Title:
VARIANT OF FORMATE DEHYDROGENASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/169341
Kind Code:
A1
Abstract:
A variant protein of a formate dehydrogenase, which has a β-nicotinamide-adenine-dinucleotide-phosphate-dependent formate dehydrogenase activity, can be constructed by introducing a site-specific mutation into an aspartic acid residue located at position-222 in the amino acid sequence for a formate dehydrogenase variant originated from Mycobacterium vaccae. The protein exhibits a high formate dehydrogenase activity in the presence of an organic solvent. The variant is useful for the production of an alcohol using a ketone as a starting material.
Inventors:
UEDA MOMOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
Application Number:
PCT/JP2012/063059
Publication Date:
December 13, 2012
Filing Date:
May 22, 2012
Export Citation:
Assignee:
DAICEL CORP (JP)
UEDA MOMOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
UEDA MOMOKO (JP)
YAMAMOTO HIROAKI (JP)
HAYASHI MOTOKO (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N9/04; C12P7/02; C12P13/00; C12P19/36
Domestic Patent References:
| WO2011147957A1 | 2011-12-01 |
Foreign References:
| JP2002223776A | 2002-08-13 | |||
| JP2004041062A | 2004-02-12 |
Other References:
K. HOLSCH ET AL.: "Enantioselective reduction of prochiral ketones by engineered bifunctional fusion proteins.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol. 56, 2010, pages 131 - 140
DATABASE DDBJ/EMBL/GENBANK 6 October 2001 (2001-10-06), GALKIN, A. ET AL.: "Definition: Mycobacterium vaccae fdh gene for formate dehydrogenase, complete cds.", retrieved from http: //www.ncbi.nlm.nih.gov/nuccore/ab072394 accession no. B072394
GALKIN A. ET AL.: "Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10.", APPL MICROBIOL BIOTECHNOL., vol. 44, no. 3-4, 1995, pages 479 - 483
YAMAMOTO H. ET AL.: "Robust NADH-regenerator: improved alpha-haloketone-resistant formate dehydrogenase.", APPL MICROBIOL BIOTECHNOL., vol. 67, no. 1, 2005, pages 33 - 39
DATABASE DDBJ/EMBL/GENBANK 6 October 2001 (2001-10-06), GALKIN, A. ET AL.: "Definition: Mycobacterium vaccae fdh gene for formate dehydrogenase, complete cds.", retrieved from http: //www.ncbi.nlm.nih.gov/nuccore/ab072394 accession no. B072394
GALKIN A. ET AL.: "Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10.", APPL MICROBIOL BIOTECHNOL., vol. 44, no. 3-4, 1995, pages 479 - 483
YAMAMOTO H. ET AL.: "Robust NADH-regenerator: improved alpha-haloketone-resistant formate dehydrogenase.", APPL MICROBIOL BIOTECHNOL., vol. 67, no. 1, 2005, pages 33 - 39
Attorney, Agent or Firm:
SHIMIZU, Hatsushi et al. (JP)
Spring water Original aim (JP)
Spring water Original aim (JP)
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Claims: