The present invention relates to a composition comprising at least one fibronectin binding protein, and/or at least one a truncated fibronectin binding protein and/or at least one fibronectin binding peptide, all comprising at least one fibronectin binding domain; and at least one iscom matrix complex and/or liposome and/or at least one lipid and at least one saponin, whereby the at least one lipid and the at least one saponin may be in complex, solution or suspension.

Further, the invention also relates to use thereof for the preparation of a vaccine against a micro organism that comprises at least one fibronectin binding domain. It also regards a kit of parts comprising at least two compartments, wherein one compartment comprises at least one truncated fibronectin binding protein and/or a fibronectin binding peptide, that comprises at least one fibronectin binding domain, and another compartment comprises an instruction for use and/or an iscom matrix complex and/or an iscom complex and or a liposome. Further it relates to a method for vaccination of an animal.

Technical background

SA is a pathogen, which causes diseases in virtually all mammalian species. SA is an important pathogen in the bovine, ovine and caprine species causing suffering and very high economical losses in the dairy breeds including cattle, sheep and goats {cattle) all over the world. The SA induced mastitis might be the single most impor- tant economical negative factor in veterinary medicine due to the pathogenicity including severe acute and painful inflammation, chronic and persistent conditions and the difficulties with efficient treatments mostly based on antibiotics that might not work because of the resistance leading culling. The alternative would be vaccination, but no efficient vaccine so far exists, which can constitute the basis for com- bating the mastitis problem caused by SA

There are several pathogenicity factors for a prospective protective vaccine against SA infections to overcome. E.g. SA "hides" antigens in the cell essential for the infection and for its survival. Further SA has the capacity to manipulate the host im- mune system to facilitate the bacterial existents. This complex situation is one reason that an efficient SA vaccine for protection against mastitis is not available.

SA components that manipulate and may down regulate the immune response of the host that includes excreted products such as α- and β-toxins, leukocidin. Also cell bound components like super antigens and protein A is involved.

Examples of essential structures that the bacteria hide for the immune system of the host it infects is Fibronectin binding proteins (FnBp) or external fibrin factor (Ebf) essential for adherence of SA to tissue e.g. in wounds.

Several Fibronectin binding microbial surface components recognizing adhesive matrixmolecules have been isolated and characterized from different Gram-positive bacteria. Genes encoding Fibronectin binding microbial surface components recognizing adhesive matrixmolecules from Staphylococcus aureus (Signas et al., "Nu- cleoside sequence of the gene for a fibronectin binding protein from Staphylococcus aureus: Use of this peptide sequence in synthesis of biologically active peptides," FrocrNatT. Acad ^" . Sci. USA. 86:699-703, 19890, Streptococcus pyogenes (Talay et al., "Fibronectin-binding protein of Streptococcus pyogenes: Sequence of the binding domain involved in adherence of streptococci to epithelial cells," Infect. Im- mun, 60:3837-3844, 1992.; Hansky et al. Infect. Immun., 60:5119-5125, 1992) and Streptococcus dysgalactiae (Lindgren et al, "Two different genes coding for fi- bronectin-binding proteins from Streptococcus dysgalactiae~the complete nucleotide sequences and characterization of the binding domains," Eur. J. Biochem, 214:819-827, 1993.) have been cloned and sequenced. The deduced amino acid se- quences revealed 60- 100 kDa proteins with very similar structural organization. The

N-terminal signal sequence is followed by a long stretch of unique sequence, which in some cases is interrupted by two copies of an approximately 30 amino acid long segment. The ligand binding site is located just N-terminal of a proline-rich domain, which is believed to anchor the proteins in the cell wall. This domain is followed by the sequence LPXTGX which is a cell wall targeting signal (Schneewind et al., Science, 268:103-106, 1995. et al., 1995), a stretch of hydrophobic residues representing a trans-membrane unit and a short C-terminal cytoplasmic domain containing a cluster of positively charged residues. Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus, have similar fibronectin binding de- vices and are of special interest for mastitis in the bovine and ovine species. Also coagulase negative Staphylococci have FnBp and are causing mastitis. The primary Fibronectin binding sites on these microbial surface components recognizing adhesive matrix molecules consist of 30-42 amino acid long motifs repeated 3-4 times, and most of the repeated units contain a consensus sequence (Lindgren et al., 1993 loc cit ; McGavin et al., "Fibronectin receptors from Streptococcus dysgalactiae and Staphylococcus aureus: Involvement of conserved residues in ligand binding," J. Biol. Chem. 268:23946-23953, 1993.). This domain is composed of a unit of 37-40 amino acids, repeated three or four times (FIG. 1 of USP 6,685,943).

Thus, FnBp is an important adhesion protein {antigen) for the formulation a protective vaccine against several gram-positive bacteria including SA based on the function of adhesion and even more as a target for phagocytosis being the main protective mechanism against SA. Importantly, FnBp is present in many isolates from gram-positive bacteria such as Streptococcus pyogenes and/or Streptococcus dysga- lactiae and Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus, and coagulase negative Staphylococcus. Close to 100% of SA isolates. A vaccine would not only be for protection of cows against mastitis caused by SA infection, but also for virtually all animal species affected by infections caused by SA including man.

The composition of vaccine antigens might vary in vaccines against gram-positive bacteria, including SA infections and SA caused disease depending on several factors including local strains and clinical pictures. The adhesion to fibronectin mediates an important and common factor for the infection process, and this protein is present on virtually all SA isolates. The blocking of adhesion and neutralisation is an effect by IgGl by antibodies, while IgG2a is important for phagocytosis the major immune protective mechanism against SA

In view of the fact that SA also is an intracellular parasite the cell mediated arm of the immune system (CMI) is an essential factor. It is well documented that the iscom system potently enhances CMI and in particular the cytotoxic T cells killing infected cells e.g. SA infected cells. Thus, the adjuvant formulations based on the iscom- technology have the capacity to evoke several immune protective mechanisms.

Iscoms containing FnBp have been reported {Nicker son Nelson et al.2000 (Symposium, Stresa Italy, Proceedings, 426-4319). A FnBp was incorporated into the iscom matrix to form an iscom with integrated antigen. Nelson's iscom formulation was however not sufficiently immunogenic and not useful for vaccines partly because of the short duration of immunity it induced. The present invention based on iscom ma- trixor liposomes as adjuvant gives a far better immunity (see present example 5 for comparison). Further, the vaccine formulation according to this invention is much better suited for large-scale production, since the iscom technology used in this invention only requires the addition of iscom matrix to the vaccine antigen suspension.

It has now turned out that the iscom technology is very well suited for preparation of vaccine compositions against gram-positive bacteria, including Staphylococcus aureus SA and especially against mastitis in the bovine species.

Summary of the invention

The present invention relates to a composition comprising at least one fibronectin binding protein, and/or at least one a truncated fibronectin binding protein and/or at least one fibronectin binding peptide, all comprising at least one fibronectin binding domain; and at least one iscom matrix complex and/or liposome and/or at least one lipid and at least one saponin, whereby the at least one lipid and the at least one saponin may be in complex, solution or suspension.

Further, it relates to the use thereof for the preparation of a vaccine against a micro organism that comprises at least one fibronectin binding domain. It also regards a kit of parts comprising at least two compartments, wherein one compartment comprises at least one truncated fibronectin binding protein and/or a fibronectin binding peptide, that comprises at least one fibronectin binding domain, and another compart- ment comprises an instruction for use and/or an iscom matrix complex and/or an iscom complex and or a liposome. Further it relates to a method for vaccination of an individual.

The invention is elucidated by way of the following tables and figures:

Table 1 :1

Lay out of the immunization of Balb/C mice with fibronectin binding protein

(FnBp). The mice were immunized twice s.c. four weeks apart with the respective vaccine candidates

Table 2:1

Lay out of the immunization of Balb/C mice tetanus toxiod (TT). The mice were immunized twice s.c four weeks apart with the respective vaccine candidates

Table 3:1

Lay out of the immunization of Balb/C mice with difteri toxiod (DT). The mice were immunized twice s.c. four weeks apart with the respective vaccine candidates

Table 3:1

Lay out of the immunization of Balb/C mice with difteri toxiod (DT). The mice were immunized twice s.c. four weeks apart with the respective vaccine candidates

Fig 1 :1 Serum antibody responses in IgGl and IgG2 subclasses to Fibronectin binding protein (FnBp) after the second immunization of Balb/C mice with FnBp in various adjuvant formulations

Fig 2:1 Serum antibody responses to Tetanus Toxoid (TT) after the second immunization of Balb/C with TT in various adjuvant formulations measured in IgGl and IgG2 subclasses.

Fig 3:1 Serum antibody responses in IgGl and IgG2 subclasses to Diptheria Toxoid (DT) after the second immunization of Balb/C with DT in various adjuvant formulations

Figs 4:1 through 4:4

Ten heifers were primed with FnBp adjuvanted with iscom matrix before going into lactation by intra vaginal (ivag) route (4:1) 1 animal out of 3 is shown; or by intranasal (i.n.) priming (4:2) 1 animal out of 3 is shown, or by s.c. administration (4:3) 1 animal out of 4 is shown. The second immunisation was for all groups carried out s.c. three to four weeks later and after that the lactation period had commenced. The antibody responses were analysed in serum and milk whey by ELISA by measuring the total total Ig, IgGl , IgG2 and IgA as depicted in the figures.

Figure 5:1

The antibody responses to fibronectin binding protein (FnBp) of five calves 4 to 7 months old vaccinated with FnBp adjuvanted with iscom matrix twice 6 weeks apart. The immune responses after the first immunization are recorded in the IgM and total IgG classes. After the second immunization the antibody responses are recorded in the IgGl and IgG2 subclasses.

Figure 5:2

The antibody responses to α (A) and β (B) toxins of 4 to 7 months old calves vaccinated twice six weeks apart with various formulations (see below). The antibody re- sponses were measured by toxin neutralization tests.

Five calves in each group were immunized with:

Group A: FnBp adjuvanted with iscom matrix Group B: FnBp plus Stphylococcus aureus (SA) and α and β toxins and adjuvanted with iscom matrix at the first immunization thereafter with FnBp only

Group C: FnBp with SA and plus α and β toxins and adjuvanted withiscom matrix at both immunizations Group D: SA and α and β toxins and adjuvanted with iscom matrix at both immunizations Group E: Non-immunized controls

Figure 6

The antibody responses of calves in the age of 1 to 2 years old vaccinated ones with fibronectin binding protein (FnBp) supplemented with Staphyloccocus aurus (A) cells or α and β toxins (B). The experimental vaccines were adjuvanted with iscom matrix. The antibody responses were measured in the IgM ₅ total IgG ₅ IgGl and IgG2 classes and subclasses.

Detailed description of the invention

The invention relates to a composition comprising at least one fibronectin binding protein (FnBp), and/or at least one a truncated fibronectin binding protein and/or at least one fibronectin binding peptide that comprises at least one fibronectin binding domain, and at least one iscom matrix complex and/or liposome and and/or at least one lipid and at least one saponin, whereby the at least one lipid and the at least one saponin may be in complex, solution or suspension.

The composition may be used for preparing vaccine against gram-positive bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Streptococcus dysgalac- tiae Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus.

A full length fibronectin binding protein may be used as well as a truncated protein or peptide as long as it comprises at least one fibronectin binding domain or epitope. By truncated protein we understand a naturally occurring or synthetically produced full length protein from which one or more amino acids have been deleted. One ore more of the amino acids in the non FnBp binding regions may be deleted. Amino acids from the FnBp binding may also be deleted. However, at least one FnBp bind- ing domajn or epitope should

Preferably the truncated protein or peptide comprise one ore fibronectin binding domains from a gram-positive bacteria, especially from Staphylococcus aureus,

Streptococcus pyogenes, Streptococcus dysgalactiae Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus

The fibronection binding domain may be selected from the D domains from fi- bronectin binding protein A or B from Staphylococcus aureus, such as the DU and

D1-D4 domains; the A domains from fibronectin binding protein A from Streptococcus dysgalactiae, such as the AU and A1-A3 domains; the B domains from fibronectin binding protein B from Streptococcus dysgalactiae, such as the B1-B3 domains; and the domains from fibronectin binding protein Streptococcus pyogenes such as the P1-P4 domains (see fig. 1 of USP 6,685,943). FnBp binding domains of Staphylococcus aureus are also described in Signas et al.: (1989) Nucleotide sequence of the gene fibronectin-binding protein from Staphylococcus aureus: Use of this peptide sequence in the synthesis of biologically active peptides. Proc. Natl Acad. Sci. USA, Vol:86 pp. 699 - 703.

The truncated protein or peptide may comprise 1-200, preferably 1-100 such as 1- 50, such as 1-20 amino acids up stream or down stream of one or more of the FnBp binding domains e.g. the D, A, P domains mentioned above.

The truncated protein and the peptide may be a naturally occurring amino acid sequence or be synthetically produced, or produced by hybrid DNA technology. One ore more of these FnBp binding domains or several repeating units comprising one or more of the above mentioned FnBp binding domains may be produced and used according to the invention.

How to obtain naturally occurring and synthetically produced FnBp binding domains are generally described in USP 6,685,943

The identification of Fn-binding domains or epitopes is generally known in the art. For example, one may employ the methods of Hopp, as taught in U.S. Pat. No.

4,554,101, which teaches the identification and preparation of epitopes from amino acid sequences on the basis of hydrophilicity. The methods described in several other papers, and software programs based thereon, can also be used to identify epi- topic core sequences (see, for example, Jameson and Wolf, 1988; Wolf et al., 1988; U.S. Pat. No. 4,554,101). The amino acid sequence of these "epitopic core se-

quences" may then be readily incorporated into peptides, either through the application of peptide synthesis or recombinant technology.

In general, the size of the domain or epitope is not particularly crucial, so long as it is at least large enough to bind to FnBp. The smallest useful core sequence expected by the present disclosure is generally on the order of about at least 5 amino acids in length, with sequences on the order of 10 or 50 being more preferred. One or more FnBp domains or epitopes may be used.

The identification of epitopic core sequences is known to those of skill in the art, for example, as described in U.S. Pat. No. 4,554,101 which teaches the identification and preparation of epitopes from amino acid sequences on the basis of hydrophilic- ity. Moreover, numerous computer programs are available for use in predicting antigenic portions of proteins (see e.g., Jameson and WoIf, 1988; Wolf et al., 1988). Computerized peptide sequence analysis programs (e.g, DNAStar 7 software,

DNAStar, Inc., Madison, Wis.) may also be useful in designing synthetic FnBps, and FnBp-derived epitopes and epitope analogs in accordance with the present disclosure.

In this regard, particular advantages may be realized through the preparation of synthetic peptides that include epitopic/immunogenic-core sequences. These epitopic core sequences may be identified as hydrophilic and/or mobile regions of the polypeptides or those that include a T cell motif.

To confirm that a protein or peptide is immunologically cross-reactive with, or a biological functional equivalent of, one or more epitopes of the disclosed peptides is also a straightforward matter. This can be readily determined using specific assays, e.g., of a single proposed epitopic sequence, or using more general screens, e.g., of a pool of randomly generated synthetic peptides or protein fragments. The screening assays may be employed to identify either equivalent antigens or cross-reactive anti-

bodies. In any event, the principle is the same, i.e., based upon competition for binding sites between antibodies and antigens.

Suitable competition assays that may be employed include protocols based upon immunohistochemical assays, ELISAs, RIAs, Western or dot blotting and the like. In any of the competitive assays, one of the binding components, generally the known element, such as the FnBp-derived peptide, or a known antibody, will be labelled with a detectable label and the test components, that generally remain unlabeled, will be tested for their ability to reduce the amount of label that is bound to the corresponding reactive antibody or antigen.

Useful peptides for use according to the invention may be obtained as described in the examples, general information before example 1 and in example 1.

In order to better purify the truncated protein or peptide these may be produced by DNA engineering and a sequence inserted coding for an amino acid sequence having affinity for materials that may be used for separation. For example one may introduce 6 histidine; one or more positive amino acids; and/or the zz(z) region from protein A that bind to a chelating (Ni - or Co-) material; an ion exchange material and immuno affinity material respectively. Such methods are generally known in the art.

The fibronectin binding protein, the truncated fibronectin binding protein and the fibronectin binding peptide that comprises at least one fibronectin binding domain may be isolated from the bacterial cell of any of the above mentioned bacteria. The may also be isolated from excreted products from the bacteria.

Further the antigens may be produced as rDNA products from bacterial cells; fungal or from mammalian cells according to known technology.

They may also be produced in vector systems; or in vivo after immunization with so called DNA vaccines or be produced as so called RNA vaccines as is generally known in the art.

They may also be produced as fusion proteins and be e.g. rDNA products from bacterial cells; be produced as fusion proteins and be rDNA products from fungal cells; be produced as fusion proteins and be rDNA products from mammalian cells; or produced as fusion proteins in vector systems from bacterial cells; produced as fusion proteins in vector systems from fungal cells; produced as fusion proteins in vector systems from mammalian cells.

Further, the fusion product may be a conjugate between a protein and a carbohydrate capsular antigen from a gram-positive bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Streptococcus dysgalactiae Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus, or the fusion protein product may be a conjugate between two proteins where at least one of the proteins is a bacterial protein from any of the above mentioned micro organisms such as a Staphylococcus aureus protein.

The fibronectin binding protein and/or the truncated fibronectin binding protein and/or fibronectin binding peptide may be integrated into liposomes or mixed with iscOnrmatrixoτ-liposσmes"or wαpied ^' on liposomes Orisϋom matrix

The iscom matrix complex in the compositions of the invention comprises at least one glycoside and at least one lipid. The lipid is at least a sterol such as cholesterol and optionally also phosphatidyl choline, matrixhas an immunoenhancing effect on co-administered antigenic substances, see EP 0 436 620 Bl and may be produced as described in this patent.

The composition iscom, the matrix complexes and/or the liposomes, may also contain one or more other immunomodulatory (adjuvant-active) substances, not necessarily a saponin, e.g. lipopolysacharides (LPS), Lipid A or Lipid A derivatives, CT or LT and their sub-fragments or derivatives thereof e.g., LTB, LTA, CTB, CTA or CTAl-DD

The iscom matrix may be one or more iscom matrix particles or any sub-fragment(s) of the 6 nanometre rings thereof. Any mixtures of such iscom matrix, particles or sub fragments may be used. One or more antigens may be used and a transport and passenger antigen may be used as described in EP 9600647-3 (PCT/SE97/00289).

The lipids used are particularly those described in the applicant's patent EP 0 109 942 Bl in particular on p. 3 and in patent EP 0 436 620 Bl on p. 7 lines 7-24. Especially sterols such as cholesterol and phospholipids such as phosphatidylethanolamin and phosphatidylcolin are used. Lipid-containing receptors that bind to the cell- binding components, such as glycolipids including the cholera toxin's receptor, which is the ganglioside GMl, and fucosed blood group antigen may be used. The cell-binding components can then function as mucus targeting molecule and be bound to the lipid-containing substances through simply mixing them with com- plexes that contain them. Iscom complexes comprising such receptors and receptors are described in WO 97/30728.

Useful glycosides are described in EP 0 109 924 Bl. Saponins and triterpensaponins are preferred. The may be in the form of raw extract from Quillaja Saponaria Molina" (Dalsgaard, K. (1974), Arch. Gesamte Virusforsch, 44, 243.), or any sub- fraction thereof as described in PCT/US/88101842 to Kensil et al., Kensil, CA. et al. (1991), J. Immunol, 146, 431, Kersten, G.F.A. et al. (1990). "Aspects of Iscoms. Analytical, Pharmaceutical and Adjuvant Properties; Thesis, University of Utrecht, EP 0 362 279 B2 and EP 0 555 276 Bl.

According to one aspect of the invention the iscom matrix complex comprises crude or raw extract of Quil A comprising a mixture of saponines or a seimipurified form thereof such as Quillaja Powder Extract (Berghausen, USA), Quillaja Ultra Powder QP UF 300, Quillaja Ultra Powder QP UF 1000 or Vax-Sap (all three from Natural Responses, Chile).

According to another aspect of the invention the iscom matrix complex comprises at least one purified glycoside such as a saponin fraction from Quil A.

The purified saponin fractions according to the invention may be the A, B and C fractions described in WO 96/11711, the B3, B4 and B4b fractions described in EP 0 436 620 The fractions QA1-22 described in EP 0 3632 279 B2, Q-VAC (Nor- Feed, AS Denmark), Quillaja Saponaria Molina Spikoside (Isconova AB, Uppsala Science Park, 751 83, Uppsala, Sweden).

The fractions QA- 1-2-3-4-5-6-7-8-9- 10-11-12-13-14-15-16-17-18-19-20-21 and 22 of EP 0 3632 279 B2, Especially QA-7, 17-18 and 21 may be used. They are obtained as described in EP 0 3632 279 B2, especially at page 6 and in Example 1 on page 8 and 9. Preferably sub fractions A and C are used.

The term "one saponin fraction from Quillaja Saponaria Molina." is used throughout this specification and UTtKeT claims as a generic description of a purified of ^" defined saponin fraction of Quillaja Saponaria or a substantially pure fraction. It is important that the fraction does not contain as much of any other fraction to nega- tively affect the good results that are obtained when the mixtures of iscom or iscom matrix comprising essentially one fraction is used. The saponin preparation may, if desired, include minor amounts for example up to 40% by weight, such as up to 30 % by weight, up to 25 % by weight, up to 20 % by weight, up to 15 % by weight, up to 10 % by weight, up to 7 % by weight, up to 5 % by weight, up to 2 % by weight,

up to 1 % by weight, up to 0,5 % by weight up to 0,1 % by weight of other compounds such as other saponins or other adjuvant materials.

The composition according to the invention may comprise a mixture of at least two iscom complexes, chosen from iscom matrix complexes, each complex comprising essentially one different saponin fraction from Quillaja Saponaria Molina as described in WO 2004/004762 (PCT/SE03/01180).

Preferably mixtures of matrixare used in which the fraction Quillaja Saponaria Molina and fraction Quil C are separately incorporated into different iscom complexes or matrix. As mentioned above any combinations of weight % of the different iscom complexes based on their content of fraction A and C of Quillaja Saponaria Molina respectively may be used. The mixtures may comprise from, 0,1 to 99,9 by weight, 5 to 95% by weight, 10 to 90% by weight 15 to 85% by weight, 20 to 80% by weight, 25 to 75% by weight, 30 to 70% by weight, 35 to 65% by weight, 40 to 60% by weight, 45 to 55% by weight, 40 to 60%, by weight, 50 to 50% by weight, 55 to 45% by weight, 60 to 40% by weight, 65 to 35% by weight, 70 to 30% by weight, 75 to 25% by weight, 80 to 20% by weight, 85 to 15% by weight, 90 to 10% by weight, 95 to 05% by weight, of iscom matrix complexes comprising fraction A of Quillaja Saponaria Molina (as herein defined) and the rest up to 100 % in each case of interval of iscom matrix complexes comprising fraction C of QnUiaja SaponarraMoimE (as herein defined), counted σnlhe content of the sum fractions A and C of Quillaja Saponaria Molina in the iscom matrix complexes.

The mixture may comprise from 75% to 99,5% by weight of fraction A and 0,5% to 25% by weight of fraction C. Preferably, the mixture comprises from 90% to 99% by weight of fraction A and 1% to 10% by weight of fraction C. A particularly preferred preparation comprises about 91% to 98% by weight of fraction A and about 2% to 9% by weight of fraction C, especially about 92% to 96% by weight of frac-

tion A and about 4% to 8% by weight of complexes of fraction C counted on the content of the sum fractions A and C of Quillaj ^' a Saponaria Molina in the iscom complexes.

All intervals mentioned above may be used for any combination of any fraction of Quillqj a Saponaria Molina in formulations for administration to any type of human or animal species. Examples of animal species to which the formulations according to the invention may be administrated are companion animals such as cats, dogs, horses, birds such as parrots, economical important species such as cattle, e.g. bo- vine species, swines, sheep, goats. Preferably more than 50% by weight of fraction C is used in combination with any of the other fractions and especially in combination with fraction A. Thus, from 50.5 - 99.5% by weight of C and 0,5 - 49,5% by weight of A may be used.

According to one embodiment of the invention the iscom matrix complex comprise fraction A of Quil A together with at least one other adjuvant as described in WO 2005/002620 (PCT/SE2004/001038). Such iscom complex and Iscom matrix complex may comprises 50-99,9% of fragment A of Quil A and 0,1-50% of fragment C and/or fraction B and/or other fractions or derivatives of Quil A counted on the total weight of fractions A and C, wherein the different glycoside components may be integrated into, coupled on to or mixed with the same or different complex or iscom matrix-particles.

The truncated fibronectin binding protein and/or a fibronectin binding peptide, that comprises at least one fibronectin binding domain, may also be integrated into, coupled to or mixed with liposomes. Liposomes can be produced as described in Grego- riadis, G., McCormack, Obrenovic, M., Perrie, Y. and Saffie, R. In Vaccine Adjuvants, Preparation Methods and Reseach Protocols. (2000) Ed. O'Hagan D.,ρp 137- 150. Liposomes as immunological adjuvants and vaccine carriers.

They may also be integrated into, coupled to or mixed with and/or at least one lipid and at least one saponin, whereby the at least one lipid and the at least one saponin may be in complex, solution or suspension. Preferably the complex here is not in the iscom form.

The fibronectin binding protein, the truncated fibronectin binding protein and the fibronectin binding peptide that comprises at least one fibronectin binding domain may be considered as antigens. The composition according to the invention may comprise at least one further antigen.

According to one embodiment of the invention the at least one further antigen are antigens in the form of whole cells of gram-positive bacteria.

In another embodiment of the invention the antigens are antigenic components such as from the bacterial cell or excreted products such as α and β haemolysins especially from gram-positive micro organisms.

The gram-positive bacteria from which these whole cells and antigenic components are obtained may be Staphylococcus aureus, Streptococcus pyogenes, Streptococ- cus dysgalactiae Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus, and coagulase negative Staphylococcus. By Staphylococcus ^" SSP ^g s∑TCells lB ^' meanTthe ^^Qv^^δύp ^' ^^M ^' ΕfάphytbΥoTcl^^^^.T^nQ ¹ spondingly Streptococcus pyogenes, Streptococcus dysgalactiae Streptococcus agalactiae and Streptococcus uberis and coagulase negative Staphylococcus, and coagulase negative Staphylococcus refer to the respective group of the genus. This also applies to the micro organisms from which the FnBp binding protein, peptide or domain are obtained.

Adhesins may be chosen from clumping factor (elf), external fibrin binding protein (efb), A and B Adhesins to fibrinogen, Coagulase (coa), fibrogen binding protein A

is binding to fibrinogen Firbronectin binding protein (FnBp) A and B; attachment to fibrinogen, collagen binding protein (cna); binding to collagen, Elastin binding protein (ebpS); binding to elastin, MHC analogous protein (map or eap); binding to extra cellular matrixproteins, Polysaccharide intracellular adhesin (pia); intracellular adhesion and biofilm formation, Protein A (spa); possible evaison of host defence, Capsular polyscharides (e.g. types 1, 5, 8 and 13)(cap); antiphagocytic molecules or Techoid acid.

Pore forming factors, may be excreted and may be chosen from alpha-haemolysin, beta-haemolysin, gamma-haemolysin, delta-haemolysin, Phopholipase C(plc; lysis of host cell, Elastase (sepA) tissue invasion, and Hyaluronidase (hysA) tissue invasion.

AU mentioned components may be proteins or carbohydrates isolated from the bacterial cells, excreted products, rDNA products or fusion products expressed as rDNA products, products or fusion proteins in vector systems, so called DNA or RNA vaccines as long they use the present adjuvant system.

According to sill another embodiment of the invention the antigens may be antigenic components that down regulate the immune system such as super antigens, capsular antigens, endotoxins, exotoxins and extra cellular enzymes.

Sπch ^' exσto^mFaTid ^' exGfa ^' Cellurεirenzymes may be cRosehlfόfn enterotoxins A toΕ, ^' H (sea-e, h), Toxin shock syndrome toxin- 1 (tst); evasion of host defence with super antigen, properties, exfoliation toxins AB (eta, efb. etb, evas of host defences, Li- pase (geh); evasion of host defence, Panton-Vallentine leukociddin (lukF, lukS), lysis of host phagocytes, evasion of host defence, Staphylokinase (sak) evasion of host defence.

The antigenic components mentioned above may differ between species from the groups of the genus from different regions of the world.

The information above about the origin of the truncated fibronectin binding protein and/or a fibronectin binding peptide is also applicable to the at least further antigen that the composition may comprise.

The compositions according to the invention may further comprise a pharmaceutically acceptable carrier, diluents, excipient or additive.

The pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents, which have been mentioned above. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono-or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

The solutions or suspensions could also comprise at least one of the following adjuvants fstefile diluents sucrTas waterfoTinjectiόn, ^" saline, ^" fixed ^~ oils7 polyethyleiϊe glycols, glycerol, propylene glycol or other synthetic solvents, antibacterial agents such as benzyl alcohol or methyl paraben, antioxidants such as ascorbic acid or so- dium bisulfite, chelating agents such as ethylene diamine tetraacetic acid, buffers such as acetates, citrates or phosphates, and agents for adjustment of the tonicity such as sodium chloride or dextrose. The parenteral preparation could be enclosed in ampoules, disposable syringes or multiple dosage vessels made of glass or plastic. The compounds of general formula I may be administered parenterally. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscu-

lar, intradermal injection of infusion techniques, for needle less injection -jet injection.

The composition may be in form of a vaccine composition for jet injector compris- ing some hundred doses for the vaccination of e.g. 200 cows.

The Composition according to the invention may further comprise an antigen composition affecting the udder such as coagulase negative Staphylococci, Streptococcus uberis, Streptococcus dysgalacti, Streptococcus agalacti, Coliforma bacteria in- eluding Klebsiella sp.p, and E. coli.

The invention also relates to the use of the compositions for preparing vaccines. The vaccines are intended for any mammal such human beings, animals such as cattle, sheep or goat. The invention especially relates to the vaccination of bovine, ovine and caprine animals against mastit. The invention especially relates to a mastit vaccine for bovines comprising an antigen from against Staphylococcus aureus mixed with iscom matrix.The matrix is preferably produced from crude Quil A or semi purified Quil A.

The bovines may be vaccinated once before calving and a booster before or after calving, {preferably after) 1 month. Heifers may be given to two administrations be- -fars-cΦήπg,j>refeτabiy possibly followed by a ^~ thira"admin ^* isTratiori after ^" calving:

One embodiment of the invention relates to a composition according to claim 1 comprising at least one further antigen chosen from toxins and/or whole cells from a micro organisms especially from gram-positive bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Streptococcus dysgalactiae Streptococcus agalac- tiae and Streptococcus uberis and coagulase negative Staphylococcus, especially form Staphylococcus aureus. Thus the composition may comprise toxins or whole cells or both.

It has turned out that for heifers above about one year of age better immunisation is obtained when a composition according to the invention is used supplemented with whole cells and/or toxins from gram-positive bacteria, especially from the ones mentioned above such as Staphylococcus aureus.

The invention further relates to kit of parts wherein one compartment comprises at least one fibronectin binding protein and/or at least one truncated fibronectin binding protein and/or at least one fibronectin binding peptide, that comprises at least one fibronectin binding domain, and another compartment comprises an instruction for use and/or at least one iscom matrix complex and/or a liposome.

Gram-positive micro organism species mentioned above, such as Staphylococcus aureus species from the aureus group of the Staphylococcus genus, may differ from different regions of the world. One compartment of the kit may comprise an iscom matrix complex or liposome according to the invention. This compartment may comprise one or more antigens from a gram-positive micro organism e.g. the aureus group of the Staphylococcus genus. The other compartment may comprise an anti- gen from a gram-positive micro organism, e.g. form the aureus group of the Staphylococcus genus that may be specific for a certain region e.g. based on capsular anti- iscom matrix complex or mixed with iscom matrix complex or liposomes.

As the gram-positive micro organism species mentioned above, such as Staphylococcus aureus species from the aureus group of the Staphylococcus genus, may elicit antigens that down regulate the immune system and antigens that does not down regulate the immune system it could be advantageous to administrate these different types of antigens in different formulations to different parts of the animal body to increase the effect of each components following the immunisation. Kits may there-

fore comprise at least one compartment comprising at least one antigen that down regulate the immune system and another compartment comprising at least one antigen does not down regulate the immune system.

The composition and the kit may also comprise an antibiotic. This may be useful when the animal has a sub clinical or clinical infection of the above mentioned micro-organisms.

The amount of antigenic substance_might vary, dependent on the substance and mi- cro-organisms used and the individual to be treated. For small animals the low dose is 0.1 μg up to 100 μg, for large animals the low dose range from 10 μg up to 1000 μg, especially 10 μg up to 300 μg that said not to be limiting borders. In humans the dose ranges are 1 μg up to 200 μg not being the limiting border.

The invention also relates to a method for vaccination of mammals such as human beings and especially catties, wherein a composition comprising at least one fi- bronectin binding protein, and/or at least one a truncated fibronectin binding protein and/or at least one fibronectin binding peptide, that comprises at least one fibronectin binding domain, and at least one iscom matrix complex and/or liposome is administrated to the animal.

Immunization protocol for a prospective mastitis vaccine may be as follows:

For heifers going into lactation two immunizations are carried out s.c. with 5 to 8 weeks interval. The last immunization is done round ten days before calving. After that it is recommended with a yearly immunization 10 to 14 days before expected calving.

In the case a cow that has a sub clinical or a clinical SA mastitis it will be recom- mended to vaccinate twice with 2 to 3 weeks interval during the dry period and in

that connection treat the cow with antibiotic i.e. a combined immunological and antibiotic treatment.

All information regarding the fibronectin binding protein, the truncated fibronectin binding protein and/or a fibronectin binding peptide, that comprises at least one fibronectin binding domain, the lipids, glycosides and other added antigens besides the fibronectin binding protein or peptide relate mutatis mutandis to all embodiments of the invention.

While the invention has been described in relation to certain disclosed embodiments, the skilled person may foresee other embodiments, variations, or combinations which are not specifically mentioned but are nonetheless within the scope of the appended claims.

All references cited herein are hereby incorporated by reference in their entirety.

The expression "comprising" as used herein should be understood to include, but not be limited to, the stated items.

The invention will now be further elucidated by way of the following non limiting examples.

Examples General information for examples Materials Adjuvants

A1(OH)3 - A classical adjuvant for use in animals and in humans. The adjuvant effect is still not fully understood. Antibody responses are enhanced compared with no

adjuvant but the response is strongly TH2 biased. No or low adjuvant effect on cellular responses. A1(OH)3, Allhydrogel was from Brenntag AG, Denmark.

Iscom matrix adjuvants

Matrix-Q - is produced from semi-purified Quillaja saponin containing a whole range of different Quillaja saponin molecules. The adjuvant effect is strong and includes both humoral and cellular immune responses. Matrix-C is produced from one purified fraction of Quillaja saponin (Fraction-C). It has a potent adjuvant activity (Johansson, M and Lovgren-Bengtsson, K Vaccine 17, 2894-2900). Toxicity is considerably lower than that of Matrix-Q. Matrix-QWT is produced from another purified fraction of Quillaja saponin (Fraction- A). The adjuvant effect is described as lower that for Matrix-C (PCT/SE2004/001038) but it appears to be strong for cellular immune responses. Matrix-QWT appears to be essentially non-toxic in doses relevant for adjuvat activity and is very well tolerated in all animal species tested.

Matrix-MIX - is a mixture of Matrix-QWT and Matrix-C (consisting of 17 % Matrix-C and 83 % Matrix-QWT). Similar mixtures were shown to exert surprisingly high adjuvant effect for single antigens (WO 2004/004762, PCT/SE2004/001038). All matrixadjuvant formulations were from Isconova AB, Uppsala, Sweden. Antigens

Tόrm ^' almlreaTed ^' Tetanus ^' toxόϊg TTTT wasTrom The ^" State ^' SerϋmTnsϋtute ^' , Copenhagen, Denmark. TT is a considerably immunogenic antigen. A higher dose (2,5 If) and a low dose (0,5 Lf) were used in the examples. For this particular batch, 1 Lf of toxoid corresponds to x micrograms of protein.

Recombinant Diptheria toxoid (DT) was from Sigma. A dose of 1 microgram was used in the examples.

Fibronectin binding protein (FnBp) was manufactured by Crosslink Ltd Budapest Hungary and was a mixture of FnBp A Short (396 bp with 100% conformity of

FnBp A of NC_002951.2) and FnBp B Short (396 bp with 95 % conformity of FnBp B of NC_002951.2 and from Biostapro AB Ulltunaallen 2B 756 51 Uppsala, Sweden and was a DNA fragment including a 16 kD fibronectin binding domain including 3 DD repeats including a cystein N terminalt not essential for the immuniza- tion result. 190 μg of this construct was mixed with 1 mg iscom matrixper dose.

ELISA

Mouse sera. Antigen specific antibodies in serum were measured in individual samples by conventional indirect ELISA. Microtiter plates (Nunc, Roskilde, Denmark) were coated over night at +4°C with the individual antigens at a concentration of 1 μg/ml in 5OmM carbonate buffer pH 9,6. Prior to incubation with samples, the plates were blocked for 1 hour at r.t. with PBS-T (Phosphate Buffered Saline with 0,05% Tween) supplemented with 2% (w/v) fat free milk powder (PBS-T/M). The plates were incubated sequentially with serial dilutions of test sera in PBS-T/M and HRP-conjugated rabbit anti-mouse IgG (Dakocytomation, Denmark). For measurement of IgG subclasses, serial dilutions of test sera were incubated with HRP- conjugated goat-anti-mouse IgGlHRP (Serotec, Norway) or with HRP-conjugated goat-anti-mouse IgG2a HRP (Serotec, Norway) . The enzyme reaction was visualised by incubation with substrate buffer (K-blue, SVANOVA, Upppsala, Sweden), the reaction was stopped after 10 minutes by addition of 50 μl 2 M H2SO4 and the absorbance was read at 450 nm. AU washings were done with PBS-T. Conjugates were dirated Ηc^ording to the^^ calculated by interpolation of the linear part of the titration curve and are expressed as reciprocals of the serum dilution giving an absorbance of 1,0.

Cow sera and milk. Antigen specific antibodies in serum and milk were measured in individual serum (milk) samples by conventional indirect ELISA. Microtiter plates (Nunc, Roskilde, Denmark) were coated over night at +4°C with FnBp at a concentration of 1 μg/ml in 5OmM carbonate buffer pH 9,6. Prior to incubation with sam- pies, the plates were blocked for 2 hour at r.t. with PBS-T (Phosphate Buffered Sa-

line with 0,05% Tween) supplemented with 10% (w/v) horse serum(PBS-T/H ). The plates were incubated sequentially with serial dilutions of test sera (milk) in PBS- T/H and HRP-conjugated sheep anti-bovine IgG (Serotec, Norway). For measurement of IgG subclasses, serial dilutions of test sera were incubated with HRP- conjugated sheep anti-bovine IgGl (Serotec, Norway) or with HRP-conjugated sheep anti-bovine IgG2 HRP (Serotec, Norway), with HRP-conjugated sheep anti- bovine IgA

(Serotec, Norway) The enzyme reaction was visualised by incubation with substrate buffer (K-blue, SVANOVA ₅ Upppsala, Sweden), the reaction was stopped after 10 minutes by addition of 50 μl 2 M H2SO4 and the absorbance was read at 450 nm. All washings were done with PBS-T. Conjugates were diluted according to the manufacturers instructions in PBS-T/H. Titres were calculated by interpolation of the linear part of the titration curve and are expressed as reciprocals of the serum dilution giving an absorbance of 1,0.

Antibodies against Staphylococcal bacteria were measured in serum and milk using a commercial ELISA (Staphylococcus Aureus Antibody Test Kit, WMRD, Pullman, WA, USA).

Example 1 Preparatin of fibronectin binding domains.

Cloning of Fibronectin Binding Protein

Project carried out by Report written by Ordered by

Crosslink Ltd. Ferenc Felfδldi, Prof. Bror Morein

Budapest, Hungary Crosslink Ltd. ISCONOVA,

Uppsala, Sweden

Content Introduction pi

A. Cloning of the full length version with 6-His tag at C terminal pi p3

B. Cloning of the short version with 6-His tail at the C terminal

Introduction

The aim of the project was to clone and express FNBP gene from Staphylococcus aureus a.) foil length version with 6-His tag at C terminal for protein preparation; b.) short version with 6-His tail at the C terminal for linking to the adjuvant;

The strain which cause mastitis in cows was provided by the ISCONOVA Ltd., Uppsala, Sweden. However, sequence information of this strain was not available.

A. Cloning of the full length version with 6-His tag at C terminal

In S. aureus two types of gene code for Fibronectin Binding Protein, i.e., FNBP A and FNBP B gen. We investigated the NCBI Data Bank (www.ncbi.nlm.nih.gov/blast) for FNBP and found sequences for eight S. aureus strains.

There was only 80% similarity of the eight strains in the FNBP genes. In order to be sure that the FNBP gene of our strains can be amplified on the foil length (excluding the signal sequence), we had to select primers which amplify both A and B genes.

Preparation of target DNA from Staphylococcus aureus. _

The genomic DNA was prepared from S. aureus suspension by traditional method and used in PCR reaction as template. Fortunately the length of the A and B gene is differs by 200 bp. Thus, in a long electrophoresis gel the separation of the two PCR products was possible. The A gene product was separated and used in the following steps.

1. Fifty ml S. aureus culture (OD600=2) were centrifoged and washed two times in 2x25 ml washing buffer (150 mM NaCl, 50 mM Tris HCl [pH: 7.5]).

2. The cells were resuspended in 5 ml lysis bufferA (10 mM EDTA, 50 mM Tris HCl [pH: 7.5], 0.1 mg/ml lyzozym) and kept on room temperature for 15 min.

3. Five ml lysis bufferB (2 % SDS, 50 mM Tris HCl [ρH:7.4], 0.025 mg/ml Proteinase K) was added. The suspension was incubated at 55oC for over night.

4. The suspension was extracted with one volume phenol followed with extraction with one volume chloroform + i-amylalcohol.

5. The DNA was precipitated with 2.5 volume ethanol and spined onto a glass rod. The DNA was washed with 70% ethanol and dried in Eppendorf tube.

6. The DNA was resuspended in 250 microliter TE buffer.

In the next step we digested both PCR products with different restriction enzymes in order to select those, which don't have cleavage site in the PCR products. They can ensure cloning at the ends without damaging the insert.

We found three restriction enzymes, BamHI, HindIII and Xhol that do not cleave into FNBP A. We decided to go on with FNBPA ₅ and the BamHI and HindIII to be used for cloning, while the Xhol for His tag addition to the C terminal.

The primers used for amplifications have the above cleavage sites at their 5 'end (bold text):

FnBpaF-

CGGGATCCTGCAGCATCAGAACAAAAGACAACTACAGT

FNBPaR GTAAGCTTATGCTTTGTGATTCTTTTTATTTCTGCGTAA

Amplification of the FNBP gene:

The full length of FNBP A gene from the desired strain was amplified, cloned and confirmed by sequencing.

In order to make later purification more efficient, the membrane binding domain (M) and the repetitive and non repetitive part of cell-wall spanning domain (Wl and W2) of the gene was removed.

For the domain descriptions see article Signas et al.: (1989 loc.cit.)

Ncol and the above mentioned Xhol restriction enzymes were proper for this aim.

The designed primers where:

FnBpNco

CGGGATCCATGGCATCAGAACAAAAGACAACTACAGT

(primer starts at pos. 2572388 OF >ref|NC 002951.2| Staphylococcus aureus subsp. aureus COL, complete genome Length=2809422)

FnBpD4

GAGCTCGAGTGGCACGATTGGAGGTGTTGTATCTTCT

(primer starts at pos. 2569863 OF >refjNC 002951.2| Staphylococcus aureus subsp. aureus COL, complete genome Length=2809422)

The following steps were used

1. One hundred microliter amplification solution contained 10 microliter IOXPCR buffer, 10 microliter of each primer (10 pmol/microliter stock), 400 micromol dNTP and 5 U Pfu enzyme.

2. The amplification was carried out in 30 cycles of denaturation (94oC for 45 s), anriealing.(65oCJbx 1 mύiXand-poIymejisώiorj_(72αCJor-2jnin _s ) _r

3. The PCR products were checked in agarose gel and purified by Single Sample PCR Cleanup Montage ^® PCR Filter Units (Millipore).

Cloning of the PCR product

The FNBP gene was cloned into pET21d vectors. A codon for 6-His tag, followed by a stop codon, is situated behind the Xhol cleavage site of the pET21d vector. As a consequence, the expressed protein will contain a 6-His tag at the C terminal.

The expression was carried out for 8 hours after induction. The cells were sonicated and centrifuged after expression. The products were tested on SDS-PAGE and commassie blue staining with or without preparation on Ni-NTA agarose column. The expression was clear compared to the non-induced culture.

The following steps were used

1. The PCR product was resuspended in 40 microliter NEB2 buffer and digested at 37oC for 2 hours by Ncol and Xhol restriction enzymes.

2. The digested fragment was purified from gel by Montage Gel Extraction Kit (Millipore).

3. The purified DNA was cloned into the pET21 d vectors at the Ncol/Xhol site in 50 microliter reaction solution. The vector and the insert were ligated over night by 11 U ligase enzyme.

4. The plasmid was precipitated with 2.5 vol. ethanol, resuspended, and used for electroporation (Bio-Rad) into E.coli BL21 strain according to the instruction of the supplyer. 5. The clones were cultured in LB media and the insertion of the FNBPA gene into the vector was checked by Ncol/Xhol digestion, followed by gel electrophoresis.

6. The appropriate vector was transformed into the E. coli BL21 strain again, grown in 20 times 30 ml parallel culture in LB media. The cells were induced with 1 mM IPTG at OD600=1.

B. Cloning of the short version with 6-His tail at the C terminal

The short version was cloned with the following primers. The cleavage sites of the Ncol restriction enzyme and the Xhol restriction enzyme indicated by bold letters.

BGF CGGGATCCATGGAAGGTGGCCAAAATAGCGGTAACCAGT

(primer starts at pos. 2570270 OF >ref1NC 002951.21 Staphylococcus aureus subsp. aureus COL, complete genome Length=2809422 )

BGR

GAGCTCGAGAGGTGTTGTATCTTCTTCAATCGTTTGTTG

(primer starts at pos. 2569875 OF >reflNC 002951.21 Staphylococcus aureus subsp. aureus COL, complete genome

Length=2809422 )

The PCR product made with these primers were cloned into the pET21d vector and expressed in BL21 (DE3) Star cells. A codon for 6-His tag, followed by a stop codon, is situated behind the Xhol cleavage site of the pET21d vector. As a consequence, the expressed protein will contain a 6-His tag at the C terminal.

The expression was carried out for 8 hours after induction and the expressed product was purified on Ni-NTA column and visualised in SDS-PAGE gel. The expression was clear compared to the non-induced culture.

Larger amount of the protein was prepared as follows.

FNBP production with FNBP(AB)BL21 (PES) Star strain

Culture:

MejliumiFQurJiixeXB .± 2 g/L.gkj£θse_-LiO-0.ug/jαiLAmp, . at 37oC up to OD 1.5, reduce temp to 30 oC, induction with 1 niM IPTG at OD 2-2.5 for 5 hours

Downstream:

Pellet the culture by centrifugation Resuspend in buffer: 10 mM Tris, ρH8, 150 mM NaCl Pellet by centrifugation, resuspend in 40 ml 25 mM Tris pH8 Sonicate 10x3 Os with 1 min paus between. Add PMFS for 5mM final concentration.

Pellet with 20000 g. for 25 min. Supernatant to be saved. Resuspend pellet in 40 ml 25 mM Tris pH8. Sonicate as above. Add PMFS for 5mM final concentration.

Mix supernatant with previous supernatant. Total volume approx. 90 ml.

Apply supernatant on 10 ml Ni-NTA agarose column (with binding capacity 5-10 mg protein/ml gel) equilibrated with sonication buffer (25 mM Tris pH8). Wash with 1 volume sonication buffer.

Wash with 2 volume 25 mM Tris HCl pH: 6.8, 250 ml NaCl Wash with 1 volume 25 mM Tris HCl pH: 8, 8 M Urea Wash with 2 volume sonication buffer. Elute with 200 mM imidazol ρH7. Add TCA to 10% final concentration, put for 1 hour to 4oC. Pellet the protein.

Add 0.2 ml IM Tris HCl pH8 and fill up to 1 ml with TE buffer. Dissolve protein in sonicator with water bath.

(Option: for delivery protein can precipitate with ammonium sulphate.)

A corresponding FnBp A protein was constructed with the same strategy and delivered by Crosslink Ltd. Budapest, Hungary as follows:

- Three tubes containing 2 mg proteins (each). The proteins were purified on Ni- NTA column. The final step was the precipitation with ammonium-sulphate and centrifugation to create the pellet.

What to do: Take up in a weak buffer. Preferably use sonicator with a water bath which promotes dissolving without foam building. Remove the salts on PD-10 column (Amersham).

One tube containing 3.1 mg protein. Same as the other three, but without centrifugation.

What to do: Put the tube at 4oC for one hour. Centrifuge by 15.000 g or more for 20 minutes. Remove the supernatant. Use the same procedure as above.

The protein is a mixture of FnBpA and FnBpB gene with His tag at the end. The Mw is 16IdDa.

FnBpA 1 MEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSF 50

I I 11 I I I I I I i I I I I 11 I I I I I I Il I 11 I I 1 I I I I I I I I I l I I I M I M

FnBpB 1 MEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSF 50 . . . . .

51 EEDTEKDKPKYEHGGNi I DI DFDSVPHIHGFNKHTEI IEEDTNKDKP≤ΎQ ioo

I I I M M I l M I I l I l I l I I l I I I l I l I l I l I I l I l I I l I l I l I l |7| I

51 EEDTEKDKPKYEQGGNI IDIDFDSVPHIHGFNKHTEI IEEDTNKDKPNYQ 100 . . . .

101 FGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPLEHHHHHH* 142

I l I l I l I I l I I l I l I - I I M l I I I l I I I I I I I l I I l I l I I

101 FGGHNSVDFEEDTLPQVSGHNEGQQTIEEDTTPLEHHHHHH* 142

The product was used in the experiments performed in Examples 6 and 7

A corresponding FnBpA protein was produced by Biostapros AB according to the same strategy. Two constructs were made with Cys at the N and C terminal respec- tively. A 50% mixture of each was used in Examples 2-5. FNBP Cys - N

MACEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQN^GNQS FEEDTEKDKPKYEHGGNΠDIDFDSVPHIHGFNKHTEΠEEDTNKDKPSYQFG GHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPG

FNBP Cys -C

MEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFE EDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGH NSVDFEEDTLPKVSGQNEGQQTIEEDTTPCG

Example 2

Introduction

Staphylococcus aureus (SA) is a pathogen for most mammalian species causing acute and chronic infections in wounds including those caused by surgical treat-

ments, in the mammary glands or in man also nosocomial infections. SA is often resistant to antibiotic and the alternative would be a vaccine at least for prophylactic use but, so far, there is no efficient vaccine although many attempts to formulate vaccines have been made including whole cells, toxins, polysaccharides and adhe- sion factors. To succeed with a vaccine formulation against a pathogen with the propensity for causing chronic infections the aid of an adjuvant system is essential when non-replicating antigens are used. Thus, the failure of the various components of SA that are of interest and tested as vaccine antigens including adhesion factors, carbohydrates (polysaccharides) and toxins might be due to the lack of an appropri- ate adjuvant that is acceptable for the species involved. In this example we have chosen an adhesion factor i.e. fibronectin binding protein (FnBp), which is considered to block adhesion but probably more importantly to be target for phgocytosis if right antibodies (opsonizing antibodies) are evoked to FnBp promoting this activity. Furthermore, the FnBp has been complemented with various adjuvant formulations based on the iscom technology including those that are acceptable for sensitive species.

Thus, different animals species require different adjuvant formulations including different saponins. The bovine species e.g. responds very well to a semi-purified saponin of QuilA or Q-WAC types, which might be toxic for other species. Other animal species e.g. cat or mice or man are sensitive to various degree and require a Virtually ^" nδn-toxic formulatiόh ^' as ^' e.gϊ QMDT (QV-MIX ^" Swedish ^" patent applica- ^' tion: 0202110-3 - QV - MIX PCT / SE 03 / 001180) or QWT formulations (QWT Swedish patent application: 0301998-1 PCT / SE 03 / 00586), or a fraction C- ma- trix formulation (ref). The latter is used in veterinary medicine and in clinical trails for man. The goal is a balanced immune response promoting neutralizing as well as antibody class promoting phagocytosis i.e. in the murine species IgG2a, while IgGl is likely to promote anti-adhesion and neutralization effects. In this example these immunological properties are analysed.

It should be noted that SA is an important pathogen for most if not all mammalian species and that the results from a mastitis vaccine has bearing on SA caused diseases in other species including man.

The composition of vaccine antigens might vary in vaccines against SA infections and SA caused disease depending on several factors including local strains, clinical pictures. The adhesion to fibronectin mediates an important factor for the infection process, and this protein is present on virtually all SA isolates. The blocking of adhesion and neutralization is an effect by IgGl by antibodies, while IgG2a is impor- tant for phagocytosis the major immune protective mechanism against SA. The subclasses mentioned reflect the murine immunoglobulin system, while e.g. IgG2a corresponds to IgG3 in the human. The immune response in mice to the strong vaccine candidate FnBp adjuvanted with the QWT- matrixformulation is analysed with regard to the balance between the IgG immunoglobulin classes reflecting T-helper 1 and 2 responses.

Experimental Lay Out

18 g female Balb/c mice were immunised as indicated in Table 1 :1. The antigen dose was determined from earlier experiments to be immunogenic. A low dose was selected, not inducing high responses, in order to see the influence of the added adjuvant. The mice were immunized with either of the FnBp adjuvans formulations 4 weeks apart subcutaneously (s.c.) at the root of the tail. Blood samples for serum testing were taken at weeks 3 and 6. AU animals received lμg of FnBp and the ad- juvant component was in; gr.l non-adjuvanted, gr.2 A1(OH)3; gr.3 Matrix-Q 6μg; gr. 4 Matrix-Q 2 μg. The antigen specific antibody responses in IgGl and IgG2 subclasses at week 6 are shown in figures 1 :1. The antibody levels were measured in ELISA and expressed as the dilution (lOlog) at the OD 450 being the steep part of the dilution curve reading on the Y-axis (Fig 1:1).

Results

After the first immunization the non-adjuvanted and the aluminiumhydroxide- adjuvanted FnBp did hardly induce detectable serum antibody response. The various formulations of FnBp adjuvanted with any of the iscom adjuvanted formulations induced detectable responses to FnBp.

After the second immunization ( ^" Fig. 1:1) the IgGl response was high (>101og 4) in mice groups of mice immunized with FnBp adjuvanted with iscom matrix. In groups of mice given Matrix-Q high or matrixMIX and there was little spread of ti- tres among individual mice while high in the group where the mice were given FnBp in the matrixC formulation. The mice immunized with aluminium hydroxide adjuvanted FnBp responded with significantly lower IgGl serum antibody levels than other adjuvanted FnBp formulations and with large spread of ELISA titres among the individual animals. Mice immunized with the non-adjuvanted formulation responded with a low IgGl response.

After the second immunization (Fig. 1 : 1) the IgG2a response was high (>101og 4) in mice groups of mice immunized with FnBp adjuvanted with iscom matrix. In groups of mice given Matrix-Q high or matrixMIX and there was little spread of titres among individual mice while the spread was high in the group where the mice ^" were giverrFnBp in the matfixC ^" fbrmulatlόli7 ^' The mice immumze ^' d with nόh- adjuvanted FnBp or with aluminium hydroxide adjuvanted FnBp responded with hardly detectable IgG2a serum antibody levels.

Discussion

It is clearly shown that the iscom formulations have capacity to modulate a balanced immune response including both THl and TH2 types of immune responses, which is

essential to induce protection against persistent and chronic infections. So far the adjuvant formulations used in SA vaccines have lacked this capacity

Conclusion

Two interesting formulations for SA and FnBp are the matrixQ suitable for the large animals like the bovines and the Low-Tox matrixC & matrixA in different particles virtually free of side effects and e.g. suitable for cats and human beings. Both these formulations induced high and balanced IgGl and IgG2 responses.

Example 3

Introduction

A vaccine against SA may include or even require various vaccines components in- eluding whole cells, toxins, polysaccharides and adhesion factors, each one contributing to immune protection. In the present example tetanus toxiod (TT) is tested with the different adjuvant formulations as in example 2 to explore their immune enhancing effect on a toxin.

Experimental Lay Out

18 g female Balb/c mice were immunised as indicated in Table 2:1. The antigen dose was determined from earlier experiments to be immunogenic but not inducing very high responses in order to see the influence of the added adjuvant. The mice were immunized with either of the TT adjuvans formulations 4 weeks apart subcu- taneously (s.c.) at the root of the tail. Blood samples for serum testing were taken at weeks 3 and 6. All animals received 0.5 Lf/dose and the adjuvant component was in; gr.l non-adjuvanted, gr.2 A1(OH)3; gr.3 Matrix-Q 6μg; gr. 4 Matrix-Q 2 μg. The antigen specific antibody responses in IgGl and IgG2 subclasses at week 6 are

shown in figures 2:1. The antibody levels were measured in ELISA and expressed as the dilution (lOlog) at the OD 450 being the steep part of the dilution curve reading on the Y-axis (Fig 2:1).

Results

After the first immunization the non-adjuvanted and the aluminiumhydroxide- adjuvanted TT did induce detectable serum antibody response like the various formulations of TT adjuvanted with any of the iscom formulations.

After the second immunization (Fig. 2:1) the IgGl response was high (>101og 4) in all mice groups including non-adjuvanted TT. Slightly higher antibody levels against TT were observed in groups of mice given TT adjuvanted with the iscom matrix formulations and there was little spread of titres among individual mice.

After the second immunization (Fig. 2:1) the IgG2a response was high (>101og 5) in mice groups of mice immunized with TT adjuvanted with iscom matrix. Mice in the groups given TT adjuvanted with matrix -Q or with matrix MIX responded with highest IgG2a levels and there were little spread of titres among the individual mice. The mice immunized with non-adjuvanted TT or with aluminium hydroxide adjuvanted TT responded with no or hardly detectable IgG2a serum antibody levels.

Discussion

TT has a strong antigenic capacity towards a Th2 type of response. Aluminium hy- droxide is also modulating towards the Th2 type of immune response. This example shows that the iscom formulations overcome the strong immune modulatory effect of TT and can induce a balanced ThI -Th2 response to this toxin. It is important for a vaccine against a pathogen inducing chronic or persistent infection to override the intrinsic capacity of this pathogen to modulate the immune response in the host, which the weak adjuvant not can do.

Conclusion

This example shows that the iscom formulations have capacity to modulate immune response of "strong toxin antigens" to a balanced immune response, which can be required to achieve immune protection. Toxins are important pathogenicity factors for SA as well as for other pathogens and might therefore be required to obtain a potent vaccine.

Example 4

SA is producing a number of toxins some of which are strongly immunogenic other are comparatively weakly immunogenic. Any of those may be required vaccine antigens to obtain a vaccine that has very broad protective capacity. Diptheria Toxoid (DT) is a relatively weak antigen in contrast to TT. In this example a weak antigen is tested with the different adjuvant formulations as in example 2 and 3 to explore their immune enhancing effect on a DT type of toxin.

Experimental Lay Out

18 g female Balb/c mice were immunised as indicated in Table 3:1. The antigen dose was determined from earlier experiments to be immunogenic but not inducing very high responses in order to see the influence of the added adjuvant. The mice were immunized with either of the DT adjuvans formulations 4 weeks apart subcu- taneously (s.c.) at the root of the tail. Blood samples for serum testing were taken at weeks 3 and 6. All animals received lμg of DT and the adjuvant component was in; gr.l non-adjuvanted, gr.2 A1(OH)3; gr.3 Matrix-Q 6μg; gr. 4 Matrix-Q 2 μg. The antigen specific antibody responses in IgGl and IgG2 subclasses at week 6 are

shown in figures 3: 1. The antibody levels were measured in ELISA and expressed as the dilution (lOlog) at the OD 450 being the steep part of the dilution curve reading on the Y-axis (Fig 3:1).

Results

After the first immunization only mice immunized with the DT adjuvanted with the Matrix-MIX formulation responded with detectable serum antibody response.

After the second immunization (Fig. 3:1) the IgGl response was highest (> lOlog

3.5) in mice immunized with aluminiumhydoxide- and matrix mixed adjuvanted DT.

After the second immunization (Fig. 3:1) the IgG2a response was high (about lOlog 4) in mice in the group immunized with DT adjuvanted with adjuvanted with matrix MIX responded with the highest antibody levels i.e. almost 50 fold higher titres or more than mice in other groups and the spread of titres was lower. The mice immunized with non-adjuvanted DT or with aluminium hydroxide adjuvanted DT responded with no or hardly detectable IgG2a serum antibody levels.

Discussion

DT is a weaker antigen than TT (example 3) and no-adjuvantes DT did not even after two immunizations induce detectable IgGl or IgG2, i.e. neither a Th2 type of response nor a ThI type. Aluminium hydroxide modulating towards the Th2 type of immune response enhanced the response of DT to readily detectable levels of IgGl . This example shows that the iscom formulations enhance and also modulate the immune response to DT to a balanced response between ThI and 2. Particularly the "low-tox" matrix-MIX was efficient for stimulation of both IgGl and IgG2 i.e. a

potent and balanced immune response. Thus, iscom formulations can efficiently modulate immune responses for both strong and weak antigens.

Conclusion

The examples 2 and 3 show that the iscom formulations efficiently enhance and modulate the immune responses of toxins regardless those are weak or strong antigens and drive e.g. a Th2 biased toxin antigen towards a balanced ThI - Th2 response. Such capacity is important in a complex system of antigens that a SA anti- gen formulation might constitute.

Example 5

Small Scale Field Trial Immunisation Experiment with Staphylococcus Aureus Ad- hesion Factor FnBp Adjuvanted with Iscomatrix

The vaccination experiment at Kungsangen (KA) ₅ a farm belonging to the Agriculture University Uppsala, was carried out to test a potential vaccine against Staphylococcus aureus (SA) in a natural host i.e. the bovine species. Mastitis is a serious problem in diary cattle and also in the related species sheep and goats when those are used for milk production. The mastitis problem is well recognised by anyone experienced in diary science and production including the producer at the diary farm, the veterinarian responsible for the animal health and the factory producing different milk products requiring high quality milk.

To control the situation of mastitis a farm-improved husbandry is the first alternative supplemented with treatment mainly by use of antibiotics. SA is the most important pathogen for the udder causing suffering and economical problems. Particularly problematic is that SA often is antibiotic resistant, and affectd animals have to be culled because there is no treatment. The alternative for treatment of mastitis

with antibiotics is immune prophylactics i.e. the use of vaccine. In spite of many attempts no efficient mastitis vaccine is on the market. The attempts to develop a mastitis SA vaccine have been in vain and that has also been the case with attempts to develop SA vaccines for other specis in other situations e.g. against furunculosis in dogs or against nosocomial infections in human beings. Thus, an efficient vaccine against SA mastitis would rescue affected animals from suffering and have economically extremely good effects for the diary farms, for the diary industry and for their customers.

An adhesion factor, fibronectin binding protein (FnBp), was chosen because it is present in close to 100% of SA isolates. In early experiments (Nelson et al. 1991) the FnBp was incorporated into iscoms. Promising results were obtained with this SA mastitis experimental vaccine. Conversely, continued experiments did not give the desired outcome and the project was discontinued. Reasons for the failure were a short-lived immunity and probably also insufficient immune responses in the mammary gland.

The aim of this vaccination experiment was to explore the possibilities to further develop the FnBp vaccine adjuvanted with a formulation based on the iscom tech- nology. A focus was on an optimal mode of administration to evoke immune responses both systemically, measured in blood serum and locally in the mammary gland. Thus, to obtain a better forecast for the potential of the Vaccine ^~ caTidrdaTe ^" immunological analyses were also carried out on the local secretion (milk whey) from the udder. The evaluation of the immune responses included the antibody re- sponses in serum and milk whey including IgGl, IgG2 and IgA and also looks into the enhancement by antibodies of the phagocytosis of SA cells. IgG2 is promoting phagocytosis, which is the most important defence mechanism against SA. The locally produced IgA antibodies are also important defence factor against invaders in mucosal surfaces. It should be noted that the previous experiments have not ana- lysed the immune responses in the mammary gland most likely because previous

vaccine did not induce clearly detectable immune responses in the milk whey that convincingly would support the trial. It has been conceived that the dilution of high volumes of milk would hide the immune response evoked in the mammary gland.

The lav out of the vaccine trial and Materials and Methods

The fibronectin binding protein (FnBp) of 16 kD fragment of larger a protein to include the DD region responsible for adhesion to fibronectin. The polypetide was expressed in E. coli and obtained from Biostapro AB. It differed from the previous product (Nelson et al. 1991) that was based on shorter 69 to 80 kD protein. Furthermore, in the previous product the FnBp was incorporated into the iscom matrix to form an iscom. In the present experimental vaccine the preparation of the vaccine did not encompass a step to include the FnBp in a matrix to form an iscom. Avoiding the incorporation gives the advantage of a simpler and more economical produc- tion system. Thus, the present formulation differs from two aspects from the previous tested FnBp iscom vaccine candidate.

The iscom matrix was supplied by Isconova AB .

The bacteriological examinations of milk samples were done at the mastitis labora- .tary-atthe ^National ¥eteήnaκy. Institute (SKA),

Experimental performance

The antigen FnBp a rDNA product produced in E. coli was selected because of previous positive experience (Nelson et al-), but the construct was shorter. The immunological evaluation is optimally facilitated with a defined antigen.

Ten heifers going into their first lactation were used as experimental animals to avoid as far as possible previous experience with SA infections, more likely in older

cows, which would cause difficulties for the immunological evaluation of the vaccination.

Three different modes of administration were tested. Since the udder is an organ with a large mucosal area it was considered that mucosal immunity in the mammary gland is important. In contrast to, e.g. man and swine, there is no gut mammary link in the bovines (personal communication Holmberg), i.e. oral administration of vaccine antigens (i.e. in the digestive tract) will evoke immune response in the mammary gland in swine but not in cows. In this experiment two mucosal modes of ad- ministrations were employed, i.e. the primary dose was given by the intranasal (Ln.) or by the intra vaginal (i.vag.) routes. The effect of these modes of priming was compared with the priming by a s.c. immunisation in the supra mammary region. The second dose was administered to all animals by the s.c. route three to four weeks later. With this immunisation regimen it was intended to explore if a link be- tween the upper respiratory region or the genital tract and the mammary gland exist. Important for immune protection is the mucosal (local) immune response and that right type of antibody class is evoked in the mammary gland

Immunisation protocol included a primary immunisation two to four weeks before calving. Three animals were primed by i.n. administration, three animals by i.vag. mode of administration and four animals were immunised by the s.c. route in the supra mania region. The by the s.c. mode in the supra mammal region where lymph nodes draining the mammary gland are located.

Sampling. Blood for serum and immunological analyses were collected at the time of first immunisation and then regularly starting as indicated in the figures (4:1 to 4:2). Collection of milk for bacteriological and immunological analyses of the whey started after partus and then as described for blood.

Bacteriological analyses were carried out at SVA and only at one occasion SA was isolated from a milk sample from one animal primed by the i.n. route. It was just before the second immunisation.

Immunological analyses included measuring of the antibody responses in ELISA (see above). The results are shown in details in Figures 4:1 to 4:4.

Phagocytosis

Isolation and purification of leucocytes from bovine blood was carried out by the

Ficoll centrifugation method as described by Guidry et al 1993 J Dairy Sci 76:1285- 1289 and Lee et al 2005 Can J Vet Res 69:11-18. The cells were resuspended in Hanks basic solution (HBSS), checked for viability and the concentration was adjusted to 10 x 10 ⁶ cells/ml. The selected strain of S. aureus was first cultured on agar plates and subsequently in grown trypticase-soy-broth (TSB) (min 100 ml) in37C forl8 hrs. The bacteria were killed by incubation for 30 min. at 60 C in water bath. After 3 washes saline the bacterial suspension was adjusted in 1:10 dilution at an optical density of 2.0 at 540 nm.

FITC labelling of bacteria was carried out as described by Lee et al 2005 Can J Vet Res 69:11-18. The FITC-labelled bacteria were washed 3 times in Veronal-buffered Saline with ^' O.15 mM ^' calciύm, lmM ^" mag ^" ήe ^" sϊuήf and (F.l% ^" gelafine (GVBSJ me ^~ cόri- ^~ centration was determined at a dilution 1 : 10 to be OD 1.350 at 540 nm and finally kept in GVBS in aliquots of 1 ml at -80C until use.

Phagocytosis; FITC labelled bacteria were thawed and sonicated for 30 sec. At 2.4 A and the concentration was adjusted to 1 x 10 ⁹ /ml. 50 μl of the test sera collected before and after two immunizations undiluted or diluted ¹ A in HBSS were incubated the bacterial suspension and the cells as described by Lee et al 2005 Can J Vet Res 69:11-18. Controls included bacterial suspension incubated in HBSS, and bacterial

suspension incubated with the cell suspension without serum. The incubation was done for 30 min at 37C under careful shaking. Phagocytosis was stopped by adding ice-cold saline with 0.02% EDTA. Before reading in microscope the cells were treated 1% methylene blue to quenching of extra cellular fluorescence. The reading in fluorescence microscope was carried out on a minimum of 200 cells and the proportion of cells with bacteria was determined.

Results

Animals primed by the i.vag. route (Fig 4:l)showed a low or no serum antibody response. In milk whey antibodies to FnBp were recorded after the first immunisation dominated by the IgG2 and IgA subclasses. No milk samples were collected before boost, since there is no milk production before calving. After the s.c. boost the antibody responses to FnBp in serum and milk were of short duration and dominated by IgG2 and IgA.

No or virtually no responses were detected in blood serum from animals primed by the in. route (Fig. 4:2). Antibodies against FnBp in milk whey were mainly recorded in the IgG2 and IgA subclasses. All animals given the primary immunisation by the i.n mode responded. After the s.c. boost the animals responded with clear-cut antibody rises in serum and in whey. The highest responses were in the IgG2 and IgA classes. The antibody levels declined towards the end of the experimental period.

The animals primed and boosted by the s.c. mode of administration obtained IgGl and even higher levels of IgG2 and also high IgA in serum and milk whey (Fig. 4:3). After boost high levels of IgGl and even higher levels of IgG2 and also high IgA in serum and milk whey indicating quality for protective immunity locally in the udder and circulating in the blood. Importantly, the serum antibodies lasted during t he whole experimental period i.e. 7 to 10 months. Also the levels of milk whey anti-

bodies lasted the experimental period i.e. up 8 months indicating that the experimental vaccination will cover the whole lactation period important for a prospective mastitis vaccine.

Phagocytosis was measured on serum from a cow in the group immunized twice by the s.c. route. 30% of PMN cells showed phagocytosis after incubation for 30 min. with bacteria and serum collected before the first immunization. An increase to 51% of PMN cells incubated for 30 min. with bacteria and serum collected after the second immunization showed phagocytosis. 4% PMN cells incubated with bacteria and no serum showed phagocytosis. 2% of PMN cells with no incubation time with bacteria and serum collected after the second immunization showed phagocytosis.

Discussion

The distribution of antibody responses in class and subclasses are of great importance to obtain optimal immune protective effect. Generally the IgG2 antibodies are considered of greatest importance to combat SA infection since this subclass promotes phagocytosis of the bacteria. All modes of immunisation promoted IgG2 and IgA FnBp specific antibodies both in milk whey and in blood serum. The antibody responses were considerably lower in serum and milk whey from animals that were primed i. vag and i.n. Unexpectedly high antibody levels were induced by two s.c. immunizations both in serum and mammary gland secretion with FhBp ^' adjύvaήted ^" with iscom matrix including IgGl ₅ IgG2 and the IgA subclasses. Immune responses of these magnitudes and quality have not been shown before in the bovine mammary gland with SA vaccines or any other vaccine. Also in blood serum these Ig subclasses were recorded. The s.c. mode of administration induced a long-lasting and superior immune response than the previously tested experimental vaccine (Fig 4D), which was of short duration (Nelson et al. 1991). Of particular interest and value is the potent immune response in the mammary gland, which is not described with previous tested vaccine candidates against SA. The result from the functional

phagocytosis test underlines that the important immune protective properties are evoked. The potent immuno enhancing effect of iscom matrix was not expected and facilitates an efficient vaccine production by avoiding the step of conjugation the FnBp polypeptides to iscom matrix.

There are two fibronectin binding protein FnBpA and FnBpB that are similarly organized. The construct used by Nelson et al consisted of a 60-65kD protein conjugated with two IgG binding domains from the Staphylpcoccal protein A to form the fusion protein zzFnBpA to another part containing the presumptive T-cell epitopes to form zz-FnBpA-T and a zz-FnBpB, which contains a presumptive T-cell epitope. These polypeptides were conjugated to iscoms (Lδvgren, K., Lindmark, J., Pipkorn, R. and Morein, B (1987) J. Immunol. Methods 98. We have used these polypetides and found that they fragmented. One reason for the limitation of the Nelson construct we considered to be caused by the tendency to this fragmentation. The present fragment of FnBp of 16kD is more stable and contains the repeats of the important DD region that facilitates the binding to fibronectin.

Conclusion

A truncated FnBp construct supplemented with an iscom matrix formulation induces after two s.crϊmmύmzations high levels of high quality immune responses in the mammary gland and serum. There are strong immunological criteria that the prospective vaccine shall induce immune protection against SA infection in the bovine mammary gland. The technology enables an efficient production system that is of importance for a prospective vaccine.

Example 6

The effect on the whole SA-cell and the α and β haemolysins on the immune responses to FnBp and the toxins

SA causes chronic and persistent infections, which is facilitated by intrinsic properties of the pathogen based on ability to "hide" essential components for infection and survival for the immune system of the host. Moreover, pathogens causing persistent and chronic infections have capacity to guide the immune reactions of the host to allow its persistence. SA harbour a number of different components of immune regulatory properties and a wrong composition may cause low immune responses to one or all antigens included in the vaccine composition. As described above there are many components that are candidates for a SA vaccine. The first strategy is to use components that are isolated form the pathogen or produced as iso- lates vaccine antigens expressed by a cell in this case E. coli. The second approach is to use a powerful adjuvant in this case the iscom matrix. One prospective class of vaccine components are toxins. The fact that both α and β haemolysins (for the β haemolysin in 100% of the cases) of SA very frequently are identified in SA isolates from mastitis cases makes them strong vaccine candidates. Both toxins are excreted products and can be collected from the culture fluid where SA is growing. In this experiment the SA cells and culture fluid containing the α and β haemolysins were mixed with FnBp in a vaccine composition as laid out in Materials and Methods to explore the immunogenicity of FnBp and the toxins in this composition.

Materials and Methods

Experimental lay out. Twenty-five calves stationed at Oveds Kloster in the south of Sweden in the age of 4 to 7 months were immunized three times with 4 weeks intervals according to the immunization schedule below.

The SA bacteria and the culture fluid containing the α and β haemolysins were produced and kindly supplied by Karolinska Institutet MTC section of bacteriology headed by Professor Roland Mδllby.

The immunizations were carried out s.c. prescapular at the side of the neck with 200μg of FnBp or the mixture of FnBp and 10 exp 8/ dose of Staphylococcus aureus cells and 50μg each of the α and β haemolysins as described in the text table below. All vaccine formulations were adjuvanted with 1 mg iscom matrix per dose. The dose volume was 2 ml.

IMMUNIZATION SCHEME Test against animal groups

A/ FnBp FnBp FnBp 5 FnBp & toxins

B/ SA FnBp FnBp 5 FnBp & toxins

C/ SA SA SA 5 FnBp & toxins

D/ SA+Fn SA+Fn SA+Fn 5 FnBp & toxins

E/ PBS PBS PBS 5 FnBp & toxins

Fn: FnBp, S: Staphylococcus aureus cells and the α and β haemolysins, PBS: Phosphate buffered solution

The third immunizations are done for additional information about reaching the ceiling of the immune response, and information about possible suppres- sion after repeated immunizations.

The evaluation of antibody responses to FnBp was carried out on serum by ELISA as described above. We need to know if SA as whole cell + toxins have a dominating effect on FnBp i.e. inhibit the immune response against FnBp.

Neutralization tests against the α and β haemolysins were carried out at the Karolinska Institutet MTC section of bacteriology headed by Professor Roland Mollby.

Results

The calves in this example are under one year of age and they did not respond or responded with very low antibody titres to FnBp supplemented with SA cells and the α and β haemolysins.

The immune response to FnBp measured by ELISA of group 1 is presented in figure 5:1. After the first immunization only animals in group 1 immunized with FnBp only and adjuvanted with iscom matrixresponded both with IgM and IgG antibodies.

After the first boost clear-cut increases of the IgM, IgGl and IgG2 responses were recorded in the animals vaccinated with FnBp alone. No animals primed with a composition containing FnBp and the Staphylococcus aureus cells and the α and β haemolysins showed immune response to FnBp (not shown).

After the third immunization tests to be done.

The immune response to α and β haemolysins were measured in a functional i.e. toxin neutralisation tests. The results are shown in figure 5:2. After the first immunization a low IgG response was recorded in animals in groups vaccines containing SA cells and toxins i.e. B ₅ C and D. The antibody levers declined at the time for the second immunization. After the second immuni-

zation animals that have received two doses of toxins responded with high neutralization titres i.e. animals in groups C and D.

Discussion

This experiment shows that a mixture of Staphylococcus aureus cells and the α and β haemolysins suppresses the immune response to FnBp. In contrast all animals responded with high neutralizing antibodies against the α and β haemolysins. It is not clear, whether the toxins or other products in culture fluid or the whole cells or the combination of both cause the down regulation. The animals in this experiment were under one year of age. In a later experiment the animals were over one year up to two years old and these animals, which received FnBp supplemented either with SA cells or the α and β haemolysins, responded with high antibodies to FnBp (see example 7).

The suppression noticed in this experiment seems to be based on an active immune response i.e. with memory, since the animals in group B did not respond to the second dose.

In contrast the α and β haemolysins induced strong immune responses in all vaccine antigen combinations tested, which suggests that the combination of toxins and FnBp is a compatible and feasible vaccine composition. The possible use of whole SA cells in a vaccine composition needs further investigation. The results from example 7 indicates that older calves or cows might not react with a suppression to a vaccine antigen when SA cells or the α and β haemolysins are included in the vaccine formulation.

Conclusion

Previous experiments have shown that FnBp adjuvanted with iscom matrix is highly immunogenic in the bovine species, which is confirmed in this exam- pie. In the present example the immune response to FnBp is down regulated when FnBp is supplemented both with SA cells and the α and β haemolysins. It can be concluded from example 7 that a vaccine composition combining FnBp and the α and β haemolysins is highly immunogenic and so is a vaccine combination FnBp SA cells, when the antibody response is measured against FnBp. It is conceivable that, cattle over the age of one year, do not react with the suppression noted in younger animals immunized with FnBp supplemented SA cells in combination with the α and β haemolysins.

Example 7

In example 6 we have seen that a mixture of α and β haemolysins and whole SA cells in an experimental vaccine down-regulated the immune response to the third component i.e. FnBp. Since both α and β haemolysins and whole SA cells were included it was not clear whether one or the other or the com- bination of both toxins and cells were responsible for the suppressive effect. In this example the suppressive respectively the compatible or enhancing effects are further investigated by combining FnBp with either the toxins or the whole SA cells. For that purpose an experiment was set up as described in materials and methods. All experimental vaccines were adjuvanted with is- com matrix .

Materials and Methods

Experimental lay out. 15 calves, stationed at the Mosta Farm east of Uppsala in Sweden, in the age of 1 to 2 years were immunized two times with 6 weeks intervals according to the immunization schedule below.

The SA bacteria and the culture fluid containing the α and β haemolysins were produced and kindly supplied by Karolinska Institutet MTC section of bacteriology headed by Professor Roland Mδllby.

The immunizations were carried out s.c. prescapular at the side of the neck with 200 μg of FnBp or the mixture of FnBp and 10 exp 8/ dose of Staphylococcus aureus cells or the mixture of FnBp with 50μg each of the α and β haemolysins as described in the text table below. All vaccine formulations were adjuvanted with 1 mg iscom matrix per dose. The dose volume was 2 ml.

IMMUNIZATION SCHEME Test against animal groups

A/ FnBp FnBp 5 FnBp & toxins

B/ ^" SA+Fn SA+Fn ^" 5 FnBp & toxins

C/ Tox+Fn Tox+Fn 5 FnBp & toxins

D/ PBS PBS 5 FnBp & toxins

Fn: FnBp, SA: Staphylococcus aureus, Tox: the α and β haemolysins PBS: Phosphate buffered solution

Results

The immune response to FnBp measured by ELISA is presented in figure 6:1. Three weeks after the first immunization all animals responded with comparatively high antibody titres against FnBp including all immunoglobulin classes tested i.e. IgM, IgGl and IgG2.

With such a primary immune response all accumulated experience tell us that after the second immunisation a substantial increase of the IgG subclass re- sponse will occur. The data from the second immunization are pending.

Discussion

This experiment shows that a mixture of Staphylococcus aureus cells and the α and β haemolysins separately do not suppress the immune response to

FnBp. In example 6 it was clearly shown that the toxin component or other products in culture fluid and the whole cells in combination down regulated the immune response in all immnunoglobulin classes tested. The suppression demonstrated in example 6 seems to be based on an active immune response including immune memory cells.

Tn example 6 ^" ft was also shown that the α and β haemolysins induced strong immune responses in all vaccine antigen combinations, which suggests that the combination of toxins and FnBp is a compatible and feasible vaccine composition. The results from this example shows that the use of whole SA cells in a vaccine composition is possible provided the factor(s) causing suppression in the vaccine antigen composition tested in some groups in example 6 are identified and excluded from the prospective vaccine containing whole cells. It should be noted that the antibody response to FnBp supplemented with both SA cells and the α and β haemolysins is low indicating a suppres-

sion of immune response to FnBp. It should be noted that the calves in example 6 were under one year of age, while the animals in this example 7 were older i.e. 1 to 2 years. The younger animals might still have a not fully developed immune system or maternal antibodies not reflected by FnBp antibod- ies.

In a number of formulations the iscom matrix adjuvanted experimental vaccines have included FnBp and toxins (see examples 3 and 4). In mouse models very potent immune responses were evoked both to FnBp and the in- eluded toxins. Thus, the accumulated results strongly show that strong immune responses are induced by prospective vaccine formulations including an adhesion factor and other components adjuvanted with iscom formulations.

Conclusion

High antibody levels were induced in animals in the age to go into lactation against FnBp when mixed either with toxins or with whole SA bacterial cells. Thus, a SA vaccine based on several antigen components is feasible.

Table 1:1

Lay out of the immunization of Balb/C mice with fibronectin binding protein (FnBp). The mice were immunized twice s.c. four weeks apart with the respective vaccine candidates

LowTox formulation consists of a mixture of 0,6 ug Matrix-C + 3,4 ug Matrix-A in separate particles.

Table 2:1

Lay out of the immunization of Balb/C mice tetanus toxiod (TT). The mice were immunized twice s.c four weeks apart with the respective vaccine candidates

LowTox formulation consists of a mixture of 0,6 ug Matrix-C + 3,4 ug Matrix-A in separate particles.

Table 3:1

Lay out of the immunization of Baib/C mice with difteri tσxiod (DT). The mice- were immunized twice s.c. four weeks apart with the respective vaccine candidates

LowTox formulation consists of a mixture of 0,6 ug Matrix-C + 3,4 ug Matrix-A in separate particles.