Title:
VARIANT ACYL-COA HYDROLASE
Document Type and Number:
WIPO Patent Application WO/2022/181654
Kind Code:
A1
Abstract:
The present invention addresses the problem of improving the capacity for producing 3-hydroxyadipic acid and/or α-hydromconic acid by using variant acyl-CoA hydrolase or bacteria with a nucleic acid that codes for the variant acyl-CoA hydrolase introduced thereto. Provided is a variant acyl-CoA hydrolase having acyl-CoA-hydrolase activity, comprising a polypeptide having the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide having an amino acid sequence having at least 60% sequence identity with the amino acid sequence represented by SEQ ID NO: 1 and having acyl-CoA hydrolase activity, wherein a portion of the amino acids has been substituted. Or, provided is a microorganism with a nucleic acid that codes for said variant acyl-CoA hydrolase introduced thereto.
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Inventors:
NAKAMURA HITOMI (JP)
ISOBE KYOHEI (JP)
KAWAMURA KENJI (JP)
YAMADA KATSUSHIGE (JP)
SABURI HAJIME (JP)
TERAOKA YOSHIAKI (JP)
KAMEDA TOMOSHI (JP)
IKEBE KIMIYOSHI (JP)
ISOBE KYOHEI (JP)
KAWAMURA KENJI (JP)
YAMADA KATSUSHIGE (JP)
SABURI HAJIME (JP)
TERAOKA YOSHIAKI (JP)
KAMEDA TOMOSHI (JP)
IKEBE KIMIYOSHI (JP)
Application Number:
PCT/JP2022/007457
Publication Date:
September 01, 2022
Filing Date:
February 24, 2022
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
AIST (JP)
AIST (JP)
International Classes:
C12N15/55; C12N1/15; C12N1/19; C12N1/21; C12N9/16; C12N15/31; C12P7/44
Domestic Patent References:
| WO2019107516A1 | 2019-06-06 |
Foreign References:
| JP2011515111A | 2011-05-19 | |||
| JP2011512868A | 2011-04-28 |
Other References:
DATABASE PROTEIN 30 July 2018 (2018-07-30), ANONYMOUS : "acyl-CoA thioesterase [Escherichia coli]", XP055961708, retrieved from NCBI Database accession no. STM59535
DATABASE PROTEIN 19 May 2022 (2022-05-19), ANONYMOUS : "acyl-CoA thioesterase II [Serratia grimesii]", XP055961704, retrieved from NCBI Database accession no. WP_037425284
JURGEN NAGGERT, MEENA L NARASIMHANG, LINDA DEVEAUXG, HYESEON CHOG, ZAFAR I RANDHAWAB, JOHN E CRONAN, BRIAN N GREENLL, STUART SMITH: "Cloning, Sequencing, and Characterization of Escherichia coli Thioesterase 11", S. A, 15 June 1991 (1991-06-15), pages 11044 - 11050, XP055271646, Retrieved from the Internet
KALLSCHEUER NICOLAI; GäTGENS JOCHEM; LüBCKE MARVIN; PIETRUSZKA JöRG; BOTT MICHAEL; POLEN TINO: "Improved production of adipate withEscherichia coliby reversal of β-oxidation", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 101, no. 6, 8 December 2016 (2016-12-08), Berlin/Heidelberg, pages 2371 - 2382, XP036164229, ISSN: 0175-7598, DOI: 10.1007/s00253-016-8033-3
DATABASE PROTEIN 19 May 2022 (2022-05-19), ANONYMOUS : "acyl-CoA thioesterase II [Serratia grimesii]", XP055961704, retrieved from NCBI Database accession no. WP_037425284
JURGEN NAGGERT, MEENA L NARASIMHANG, LINDA DEVEAUXG, HYESEON CHOG, ZAFAR I RANDHAWAB, JOHN E CRONAN, BRIAN N GREENLL, STUART SMITH: "Cloning, Sequencing, and Characterization of Escherichia coli Thioesterase 11", S. A, 15 June 1991 (1991-06-15), pages 11044 - 11050, XP055271646, Retrieved from the Internet
KALLSCHEUER NICOLAI; GäTGENS JOCHEM; LüBCKE MARVIN; PIETRUSZKA JöRG; BOTT MICHAEL; POLEN TINO: "Improved production of adipate withEscherichia coliby reversal of β-oxidation", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 101, no. 6, 8 December 2016 (2016-12-08), Berlin/Heidelberg, pages 2371 - 2382, XP036164229, ISSN: 0175-7598, DOI: 10.1007/s00253-016-8033-3
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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