGGCTCT

TTCGGGCTATTTTAATGTGTCATAGTCCCTTAGTTTTGGAGATTGTACGACATGAGA CAATGGATTTTGCAGAAACCGCCTTT

ATCTGTGCCGCTTACTTTAGTGAACCTCAGGTAATGTACGCTCTTTATAAATTTATA CCTATCTCTCGTGCAGTCCTTGCTGA

TGCCATTCAGATGTGTCTTGAGAGCAACAGCGAGGCGGGGATTTGCTATGCTTACCT AATGGGGGGTAGCCTCAAGGGCAAGG

TGCCTGGCTCGCTGCGCAAACGTCTGCGTGCCAGTCCACTTCGGCAAGAGCGCAAAA AGAAAAACGTCCTCCCGCCCCATGAA

TTCCTACTCATGCTCCATGGGATTTAA

SEQ ID No. 297 - Warmbaths DP148R (AY261365.1:184288- 185048)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAGGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

TGACCTGCAGCGGCTCCGGGTTCTTACCCCTCAGCAGCGGGCGGTTGCCTTCTTTCG AGCCAATACTAAGGAGCTAGAGGACT TCTTATGCTCAGATGGGCAGTCTGAGGAGATACTGTCCGGCCCCCTCCTTAACCGTCTAC TAGAACCCTCAGGCCCTCTTGAT

ATTTTAACCGGATATCACTTATTTCGTCAGAATCCCAAGGCAGGTCAGTTGCGCGGT CTTGAGGTCAAGATGCTTGAACGGTT

ATACGATGCTAATATTTACAATATATTGTCTCGGCTGCGACCTGAAAAAGTTCGCAA CAAGGCTATTGAGCTATACTGGGTTT

TCCGAGCTATCCATATTTGTCATGCTCCTTTAGTTTTAGATATTGTACGATATGAGG AACCGGACTTTGCTGAACTGGCCTTT

ATTTGTGCTGCTTACTTTGGTGAACCTCAGGTAATGTATTTGCTCTACAAATATATG CCTCTGACCCGCGCAGTTCTTACGGA

TGCCATCCGGATAAGTCTTGAGAGTAACAACCAGGTAGGGATTTGCTATGCTTACTT GATGGGAGGCAGCCTCAAGGGACTAG

TCTCCGCCCCACTGCGTAAACGTCTGCGCGCCAAACTACGCTCACAGCGCAAAAAGA AAGACGTTCTTTCACCCCACGACTTC

TTACTGCTGCTCCAGTAA

SEQ ID No. 298 - Mkuzi 1979 DP148R (AY261362.1:185751-186511)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAGGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

CGACCTGCAGCGGCTCCGGGTTCTTACCCCTCAGCAGCGGGCGGTTGCCTTCTTTCG AGCCAATACTAAGGAGCTAGAGGACT

TCTTATGCTCAGATGGGCAGTCTGAGGAGATACTATCTGGCCCCCTTCTTAACCGTC TACTAGAACCCTCAGGCCCTCTTGAT

ATTTTAACTGGATATCACCTATTTCGTCAGAACCCCAAGGCAGGTCAGTTACGCGGC CTTGAGGTCAAGATGCTTGAACGGTT

ATACGATGCTAATATTTACAATATATTGTCTCGGCTGCGACCTGAAAAAGTTCGCAA CAAGGCTATTGAGCTTTACTGGGTTT

TTCGAGCTATCCATATTTGTCATGCTCCTTTAGTTTTAGATATTGTACGATATGAGG AACCGGACTTTGCTGAACTGGCCTTT

ATTTGTGCTGCTTACTTTGGTGAACCTCAGGTAATGTATTTGCTCTACAAATATATG CCTCTGACCCGCGCAGTTCTTACGGA

TGCCATCCGGATAAGTCTTGAGAGTAACAACCAGGTAGGGATTTGCTATGCTTACTT GATGGGAGGCAGCCTCAAGGGACTAG

TCTCCGCCCCACTGCGTAAACGTCTGTGCGCCAAACTACGCTCGCAGCGCAAAAAGA AGGACGTTCTTTCACCCCACGACTTC

TTACTGCTGCTCCAGTAA

SEQ ID No. 299 - Ken05/Tk1 DP148R (NC_044945.1:186074-186847)

ATGCAAAATAAAATCCCAAATTTTAACCTTTTCTTTTTTTTTCTATACAAGATGTTA GAAATAGTATTGGCAACGCTGCTAGG

TGATCTGCAGAAACTTAAAGATCTTACACCTATGCAGCGGGCTGTGGCTTTCTTTCG AGCCAACACTAAAGAGCTAGAGGACT

TCCTCTGCCCCGATGGGCAGTCTGAGGAGTTACTCCCTGGACTTCTCCTTAACCGTT TATTAGAACCTTCAGGTCCTATTGAC

ATTTTAACCGGTTACCACCTATTTCGTGAGAATCCGAAGGCAGGTCGGCTGCGTGGC CTTGAGGTCAAGCTGCTTGAACGGTT

ATATGATGCTAACATCTACAATATATTGGCCCAGCTACGGCCTGAGTTGGTTCGCAA TAAGGCTGTTGAGTTATATTGGCTCT

TTCGGGCTATTTTGATGTGTCATGGTCCCTTAGTTTTGGAGATTGTACGACATGAGA CAATGGATTTTGCAGAATTAGCCTTT

ATCTGTGCTGCTTACTTTAGTGAACCTCAGGTAATGTACGCTATTTATAAATTTATA CCTATCTCTTGCGCAGTCCTTGCTGA

TGCCATTCAGATGTGTCTTGAGAGTAACAGCGAGGCGGGGATTTGCTATGCTTACCT AATGGGGGGTAGCCTCAAGGGCAAGG

TGCCTGGCGCGCTGCGCAAACGTCTGCGTGCCAGTCCACTTCGGCAAGAGCGCAAAA AGAAAAACGTTCTTCCACCCCATGAA

TTCCTACTCATGCTTCATGGGATTTAA

SEQ ID No. 300 - Malawi Lil-20/1 (1983) DP148R (AY261361.1:183573- 184346)

ATGTTAGAAATAGTATTGGCAACGCTGCTAGGTGATCTGCAGAAGCTTAGGGATCTT ACACCTATGCAGCGGGCTGTGGCTTT

CTTTCGAGTCAACACTAAAGAGCTAGAGGACTTCCTCTACCCCGATGGGCAGTCTGA GGAGTTGCTCCCTGGACTTCTCCTTA

ACCGTTTACTAGAACCTTCAGGCCCTATTGACATTTTAACCGGTTACCACCTATTTC GTGAGAACCCAAAGGCAGGTCGGCTG

CGTGGCCTTGAGGTTAAGCTGCTTGAACGGTTATATGATGCTAACATCTACAATATG TTGGCCCAAATACGACCTGAGTTGGT

TCGTATCAAGGCTATTGAGTTATATTGGCTCTTTCGGGCTATTTTAATGTGTCATAG TCCCTTAGTTTTGGAGATTGTACGAC

ATGAGACAATGGATTTTGCAGAATTAGCCTTTATCTGTGCTGCTTACTTTAGTGAAC CTCAGGTAATGTACGCTCTTTATAAA

TTTATACCTATTTCTCGCGCAGTCCTTGCTGATGCCATCGAGATGTCTCTTGAGAGC AACAGCGAGACGGGGATTTGCTATGC

CTACCTAATGGGGGGTAGCCTCAAGGGCAAGGTGCCCGGCCCGCTGCGCAAACGTCT GCGTGCCAGTCCACTTCGGCAAGAGC

GCAAAAAGAAAAACGTCCTTCCGCCCCATGAATTTCTACTCATGCTCCATGGGATTT AA

In an embodiment the invention provides an attenuated ASF virus in which the expression and/or activity of the DP148R gene is disrupted. Suitably, the DP148R gene comprises the sequence of SEQ ID No. 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299 or 300. Suitably, the DP148R gene comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299 or 300. Suitably, the DP148R gene consists of the sequence of SEQ ID No. 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299 or 300.

The amino acid sequence of the Benin 97/1 DP148R protein is depicted below as SEQ ID No. 11: SEQ ID No. 11 - Benin 97/1 DP148R protein

MQNKIPNFNLFFFFLYRMLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFL RSDGQSEEILSGPLLNRLLEPSCPLD ILTGYHLFRQNPKAGQLRGLEVKMLERLYDANIYNILSRLRPKKVRNKAIELYWVFRAIH ICHAPLVLDIVRYEEPDFAELAF ICAAYFGEPQVMYLLYKYMPLTRAVLTDAIQISLESNNQVGICYAYLMGGSLKGLVSAPL RKRLRAKLRSQRKKKDVLSPHDF LLLLQ

Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No. 11. Suitably, the DP148R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 11. Suitably, the DP148R gene encodes a protein consisting of the sequence of SEQ ID No. 11.

Orthologous DP148R sequences from other genomes share between 74 and 99% amino acid identity. The orthologous DP148R genes from other ASFV isolates are depicted below as SEQ ID Nos 12 to 19, and their location in the ASFV genome is indicated:

SEQ ID No. 12 - Warthog DP148R protein: 181103 to 181549

MLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFLCSDGQSEEILSGPLLNR LLEPSGPLDILTGYHLFRQNPKAGQV RGLEVKMLERLYDANIYNILSRLRPEKVRNKAVELYWVFRAINMCHAPLVLDIVRYEEPD FAELAFICAAYFGEPQVMYLLYK YMPLTRAVLTDAIQISLESNSQVGICYAYLMGGSLKGLVRAPLRKRLRAKLRSQRKKKDV LPPHDFLLLLQ

SEQ ID No. 13 - Kenya 1950 DP148R protein: 189417 to 189872

MQNKIPNFNLFFFFLYKMLEIVLATLLGDLQKLKDLTPPQRAVAFFRANTKELEDFL YPDGQSEELLPGLLLNRLLEPSGSID ILTGYHLFRENPKAGRLRGLEVKMLERLYDANIYNMLARLRPELVRDKAIELYWLFRAIL MCHSPLVLEIVRHETMDFAETAF ICAAYFSEPQVMYALYKFIPISRAVLADAIQMCLESNSEAGICYAYLMGGSLKGKVPGSL RKRLRASPLRQERKKKNVLPPHE FLLMLHGI

SEQ ID No. 14 - Malawi LIL20/1 DP148R protein: 183687 to 184346

MLEIVLATLLGDLQKLRDLTPMQRAVAFFRVNTKELEDFLYPDGQSEELLPGLLLNR LLEPSGPIDILTGYHLFRENPKAGRL

RGLEVKLLERLYDANIYNMLAQIRPELVRIKAIELYWLFRAILMCHSPLVLEIVRHE TMDFAELAFICAAYFSEPQVMYALYK

FIPISRAVLADAIEMSLESNSETGICYAYLMGGSLKGKVPGPLRKRLRASPLRQERK KKNVLPPHEFLLMLHGI

SEQ ID No. 15 - Mkuzi DP148R protein: 185751 to 186515

MQNKIPNFNLFFFFLYRMLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFL CSDGQSEEILSGPLLNRLLEPSGPLD ILTGYHLFRQNPKAGQLRGLEVKMLERLYDANIYNILSRLRPEKVRNKAIELYWVFRAIH ICHAPLVLDIVRYEEPDFAELAF ICAAYFGEPQVMYLLYKYMPLTRAVLTDAIRISLESNNQVGICYAYLMGGSLKGLVSAPL RKRLCAKLRSQRKKKDVLSPHDF LLLLQ

SEQ ID No. 16 - Pretorisuskop DP148R protein: 185416 to 185862

MLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKEVEDFLCSDGQSEEVLSGPLLNR LLEPSGPLDILTGYHLFRQNPKAGQV RGLEVKMLERLYDANIYNILSRLRPEKVRNKAVELYWVFRAINMCHAPLVLDIVRYEEPD FAELAFICAAYFGEPQVMYLLYK YMPLSRAVLTDAIQISLESNSQVGICYAYLMGGSLKGLVRAPLRKRLRAKLRSQRKKKDV LPPHDFLLLLQ

SEQ ID No. 17 - Tengani62 DP148R protein: 180112 to 180558

MLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFLCPDGQSEEVLSGSLLNR LLEPSGPLDILTGYHLFRQNPKAGQL RGLEVKMLERLYDANIYNILSRLRPEKVRNKAVELYWVFRAINMCHAPLVLDIVRNEELD FAELAFICAAYFGEPQVMYLLYK YMPLTRAVLTDAIQISLESNSQVGICYAYLMGGSLKGLVRAPLRKRLRAKLRSQRKKKDV LPPHDFLLLLQ

SEQ ID No. 18 - Warm baths DP148R protein: 184606 to 185052 MQNKIPNFNLFFFFLYRMLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFLCSD GQSEEILSGPLLNRLLEPSGPLD ILTGYHLFRQNPKAGQLRGLEVKMLERLYDANIYNILSRLRPEKVRNKAIELYWVFRAIH ICHAPLVLDIVRYEEPDFAELAF ICAAYFGEPQVMYLLYKYMPLTRAVLTDAIRISLESNNQVGICYAYLMGGSLKGLVSAPL RKRLRAKLRSQRKKKDVLSPHDF

LLLLQSEQ ID No. 19 - OURT88/3 DP148R protein: 169146 to 169592

MQNKIPNFNLFFFFLYRMLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFL RSDGQSEEILSGPPPSEQ I D NO.

301 - China/2018/Anhui DP148R protein

MLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFLCSDGQSEEVLSGPLLNR LLEPSGPLDILTGYHLFRQNPKAGQL RGLEVKMLERLYDANIYNILSRLRPEKVRNKAIELYWVFRAIHICHAPLVLDIVRYEEPD FAELAFICAAYFGEPQVMYLLYK YMPLTRAVLTDAIRISLESNNQVGICYAYLMGGSLKGLVSAPLRKRLRAKLRSQRKKKDV LSPHDFLLLLQ

SEQ ID No. 302 - Georgia2007/1 DP148R protein

MLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFLCSDGQSEEVLSGPLLNR LLEPSGPLDILTGYHLFRQNPKAGQL RGLEVKMLERLYDANIYNILSRLRPEKVRNKAIELYWVFRAIHICHAPLVLDIVRYEEPD FAELAFICAAYFGEPQVMYLLYK YMPLTRAVLTDAIRISLESNNQVGICYAYLMGGSLKGLVSAPLRKRLRAKLRSQRKKKDV LSPHDFLLLLQ

SEQ ID No. 303 - Ken05/Tk1 DP148R protein

MQNKIPNFNLFFFFLYKMLEIVLATLLGDLQKLKDLTPMQRAVAFFRANTKELEDFL CPDGQSEELLPGLLLNRLLEPSGPID ILTGYHLFRENPKAGRLRGLEVKLLERLYDANIYNILAQLRPELVRNKAVELYWLFRAIL MCHGPLVLEIVRHETMDFAELAF ICAAYFSEPQVMYAIYKFIPISCAVLADAIQMCLESNSEAGICYAYLMGGSLKGKVPGAL RKRLRASPLRQERKKKNVLPPHE FLLMLHGI

SEQ ID No. 304 - Ken06 DP148R protein

MQNKIPNFNLFFFFLYKMLEIVLATLLGDLQQLKDLTPKQRAVAFFRANTKELEDFL YPDGQTEELLPGFLLNHLLEPSGPIE ILTGYHLFRQNPKAGRLRGLEVKMLERLYDANIYNMLARLRPELVRDKAVELYWLFRAIL ICHGPLVLEIVRHETLDFAETAF ICAAYFSEPQVMYALYNFIPPTHAVLADAIQMCLESNSEAGICYVYLMGGNLKGKVPGSL RKRLRASPLRQERKKKNVLPPHE FLLLLHGI

SEQ ID No. 305 - L60 DP148R protein

MQNKIPNFNLFFFFLYRMLEIVLATLLGDLQRLRVLTPQQRAVAFFRANTKELEDFL RSDGQSEEILSGPLLNRLLEPSCPLD ILTGYHLFRQNPKAGQLRGLEVKMLERLYDANIYNILSRLRPEKVRNKAIELYWVFRAIH ICHAPLVLDIVRYEEPDFAELAF ICAAYFGEPQVMYLLYKYMPLTRAVLTDAIQISLESNNQVGICYAYLMGGSLKGLVSAPL RKRLRAKLRSQRKKKDVLSPHDF LLLLQ

Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No. 12. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

13. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

14. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

15. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

16. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

17. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

18. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

19. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

301. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

302. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

303. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No. 304. Suitably, the DP148R gene encodes a protein comprising the sequence of SEQ ID No.

305.

EP153R

The EP153R gene is expressed both early and late in infection. EP153R may also be referred to as 8CR. The EP153R protein is a C-type lectin. C-type animal lectins are found in serum, the extracellular matrix and cell membranes and are thought to act as receptors for carbohydrate ligands. The EP153R protein comprises a C-type lectin domain, a cell attachment sequence (RGD) and a transmembrane domain, and has similarity with CD44 molecules involved in T cell activation.

The gene (i.e. nucleotide) sequences and positions in the genome of EP153R genes from different ASFV strains are presented below.

SEQ ID No. 204 - Benin 97/1 EP153R (NC_044956.1:67030-67491)

ATGTATTTTAAGAAAAAATACAT CGGT CTTATT GATAAGAACT GT GAAAAAAAAATATTAGAT GATT CTAGTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT GATAAC T C T TAT T T AT AAT T T CAT AT T C T G G GAT AAT T AT AT AA AATGTTACCGAAATAATGATAAAATGTTTTACTGTCCTAATGATTGGGTTGGATATAATA ATATTTGTTACTATTTTAGTAAT GGTAGTTTTTCTAAAAATTATACAGCTGCTAGTAATTTTTGTAGACAATTAAATGGTACA CTTGCTAATAATGATACTAATTT ATTAAATCTAACTAAAATATATAATAATCAATCTATGTATTGGGTTAACAATACGGTAAT ATTACGTGGTGATAATAAATATA G T C AAAAAG T T AACT AT ACAGAT T TAT TAT T TAT T T G T G GT AAAT AA

SEQ ID No. 205 - Georgia 2007/1 EP153R (NC_044959.1:73369-74451)

ATGTTTTCTAACAAAAAGTACATCGGTCTTATCAATAAGAAGGAGGGTTTGAAAAAA AAAATAGATGATTATAGTATATTAAT AAT T G GAAT AT TAAT T G GAAC TAACAT CT TAAG C C T TAT TATAAATATAAT AG GAGA GAT T AATAAAC C AATAT G T T AC CAAA AT GAT GATAAGAT AT T T TAT T G C C C TAAAGAT T G G GT T G GATATAAT AAT G T T T G T TAT TAT T T T G G CAAT GAAG AAAAAAAT T AT AATAAT G C AAGT AAT TAT T G TAAG CAAT TAAATAGT AC G C T T AC TAAT AATAAT AC TAT T T T AG TAAAT C T T AC TAAAAC ATTAAATCTTACTAAAACATATAATCACGAATCTAATTATTGGGTTAATTATTCTTTAAT TAAAAATGAGTCAGTACTATTAC G T GAT AG T G GATAT T AC AAAAAACAAAAACAT G TAAG T T TAT TAT AT AT T T GT AG TAAATAA

SEQ ID No. 206 - Ken05/Tk1 EP153R (NC_044945.1:73544-74038)

ATGTATTCTAAGAAAAAGTACAT CGGT CTTATT GACAAGAACTGT GAAAAAAAAATATTAGAT GATGCTACTA CAAT AAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT TATAAC T C T TAT CT AT AAT T T CAT AT T T T G G GAG AAT T AT AT AA AAT GTATCCAAAATGAT GATAAGAT GTTTTACT GTCCTAAA GATT GGGTTGGGTATAATAATGCGTGTTATTATTTTAGTAAT AACAATAAAAATTAT ACAGAT GCAAATAATTATTGTAAAAATTCACATAATAGTACACTTGCTAATAATGATACTAAATTA TT AAAT C T T AC TAAAT TAT TAAAT C T T T C TAAAT TAT AT TACAAT GAT T CT ACAT AT T G G G T TAAAT AT T C T T TAC C TAAAAATA AAGCAGTAACATTACGTAACAGTACCTACAAATATGATAGAGTTAAATCTACAGAAACAT TTTTTATTTGTAGTAACTAA

SEQ ID No. 207 - Ken06.Bus EP153R (NC_044946.1:69244-69741)

ATGTATTCTAAGAAAAAGTACATCGGTCTTATTGACAAAAGCTGTGAAAAAAAAATA TTAGATGATCCTACTA CAAT AAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT TATAAC T C T TAT CT AT AAT T T CAT AT T T T G G GAG AAT T AT AT AA AAT GTATCCGAAATGAT GATAAGAT GTTTTACT GCCCTAAAGATTGGGTTGGATATAATAATGTGTGTTATTATTTTAGTAAC GATAATGGAAAAAATTATATGAATGCAAGTAATTTTTGTAATAAAAAAAATAGCACGCTT GCTAATAATACGACTAATTTAGT AAAT C T T AC TAAAAT AT TAAAT T T T AC CAAACAAT AT AAT CAT GAAT CACT T TAT T G G G T TAAT TAT T C T T TAAG TAAT AATA TGTCATTAGCATTACAGAATAGTAATTTTCCTTCTTCAGGAAGACGTAAATATATGGACT TATTATATATATGTAGTATATAA

SEQ ID No. 208 - L60 EP153R (NC_044941.1: 67379-67840) ATGTATTTTAAGAAAAAATACAT CGGT CTTATT GATAAGAACT GT GAAAAAAAAATATTAGAT GATT CTAGTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT GATAAC T C T TAT T T AT AAT T T CAT AT T C T G G GAT AAT T AT AT AA AATGTTACCGAAATAATGATAAAATGTTTTACTGTCCTAATGATTGGGTTGGATATAATA ATATTTGTTACTATTTTAGTAAT GGTAGTTTTTCTAAAAATTATACAGCTGCTAGTAATTTTTGTAGACAATTAAATGGTACA CTTGCTAATAATGATACTAATTT ATTAAATCTAACTAAAATATATAATAATCAATCTATGTATTGGGTTAACAATACGGTAAT ATTACGTGGTGATAATAAATATA G T C AAAAAG T T AACT AT ACAGAT T TAT TAT T TAT T T G T G GT AAAT AA

SEQ ID No. 209 - China/2018/AnhuiXCGQ EP153R (MK128995.1:72837-73313)

ATGTTTTCTAACAAAAAGTACATCGGTCTTATCAATAAGAAGGAGGGTTTGAAAAAA AAAATAGATGATTATAGTATATTAAT AAT T G GAAT AT TAAT T G GAAC TAACAT CT TAAG C C T TAT TATAAATATAAT AG GAGA GAT T AATAAAC C AATAT G T T AC CAAA AT GAT GATAAGAT AT T T TAT T G C C C TAAAGAT T G G GT T G GATATAAT AAT G T T T G T TAT TAT T T T G G CAAT GAAG AAAAAAAT T AT AATAAT G C AAGT AAT TAT T G TAAG CAAT TAAATAGT AC G C T T AC TAAT AATAAT AC TAT T T T AG TAAAT C T T AC TAAAAC ATTAAATCTTACTAAAACATATAATCACGAATCTAATTATTGGGTTAATTATTCTTTAAT TAAAAATGAGTCAGTACTATTAC G T GAT AG T G GATAT T AC AAAAAACAAAAACAT G TAAG T T TAT TAT AT AT T T GT AG TAAATAA

SEQ ID No. 210 - Malawi Lil-20/1 (1983) EP153R (AY261361.1:70776-71261)

ATGTTTT CTAACAAAAAGTACAT CGGT CTTATT GACAAGTACT GT GAAAAAAAAATATTAGAT GATT CTAGTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AC T GAT T G GAAC TAAT AT GATAAC T C T TAT T TAT AAT T T CAT AT T T T G G GAG AAT T AT AT AA CATGTAACCAAAAAGATAAGACGTTTTACTGTCCTAAAGATTGGGTTGGATATAATAATG TTTGTTATTATTTTGGCAATGAT GAAAAAAATTATAATAATGCAAGTAATTATTGTAAGCAATTAAATAGTACGCTTACTAAT AATAATACTAATTTAGTAAATCT T AC TAAAACAT TAAAT C T T AC TAAAACAT AT AAT CAC GAAT CT AAT TAT T G G G T TAAT TAT T C T T TAAT TAAAAAT GAG T CAG TACTATTACGCAATAGTGGATATTATAAAAAACAAAAACATGTAAGTTTATTATATATTT GTAGTAAATAA

SEQ ID No. 211 - Warthog EP153R (AY261366.1 :69453-69944)

ATGTTTTCTAATAAAAAGTACATTGGTCTTATCAATAAGAAGGAGGGATTGAAAAAA AAAATAGATGATTATAGTATATTAAT AAT T G GAAT AT TAAT T G GAAC TAACAT CT TAAG C C T TAT TATAAATATAAT AG GAGA GAT T AATAAAC CAATAT G T T AC CAAA ATAAT GATAAGAT AT T T TAT T G C C C TAAAGAT T G G GT T G GATATAAT AAT G T T T G T TAT TAT T T T AG TAAT GATAAT G G CAAT AAT TATACAAC T G C G GATAAT AAAT GT AAAC AAT TAAAT AATAGTACAC T G G C TAAT AAT C T T AC T GAT T TAT TAAAT C T T AC GAGTTTCTTAAATCTTACTAAATTATATCATCATCATTCTCATTATTGGGTTAATTATTC TTTAAATAATAATTATTCAGTAC CATTAATTGATAGTAAATATAATTTAAATAGGAAAAAAAGTCACTATACAGATTTACTAT TTATTTGCAGCAAATAA

SEQ ID No. 212 - Mkuzi 1979 EP153R (AY261362.1 : 74549-75013)

ATGTATTTTAAGAAAAAATACAT CGGT CTTATT GATAAGAACT GT GAAAAAAAAATATTAGAT GATT CTAGTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT GATAAC T C T TAT T TAT AAT T T CAT AT T C T G G GATAAT T AT AT AA AAT GTTACCGAAATAAT GATAAAATGTTTTACTGTCCTAAT GATT GGGTTGGATATAATAATGTTTGTTACTATTTTAGTAAT GGTAGTTTTTT TAAAAAT TAT ACAGAT G C TAAT AAT TAT T G TAAAAAAATAAATAGT AC T C T T G C TAAT AAT GAT AC TAAT T T ATTAAGTTTAACTAAAATATACAATAATCAATCTATGTATTGGGTTAACAATACAGTAGC ATTACGTGGTGATAGTGTATATG CAC AAAG AAAAGT TAAT AAT T CAGAT C TAT TAT T TAT T T GT AG TAAATAA

SEQ ID No. 213 - Warmbaths EP153R (AY261365.1 :72727-73191)

ATGTATTTTAAGAAAAAGTACAT CGGT CTTATT GATAAGAACT GT GAAAAAAAAATATTAGAT GATT GTACTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT GATAAC T C T TAT T TAT AAT T T CAT AT T T T G G GAT CAT TAT AT GA CATGTAACAAAAAAGATAAGATGTTTTACTGTCCTAAAGATTGGGTTGGATATAATAATG TTTGTTATTATTTTAGTAATGAT AGTTTTACTAAGAATTATACATATGCAAGTAATTCTTGTAAAAAAATAAATAGTACACTT GCTAATAATGATACTAATTTACT AAATCTTATTAAAGTAT AT AATAAT GTATCTGAGTATTGGGTTAATAATACAGTAACATTACGTGGT GATCAT AAAT ACAT AA ATAAGCAAAATAATTGTAATACAGAATTTTTATTTATTTGTAGTAGATAG

SEQ ID No. 214 - Tengani 62 EP153R (AY261364.1 :68134-68604)

ATGTTTTCTAACAAAAAGTATCCGAGTCTTATCGAAAAAAAAATGGATGACTTGATG ACTTTAAAGTTTTGTTACATCATTAT T GCAATCTT GATCAT GACTAATATTTTTAGCCTTGTTGTGAATATATGGGGGGGGGGTGATCGACAATCTTATGA GAATGTAT TTTATTGTCCTAAAGATTGGGTTGGATATAATAATGCATGTTATTATTTTAGTAATGATA TGAAAAATTATACAGAAGCAAGT AAT TAT T GT AAAAAT T T AT AT AATAGT AC AAT T GT TAAT AATAAT AC TAAT AT AG TAAAT C T T AC TAAAACAT TAAAT C T T AC TAAAATATATAATCATGAAAGTAATTATTGGGTTAATTATTCTTTAATTAATAATGAGTC ATTAACATTACGGGACAGTAATT TTCCTTCTTCAGGAAAACGTACATATATAGATTTATTATATATATGTAGTATATAA

SEQ ID No. 215 - Pretorisuskop/96/4 EP153R (AY261363.1:73562-74029) ATGTATTTTAAGAAAAAGTACAT CGGT CTTATT GATAAGAACT GT GAAAAAAAAATATTAGAT GATT GTACTACAATAAAAAT T T G T T ACAT AT TAAT T G GAAT AT T GAT T G GAAC TAAT AT GATAAC T C T TAT T T AT AAT T T CAT AT T T T G G GAT CAT TAT AT GA CATGTAACAAAAAAGATAAGATGTTTTACTGTCCTAAAGATTGGGTTGGATATAATAATG TTTGTTATTATTTTAATAATGAT AGTAA GAAT TATA CAACTGCTACTAATTCTTGTAAACAATTAAATAGTACACTTGCTAATAATGATACTAATT TATTAAATCT TACTAAAGTATATCACCATGATAAATTATATTGGGTTAACTATTCTTTAAATGACAATTT TTCATTATCATTACGTAATAGTA CAT AT GAAAAAAGAT CT AAAT AT T T G C CAT TAT TAT T TAT T T G CAGT AAAT AA

SEQ ID No. 216 - Kenya 1950 EP153R (AY261360.1:76255-76731)

ATGTTTTTGAACAAAAAGTATCCGAGTCTTATCGAAAAAAAAATGGATGATTTGATG ACTTTAAAGTTTTGTTACTTAATAAT CACATTTTTAATCATAACCAATATTTTTAGCCTTGCTATAAATATATGGGGGGGGGGTGA TATGATAGATCGACAAAGTTGTG AAAATATATTTTACTGTCCTAAAGATTGGGTTGGCTATAATAATGCGTGTTATTATTTTA GTAATAACAATAAAAATTATACA GATGCAAATAATTATTGTAAAAATTCACATAATAGTACACTTGCTAATAATGATACTAAA TTATTAAATCTTACTAAATTATT AAATCTTTCTAAATTATATTACAATGATTCTACATATTGGGTTAAATATTCTTTACCTAA AAATAAAGCAGTAACATTACGTA ACAGTACCTACAAATATGATAGAGTTAAATATACAGAAACATTTTTTATTTGTAGTAACT AA

In an embodiment the invention provides an attenuated ASF virus in which the expression and/or activity of the EP153R gene is disrupted. Suitably, the EP153R gene comprises the sequence of SEQ ID No. 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215 or 216. Suitably, the EP153R gene comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215 or 216. Suitably, the EP153R gene consists of the sequence of SEQ ID No. 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215 or 216.

The accession numbers of EP153R proteins from different strains of ASFV are listed below in Table 3.

Table 3 The amino acid sequences of the EP153R proteins from different ASFV strains are depicted below.

SEQ ID No. 20 - Benin 97/1 EP153R protein

MYFKKKYIGLI DKNCEKKI LDDS STIKICYI LI GI LI GTNMITLIYNFI FWDNYI KCYRNNDKMFYCPNDWVGYNNI CYYFSN

GSFSKNYTAASNFCRQLNGTLANNDTNLLNLTKIYNNQSMYWVNNTVILRGDNKYSQ KVNYTDLLFI CGK SEQ ID No. 217 - Georgia 2007/1 EP153R protein MFSNKKYIGLINKKEGLKKKIDDYSILIIGILIGTNILSLIINIIGEINKPICYQNDDKI FYCPKDWVGYNNVCYYFGNEEKN

YNNASNYCKQLNSTLTNNNTILVNLTKTLNLTKTYNHESNYWVNYSLIKNESVLLRD SGYYKKQKHVSLLYICSK*

SEQ ID No. 218 - Ken05/Tk1 EP153R protein

MYSKKKYIGLIDKNCEKKILDDATTIKICYILIGILIGTNIITLIYNFIFWENYIKC IQNDDKMFYCPKDWVGYNNACYYFSN

NNKNYTDANNYCKNSHNSTLANNDTKLLNLTKLLNLSKLYYNDSTYWVKYSLPKNKA VTLRNSTYKYDRVKSTETFFICSN*

SEQ ID No. 219 - Ken06.Bus EP153R protein

MYSKKKYIGLIDKSCEKKILDDPTTIKICYILIGILIGTNIITLIYNFIFWENYIKC IRNDDKMFYCPKDWVGYNNVCYYFSN DNGKNYMNASNFCNKKNSTLANNTTNLVNLTKILNFTKQYNHESLYWVNYSLSNNMSLAL QNSNFPSSGRRKYMDLLYICSI*

SEQ ID No. 220 - L60 EP153R protein

MYFKKKYIGLIDKNCEKKILDDSSTIKICYILIGILIGTNMITLIYNFIFWDNYIKC YRNNDKMFYCPNDWVGYNNICYYFSN

GSFSKNYTAASNFCRQLNGTLANNDTNLLNLTKIYNNQSMYWVNNTVILRGDNKYSQ KVNYTDLLFICGK*

SEQ ID No. 221 - China/2018/AnhuiXCGQ EP153R protein

MFSNKKYIGLINKKEGLKKKIDDYSILIIGILIGTNILSLIINIIGEINKPICYQND DKIFYCPKDWVGYNNVCYYFGNEEKN

YNNASNYCKQLNSTLTNNNTILVNLTKTLNLTKTYNHESNYWVNYSLIKNESVLLRD SGYYKKQKHVSLLYICSK*

SEQ ID No. 222 - Malawi Lil-20/1 (1983) EP153R protein

MFSNKKYIGLIDKYCEKKILDDSSTIKICYILIGILIGTNMITLIYNFIFWENYITC NQKDKTFYCPKDWVGYNNVCYYFGND EKNYNNASNYCKQLNSTLTNNNTNLVNLTKTLNLTKTYNHESNYWWYSLIKNESVLLRNS GYYKKQKHVSLLYICSK*

SEQ ID No. 223 - Warthog EP153R protein

MFSNKKYIGLINKKEGLKKKIDDYSILIIGILIGTNILSLIINIIGEINKPICYQNN DKIFYCPKDWVGYNNVCYYFSNDNGN

NYTTADNKCKQLNNSTLANNLTDLLNLTSFLNLTKLYHHHSHYWVNYSLNNNYSVPL IDSKYNLNRKKSHYTDLLFICSK*

SEQ ID No. 224 - Mkuzi 1979 EP153R protein

MYFKKKYIGLIDKNCEKKILDDSSTIKICYILIGILIGTNMITLIYNFIFWDNYIKC YRNNDKMFYCPNDWVGYNNVCYYFSN

GSFFKNYTDANNYCKKINSTLANNDTNLLSLTKIYNNQSMYWVNNTVALRGDSVYAQ RKWNSDLLFICSK*

SEQ ID No. 225 - Warmbaths EP153R protein

MYFKKKYIGLIDKNCEKKILDDCTTIKICYILIGILIGTNMITLIYNFIFWDHYMTC NKKDKMFYCPKDWVGYNNVCYYFSND

SFTKNYTYASNSCKKINSTLANNDTNLLNLIKVYNNVSEYWWNTVTLRGDHKYINKQ NNCNTEFLFICSR*

SEQ ID No. 226 - Tengani 62 EP153R protein

MFSNKKYPSLIEKKMDDLMTLKFCYIIIAILIMTNIFSLWNIWGGGDRQSYENVFYC PKDWVGYNNACYYFSNDMKNYTEAS NYCKNLYNSTIWNNTNIVNLTKTLNLTKIYNHESNYWVNYSLINNESLTLRDSNFPSSGK RTYIDLLYICSI*

SEQ ID No. 227 - Pretorisuskop/96/4 EP153R protein

MYFKKKYIGLIDKNCEKKILDDCTTIKICYILIGILIGTNMITLIYNFIFWDHYMTC NKKDKMFYCPKDWVGYNNVCYYFNND

SKNYTTATNSCKQLNSTLANNDTNLLNLTKVYHHDKLYWWYSLNDNFSLSLRNSTYE KRSKYLPLLFICSK*

SEQ ID No. 228 - Kenya 1950 EP153R protein

MFLNKKYPSLIEKKMDDLMTLKFCYLIITFLIITNIFSLAINIWGGGDMIDRQSCEN IFYCPKDWVGYNNACYYFSNNNKNYT DANNYCKNSHNSTLANNDTKLLNLTKLLNLSKLYYNDSTYWVKYSLPKNKAVTLRNSTYK YDRVKYTETFFICSN* Suitably, the EP153R gene encodes a protein comprising the sequence of SEQ ID No. 20, 217, 218, 219, 220, 221, 222, 223, 224, 225 226, 227 or 228. Suitably, the EP153R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 20, 217, 218, 219, 220, 221, 222, 223, 224, 225 226, 227 or 228. Suitably, the EP153R gene encodes a protein consisting of the sequence of SEQ ID No. 20, 217, 218, 219, 220, 221, 222, 223, 224, 225226, 227 or 228.

EP153R is immunogenic (i.e. evokes an immune response) (Burmakina et al. 2019 J. Gen. Virol. 100: 259-265). EP153R inhibits capsase-3 activation during ASFV infection and thereby has an inhibitory effect on apoptosis. EP153R is required for haemadsorption. EP153R also inhibits MHC-I expression in infected cells.

EP402R

The EP402R gene encodes a protein which is incorporated in the external layer of the virus and is partly similar to the mammalian T-lymphocyte surface adhesion receptor CD2. In particular, the N-terminal extracellular region of the EP402R protein consists of two immunoglobulin-like (Ig-like) domains similar to the extracellular ligand-binding region of CD2. The N-terminal extracellular domain of the EP402R protein may be referred to as the “ligand-binding domain”. The cytoplasmic domain of EP402R protein is dissimilar to CD2. The EP402R protein may be referred to as CD2v due to this similarity.

EP402R is immunogenic (i.e. evokes an immune response) (Netherton et al. 2019 Front. Immunol. 10, 1318). EP402R is required for haemadsorption and may have a role in virus entry or spread. EP402R can bind the host protein AP-1. The functions of EP402R may be mediated by its extracellular, N-terminal, Ig-like domain binding to ligands in the same manner that mammalian CD2 binds extracellular adhesion molecules.

The gene (i.e. nucleotide) sequences and positions in the genome of EP402R genes from different ASFV strains are presented below.

SEQ ID No. 229 - Georgia 2007/1 EP402R (NC_044959.1:73369-74451)

ATGATAATACTTATTTTTTTAATATTTTCTAACATAGTTTTAAGTATTGATTATTGG GTTAGTTTTAATAAAACAATAATTTT AGATAGTAATATTACTAATGATAATAATGATATAAATGGAGTATCATGGAATTTTTTTAA TAATTCTTTTAATACACTAGCTA CATGTGGAAAAGCAGGTAACTTTTGTGAATGTTCTAATTATAGTACATCAATATATAATA TAACAAATAATTGTAGCTTAACT ATTTTTCCTCATAATGATGTATTTGATACAACATATCAAGTAGTATGGAATCAAATAATT AATTATACAATAAAATTATTAAC ACCTGCTACTCCCCCAAATATCACATATAATTGTACTAATTTTTTAATAACATGTAAAAA AAATAATGGAACAAACACTAATA TATATTTAAATATAAATGATACTTTTGTTAAATATACTAATGAAAGTATACTTGAATATA ACTGGAATAATAGTAACATTAAC AATTTTACAGCTACATGTATAATTAATAATACAATTAGTACATCTAATGAAACAACACTT ATAAATTGTACTTATTTAACATT G T CAT CT AACT AT T T T TAT AC t T T T T T TAAAT TAT AT TATAT T C CAT TAAG CAT CAT AAT T G G GATAAC AATAAG TAT T CT T C TTATATCCATCATAACTTTTTTATCTTTACGAAAAAGAAAAAAACATGTTGAAGAAATAG AAAGTCCACCACCTGAATCTAAT GAAGAAGAACAATGTCAGCATGATGACACCACTTCCATACATGAACCATCTCCCAGAGAA CCATTACTTCCTAAGCCTTACAG TCGTTATCAGTATAATACACCTATTTACTACATGCGTCCCTCAACACAACCACTCAACCC ATTTCCCTTACCTAAACCGTGTC CTCCACCCAAACCATGTCCGCCACCCAAACCATGTCCTCCACCTAAACCATGTCCTTCAG CTGAATCCTATTCTCCACCCAAA CCACTACCTAGTATCCCGCTACTACCCAATATCCCGCCATTATCTACCCAAAATATTTCG CTTATTCACGTAGATAGAATTAT

TTAA

SEQ ID No. 230 - China/2018/AnhuiXCGQ EP402R (MK128995.1:73383-74465)

ATGATAATACTTATTTTTTTAATATTTTCTAACATAGTTTTAAGTATTGATTATTGG GTTAGTTTTAATAAAACAATAATTTT AGATAGTAATATTACTAATGATAATAATGATATAAATGGAGTATCATGGAATTTTTTTAA TAATTCTTTTAATACACTAGCTA CATGTGGAAAAGCAGGTAACTTTTGTGAATGTTCTAATTATAGTACATCAATATATAATA TAACAAATAATTGTAGCTTAACT ATTTTTCCTCATAATGATGTATTTGATACAACATATCAAGTAGTATGGAATCAAATAATT AATTATACAATAAAATTATTAAC ACCTGCTACTCCCCCAAATATCACATATAATTGTACTAATTTTTTAATAACATGTAAAAA AAATAATGGAACAAACACTAATA TATATTTAAATATAAATGATACTTTTGTTAAATATACTAATGAAAGTATACTTGAATATA ACTGGAATAATAGTAACATTAAC AATTTTACAGCTACATGTATAATTAATAATACAATTAGTACATCTAATGAAACAACACTT ATAAATTGTACTTATTTAACATT G T CAT CT AACT AT T T T TAT AC t T T T T T TAAAT TAT AT TATAT T C CAT TAAG CAT CAT AAT T G G GATAAC AATAAG TAT T CT T C TTATATCCATCATAACTTTTTTATCTTTACGAAAAAGAAAAAAACATGTTGAAGAAATAG AAAGTCCACCACCTGAATCTAAT GAAGAAGAACAATGTCAGCATGATGACACCACTTCCATACATGAACCATCTCCCAGAGAA CCATTACTTCCTAAGCCTTACAG TCGTTATCAGTATAATACACCTATTTACTACATGCGTCCCTCAACACAACCACTCAACCC ATTTCCCTTACCTAAACCGTGTC CTCCACCCAAACCATGTCCGCCACCCAAACCATGTCCTCCACCTAAACCATGTCCTTCAG CTGAATCCTATTCTCCACCCAAA CCACTACCTAGTATCCCGCTACTACCCAATATCCCGCCATTATCTACCCAAAATATTTCG CTTATTCACGTAGATAGAATTAT TTAA

SEQ ID No. 231 - Warthog EP402R (AY261365.1 :73281 -74450)

AT GAT AATAAT C C T TAT t T T T T T AATATAT T TAAAAC T G GT CT TAAATAAT AATAT TAT TATAT G G CAT C C TACT T TAAAT GA AACAATTTTTTTAAATGTTACAACTATAGGGAATATTAGTGGTCTATTTTGGAATACATA TTATAATAATAATCGTAGTGAAC TTACTAAGTGCGGAATATATAATAATTATTGTTCATGTGATAGTCTTAATACATCATTGT ATAATATAAATAATTGTAGTTTA G T TAT T T T T C C AAAT AATACAAAAATAT T TAAT AC AATATAT G AAT T AATATAT T CAG G TAAT CAAACT AAT T AT AC AATAAA ATTGCTAGAATCTACTACTCCTCCAAATATTACATATAATTGTACTACATCTGTAATAAC ATGTAAAAAAAATAATGGGACAA ATACTAATATATTTTTAACTATAAATAATACGATTGTTAATTCTACTAATGAAGATGTTA TTTACTACTATTGGAATAGAAGT GAATTTAATAACTTTACAGCTACATGTATGATTAATAATACAATTAATTCATCAAATATA TCAGAAATTATAGACTGTACTAA TACATTATTATTAAACAGTTATTTAGACTTTTTTCAAGTAGCCGGTTATATGTTTTATAT GATAATTTTTATTGTAACTGGAA TAACAGTAAGTATTTTTATTTCAATCATAACTTTTTTATCTTTACGAAAAAGAAAAAAAC ATGTTGAAGAAATAGAAAGTCTA CCACCTGAATCTAATGAAGAAGAATGTCAGCATGATGATGACACCACTTCCATACATGAA CCATCCCCCAGAGAACCATTACT TCCTAAACCTTACAGTCGTTATCAGTATAATACACCTATTTACTACATGCGTCCCTCAAC ACAACCACTCAACCCATTTCCCC TACCTAAACCATGCCCACCACCTAAACCGTGTCCGCCACCCAAACCATGTCCTCCACCCA AACCATGCCCGCCACCCAAACCA TGCCCGCCACCTAAACCATGTCCTCCACCTGAATCATGTTCTTCACCTGAATCATGTTCT TCACCTGAATCATATTCTCCACC CAAACCACTACCTAGTATCCCGCTGCTACCCAATATCCCGCCATTATCTACACAAAATAT TTCGCTTATTCATGTAGATAGAA T TAT TTAA

SEQ ID No. 232 - Pretorisuskop/96/4 EP402R (AY261363.1:74089-75309 )

ATGATAATGTTCATAACACTTATTTTTTTAAGTTATATTAATATAGTTTTAAGTAAT AATTATTGGGCTAGACTTAACGAAAC AAT AACTTTAAAT AGTAACATTACTAATGAT ACTAATAATGAATTAGGTATATTTTGGAATTCTTAT AATAAT ACTTATTATA ATAATACATTTAATAATATTGCTATATGTGGAAAAAAAGGTATTTTTTGTGAATGTAATA TTAATTATAATACATCTATATCT AATACATCTATATCTAATACATCAATATATAATGTAACAAATAATTGTAGTTTAACGATT TTTCTTTATGATGATAATATATT TAAAACATATCAATTAGTATATCAGAATTACAAAATTAATTATACAATAAACTTATTATT ACCTGTTACTTCTCCAAATATTA CAT AT AAT T GT AC TAAC T C T T TAAT AA CAT G T G AAAAAAAT GAT G G GAC AAACAC TAAT AT GT T T T TAT CT AT AAAT AATATA ACCATTAATCATACTAATCAAGATATTCTTACTTACTATTGGAATAATAGTGAGTTTAAT AATTTTACAGCTACATGTATGAT TAATAATACACTTAATTCAGCAAATACCACAAAAGTTATCAATTGCACTAATCCATTATT AAATTCTTACCAAAATTATTTTC TTGAAAATATTCATACATTATTTTATATCATAATTTTTATTGTGAGTGGATTAATAGCAA GTATTTTTATTTCAATTATAACT TTTTTATCTTTACGAAAAAGAAAAAAACATGTTGAAGAAATAGAAAGTCCACCACCTGAA TCTAATGAAGAAGAACAATGTCA GCATGATGACACCACTTCCATACATGAACCATCTCCCAGAGAACCATTACTTCCTAAACC TTACAGTCGTTATCAATATAATA CACCTATTTACTACATGCGTCCCTCAACACAACCACTCAACCCATTTCCCCTACCTAACC CATGTCCTCCACCTAAACCGTGT CCTCCACCCAAACCATGCCCGCCACCTAAACCATGTCCCCCACCCAAACCATGCCCGCCA CCTAAACCGTGTCCTCCACCCAA ACCATGTCCACCACCTAAACCATGTTCTTCACCTGAATCATATTCTCCACCCAAACCACT ACCTAGTATCCCGCTACTACCCA ATAT C C CAC CAT TAT CT ACAC AAAATAT T T CAC T TAT T CAT GT AGAT AG AAT TAT T T AA

SEQ ID No. 233 - L60 EP402R (NC_044941.1: 67916-69037)

ATGATAATAATAGTTATTTTTTTAATGTGTTTAAAAATAGTTTTAAACAATATTATA ATATGGAGTACTTTAAATCAAACTGT AT T T T TAAAT AAT AT T T T T AC AAT TAAT GAT ACAT AT G G T G GT CT AT T T T G GAAT ACAT AT TAT GAT AATAAT C G TAGT AAT T TTACTTATTGTGGAATAGCAGGAAATTATTGTTCATGTTGTGGTCATAACATATCATTGT ATAATACAACAAATAATTGTAGT TTAATTATTTTTCCTAACAATACAGAAATATTTAATAGAACATATGAATTAGTATATTTG GACAAAAAAATTAATTATACAGT AAAACTATTAAAATCTGTTGATTCCCCAACTATTACATATAATTGTACTAATTCTTTAAT AACATGTAAAAATAATAATGGGA CAAATGTTAATATATATTTAATTATTAATAATACAATTAGTTCATTGAATGAAACAGAAA ATATAAATTGTACTAATCCAATA TTAAAATATCAAAATTATTTATCCACATTATTTTATATCATAATTTTTATTGTGAGTGGA TTAATAATAGGTATTTTTATTTC AAT CATATCTGTATTAT CTATACGAAGAAAAAGAAAAAAACATGTTGAAGAAATAGAAAGTCCACCACCCTCT GAAT CTAATG AAGAAGATATTTCTCACGATGACACCACTTCCATACATGAACCATCTCCCAGAGAACCAT TACTTCCTAAGCCTTACAGTCGT TATCAGTATAATACACCTATTTACTACATGCGTCCCTCAACACAACCACTCAACCCATTT CCCCTACCTAAACCATGCCCGCC ACCTAAACCATGTCCTCCACCCAAGCCATGCCCGCCACCCAAACCATGTCCTCCACCTAA ACCGTGTTCTCCACCCAAACCGT GTCGTCCACCTAAACCATGTCCTCCACCTAAACCATGTCCTCCACCTAAACCATGTCCTC CACCTAAACCATGTCCTCCATCC AAACCATGTCCTTCACCTGAATCCTATTCTCCACCCAAACCACTACCTAGTATCCCGTTA CTACCCAATATCCCGCCATTATC T ACAC AAAATAT T T C G C T TAT T CAT GT AGAT AG AAT TAT T T AA

SEQ ID No. 234 - Benin 97/1 EP402R (NC_044956.1:67567-68775)

ATGATAATAATAGTTATTTTTTTAATGTGTTTAAAAATAGTTTTAAACAATATTATA ATATGGAGTACTTTAAATCAAACTGT AT T T T TAAATAAT AT T T T T AC AAT TAAT GAT ACAT AT G G T G GT CT AT T T T G GAAT ACAT AT TAT GAT AATAAT C G TAGT AAT T TTACTTATTGTGGAATAGCAGGAAATTATTGTTCATGTTGTGGTCATAACATATCATTGT ATAATACAACAAATAATTGTAGT TTAATTATTTTTCCTAACAATACAGAAATATTTAATAGAACATATGAATTAGTATATTTG GACAAAAAAATTAATTATACAGT AAAACTATTAAAATCTGTTGATTCCCCAACTATTACATATAATTGTACTAATTCTTTAAT AACATGTAAAAATAATAATGGGA C AAAT GT TAAT AT AT AT T TAAT TAT TAAC AATACAAT T G T TAAT GAT AC TAAT G GAGAT AT C C T TAAT TAT TAT T G GAAT G GT AATAATAATTTTACAGCTACATGTATGATTAATAATACAATTAGTTCATTGAATGAAACA GAAAATATAAATTGTACTAATCC AATATTAAAATATCAAAATTATTTATCCACATTATTTTATATCATAATTTTTATTGTGAG TGGATTAATAATAGGTATTTTTA TTT CAAT CATATCTGTATTAT CTATACGAAGAAAAAGAAAAAAACATGTTGAAGAAATAGAAAGTCCACCACCCTCT GAAT CT AATGAAGAAGATATTTCTCACGATGACACCACTTCCATACATGAACCATCTCCCAGAGAA CCATTACTTCCTAAGCCTTACAG TCGTTATCAGTATAATACACCTATTTACTACATGCGTCCCTCAACACAACCACTCAACCC ATTTCCCCTACCTAAACCATGCC CGCCACCTAAACCATGTCCTCCACCCAAGCCATGCCCGCCACCCAAACCATGTCCTCCAC CTAAACCGTGTTCTCCACCCAAA CCGTGTCGTCCACCTAAACCATGTCCTCCACCTAAACCATGTCCTCCACCTAAACCATGT CCTCCACCTAAACCATGTCCTCC ATCCAAACCATGTCCTTCACCTGAATCCTATTCTCCACCCAAACCACTACCTAGTATCCC GTTACTACCCAATATCCCGCCAT TAT CT ACAC AAAATAT T T C G C T TAT T CAT GT AGAT AG AAT TAT T T AA

SEQ ID No. 235 - Tengani 62 EP402R (AY261364.1 :68693-69841)

ATGATAATAAAGGTTATTTTTTTAGTTTTCTTTAAAATGGTTTTAAGTAATATTATA ATATGGAGTACTTTAAATAACACAAT ATAT T TAAATAT T AAT TAT T CAGAT AG TAAT T TAT AT AC T G GT CT AT T T T G GAAT AAAT AT TATAAT AATACT C G TAAT AAT T TTACTACTTGT GTAACATCAAATAGTAGCTATT GTACAT GTAAAGGCCATAACACAT CATT GTATAATATAACAAATAATT GT AGT T TAAT CAT T T T T C C AAAT AATACAAAAATAT T TAAT AC AACATAT GAAT TAG TATAT T TAAATAAT AAAAT TAAT TAT AC AATACAAATGGTACAACCTGTTGATCCTCCAATTATTATATATAATGATACTAATAATTC TTTAATAACATGTGAAAAAAATA AT G GAAC GAAT AC T G AAAT CT AT T TATAT T T AAAT GATACAT T TAT T AATAAT AC TAAT GAAAAT AG TAT T AAAT AC TAT T G G AATTGTAGCGAATTAAACCATAATATTACAACTACATGTATTATTAATAATACACTTAAT TCGGCAAATACCACAAAAGTTAT AAAT T G CAC TAAT CT AT T G T T AAAAT C T GAC CAAAAT TAT T T T CT TAAAAATAT T CATACAT TAT T T TATAT CAT AAT T T T TA TTGTGGGTG GAACAT TAAT AAG CAT TAT TATAT CAAT CATAAC t T T T T TAT CT T T AC GAAAAAGAAAAAAACAT G T T GAAG AA ATAGAAAGTCCACCACCCTCT GAAT CTAATGAA GAAGAA CATT GTCAGCAT GATGAT AC CACTTCCATACATGAACCATCTCC CAGAGAACCATTACTTCCTAAGCCTTACAGTCGTTATCAGTATAATACACCTATTTACTA CATGCGTCCCTCAACACAACCAC TCAACCCATTTCCCCTACCTAAACCATGTCCTCCACCTAAACCGTGTCCTCCACCCAAAC CATGTCCTCCACCTAAACCGTGT CCTCCACCTAAACCGTGTCCCCCACCTAAACCATGTCCTTCACCTGAATCCCATTCTCCA CCCAAACCACTACCTAGTATCCC GCTGCTACCCAATATTCCCCCATTATCTACACAAAATATTTCGCTTATTCATGTAGATAG AATTATTTAA

SEQ ID No. 236 - Malawi Lil-20/1 (1983) EP402R (AY261361.1:72275-72458)

AT GAT AATAAT AC T TAT t T T T T T AATAAT T C CT AACATAGT T T TAAG TAT T GAT TAT TGGGTTAGTTT T AATAAAAC AATAAT TTTAGAT AGTAAT ATTACTAATGAT AATAAT GATATAAATGGAGTATCATGGAATTTCCTTAATAATTCTCTTAATACACTAG CTACATGTGGAAAAGCAGGTAACTTTTGTGAATGCTCTAATTATAGTACATCACTATATA ATATAGCACATAATTGTAGCTTA ACTATTTTCCCTCATAATGATGTATTTGGTACACCATATCAAGTAGTATGGAATCAAATA ATTAATTATACAATAAAATTATT AACACCTGTTACTCCCCCAAATATTACATATAATTGTACTAATTTTTTAATAACATGTAA AAAAAATAATGGAACAAATACTA TTATATATTTCAATATAAATGATACTAATGTTAAATATACTAATGAAAGTATACTTGAAT ATAACTGGAATAATAGTAACTTT AACAATTTTACAGCTACATGTATAATTAATAATACAATTAATTCATCAAATGATACACAA ACTATAGACTGCATCAATACATT ATTAAGCAGTTATTTAGACTTTTTTCAAGTAGCCAGTTATATGTTTTATATGATAATTTT TATTGCAACTGGAATAATAGCAA GTATTTTTATATCAATCATAACTTTTTTATCTTTACGAAAAAGAAAAAAACATGTTGAAG AAATAGAAAGTCCATCGCCATCT GAATCTAATGAAGAAGAACAATGTCAGCATGATGACACCACTTCCATACATGAACCGTCT CCCAGAGAACCATTACTTCCTAA GCCTTACAGTCGATATCAGTATAATACACCTATTTACTATATGCGTCCCTTAACACAACC ACTCAATCCATCTCCCCTACCCA AACTGTGTCCTCCACCCAAACCGTGTCCTCCACCCAAACCGTGTCCTCCACCCAAACCGT GTCCTCCACCCAAACCGTGTCCT TCATCTGAATCATGTTCTCCACCTGAATCATATTCTCTACCCAAACCACTACCTAATATC CCGCTACTACCCAATATCCCGCC AT TAT CT ACAC AAAATAT C T C G C T TAT T CAC GT AGAT AG AAT TAT T T AA

SEQ ID No. 237 - Ken06.Bus EP402R (NC_044946.1:69827-70984)

AT GAT AATAAAG C T TAT t T T T T T AATAT CAT T T AAAAT GAT T T TAG G TAT T GAT TAT TGGGTTAGTCT T AATAAT ACAATAAT TTTAGAT AGTAACATTACTATTAATAATATTACTAATACTACTAATAATCCTACATTAAATGGTATA TTTTGGAATATTTATA ATAATAGTTATAATAATACTTTTAATTTACTTACCACATGTGGGAATACATATAATATAT GTTCTTGTTCTAATAATTATAAT ACAATATTATTTAATTATAATACCACAAATAATTGTAGTTTAATTATTTCTCCTCATGAT GAAAAAATTTTTGATACGATGTT TCAGATAATATATTTAACTAATAAAATTAATTATACAATACGATGGTTACAACCTGTTGA TCCCCCAAATATTTCATTTAATG ATAGTAATTCTTTAATAAAATGTGAAAAAAATAATGGAACAAATACTGAAATCTATTTAT ATTTAAATGATACATTTATTAAT AAT AC TAAT GAAAAT GAT C T T AAAT AT TAT T G G AAT T GT AG T GAG T T AAAC TATAAT AT TACAG C TACAT G TAT TAT TAAT AA

TACACTTAATTCGGCAAATACCACAAAAGTTATAAATTGCACTAATCTATTATTAAA ATCTGACCAAAATTATTTTCTTAAAA ACATTCATACATTATTTTATATCATAATTTTTATTGTAACTGGGATAATAATAAGTATTT TTATAGCAATCATAACTTTTTTA TCTTTACGAAAAAGAAAAAAACATGTTGAAGAAATAGAAAGTCCATCGCCTGAATCTAAT GAAGAGGAAGAACAACAACATCA TCATGACACTACTTCCATACATGAACCGTCTCCCAGAGAACCATTACTTCCTAAGCCTTA CAGTCGTTATCAGTATAATACAC CTATTTATTACATGCGTCCCTCAACACAACAACTATTTAAATCATATTCTTTACCCAAAC CATGTCCCCCACCTAAACCATGT CCTCCACCTAAACCATGTCCCCCACCCAAACCATGTCCCTCACCAGAATCATATCCCTCA CCTGAACCATATCCTCTACTACC T AATAT C C CAC TAC CAC C C AATAT C C C G C CAT TAT CT ACAC AAAATAT T T C G C T TAT T CAC GT AGAT AG AAT TAT T T AA

SEQ ID No. 238 - Ken05/Tk1 EP402R (NC_044945.1 :74107-75249)

AT GAT AATAAAAC T TAT t T T T T TAAT T T G T T T T AAAATAGT T T TAAG TAT T GATAAT AAAACAAAAT T TAAT G AAACAT TAAT TTTAGATAATATTGATTATTGGGTAAAGTTTAATGATACAATAACTTTAGATAGTAATAT TACTAGTGAAATAGAAGCTGTAT CTTGGAATTTTTATAATAATACTTTTAATCTACTTGCTACATGTGGAAAAGCAAGTAACT TTTGTAGTTGTTCTAATTATAGT ACATCATTTTTTAATATTACAAATAATTGTAGTTTAACTATTTTTTTAATAGACGAGACG TTATTTAATACAACATATCAAAT AGTATATTCAACTAATATAATTAATTATAAAATAAACTTATTAATACCTGTTACTCCTCC AATTATTTCATATAATTGTACTA ATTGTTCTATAAATTGTAAAAAAAGTAATGGTACAAATACTAATATTTTTTTATCTATAA ATGATACAATTGTTACATATACT AATGAAAGTATTCTTAATTATGATTATAACTGTAGTATTTTAAATAATAATTTTACAGTT ACATGTATAATTAATAATACAAT TAGTACATCAAATACTACAGAATTTATAAACTGCACTAATATATTATTAAGTAGTTATCT AGATTTTTTTCAAGTAACTAGTT ATATATTTTATATGATAATTTTTATTGTAACTGGAATAACAGTAAGTATTCTTATATCCA TCATAACTTTCTTATTTATACGA AAAAGAAAACATGTTGAAGAAATAGAAAGTCCACCGCCTGAATCTAATGAAGAAGAACAA CAATGTCATCATGACACCACTTC TATACATGAACCGTCTCCCAGAGAACCATTACTTCCTAAGCCTTACAGTCGTTATCAATA TAATACACCTATTTACTACATGC GTCCCTCAACACAACAACTATTTAAATCATATTCTTTACCCAAACCATGTCCCCCACCTA AACCATGTCCTCCACCCAAACCA TGTCCCCCACCCAAACCATGTCCTCCACCCAAACCATGTCCTCCATCCAAACCATATCCT TCACTACCTAGTATCCCGCTACC ACCCGATATCCCGCCATTATCTACACAAAATATTTCGCTTATTCACGTAGATAGAATTAT TTAA

SEQ ID No. 239 - Kenya 1950 EP402R (AY261360.1:76800-77978)

AT GAT AATAAAAC T TAT t T T T T TAAT T T G T T T T AAAATAGT T T TAAG TAT T GATAAT AAAACAAAAT T TAAT G AAACAT TAAT TTTAGATAATATTGATTATTGGGTAAAGTTTAATGATACAATAACTTTAGATAGTAATAT TACTAGTGAAATAGAAGCTGTAT CTTGGAATTTTTATAATAATACTTTTAATCTACTTGCTATATGTGGAAAAGCAAGTAACT TTTGTAGTTGTTCTAATTATAGT ACATCATTTTTTAATATTACAAATAATTGTAGTTTAACTATTTTTTTAATAGACGAGACG TTATTTAATACAACATATCAAAT AGTATATTCAACTAATATAATTAATTATAAAATAAACTTATTAATACCTGTTACTCCTCC AATTATTTCATATAATTGTGCTA ATTGTTCTATAAATTGTAAAAAAAGTAATGGTACAAATACTAATATTTTTTTATCTATAA ATGATACAATTGTTACATATACT AATGAAAGTATTCTTAATTATGATTATAACTGTAGTATTTTAAATAATAATTTTACAGTT ACATGTATAATTAATAATACAAT TAGCACATCAAATACTACAGAATTTATAAACTGCACTAATATATTATTAAGTAGTTATCT AGATTTTTTTCAAGTAACTAGTT ATATATTTTATATGATAATTTTTATTGTAACTGGAATAACAGTAAGTATTCTTATATCCA TCATAACTTTCTTATTTATACGA AAAAGAAAACATGTTGAAGAAATAGAAAGTCCACCGCCTGAATCTAATGAAGAAGAACAA CAATGTCATCATGACACCACTTC TATACATGAACCGTCTCCCAGAGAACCATTACTTCCTAAGCCTTACAGTCGTTATCAATA TAATACACCTATTTACTACATGC GTCCCTCAACACAACAACTATTTAAATCATATTCTTTACCCAAACCATGTCCCCCACCTA AACCATGTCCTCCACCTAAACCA TGTCCCCCACCTAAACCATGTCCTCCATCCAAACCATGTCCTCCACCTGAACCATATTCT CCACCCAAACCATGTCCTCCACC CAAACCATATCCTTCACTACCTAGTATCCCGCTACCACCCGATATCCCGCCATTATCTAC ACAAAATATTTCGCTTATTCACG T AGAT AG AAT TAT T T AA

SEQ ID No. 240 - Warthog EP402R (AY261366.1 :70011-71156) AT GATAAT T AAAC T TAT t T T T T TAAT T T G T T T C AAAATAGT T T TAAG TAT TAAT TAT TGGGTTAG GT AT AAT GAT ACAG TAAC

T T T AAAT AG TAAT AT CAAT AG C GAGACAG AAG G TATAT T T T G G AAT T T T TATAAT AATACT T T TAAT AC TAT T G C CACAT G T G GAAAAAAAAATAATGTTTGTGAATGTTCTAATTATGATAAATCCTTATATAATATAACAA ATAATTGTAGTTTAACAATTTTT C CAAATAAT AC AAAAAT AT T T AATACAACAT AT CAAT TAGTATAT T CAC GCAATAGAAT TAAT TACACAATAAAT TT GT TATT ACCTGTTACATCTCCAATTATTACATATAATTGTACTCAATCTTTAATAACATGTGAAAA AACTAATGGAACAAATATTCATT TATTCTTAAATTTAAATGATACAATTAATGAATATACTAACAAAAGTTTTCTCAATTACT ATTGGAATAGTAGTGAGTTAAAT AAT ATTTTT CTTGCTACAT GTATTATTAATAAT ACACTTAATTCAGCAAAT ACTA CAAAAGTTATCAATTGCACTAACCCATT AT T AAAAT C T T AC CAAAAT TAT T T T CT T G AAAATAT T CATACAT TAT T T TATAT GATAAT T T T TAT T GT AT CT G G AATAAC AA TAAGTATTTTTATATCAATCATAACTTTTTTATCTTTACGAAAAAGAAAAAAACATGTTG AAGAAATAGAGAGTCCACCACCT GAATCTAATGAAGAAGAACAATGTCAGCATGATGACACCACTTCTATACATGAACCATCT CCCAGAGAACCATTACTTCCTAA ACCTTACAGTCGTTATCAGTATAATACACCTATTTACTACATGCGTCCCTCAACACAACC ACTCAACCCATTTCCCCTACCTA AACCATGCCCGCCACCTAAACCGTGTCCTCCACCCAAACCATGCCCGCCACCCAAACCAT GCCCGCCACCTAAACCATGTCCT CCACCCAAACCATGCCCGCCACCTAAACCATGTCCTCCACCTGAATCATATTCTCCACCC AAACCACTACCTAGTATCCCGCT G CT AC C C AATAT C C C G C CAT TAT CT ACAC AAAATAT T T C G C T TAT T CAT GT AGAT AG AAT TAT T T AA SEQ ID No. 241 - Mkuzi 1979 EP402R (AY261362.1 : 75106-76287)

ATGATGATAATTATACTTATTTTTTTAATATGTTTAAAAATAGTTTTAAATAATAAT ATTATAATATGGCATACTTTAAATGA AACAATTTTTTTAAATAATAGTATTACATTTACGGATAATCTTGGTGGTCTATTTTGGAA TACATATTATAATAATAATCATA GTATCCTTACTAGTTGCGGAATATATCAAAATTATTGTTCATGCACTAATAATTATAGCA CATCATTATATAATATAACAAAT AATTGTGGTTTAATAATTTTTCCAAATAATACAAAAAGATTTAACACAACGTATGAATTA GTATATTTAAATAATAGTACTAA ATATACAATAAACTTATTAGTACCTGTTACTCCTCCAAATATTACATATAATTGTACTAC ATCTGTAATAACATGTAAAAAAA ATGATGGCACAAATACTAATATGTTTTTAAAAATAAATGATACAATCGTTAAAAGTAGTA ATGAAAGTGTTTTTGAATACTCT TGGAATAGTACTGATTTAAATAACAATTTTACGGCTATATGTATAATTAATAATACAATT AATTCATCAAATGACACACAAAC TATAGAATGTCCCAATATATTATTAAACAATTATTTAGACTTTTTTCAAGTAACTAGTTA CATGTTTTATATGATAATTTTTA TTGTAACTGGAATAATAGCAAGTATTTTTATTTCAATCATAACTTTTTTATCTTTACGAA AAAGAAAAAAACATGTTGAAGAA ATAGAAAGTCCACCACCCTCTGAATCTAATGAAGAAGATATTTCTCACGATGACACCACT TCCATACATGAACCATCTCCCAG AGAACCATTACTTCCTAAGCCTTACAGTCGTTATCAGTATAATACACCTATTTACTACAT GCGTCCCTCAACACAACCACTCA ACCCATTTCCCCTACCTAAACCATGCCCGCCACCTAAACCATGCCCGCCACCCAAACCAT GTCCTCCACCTAAACCGTGTCCT CCACCCAAACCGTGTCGTCCACCTAAACCATGTCCTCCACCTAAACCATGCCCACCACCT AAACCATGTCCTTCACCTGAAAC ATATTCTCCACCCAAACCACTACCTAATATCCCGCTGCTACCCAATATCCCGCCATTATC TACACAAAATATTTCGCTTATTC ACGTAGATAGGATTATTTAA

In an embodiment the invention provides an attenuated ASF virus in which the expression and/or activity of the EP402R gene is disrupted. Suitably, the EP402R gene comprises the sequence of SEQ ID No. 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240 or 241. Suitably, the EP402R gene comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240 or 241. Suitably, the EP402R gene consists of the sequence of SEQ ID No. 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240 or 241.

The amino acid sequence of the EP402R protein from the Benin 97/1 strain is depicted below as SEQ ID No. 21.

SEQ ID No. 21 - Benin 97/1 EP402R protein

MilIVIFLMCLKIVLNNIIIWSTLNQTVFLNNIFTINDTYGGLFWNTYYDNNRSNFT YCGIAGNYCSCCGHNISLYNTTNNCS LIIFPNNTEIFNRTYELVYLDKKINYTVKLLKSVDSPTITYNCTNSLITCKNNNGTNVNI YLIINNTIVNDTNGDILNYYWNG NNNFTATCMINNTISSLNETENINCTNPILKYQNYLSTLFYIIIFIVSGLIIGIFISIIS VLSIRRKRKKHVEEIESPPPSES NEEDISHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPPPKPCSPPK PCRPPKPCPPPKPCPPPKPCPPPKPCPPSKPCPSPESYSPPKPLPSIPLLPNIPPLSTQN ISLIHVDRII

Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 21. Suitably, the EP402R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 21. Suitably, the EP402R gene encodes a protein consisting of the sequence of SEQ ID No. 21.

The ligand-binding domain of Benin 97/1 EP402R is formed by amino acids 1-198 of SEQ ID No. 21 which are presented below as SEQ ID No. 34.

SEQ ID No. 34 - Benin 97/1 EP402R protein ligand-binding domain

MIIIVIFLMCLKIVLNNIIIWSTLNQTVFLNNIFTINDTYGGLFWNTYYDNNRSNFT YCGIAGNYCSCCGHNISLYNTTNNCS LIIFPNNTEIFNRTYELVYLDKKINYTVKLLKSVDSPTITYNCTNSLITCKNNNGTNVNI YLIINNTIVNDTNGDILNYYWNG NNNFTATCMINNTISSLNETENINCTNPILKY Suitably, the ligand-binding domain of the EP402R protein comprises the sequence of SEQ ID No. 34. Suitably, the ligand-binding domain of the EP402R protein comprises a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 34. Suitably, the ligand-binding domain of the EP402R protein consists of the sequence of SEQ ID No. 34.

The orthologous EP402R genes from other ASFV isolates are depicted below as SEQ ID Nos 22 to 30:

SEQ ID No. 22 - Mkuzi EP402R protein

MIIILIFLICLKIVLNNNIIIWHTLNETIFLNNSITFTDNLGGLFWNTYYNNNHSIL TSCGIYQNYCSCTNNYSTSLYNITNN CGLIIFPNNTKRFNTTYELVYLNNSTKYTINLLVPVTPPNITYNCTTSVITCKKNDGTNT NMFLKINDTIVKSSNESVFEYSW NSTDLNNNFTAICIINNTINSSNDTQTIECPNILLNNYLDFFQVTSYMFYMIIFIVTGII ASIFISIITFLSLRKRKKHVEEI ESPPPSESNEEDISHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLP KPCPPPKPCPPPKPCPPPKPCPP PKPCRPPKPCPP PKPCPPPKPCPSPETYSPPKPLPNIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 23 - Warmbaths EP402R protein

MIIILIFLIYLKLVLNNNIIIWHPTLNETIFLNVTTIGNISGLFWNTYYNNNRSELT KCGIYNNYCSCDSLNTSLYNINNCSL VIFPNNTKIFNTIYELIYSGNQTNYTIKLLESTTPPNITYNCTTSVITCKKNNGTNTNIF LTINNTIVNSTNEDVIYYYWNRS EFNNFTATCMINNTINSSNISEIIDCTNTLLLNSYLDFFQVAGYMFYMIIFIVTGITVSI FISIITFLSLRKRKKHVEEIESL PPESNEEECQHDDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPC PPPKPCPPPKPCPPPKPCPPPKP CPPPKPCPPPESCSSPESCSSPESYSPPKPLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 24 - Georgia 2007/1 EP402R protein

MIILIFLIFSNIVLSIDYWVSFNKTIILDSNITNDNNDINGVSWNFFNNSFNTLATC GKAGNFCECSNYSTSIYNITNNCSLT IFPHNDVFDTTYQWWNQIINYTIKLLTPATPPNITYNCTNFLITCKKNNGTNTNIYLNIN DTFVKYTNESILEYNWNNSNIN NFTATCIINNTISTSNETTLINCTYLTLSSNYFYTFFKLYYIPLSIIIGITISILLISII TFLSLRKRKKHVEEIESPPPESN EEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPSAESYSPPK PLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 25 - Malawi Lil-20/1 (1983) EP402R protein

MilILIFLIIPNIVLSIDYWVSFNKTIILDSNITNDNNDINGVSWNFLNNSLNTLAT CGKAGNFCECSNYSTSLYNIAHNCSL TIFPHNDVFGTPYQVWNQIINYTIKLLTPVTPPNITYNCTNFLITCKKNNGTNTIIYFNI NDTNVKYTNESILEYNWNNSNF NNFTATCIINNTINSSNDTQTIDCINTLLSSYLDFFQVASYMFYMIIFIATGIIASIFIS IITFLSLRKRKKHVEEIESPSPS ESNEEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPLTQPLNPSPLPKLCPP PKPCPPPKPCPPPKPCPPPKPCP SSESCSPPESYSLPKPLPNIPLLPNIPPLSTQN ISLIHVDRII

SEQ ID No. 26 - Kenya 1950 EP402R protein

MIIKLIFLICFKIVLSIDNKTKFNETLILDNIDYWVKFNDTITLDSNITSEIEAVSW NFYNNTFNLLAICGKASNFCSCSNYS TSFFNITNNCSLTIFLIDETLFNTTYQIVYSTNIINYKINLLIPVTPPIISYNCANCSIN CKKSNGTNTNIFLSINDTIVTYT NESILNYDYNCSILNNNFTVTCIINNTISTSNTTEFINCTNILLSSYLDFFQVTSYIFYM IIFIVTGITVSILISIITFLFIR KRKHVEEIESPPPESNEEEQQCHHDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQ QLFKSYSLPKPCPPPKPCPPPKP CPPPKPCPPSKPCPPPEPYSPPKPCPPPKPYPSLPSIPLPPDIPPLSTQNISLIHVDRII

SEQ ID No. 27 - N10 EP402R protein

MIIKLIFLISFKMILGIDYWVSLNNTIILDSNITINNITNTTNNPTLNGIFWNIYNN SYNNTFNLLTTCGNTYNICSCSNNYN TILFNYNTTNNCSLIISPHDEKIFDTMFQIIYLTNKINYTIRWLQPVDPPNISFNDSNSL IKCEKNNGTNTEIYLYLNDTFIN NTNENDLKYYWNCSELNYNITATCIINNTLNSANTTKVINCTNLLLKSDQNYFLKNIHTL FYI11FIVTGI11SIFIAIITFL SLRKRKKHVEEIESPSPESNEEEEQQHHHDTTSIHEPSPREPLLPKPYSRYQYNTPIYYM RPSTQQLFKSYSLPKPCPPPKPC PPPKPCPPPKPCPSPESYPSPEPYPLLPNIPLPPNIPPLSTQNISLIHVDRII SEQ ID No. 28 - Tengani 62 EP402R protein

MIIKVIFLVFFKMVLSNIIIWSTLNNTIYLNINYSDSNLYTGLFWNKYYNNTRNNFT TCVTSNSSYCTCKGHNTSLYNITNNC SLIIFPNNTKIFNTTYELVYLNNKINYTIQMVQPVDPPIIIYNDTNNSLITCEKNNGTNT EIYLYLNDTFINNTNENSIKYYW NCSELNHNITTTCIINNTLNSANTTKVINCTNLLLKSDQNYFLKNIHTLFYIIIFIVGGT LISIIISIITFLSLRKRKKHVEE IESPPPSESNEEEHCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFP LPKPCPPPKPCPPPKPCPPPKPC PPPKPCPPPKPCPSPESHSPPKPLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 29 - Pr4 EP402R protein

MFITLIFLSYINIVLSNNYWARLNETITLNSNITNDTNNELGIFWNSYNNTYYNNTF NNIAICGKKGIFCECNINYNTSISNT SISNTSIYNVTNNCSLTIFLYDDNIFKTYQLVYQNYKINYTINLLLPVTSPNITYNCTNS LITCEKNDGTNTNMFLSINNITI NHTNQDILTYYWNNSEFNNFTATCMINNTLNSANTTKVINCTNPLLNSYQNYFLENIHTL FYIIIFIVSGLIASIFISIITFL SLRKRKKHVEEIESPPPESNEEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMR PSTQPLNPFPLPNPCPPPKPCPP PKPCPPPKPCPPPKPCPPPKPCPPPKPCPPPKPCSSPESYSPPKPLPSIPLLPNIPPLST QNISLIHVDRII

SEQ ID No. 30 - Warthog EP402R protein

MIIKLIFLICFKIVLSINYWVRYNDTVTLNSNINSETEGIFWNFYNNTFNTIATCGK KNNVCECSNYDKSLYNITNNCSLTIF PNNTKIFNTTYQLVYSRNRINYTINLLLPVTSPIITYNCTQSLITCEKTNGTNIHLFLNL NDTINEYTNKSFLNYYWNSSELN NIFLATCIINNTLNSANTTKVINCTNPLLKSYQNYFLENIHTLFYMIIFIVSGITISIFI SIITFLSLRKRKKHVEEIESPPP ESNEEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPP PKPCPPPKPCPPPKPCPPPKPCP PPKPCPP PKPCPPPESYSPPKPLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 242 - China/2018/AnhuiXCGQ EP402R protein

MIILIFLIFSNIVLSIDYWVSFNKTIILDSNITNDNNDINGVSWNFFNNSFNTLATC GKAGNFCECSNYSTSIYNITNNCSLT IFPHNDVFDTTYQWWNQIINYTIKLLTPATPPNITYNCTNFLITCKKNNGTNTNIYLNIN DTFVKYTNESILEYNWNNSNIN NFTATCIINNTISTSNETTLINCTYLTLSSNYFYTFFKLYYIPLSIIIGITISILLISII TFLSLRKRKKHVEEIESPPPESN EEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPSAESYSPPK PLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 243 - Pretorisuskop/96/4 EP402R protein

MIMFITLIFLSYINIVLSNNYWARLNETITLNSNITNDTNNELGIFWNSYNNTYYNN TFNNIAICGKKGIFCECNINYNTSIS NTSISNTSIYNVTNNCSLTIFLYDDNIFKTYQLVYQNYKINYTINLLLPVTSPNITYNCT NSLITCEKNDGTNTNMFLSINNI TINHTNQDILTYYWNNSEFNNFTATCMINNTLNSANTTKVINCTNPLLNSYQNYFLENIH TLFYIIIFIVSGLIASIFISIIT FLSLRKRKKHVEEIESPPPESNEEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYY MRPSTQPLNPFPLPNPCPPPKPC PPPKPCPPPKPCPPPKPCPPPKPCPPPKPCPPPKPCSSPESYSPPKPLPSIPLLPNIPPL STQNISLIHVDRII

SEQ ID No. 244 - L60 EP402R protein

MilIVIFLMCLKIVLNNIIIWSTLNQTVFLNNIFTINDTYGGLFWNTYYDNNRSNFT YCGIAGNYCSCCGHNISLYNTTNNCS LIIFPNNTEIFNRTYELVYLDKKINYTVKLLKSVDSPTITYNCTNSLITCKNNNGTNVNI YLIINNTISSLNETENINCTNPI LKYQNYLSTLFYIIIFIVSGLIIGIFISIISVLSIRRKRKKHVEEIESPPPSESNEEDIS HDDTTSIHEPSPREPLLPKPYSR YQYNTPIYYMRPSTQPLNPFPLPKPCPPPKPCPPPKPCPPPKPCPPPKPCSPPKPCRPPK PCPPPKPCPPPKPCPPPKPCPPS KPCPSPESYSPPKPLPSIPLLPNIPPLSTQNISLIHVDRII

SEQ ID No. 245 - Ken06.Bus EP402R protein

MIIKLIFLISFKMILGIDYWVSLNNTIILDSNITINNITNTTNNPTLNGIFWNIYNN SYNNTFNLLTTCGNTYNICSCSNNYN TILFNYNTTNNCSLIISPHDEKIFDTMFQIIYLTNKINYTIRWLQPVDPPNISFNDSNSL IKCEKNNGTNTEIYLYLNDTFIN NTNENDLKYYWNCSELNYNITATCIINNTLNSANTTKVINCTNLLLKSDQNYFLKNIHTL FYI11FIVTGI11SIFIAIITFL SLRKRKKHVEEIESPSPESNEEEEQQHHHDTTSIHEPSPREPLLPKPYSRYQYNTPIYYM RPSTQQLFKSYSLPKPCPPPKPC PPPKPCPPPKPCPSPESYPSPEPYPLLPNIPLPPNIPPLSTQNISLIHVDRII

SEQ ID No. 246 - Ken05/Tk1 EP402R protein

MIIKLIFLICFKIVLSIDNKTKFNETLILDNIDYWVKFNDTITLDSNITSEIEAVSW NFYNNTFNLLATCGKASNFCSCSNYS TSFFNITNNCSLTIFLIDETLFNTTYQIVYSTNIINYKINLLIPVTPPIISYNCTNCSIN CKKSNGTNTNIFLSINDTIVTYT NESILNYDYNCSILNNNFTVTCIINNTISTSNTTEFINCTNILLSSYLDFFQVTSYIFYM IIFIVTGITVSILISIITFLFIR KRKHVEEIESPPPESNEEEQQCHHDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQ QLFKSYSLPKPCPPPKPCPPPKP CPPPKPCPPPKPCPPSKPYPSLPSIPLPPDIPPLSTQNISLIHVDRII

The ligand-binding domain of the EP402R protein from other strains can be readily identified by alignment with the sequence of Benin 97/1 EP402R protein, such as shown in Figure 3.

In an embodiment the invention provides an attenuated ASF virus in which the expression and/or activity of the EP402R gene is disrupted. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 22. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 23. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 24. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 25. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 26. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 27. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 28. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 29. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 30. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 242. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 243. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 244. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 245. Suitably, the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 246.

Haemadsorption

Haemadsorption is the phenomenon whereby cells infected with ASFV adsorb erythrocytes (red blood cells) on their surface. The degree of haemadsorption induced by an ASFV may be measured using a haemadsorption assay such as described herein. For example, cells may be transfected with a protein or infected with an ASFV, then red blood cells added and the degree of haemadsorption detected by imaging. In this way, different proteins and viruses can be tested for their effect on haemadsorption.

EP402R and EP153R are involved in mediating haemadsorption of ASFV-infected cells.

In an embodiment the invention provides an attenuated ASF virus which has reduced haemadsorption.

In an embodiment of the attenuated ASF virus of the invention, the ability of the EP153R gene and/or the EP402R gene to mediate haemadsorption may be disrupted. In an embodiment, the ability of the EP153R gene to mediate haemadsorption may be disrupted. In an embodiment, the ability of the EP402R gene to mediate haemadsorption may be disrupted.

Reducing haemadsorption or disrupting the ability to mediate haemadsorption means that cells infected with the attenuated ASFV of the invention or transfected with a mutant, non- functional EP153R or EP402R protein adsorb fewer red blood cells to their surface than cells infected with a wild-type ASFV or transfected with a wild-type EP153R or EP402R protein. The number of red blood cells adsorbed to the surface of the infected/transfected cells may be decreased by at least 50, 60, 70, 80 or 90%. In an embodiment, haemadsorption is abolished i.e. no red blood cells adsorb to the surface of cells infected with the attenuated ASFV of the invention or transfected with a mutant, non-functional EP153R or EP402R protein.

Gene expression

The attenuated African Swine Fever (ASF) virus of one embodiment of the present invention has disrupted expression and/or activity of the genes DP148R, EP153R and EP402R. These genes may be referred to herein as the “disrupted genes”.

In an embodiment the invention provides an attenuated ASFV in which expression of the genes DP148R, EP153R and EP402R is disrupted.

The term “expression” with respect to a gene refers to the ability of the ASF virus to produce the product of the gene, such as RNA and/or protein. Disruption of expression of a gene means that production of the gene product is decreased. Expression of the gene may be decreased by at least 50%, at least 60%, at least 70%, at least 80%, at least 90% and/or at least 95%. Expression of the gene may be decreased to the extent that production of the gene product, such as RNA and/or protein, is entirely abolished (i.e. the gene product is not produced at all). Disruption of gene expression decreases expression of the gene relative to the expression of the gene when it is not disrupted. For example, a mutated gene may have decreased expression in comparison to a wild-type version of the gene.

A gene the expression of which is disrupted may not be fully transcribed and translated. Transcription of the gene may be decreased by at least 50%, at least 60%, at least 70%, at least 80%, at least 90% and/or at least 95%. Transcription of the gene may be abolished (i.e. the gene may not be transcribed). Translation of the gene may be decreased by at least 50%, at least 60%, at least 70%, at least 80%, at least 90% and/or at least 95%. Translation of the gene may be abolished. The gene may be transcribed but not translated. The gene may be transcribed and translated but the protein too rapidly degraded to carry out its function. The gene may be transcribed and translated but the protein may be non-functional.

Gene expression may be measured by techniques known in the art. For example, the amount of mRNA transcribed from a gene may be quantified, such as by using quantitative polymerase chain reaction (qPCR). Alternatively or additionally, the amount of protein may be quantified, such as by using Western blotting or mass spectrometry.

Gene activity

In an embodiment the invention provides an attenuated ASFV in which activity of the genes DP148R, EP153R and EP402R is disrupted.

The term “activity” with respect to a gene refers to the ability of the gene to carry out its functions. Different genes have different activities i.e. different functions they fulfil. A given gene may have multiple activities; disruption of gene activity means disruption of one or more of those activities. One or more activity of the gene may be disrupted whilst one or more other activities are not disrupted. Disruption of gene activity decreases the activity of the gene relative to the activity of the gene when it is not disrupted. For example, a mutated gene may have decreased activity in comparison to a wild-type version of the gene. Gene activity may be decreased to the extent that gene activity is entirely abolished.

The attenuated ASFV according to the present invention may comprise a non-functional version of the disrupted genes.

Disruption of expression of a gene may also disrupt activity of that gene as the decreased amount of gene product means the gene cannot as effectively carry out one or more of its activities.

Gene expression and/or activity are disrupted by mutating the ASFV genome i.e. by mutating the ASFV genome. A “mutation” means a change in the nucleotide sequence of the ASFV genome relative to a known ASFV genotype. Mutations include changing one or more nucleotides to different nucleotides (i.e. substitution), adding nucleotides, deleting nucleotides and/or a combination of these.

In an embodiment the attenuated ASF virus of the invention comprises mutations that disrupt the expression and/or activity of the genes DP148R, EP153R and EP402R.

Gene expression and/or activity may be disrupted by disrupting transcription of the gene into mRNA i.e. by decreasing gene transcription, such as completely abolishing gene transcription. Gene expression and/or activity may be disrupted by disrupting translation of mRNA into protein. In an embodiment the attenuated ASF virus comprises mutations that decrease transcription and/or translation of the genes. In an embodiment the attenuated ASF virus comprises mutations that cause the genes to not be transcribed and/or translated (i.e. complete abolition of transcription and/or translation).

Gene expression and/or activity may be disrupted by mutating a non-coding sequence associated with the gene, such as a promoter. In an embodiment the attenuated ASF virus comprises mutations in promoters of one or more of the disrupted genes.

Gene expression and/or activity may be disrupted by mutating a coding sequence of one or more of the disrupted genes.

Activity of DP148R

In an embodiment of the attenuated ASFV of the invention, DP148R activity may be disrupted.

In an embodiment the activity of DP148R that may be disrupted is its ability to inhibit type I interferon. In other words, the ability of DP148R to inhibit type I interferon may be decreased. The ability of DP148R to inhibit type I interferon may be decreased by at least 50, 60, 70, 80 or 90%. The ability of DP148R to inhibit type I interferon may be completely abolished.

In an embodiment the activity of DP148R that may be disrupted is its ability to inhibit NF-kB activity. In an embodiment, the invention provides an attenuated ASF virus in which the ability of the DP148R gene to inhibit NFKB activity is disrupted. NFKB activity may be measured using a luciferase reporter assay as described herein.

Disruption of the ability to inhibit NFKB activity means that the ability to inhibit NFKB activity is decreased. In other words, the DP148R gene of the attenuated ASF virus of the invention inhibits NFKB activity less than would a wild-type version of the DP148R gene. The ability to inhibit NFKB activity may be decreased by at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%. In an embodiment the ability of the DP148R gene to inhibit NFKB activity may be abolished i.e. the DP148R gene does not inhibit NFKB activity. In that case, the activity of NFKB would be as high as if no DP148R gene were present. A decrease in inhibition means NFKB activity is higher than it would be with the wild-type version of the DP148R gene. NFKB activity may be twice as high as it would be with the wild type version of the DP148R gene, such as three times as high, four times as high, five times as high or six times as high. NFKB is a transcription factor that activates transcription of various genes, controlling expression of interferon and pro-inflammatory cytokines as part of the host’s innate immune system response to viral infection. Inhibition of NFKB activity means decreasing activation of expression of its target genes. The degree of activation of NFKB target gene expression may be measured using a luciferase reporter assay in which an NFKB-activatable promoter is linked to a luciferase reporter.

In an embodiment the activity of DP148R that may be disrupted is its ability to inhibit translocation of the p65 sub-unit of NFKB to the nucleus. In an embodiment, the invention provides an attenuated ASF virus in which the ability of the DP148R gene to inhibit translocation of the p65 sub-unit of NFKB to the nucleus is disrupted. Translocation of p65 to the nucleus can be measured by immunofluorescence of cells stimulated with NF-kB activators as described herein. The relative and absolute amounts of p65 that translocate to the nucleus may quantified by using differential cell lysis conditions known in the art to either break open the nucleus or leave it intact then quantify the amount of p65 using techniques known in the art such as Western blotting or mass spectrometry. For example, cells expressing a wild type or mutant DP148R protein may be stimulated to activate NF-kB then differentially lysed to produce whole cell lysate, a cytoplasmic fraction and a nuclear fraction. The amount of p65 in each fraction may then be quantified for the different conditions (wild type or mutant DP148R protein).

Disruption of the ability to inhibit translocation of p65 to the nucleus means that the ability to inhibit translocation of p65 to the nucleus is decreased. In other words, the version of the DP148R gene in the attenuated ASF virus of the invention inhibits translocation of p65 to the nucleus less than would a wild-type version of the DP148R gene. The ability to inhibit translocation of p65 to the nucleus may be decreased by at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%. In an embodiment the ability of the DP148R gene to inhibit translocation of p65 to the nucleus may be abolished i.e. the DP148R gene does not inhibit translocation of p65 to the nucleus. In that case, as much p65 would translate to the nucleus as if no DP148R gene were present. A decrease in inhibition means the proportion of p65 that translocates to the nucleus is higher than it would be with the wild-type version of the DP148R gene. The proportion of p65 that translocates to the nucleus may be twice as high as it would be with the wild type version of the DP148R gene, such as three times as high, four times as high, five times as high or six times as high.

Activity of EP153R

In an embodiment of the attenuated ASFV of the invention, EP153R activity may be disrupted. In an embodiment the activity of EP153R that may be disrupted is its ability to mediate haemadsorption. In other words, the ability of EP153R to mediate haemadsorption may be decreased. The ability of EP153R to mediate haemadsorption may be decreased by at least 50, 60, 70, 80 or 90%. The ability of EP153R to mediate haemadsorption may be completely abolished.

In an embodiment the activity of EP153R that may be disrupted is its ability to inhibit caspase-3. In other words, the ability of EP153R to inhibit caspase-3 may be decreased. The ability of EP153R to inhibit caspase-3 may be decreased by at least 50, 60, 70, 80 or 90%. The ability of EP153R to inhibit caspase-3 may be completely abolished. Caspase-3 activity may be measured by assays known in the art, such as described by Hurtado et al. (Hurtado et al. 2004 Virology 326: 160-170). For example, the cleaved active caspase-3 fragment of 17 kDa and the inactive procaspase-3 protein of 34 kDa may be quantified using Western blot or mass spectrometry and compared to ascertain the degree of activation of caspase-3. Alternatively or additionally, the ability of caspase-3 in cell extract to cleave a fluorescent substrate may be measured using high performance liquid chromatography.

In an embodiment the activity of EP153R that may be disrupted is its ability to inhibit MHC-I expression. In other words, the ability of EP153R to inhibit MHC-I expression may be decreased. The ability of EP153R to inhibit MHC-I expression may be decreased by at least 50, 60, 70, 80 or 90%. The ability of EP153R to inhibit MHC-I expression may be completely abolished. MHC-I expression may be measured by assays known in the art, such as described by Hurtado et al. (Hurtado et al. 2011 Arch. Virol. 156(2): 219-234). For example, cell surface expression of MHC-I may be measured using antibody staining of non- permeabilised cells followed by flow cytometry.

Activity of EP402R

In an embodiment of the attenuated ASFV of the invention, EP402R activity is disrupted.

In an embodiment the activity of EP402R that is disrupted is its ability to mediate haemadsorption. In other words, the ability of EP402R to mediate haemadsorption may be decreased. The ability of EP402R to mediate haemadsorption may be decreased by at least 50, 60, 70, 80 or 90%. The ability of EP402R to mediate haemadsorption may be completely abolished.

The activity of EP402R that is disrupted may be the ability of the EP402R protein to bind ligands via its extracellular N-terminal Ig-like domain (i.e. its ligand-binding domain). Thus in an embodiment the invention provides an attenuated ASF virus wherein the EP402R gene comprises one or more mutations that disrupt ligand binding by the EP402R protein. The ability of the EP402R protein to bind one or more ligands may be disrupted. The ability of EP402R to bind one or more ligands may be decreased by at least 50, 60, 70, 80 or 90%. The ability of the EP402R protein to bind one or more ligands may be completely abolished. The ability of the EP402R protein to bind one or more ligands may be completely abolished whilst retaining the ability to bind other ligands. In an embodiment, the invention provides an attenuated ASF virus wherein the EP402R gene comprises one or more mutations that disrupt ligand binding by the EP402R protein. Ligand binding may be measured using assays such as immunoprecipitation, surface plasmon resonance and/or isothermal calorimetry.

Functional version of a gene

The attenuated ASFV of the invention comprises a functional version of one or more of the following genes: multigene family (MGF) 1103L, 6L, 7L, 8L, 10L, 11 L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1R, 2R and 6R.

In some embodiments the attenuated ASFV of the invention may comprise a functional version of one or more of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L; MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R; and MGF 505 1R, 2R and 6R. Suitably, the attenuated ASFV comprises functional versions of two or more, such as three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, eleven or more, twelve or more, thirteen or more, fourteen or more, fifteen or more, sixteen or more, seventeen or more, eighteen or more, nineteen or more, twenty or more, twenty one, or twenty two of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11L and 12L; MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R; and MGF 505 1R, 2R and 6R. In an embodiment the attenuated ASFV comprises functional versions of all of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L; MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21 R and 22R; and MGF 505 1 R, 2R and 6R.

The expression “functional version” of a gene refers to a gene the expression and activity of which have not been disrupted. A functional version of a gene may not be mutated in a manner that disrupts gene expression or gene activity. A functional version of a gene may not comprise any mutations. The coding sequence of a functional version of a gene may be complete and uninterrupted. A functional version of a gene may be fully transcribed and translated. A functional version of a gene may correspond to the gene in a wild-type ASFV isolate. A functional version of a gene may correspond to the gene in a virulent ASFV strain. The sequence of a functional version of a gene may be identical to the sequence of the gene in a wild-type ASFV isolate or virulent ASFV strain. The sequence of a functional version of a gene may be identical to the sequence of the gene in the wild-type ASFV isolate from which the attenuated ASFV of the invention is derived. A functional version of a gene may be a natural variant of the gene in a wild-type ASFV isolate.

A functional version of a gene may comprise mutations. However, the mutations should not disrupt the expression or activity of the gene. In other words, the mutations should not affect the function of the gene. A functional version of a gene may comprise one or more synonymous mutations (i.e. mutations which do not alter the amino acid sequence of the protein the gene encodes). A functional version of a gene may comprise one or more silent mutations, which may be synonymous or non-synonymous. A functional version of a gene may comprise deletions that do not disrupt the expression or activity of the gene. A functional version of a gene may comprise one or more single nucleotide polymorphisms (SNPs) that do not disrupt the expression or activity of the gene.

Deletions

In the attenuated ASFV of the invention, expression and/or activity of genes may be disrupted by deletion. In other words, expression and/or activity of a gene may be disrupted by a mutation that is a deletion.

An attenuated ASFV of the invention may be made to lack a functional version of a gene by deletion. In other words, the mutation that causes the ASFV to lack a functional version of a gene may be a deletion.

“Deletion” means removal of part of the ASFV genome nucleotide sequence. The deletion may be continuous, or may comprise deletion of a plurality of sections of sequence. Deletion may disrupt gene expression and/or activity in any of the ways described herein. Deletion may cause the ASFV to lack a functional version of the gene in any of the ways described herein.

Deletion may disrupt transcription of the gene into mRNA. For example, deleting a promoter of a gene would disrupt transcription. Deletion may disrupt translation of mRNA into protein. For example, deleting a start codon would disrupt translation. Gene expression and/or activity may be disrupted by deleting non-coding sequence associated with the gene, such as a promoter. Gene expression and/or activity may be disrupted by deleting coding sequence of the gene. The ASFV may be made to lack a functional version of the gene by deleting coding sequence of the gene. Deletion of coding sequence may be partial (i.e. part of the coding sequence is deleted). The deletion may, for example, remove at least 50, 60, 70, 80 or 90% of the coding sequence of the gene. The deletion may be complete, in which case 100% of the coding sequence of the gene is absent when compared to the corresponding genome of the wild-type isolate.

Deletion of coding sequence may be continuous, or may comprise deletion of a plurality of sections of coding sequence. The deletion should remove a sufficient amount of coding sequence such that deletion disrupts the expression and/or activity of the gene i.e. a functional gene product, such as a protein, is no longer produced from the gene. The expression “deletion of a gene” (such as “partially deleted”) refers to deletion of a sufficient amount of coding sequence such that expression and/or activity of the gene is disrupted. The amount of coding sequence required to be deleted to disrupt gene expression and/or activity may be very small. For example, deletion of just the start codon (ATG) may be sufficient to disrupt expression and/or activity of the gene to attenuate the virus.

Partial and full deletions of a gene can be made using known techniques in the art, such as conditional targeting via Cre-LoxP and Flp-FRT systems, or by inducing a double strand break (DSB) and repair using engineered nucleases such as meganucleases, zinc finger nucleases (ZFNs), transcription-activator like effector nucleases (TALENs) and Cas in CRISPR-Cas systems. The DSB repair can be exploited to introduce a desired mutation by providing a vector comprising the desired mutated nucleotide sequence within a sequence that is homologous to the sequences flanking either side of the DSB. This results in the desired mutation being inserted at the site of the DSB. Nucleases such as those above can be engineered to induce DSB at a specific site within the genome. For example, chimeric meganucleases can be readily generated by combining known protein units to recognise a target recognition sequence within a gene or genomic region of interest. ZFNs can also be designed to target specific sequences, for example combining zinc-finger units with known specificities to bind specific regions of DNA. TALENs are artificial restriction enzymes designed by fusing a nuclease domain to DNA-binding TALE (transcription activator- 1 ike effector) domains. TALE domains are tandem arrays of amino acid repeats that recognise a single nucleotide and can be designed to target a specific region of DNA. CRISPR-Cas systems consist of a Cas (CRISPR-associated protein) nuclease and a CRISPR (clustered regularly interspaced short palindromic repeat) RNA sequence that guides the Cas protein to recognise and cleave a specific strand of DNA complementary to the CRISPR sequence. Single-stranded guide RNA (sgRNA) can therefore be designed to bind to a specific region of DNA and guide the Cas to introduce a DSB. Accordingly, provided the nucleotide (e.g. DNA or cDNA) sequence of a gene is known, a known nuclease system can be utilised to introduce a partial or full deletion to the gene.

In an embodiment of the attenuated ASF virus of the present invention the genes DP148R, EP153R and EP402R may be at least partially deleted.

The DP148R gene may be at least partially deleted. The EP153R gene may be at least partially deleted. The EP402R gene may be at least partially deleted. The DP148R and EP153R genes may each be at least partially deleted. The DP148R, EP153R and EP402R genes may each be at least partially deleted.

In an embodiment of the attenuated ASF virus of the present invention the genes DP148R, EP153R and EP402R may be completely deleted.

The DP148R gene may be completely deleted. The EP153R gene may be completely deleted. The EP402R gene may be completely deleted. The DP148R and EP153R genes may each be completely deleted. The DP148R, EP153R and EP402R genes may each be completely deleted.

Interruptions

The expression and/or activity of the genes may be disrupted by interruption of the gene. In other words, the mutation that disrupts expression and/or activity of a gene may be one that interrupts the gene.

In an embodiment of the attenuated ASFV of the present invention the genes DP148R, EP153R and EP402R may interrupted. The DP148R gene may be interrupted. The EP153R gene may be interrupted. The EP402R gene may be interrupted. The DP148R and EP153R genes may each be interrupted. The DP148R, EP153R and EP402R genes may each be interrupted.

“Interruption” means the mutation alters the coding sequence of the gene such that a functional gene product, such as a protein, is no longer produced. The term “interruption” may be used herein to refer to a mutation that interrupts a gene. The mutation(s) should interrupt the coding sequence in a manner such that expression and/or activity of the gene is disrupted i.e. a functional gene product, such as a protein, is no longer produced from the gene.

The interruption may entirely abolish gene product production. For example, where the gene product is a protein, the interruption may render the mRNA nonsensical, causing the mRNA to be degraded and the protein to not be translated, thereby abolishing protein production. The interruption may alter the gene product that is produced. The interruption may cause the gene to not be transcribed and/or translated.

The interruption may be a point mutation (i.e. substitution, insertion or deletion of a single nucleotide). An interruption may be a deletion. A gene may comprise multiple mutations that lead to interruption of the gene.

The interruption may be a frame shift mutation. A frame shift causes the codons downstream of the frame shift to be read as different amino acids. The protein produced may be non- functional.

The interruption may be mutation of a start codon. A start codon is typically ATG. Mutation of a start codon (e.g. point mutation of one, two or three of the nucleotides) means that translation will not start at that codon. Translation may begin at a subsequent start codon further downstream. If the subsequent start codon is in frame a version of the protein is produced that is N-terminally truncated and so may be non-functional. If the subsequent start codon is not in frame an entirely different or nonsense protein is produced, which would be non-functional. If there is no subsequent start codon, translation is entirely abolished and no protein is produced.

The interruption may be mutation of a stop codon (TAG, TAA or TGA). Mutation of a stop codon (also referred to as a nonstop mutation) causes continued translation of mRNA into a sequence that should not be translated. The resulting protein may be non-functional due to its excessive length.

Amino acid changes

In an embodiment of the attenuated ASF virus of the invention, the EP402R gene comprises one or more mutations that change one or more amino acids in the ligand-binding domain of the EP402R protein. Suitably the EP402R gene comprises one or more mutations (such as two or more, three or more, four or more or five or more mutations) that change one or more amino acids (such as two or more, three or more, four or more or five or more amino acids) in the ligand-binding domain of the EP402R protein.

Changing of the one or more amino acids in the ligand-binding domain of the EP402R protein should disrupt expression and/or activity of the EP402R protein. In an embodiment, changing the one or more amino acids in the ligand-binding domain of the EP402R protein may disrupt haemadsorption mediated by the EP402R protein. In an embodiment, changing the one or more amino acids in the ligand-binding domain of the EP402R protein may disrupt ligand binding by the EP402R protein.

In an embodiment, the one or more mutations in the ligand-binding domain may be a deletion or an interruption as described herein. For example, deletion of part of the coding sequence for the ligand-binding domain of the EP402R protein would result in the absence of the encoded amino acids from the ligand-binding domain, which may disrupt ligand binding.

In an embodiment, one or more of the mutations may be a point mutation. Suitably one or more of the mutations may be point mutations that change a single amino acid into a different amino acid. Such an amino change may be referred to as a substitution. Changing even a single amino acid may disrupt the activity of the EP402R protein, such as ligand binding activity. For example, changing an amino acid that can be charged to an amino acid that can have the opposite charge may disrupt folding of the EP402R ligand-domain such that a binding pocket is deformed, which prevents ligand binding. Alternatively or additionally, substitution with an amino acid that can have an opposite charge may prevent electrostatic binding of a ligand. Another possibility is that changing an amino acid with a small side-chain to an amino acid with a bulky side-chain, or vice versa, may disrupt folding of the EP402R ligand-binding domain so that a binding pocket does not form which prevents ligand binding.

Thus in an embodiment the one or more changed amino acids in the ligand-binding domain may comprise a negatively charged amino acid that is changed to a positively charged amino acid. In an embodiment the one or more changed amino acids in the ligand-binding domain may comprise a positively charged amino acid that is changed to a negatively charged amino acid. Positively charged amino acids (i.e. amino acids that can have a positive charge) include histidine (H), lysine (K) and arginine (R). Negatively charged amino acids (i.e. amino acids that can have a negative charge) include aspartic acid (D) and glutamic acid (E).

In an embodiment the one or more changed amino acids in the ligand-binding domain may comprise an amino acid with a small side-chain that is changed to an amino acid with a bulky side-chain. In an embodiment the one or more changed amino acids in the ligand- binding domain may comprise an amino acid with a bulky side-chain that is changed to an amino acid with a small side-chain. Amino acids with a bulky side-chain include tryptophan (W). Amino acids with a small side-chain include glycine (G) and alanine (A). In an embodiment the one or more changed amino acids in the ligand-binding domain may comprise an amino acid with a hydrophilic side-chain that is changed to an amino acid with a hydrophobic side-chain. In an embodiment the one or more changed amino acids in the ligand-binding domain may comprise an amino acid with a hydrophobic side-chain that is changed to an amino acid with a hydrophilic side-chain. Amino acids with a hydrophobic side-chain include alanine (A), valine (V), isoleucine (I), leucine (L), methionine (M), phenylalanine (F), tyrosine (Y) and tryptophan (W). Amino acids with a hydrophilic side-chain include arginine (R), histidine (H), lysine (K), aspartic acid (D), glutamic acid (E), serine (S), threonine (T), asparagine (N) and glutamine (Q).

Changing amino acid E99 to R or amino acid Y102 to D disrupts the ability of EP402R protein from Benin 97/1 strain to mediate haemadsorption (see Figures 4, 5 and 6). Furthermore, changing the amino acid Q96 to R disrupts the ability of EP402R protein from Georgia 2007/1 strain to mediate haemadsorption (see Figure 4C). Accordingly, in an embodiment the one or more mutations change an amino acid at a position in the EP402R protein which corresponds to E99 and/or Y102 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In other words, the EP402R protein expressed by the attenuated ASF virus of the invention may be the EP402R protein from any ASF virus strain, and the amino acid that is changed corresponds to E99 and/or Y102 of the amino acid sequence of the EP402R protein from the Benin 97/1 strain, which is presented herein as SEQ ID NO. 11. The positions E99 and Y102 refer to amino acids glutamic acid and tyrosine at positions 99 and 102 respectively of the Benin 97/1 EP402R protein. In an embodiment the one or more mutations change the amino acid at position E99 and/or Y102 of the EP402R protein of the Benin 97/1 strain, or the corresponding position in the EP402R protein of any other ASFV strain. In an embodiment the one or more mutations change amino acids at positions in the EP402R protein which corresponds to E99 and Y102 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change an amino acid at a position in the EP402R protein corresponding to E99 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change an amino acid at a position in the EP402R protein which corresponds to Y102 of the Benin 97/1 EP402R protein (SEQ ID NO. 11).

Whether an amino acid corresponds to E99 and/or Y102 of the Benin 97/1 EP402R protein may be assessed by sequence alignment. For example, Figure 3 shows an alignment of EP402R proteins from Benin, Mkuzi, Warmbaths, Tengnani, Warthog, Georgia and Malawi strains. The amino acid in the EP402R protein from each strain that corresponds to E99 of Benin 97/1 EP402R protein is the amino acid shown below the Benin E99 residue (highlighted in yellow). The amino acid in the EP402R protein from each strain that corresponds to Y102 of Benin 97/1 EP402R protein is the amino acid shown below the Benin Y102 residue (three amino acids to the right of the amino acids highlighted in yellow). The amino acids in the EP402R protein from the strains in Figure 3 that corresponds to E99 and Y102 in Benin 97/1 EP402R are given in Table 4 below.

Table 4

Amino acids corresponding to E99 and Y102 of Benin 97/1 EP402R exist in EP402R proteins from strains other than those shown in Figure 3 and listed in Table 4 above (such as other strains listed in Table 1 herein). The skilled person can readily determine using sequence alignment which amino acid in an EP402R protein from a given strain, such as a strain from Table 1, corresponds to E99 or Y102 of Benin 97/1 EP402R. The corresponding amino acid may be identical to E99 or Y102 of Benin 97/1 EP402R. For example, an amino acid corresponding to E99 of Benin 97/1 EP402R may also be an E (glutamic acid). The corresponding amino acid may be similar to E99 or Y102. Suitably the corresponding amino acid has the same charge as E99 or Y102. Suitably the corresponding amino acid to E99 is a Q (glutamine). Suitably the corresponding amino acid to Y102 is a W (tryptophan). The expression “corresponding to E99 and/or Y102 of Benin 97/1 EP402R protein” encompasses E99 and/or Y102D of Benin 97/1 EP402R protein.

In an embodiment the one or more mutations change an amino acid at position E99 and/or Y102 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change an amino acid at position E99 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change an amino acid at position Y102 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change an amino acid at position Q96 of Georgia 2007/1 EP402R protein (SEQ ID NO. 24).

In an embodiment the one or more mutations change an amino acid at a position in the EP402R protein corresponding to N16, 119, W21, Y76, E99, Y102 and/or N108 of Benin 97/1 EP402R protein (SEQ ID NO. 11). In an embodiment the one or more mutations change the amino acid at position N 16, 119, W21, Y76, E99, Y102 and/or N108 of the EP402R protein of the Benin 97/1 strain, or the corresponding position in the EP402R protein of any other ASFV strain. In an embodiment the one or more mutations change an amino acid at a position in the EP402R protein corresponding to S15, W19, Q96, N104, and/or K108D of Georgia 2007/1 EP402R protein (SEQ ID NO. 24). In an embodiment the one or more mutations change the amino acid at position S15, W19, Q96, N104, and/or K108D of the EP402R protein of the Georgia 2007/1 EP402R protein (SEQ ID NO. 24), or the corresponding position in the EP402R protein of any other ASFV strain. In an embodiment the one or more mutations change an amino acid at a position in the EP402R protein corresponding to W20, Q112, N121 and/or R125 of N10 Genotype IX EP402R protein (SEQ ID NO. 27). In an embodiment the one or more mutations change the amino acid at position S15, W19, Q96, N104, and/or K108D of the EP402R protein of the N10 Genotype IX EP402R protein (SEQ ID NO. 27), or the corresponding position in the EP402R protein of any other ASFV strain. These amino acids are in the ligand-binding domain of the EP402R protein and are surface exposed.

Suitably, the mutation is selected from N16R, I19R, W21D, Y 76D, E99R, Y102D and/or N108R at a position corresponding to the position in the Benin 97/1 EP402R protein (SEQ ID NO. 11). Suitably, the mutation is a combination of E99R and N108R at the positions corresponding to the positions in the Georgia 2007/1 EP402R protein (SEQ ID NO. 11).

Suitably, the mutation is selected from S15R, W19D, Q96R, N104R and/or K108D at a position corresponding to the position in the Georgia 2007/1 EP402R protein (SEQ ID NO. 24). Suitably, the mutation is a combination of Q96R and N104R at the positions corresponding to the positions in the Georgia 2007/1 EP402R protein (SEQ ID NO. 24).

Suitably, the mutation is selected from W20D, Q112R, N121R and/or R125D at a position corresponding to the position in the N10 Genotype IX EP402R protein (SEQ ID NO. 27). Suitably, the mutation is a combination of Q112R and N121R at the positions corresponding to the positions in the N10 Genotype IX EP402R protein (SEQ ID NO. 27).

Suitably, where the attenuated ASFV is genotype I, the one or more mutations change an amino acid at a position in the EP402R protein corresponding to Y76, E99, Y102, and/or N 108 of Benin 97/1 EP402R protein (SEQ ID NO. 11). Suitably, where the attenuated ASFV is genotype I, the mutation is selected from Y76D, E99R, Y102D, and/or N108R at a position corresponding to the position in the Benin 97/1 EP402R protein (SEQ ID NO. 11).

Suitably, where the attenuated ASFV is genotype II, the one or more mutations change an amino acid at a position in the EP402R protein corresponding to S15, W19, Q96, N104, and/or K108D of Georgia 2007/1 EP402R protein (SEQ ID NO. 24). Suitably, where there attenuated ASFV is genotype II, the mutation is selected from S15R, W19D, Q96R, N104R and/or K108D at a position corresponding to the position in the Georgia 2007/1 EP402R protein (SEQ ID NO. 24). Suitably, where the attenuated ASFV is genotype II, the mutation may be a combination of Q96R and N104R at the positions corresponding to the positions in the Georgia 2007/1 EP402R protein (SEQ ID NO. 24).

In an embodiment the amino acid at the position corresponding to E99 is changed to R and/or the amino acid at the position corresponding to Y102 is changed to D. In an embodiment the amino acid at the position corresponding to E99 is changed to R. In an embodiment the amino acid at the position corresponding to Y102 is changed to D. In an embodiment E99 of Benin 97/1 EP402R is changed to R and/or Y102 of Benin 97/1 EP402R is changed to D. In an embodiment E99 of Benin 97/1 EP402R is changed to R. In an embodiment Y102 of Benin 97/1 EP402R is changed to D. In an embodiment Q96 of Georgia 2007/1 EP402R is changed to R.

The amino acid sequence of the EP402R protein from Benin 97/1 strain comprising the E99R substitution is depicted below as SEQ ID No. 31.

SEQ ID No. 31 - Benin 97/1 EP402R E99R protein

MilIVIFLMCLKIVLNNIIIWSTLNQTVFLNNIFTINDTYGGLFWNTYYDNNRSNFT YCGIAGNYCSCCGHNISLYNTTNNCS LIIFPNNTEIFNRTYRLVYLDKKINYTVKLLKSVDSPTITYNCTNSLITCKNNNGTNVNI YLIINNTIVNDTNGDILNYYWNG NNNFTATCMINNTISSLNETENINCTNPILKYQNYLSTLFYIIIFIVSGLIIGIFISIIS VLSIRRKRKKHVEEIESPPPSES NEEDISHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPPPKPCSPPK PCRPPKPCPPPKPCPPPKPCPPPKPCPPSKPCPSPESYSPPKPLPSIPLLPNIPPLSTQN ISLIHVDRII

In an embodiment the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 31. Suitably, the EP402R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 31, wherein amino acid 99 is R. Suitably, the EP402R gene encodes a protein consisting of the sequence of SEQ ID No. 31.

The amino acid sequence of the EP402R protein from Benin 97/1 strain comprising the Y102D substitution is depicted below as SEQ ID No. 32.

SEQ ID No. 32 - Benin 97/1 EP402R Y102D protein

MIIIVIFLMCLKIVLNNIIIWSTLNQTVFLNNIFTINDTYGGLFWNTYYDNNRSNFT YCGIAGNYCSCCGHNISLYNTTNNCS LIIFPNNTEIFNRTYELVDLDKKINYTVKLLKSVDSPTITYNCTNSLITCKNNNGTNVNI YLIINNTIVNDTNGDILNYYWNG NNNFTATCMINNTISSLNETENINCTNPILKYQNYLSTLFYIIIFIVSGLIIGIFISIIS VLSIRRKRKKHVEEIESPPPSES NEEDISHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPPPKPCSPPK PCRPPKPCPPPKPCPPPKPCPPPKPCPPSKPCPSPESYSPPKPLPSIPLLPNIPPLSTQN ISLIHVDRII

In an embodiment the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 32. Suitably, the EP402R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 32, wherein amino acid 102 is D. Suitably, the EP402R gene encodes a protein consisting of the sequence of SEQ ID No. 32.

The amino acid sequence of the EP402R protein from Georgia 2007/1 strain comprising the Q96R substitution is depicted below as SEQ ID No. 33.

SEQ ID No. 33 - Georgia 2007/1 EP402R Q96R protein

MIILIFLIFSNIVLSIDYWVSFNKTIILDSNITNDNNDINGVSWNFFNNSFNTLATC GKAGNFCECSNYSTSIYNITNNCSLT IFPHNDVFDTTYRWWNQIINYTIKLLTPATPPNITYNCTNFLITCKKNNGTNTNIYLNIN DTFVKYTNESILEYNWNNSNIN NFTATCIINNTISTSNETTLINCTYLTLSSNYFYTFFKLYYIPLSIIIGITISILLISII TFLSLRKRKKHVEEIESPPPESN EEEQCQHDDTTSIHEPSPREPLLPKPYSRYQYNTPIYYMRPSTQPLNPFPLPKPCPPPKP CPPPKPCPPPKPCPSAESYSPPK PLPSIPLLPNIPPLSTQNISLIHVDRII

In an embodiment the EP402R gene encodes a protein comprising the sequence of SEQ ID No. 33. Suitably, the EP402R gene encodes a protein comprising a sequence having at least 70%, at least 80%, at least 90% or at least 95% identity with SEQ ID No. 33, wherein amino acid 96 is R. Suitably, the EP402R gene encodes a protein consisting of the sequence of SEQ ID No. 33. In an embodiment the EP402R gene encodes a protein comprising a sequence selected from SEQ ID No. 31, 32 or 33.

Mutation combinations

The mutations that disrupt gene expression and/or activity described herein may be combined in an attenuated ASFV of the invention. In other words, each of the DP148R, EP153R and EP402R genes in an attenuated ASFV of the invention may be disrupted by the same type of mutation as any of the other genes or by a different type of mutation as any of the other genes.

For example, in an attenuated ASFV of the invention, DP148R may be disrupted by mutation of a promoter sequence, EP153R may be disrupted by complete deletion and EP402R may be disrupted by an amino acid change in its ligand-binding domain. As an alternative example, DP148R may be disrupted by partial deletion, EP153R may be disrupted by interruption and EP402R may be disrupted by complete deletion.

In an embodiment the invention provides an attenuated ASFV in which the DP148R gene and EP153R gene are each completely deleted, and the EP402R gene comprises a mutation that changes an amino acid at a position in the EP402R protein which corresponds to E99 of Benin 97/1 EP402R protein (SEQ ID No. 11) to R; and which comprises a functional version of one or more of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1R, 2R and 6R.

The attenuated ASFV may comprise a functional version of one or more of the MGF genes as described herein.

In an embodiment the invention provides an attenuated ASFV in which the DP148R gene and EP153R gene are each completely deleted, and the EP402R gene comprises a mutation that changes an amino acid at a position in the EP402R protein which corresponds to E99 of Benin 97/1 EP402R protein (SEQ ID No. 11) to R; and which comprises a functional version of one or more of the following genes:

MGF 110 3L, 6L, 7L, 8L, 10L, 11L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1R, 2R and 6R.

The attenuated ASFV may comprise a functional version of one or more of the MGF genes as described herein.

In an embodiment the invention provides an attenuated ASFV in which the DP148R gene and EP153R gene are each completely deleted, and the EP402R gene comprises a mutation that changes an amino acid at a position in the EP402R protein equivalent to Y102 of Benin 97/1 EP402R protein (SEQ ID No. 11) to D; and which comprises a functional version of one or more of the following genes:

MGF 110 3L, 6L, 7L, 8L, 10L, 11L and 12L,

MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21 R and 22R, and MGF 505 1R, 2R and 6R.

The attenuated ASFV may comprise a functional version of one or more of the MGF genes as described herein.

Vaccine/pharmaceutical composition

The present invention also provides a vaccine comprising an attenuated ASF virus of the invention. The term “vaccine” as used herein refers to a preparation which, when administered to a subject, induces or stimulates a protective immune response. A vaccine can render an organism immune to a particular disease, in the present case ASF. The vaccine of the present invention thus induces an immune response in a subject which is protective against subsequent ASF virus challenge. A vaccine comprising an attenuated ASFV of the invention may be capable of inducing a cross-protective immune response against a plurality of ASF virus genotypes. In an embodiment a vaccine comprising an attenuated ASFV of the invention of a single genotype may be capable of inducing a cross-protective immune response against a plurality of ASF virus genotypes.

The vaccine may comprise a plurality of attenuated ASF viruses. The plurality of attenuated ASF viruses may correspond to a plurality of different isolates, for example, different isolates of high or unknown virulence. Such a vaccine may be capable of inducing a cross-protective immune response against a plurality of ASF virus genotypes.

The vaccine may be useful in preventing African Swine Fever. Accordingly the invention provides a vaccine of the invention for use in treating and/or preventing African Swine Fever in a subject.

The present invention also provides a pharmaceutical composition which comprises one or more attenuated ASF virus(es) of the invention. The pharmaceutical composition may be used for treating African Swine Fever.

The vaccine or pharmaceutical composition may comprise one or more attenuated ASF virus(es) of the invention and optionally one or more adjuvants, excipients, carriers and diluents. The choice of pharmaceutical excipient, carrier or diluent can be selected with regard to the intended route of administration and standard pharmaceutical practice. The pharmaceutical compositions may comprise as (or in addition to) the carrier, excipient or diluent, any suitable binder(s), lubricant(s), suspending agent(s), coating agent(s), solubilising agent(s) and other carrier agents. The pharmaceutical compositions typically should be sterile and stable under the conditions of manufacture and storage. Formulations for parenteral administration include, but are not limited to, suspensions, solutions, emulsions in oily or aqueous vehicles, pastes, and implantable sustained-release or biodegradable formulations. Sterile injectable formulations may be prepared using a non- toxic parenterally acceptable diluent or solvent. A pharmaceutical composition of the present invention may include pharmaceutically acceptable dispersing agents, wetting agents, suspending agents, isotonic agents, coatings, antibacterial and antifungal agents, carriers, excipients, salts, or stabilizers which are nontoxic to the subjects at the dosages and concentrations employed. Preferably, such a composition can further comprise a pharmaceutically acceptable carrier or excipient for use in the treatment of disease that that is compatible with a given method and/or site of administration, for instance for parenteral (e.g. sub-cutaneous, intradermal, or intravenous injection) or intrathecal administration.

The vaccine or pharmaceutical composition may comprise one or more attenuated ASF virus(es) of the invention in an effective amount.

In an embodiment the invention provides an attenuated ASF virus of the invention which when administered to a subject induces an immune response which is protective against subsequent challenge with virulent ASF virus. In an embodiment the invention provides an attenuated ASF virus of the invention which when administered to a subject induces an immune response which is protective against subsequent challenge with virulent ASF virus of a different genotype to the attenuated ASF virus of the vaccine.

Methods of prevention/treatment

The present invention also provides a method of preventing and/or treating ASF in a subject by administration to the subject of an effective amount of an attenuated virus, vaccine, or pharmaceutical composition of the invention.

The term “preventing” is intended to refer to averting, delaying, impeding or hindering the contraction of ASF. The vaccine may, for example, prevent or reduce the likelihood of an infectious ASFV entering a cell.

The term “treating” is intended to refer to reducing or alleviating at least one symptom of an existing ASF infection.

The subject may be any animal which is susceptible to ASF infection. ASF susceptible animals include domestic pigs, warthogs, bush pigs and ticks.

The subject vaccinated according to the present invention may be a domestic pig.

Administration

The vaccine of the invention may be administered by any convenient route, such as by intramuscular injection. Other suitable routes of administration include intranasal, oral, subcutaneous, transdermal and vaginal (e.g. during artificial insemination). In one embodiment, oral administration comprises adding the vaccine to animal feed or drinking water. In another embodiment, the vaccine may be added to bait for a wild animal, for example bait suitable for wild boar, wild pigs, bushpigs or warthogs. The dose for pig immunisation may be less than 10 ⁴ HAD ₅₀ or TCID ₅₀ per pig. For example the dose may be between 10 ² - 10 ³ HAD ₅₀ or TCID _50. The dose may be about 10 ² HAD ₅₀ or TCID ₅₀ per pig. The dose may be determined by a veterinary practitioner within the scope of sound veterinary judgment.

The vaccine may be administered following a prime-boost regime. For example, after the first inoculation, the subjects may receive a second boosting administration some time (such as about 7, 14, 21 or 28 days) later. Typically the boosting administration is at a higher dose than the priming administration. The boosting dose may be about 10 ² , 10 ³ or 10 ⁴ HAD ₅₀ or TCID ₅₀ of the recombinant attenuated virus per pig.

Method for preparing an attenuated virus

The present invention also provides a method of attenuating an ASF virus which comprises the step of disrupting the expression and/or activity of the genes DP148R, EP153R and EP402R.

Disruption of gene expression and/or activity may be achieved by mutating the ASFV genome in any of the ways described herein.

The DP148R, EP153R and/or EP402R genes may be partially or completely deleted.

The DP148R gene may be at least partially or completely deleted. The EP153R gene may be at least partially or completely deleted. The EP402R gene may be at least partially or completely deleted. The DP148R and EP153R genes may be at least partially or completely deleted. The DP148R, EP153R and EP402R genes may each be partially or completely deleted. The coding sequences of one or more (such as two or more, or all three) of the disrupted genes may be at least partially or completely deleted.

The DP148R, EP153R and/or EP402R genes may be interrupted.

The DP148R gene may be interrupted. The EP153R gene may be interrupted. The EP402R gene may be interrupted. The DP148R and EP153R genes may be interrupted. The DP148R, EP153R and EP402R genes may each be interrupted. The coding sequences of one or more (such as two or more, or all three) of the disrupted genes may be interrupted.

In an embodiment the method of attenuating an ASF virus comprises disrupting the ability of the EP402R gene to mediate haemadsorption. In an embodiment the method of attenuating an ASF virus comprises comprising introducing one or more mutations in the EP402R gene that disrupt ligand binding by the EP402R protein.

In an embodiment the method of attenuating an ASF virus comprises introducing one or more mutations in the EP402R gene that change one or more amino acids in the ligand- binding domain of the EP402R protein. The mutation may be one or more of the mutation as described herein.

In an embodiment the method of attenuating an ASF virus comprises changing the amino acid at position R96, E99 and/or Y102 of the EP402R protein.

In an embodiment the method of attenuating an ASF virus comprises changing the amino acid at position R96 to Q, the amino acid at position E99 to R and/or the amino acid at position Y102 to D. Suitably the method comprises changing the amino acid at position R96 to Q. Suitably the method comprises changing the amino acid at position E99 to R. Suitably the method comprises changing the amino acid at position Y102 to D.

In an embodiment the method of attenuating an ASF virus comprises completely deleting the DP148R gene, completely deleting the EP153R gene and introducing a mutation in the EP402R gene that changes the amino acid at position R96 of the EP402R protein to Q. In an embodiment the method of attenuating an ASF virus comprises completely deleting the DP148R gene, completely deleting the EP153R gene and introducing a mutation in the EP402R gene that changes the amino acid at position E99 of the EP402R protein to R. In an embodiment the method of attenuating an ASF virus comprises completely deleting the DP148R gene, completely deleting the EP153R gene and introducing a mutation in the EP402R gene that changes the amino acid at position Y102 of the EP402R protein to D.

Methods for mutation of viral genes are known in the art. In particular, methods for deletion of viral genes are known in the art. For example, homologous recombination may be used, in which a transfer vector is created in which the relevant gene(s) are missing and used to transfect virus-infected cells. Recombinant viruses expressing the new portion of sequence may then be selected. Similar procedures may be used in order to interrupt gene expression, for example by deletion of the ATG start codon.

“Retaining the function” of a gene means that expression and activity of the gene is not affected during the attenuation process. The resultant attenuated virus should express a functional version of the gene. Suitably, the genes the function of which is to be retained are unaltered by the method of attenuation. Suitably, the sequences of the genes the function of which is to be retained are unaltered by the method of attenuation.

In some embodiments, the method of attenuating an ASF virus may comprise retaining the function of one or more of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L; MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R; and MGF 505 1R, 2R and 6R. Suitably, the function of two or more, such as three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, eleven or more, twelve or more, thirteen or more, fourteen or more, fifteen or more, sixteen or more, seventeen or more, eighteen or more, nineteen or more, twenty or more, twenty one, or twenty two of the following genes are retained: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L; MGF 360 5L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R; and MGF 505 1R, 2R and 6R. In an embodiment the method of attenuating an ASF virus may comprise retaining the function of all of the following genes: MGF 110 3L, 6L, 7L, 8L, 10L, 11 L and 12L; MGF 3605L, 6L, 7L, 10L, 11 L, 12L, 13L, 14L, 20R, 21R and 22R; and MGF 505 1R, 2R and 6R.

This disclosure is not limited by the exemplary methods and materials disclosed herein, and any methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of this disclosure. Numeric ranges are inclusive of the numbers defining the range. Unless otherwise indicated, any nucleic acid sequences are written left to right in 5' to 3' orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively.

It must be noted that as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise.

The terms "comprising", "comprises" and "comprised of as used herein are synonymous with "including", "includes" or "containing", "contains", and are inclusive or open-ended and do not exclude additional, non-recited members, elements or method steps. The terms "comprising", "comprises" and "comprised of also include the term "consisting of.

The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that such publications constitute prior art to the claims appended hereto.

The invention will now be further described by way of Examples, which are meant to serve to assist one of ordinary skill in the art in carrying out the invention and are not intended in any way to limit the scope of the invention.

EXAMPLES Example 1 - Identification of EP402R/CD2v mutants that reduce HAD

Mutations were made in African swine fever virus (ASFV) Benin isolate EP402R protein (CD2v) and tested for their effect on haemadsorption (HAD).

A model of the extracellular, N-terminal, IgG-like, ligand-binding domain of CD2v was generated and used to predict the functional amino acid residues involved in binding of CD2v to its ligand. These residues were individually mutated to generate a set of mutant CD2v proteins.

Vero cells were infected with modified vaccinia virus Ankara expressing T7RNA polymerase and transfected with plasmids (pcDNA3) expressing wild-type or mutant CD2v full-length proteins with a C-terminal HA epitope tag. Pig red blood cells were added and cells observed for attachment of red blood cells to the surface. Expression of the wild-type or mutant CD2v proteins was confirmed both by confocal microscopy using permeabilised cells and Western blotting using an antibody recognising the HA tag and a secondary antibody. Cell surface expression of the wild-type or mutant CD2v was also confirmed by staining non- permeabilised cells with sera from pigs immunised with attenuated ASFV containing a wild- type CD2v gene and a secondary antibody (Figure 1).

Mutation of residues E99 or Y102 abrogated HAD. Figure 2 shows exemplary images from the HAD assay. Figure 2A shows cells transfected with a plasmid expressing wild-type Benin CD2v. HAD of red blood cells is observed around three cells. Figure 2B shows cells expressing CD2v with the Y102 residue mutated to D. Partial HAD is observed around one cell. Figure 2C shows cells expressing CD2v with residue E99 mutated to R. No HAD is observed.

CD2v residue E99 is strongly conserved in ASFV, as shown by an alignment of the amino acid sequence of CD2v ligand-binding domain from different ASFV isolates of varying genotypes (Figure 3). The equivalent residue in other isolates (highlighted in yellow in Figure 3) is either identical (E) or has the same charge (Q).

The equivalent residue to Benin CD2v E99 in Georgia CD2v, Q96, was mutated to R and the ability of the mutant protein to induce HAD was tested using the HAD assay described above. Mutation of Q96 in Georgia CD2v abrogated HAD. Figure 4 shows exemplary images from the HAD assay of Vero cells with pig red blood cells. Figure 4A shows Vero cells transfected with a plasmid expressing wild-type Benin CD2v. HAD of red blood cells is observed around four cells. Figure 4B shows Vero cells expressing wild-type Georgia CD2v. HAD is observed around two cells. Figure 4C shows Vero cells expressing Georgia CD2v with residue Q96 mutated to R. No HAD is observed. Figure 4D shows non-transfected Vero cells. No HAD is observed.

Using the same assay, the following mutations were determined to impact HAD in Benin: N16R, I 19R, W21D, Y 76D, E99R, Y102D. In addition, a combination of E99R + N108R was determined to impact HAD in Benin.

The following mutations were determined to impact HAD in Georgia: S15R, W19D, Q96R, N104R, and K108D. In addition, combinations of S15R + W19D and Q96R + N104R were determined to impact HAD in Georgia.

The following mutations were determined to impact HAD in N10 Genotype IX: W20D, R125D, Q112R + N121R.

Example 2 - Generation of ASFV lacking functional DP148R, EP153R and EP402R

The ability to induce HAD could be restored by infecting WSL cells expressing the CD2vE99R mutant with BeninΔCD2v virus (Figure 4A).

However, HAD could not be restored by infecting WSL cells expressing the CD2vE99R mutant with OURT88/3 virus (Figure 4B). OURT88/3 has frame-shift interruptions in the EP153R and EP402R/CD2v genes, which suggests a role for EP153R in rescuing HAD caused by the CD2vE99R mutant.

Based on these results, two gene deleted Benin viruses were constructed using as backbone the attenuated BeninΔDP148R virus. One virus had in addition to DP148R further deletions of EP153R and EP402R (BeninΔDP148RΔEP153RΔCD2v) and the second had EP153R deleted and the wild-type EP402R gene replaced with the gene expressing CD2vE99R mutant (BeninΔDP148RΔEP153R-CD2vE99R).

The Benin CD2vE99R mutant protein was expressed at the cell surface, as shown in Figure 6A. Porcine bone marrow cells were infected with BeninΔDP148RΔEP153R-CD2vE99R, fixed and permeabilised at 8 hours post-infection then stained with an antibody which recognises the HA-tag fused in frame with the CD2vE99R gene and secondary antibody. The CD2vE99R is shown in red on the cell surface. The mNeon green was inserted in place of the EP153R gene and is shown in green. Nuclear DNA and virus cytoplasmic factories are stained blue. The panel on the right shows a larger field to indicate numbers of infected cells.

Cells infected with these viruses did not induce HAD. Figure 6B shows porcine bone marrow cells infected with wild type Benin97/1 virus (left panel) or BeninΔDP148RΔEP153R- CD2vE99R (right panel). Red blood cells are observed forming rosettes around the Benin97/1 infected cells (left panel) but not around the cells infected with virus expressing mutant CD2vE99R (right panel). The inset bottom right shows cells infected with BeninΔDP148RΔEP153R-CD2vE99R expressing mNeon protein to confirm the cells were infected.

Example 3 - DP148R inhibits activation of NF-kB by inhibiting nuclear translocation of the p65 sub-unit

HEK293T cells were transfected with an NF-kB dependent luciferase reporter plasmid and either a plasmid encoding the DP148R gene or an empty plasmid (pcDNA3) as a negative control. Twenty four hours post transfection the cells were treated with I L-1 β (Figure 7A) or TNFα (Figure 7B) for eight hours to induce NF-kB activation. Following cell lysis, luciferase activity was measured. Figure 7A and Figure 7B show that in the presence of DP148R, luciferase values were significantly lower than those obtained from cells transfected with pcDNA3. This indicates that DP148R is able to inhibit NF-KB mediated signalling.

In a pair of alternative experiments, NF-kB activation was instead induced by overexpression of the two main kinases of the NF-kB signalling pathway, namely IKKa (Figure 7C) and IKKb (Figure 7D). Figure 7C and Figure 7D show that, again, in the presence of DP148R, luciferase values were significantly lower than those obtained from cells transfected with pcDNA3, indicating that DP148R is able to inhibit the NF-kB pathway at the level of the kinases activation.

To further elucidate the mechanism by which DP148R inhibits NF-kB activation, the effect of DP148R on nuclear translocation of the p65 sub-unit of NF-kB was investigated. HeLa cells were transfected with a plasmid expressing HA-tagged DP148R and were then stimulated with TNFα lpha or I L-1 β to activate NF-kB. Unstimulated cells were used as a negative control. Cells were fixed and permeabilised then stained for HA-tagged DP148R using an anti-HA tag antibody and an appropriate secondary antibody. The p65 subunit of NF-kB was stained with a primary antibody and secondary antibody. Figure 8 shows immunofluorescence images of the cells. HA-tagged DP148R is shown in red, p65 is shown in green and nuclei are shown in blue. In cells not expressing DP148R, in unstimulated cells p65 remains in the cytoplasm but translocates into the nucleus following stimulation with TNFα lpha or IL-1beta. In contrast, in cells expressing DP148R, p65 nuclear translocation is not observed upon stimulation and lower amounts of p65 are detected in the cytoplasm. The results show that DP148R is an efficient inhibitor of NF-kB which would inhibit activation of genes dependent on NF-kB. These include for example proinflammatory cytokines and chemokines. Example 4 - Lack of functional DP148R, EP153R and EP402R attenuates ASFV and induces protection against challenge

Vaccination experimental protocol

Groups of large white/landrace pigs were immunised intramuscularly with 10 ⁴ TCID50 BeninΔDP148RΔEP153RΔCD2v (Group A - eight pigs) and BeninΔDP148RΔEP153R- CD2vE99R (Group B - six pigs).

Pigs were boosted on day 21 (10 ⁴ TCID50/ml) and day 28 (10 ⁶ TCID50/ml) post- immunisation and then challenged with 10 ³ TCID50/ml parental virulent Benin 97/1 strain on day 45 post-immunisation, in parallel with three non-immunised pigs (control Group E).

The experimental protocol is depicted in Figure 9.

Temperature

Rectal temperatures were recorded daily for all pigs in Group A and B and the control Group E (Figure 10). In Group A, no increase in temperature was observed before challenge. After challenge an increase in temperature at or above 41 °C was observed in one pig at day 3 post-challenge and in the remaining pigs at day 4 post-challenge. Two pigs (A3 and A4) reached the humane end-point at day 5 post-challenge and were culled. The remaining pigs had an increased temperature above 40.5 °C for two days in total, except for pig A7 in which a temperature of 40.6 °C persisted for a 3 ^rd day. All then survived to the end of the experiment at day 20 post-challenge without further increase in temperature (Figure 10A).

Three pigs in Group B had an increase in temperature above 40.5 °C at day 5 post- immunisation. One pig had a temperature of 41.1 °C by day 3 post-challenge and this was maintained a further 2 days when the animal was culled at the humane endpoint. From the other pigs all survived, one had a single day temperature above 40.5 °C at day 4 post- challenge, three had 2 days increase temperature (days 4 and 5) and one had no increased temperature. All survivors had no further temperature increase until the end of the experiment (day 20 post-challenge) (Figure 10B).

Control pigs had temperatures above 40.5 °C from day 4 post-challenge and were culled at the humane endpoint on day 6 post-challenge (Figure 10C).

Clinical scores

Clinical scores, including increased temperature and other signs such as lethargy and loss of appetite were collected daily (Figure 11). In Group A no signs were observed before challenge. After challenge clinical scores were observed in all pigs coincident with increased temperatures and reaching higher levels in the two pigs that were culled at the humane endpoint (Figure 11 A).

In Group B mild signs were observed in pigs post-immunization coincident with temperature increases. After challenge mild signs were observed except for the pig which was culled at the humane endpoint and the pig which did not show an increase in temperature which had no clinical signs (Figure 11B).

Viremia

Levels of virus genome in blood were measured by qPCR (Figure 12). No virus genome was detected in blood from pigs in Group A before challenge. After challenge variable levels of genome were detected from day 3 or 4 post-challenge. Maximum levels were detected in the pigs which were culled (~10e6 genome copies per ml). In other pigs the maximum levels detected varied between ~10e2 per ml and 10e5.5 per ml. Levels of virus genome decreased until termination at day 20 post-challenge becoming undetectable in 2 pigs and reduced to 10e2.6 in others except 1 pig in which levels remained at 10e4.5 at termination (Figure 12A).

In group B low levels of virus genome were detected in blood from 3 pigs at day 6 post- immunization. After challenge virus genome was detected at low levels (below 10e4 genome copies per ml) from day 7 post-challenge (Figure 12B).

Antibody response

ASFV specific antibody responses were measured using a commercially available competitive ELISA based on the VP72 (B646L) major capsid protein (Figure 13). In Group A antibody responses were first detected at day 27 post-immunization after the first boost and just before the second boost. Levels were maintained for the rest of the experiment (Figure 13A).

In pigs in Group B antibody responses were detected by day 14 (1 week before the boost) and increased maintaining a plateau after day 21 to 28 (Figure 13B).

Cell-mediated immune response

Peripheral blood mononuclear cells were collected pre-immunization, boost and challenge from pigs in Groups A and B and stimulated with ASFV Benin virus. Numbers of IFN gamma producing cells were measured by Elispot assay (Figure 14). In Group A low levels of IFN gamma producing cells were induced after immunisation and before boost and these increased uniformly in all pigs before challenge (Figure 14A). In Group B responses were more variable between pigs and pigs had had higher numbers of IFN gamma producing cells before boost. However at the time of challenge these numbers had decreased slightly in all except 1 pig. The pig which was culled at the humane end point (B4) had a lower number of IFN gamma producing cells although similar in number to a pig which survive challenge (B5) (Figure 14B).

Scoring of macroscopic lesions at post-mortem

Control non-immune pigs (Group E) showed lesions typical of acute ASFV including enlarged and haemmorhagic lymph nodes and spleen. Those pigs which survived challenge had few, if any, ASFV specific lesions (Figure 15). The pigs culled at humane end points showed higher scores but less than the control pigs (data not shown).

Viremia comparison

Figure 16 directly compares virus genome in blood after immunisation with different viruses. Immunisation with BeninΔDP148R induces moderate levels of virus genome transiently, which decrease slowly over 60 days. No increase in replication or detectable challenge virus is observed (Figure 16A). Deletion of the EP402R/CD2v gene (BeninΔDP148RΔCD2v virus) dramatically reduces the persistence of genome in blood (~15 days maximum) and reduces the level in some pigs. No virus genome was detected post-challenge (Figure 16B). The triple deletion BeninΔDP148RΔEP153RΔCD2v induces no detectable genome in blood post- immunisation and moderate to low levels after challenge decreasing to low or undetectable levels by the end of the experiment (Figure 16C). The BeninΔDP148RΔEP153R-CD2vE99R virus induces a transient low level of virus genome in blood after immunisation and low levels after challenge (Figure 16D).

All publications mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described methods and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in virology, molecular biology or related fields are intended to be within the scope of the following claims.