Title:
VARIANT ISPH AND METHOD FOR SYNTHESIZING COMPOUND HAVING, AS STRUCTURAL UNIT, ISOPRENE OBTAINED BY USING SAID VARIANT ENZYME
Document Type and Number:
WIPO Patent Application WO/2022/102763
Kind Code:
A1
Abstract:
As a result of determining an enzyme that is a limiting factor for improving the amount of isoprenoids synthesized in a MEP pathway, the present invention provides: a variant of said enzyme; a method for synthesizing a hydrocarbon having, as a structural unit, an isoprene obtained by using a MEP pathway in which said limiting factor is resolved; and a screening system for an enzyme involved in an isoprenoid pathway using an isoprenoid pathway expression system. According to the present invention, it has been identified that IspH is an enzyme that is a limiting factor for improving the amount of isoprenoids synthesized in a MEP pathway. Furthermore, it has been confirmed that the amount of isoprenoids synthesized in a MEP pathway including a variant of said IspH is high.
Inventors:
UMENO DAISUKE (JP)
Application Number:
PCT/JP2021/041812
Publication Date:
May 19, 2022
Filing Date:
November 12, 2021
Export Citation:
Assignee:
UNIV CHIBA NAT UNIV CORP (JP)
International Classes:
C12N15/53; C12N9/04; C12P5/02; C12Q1/26
Foreign References:
| US20040146966A1 | 2004-07-29 |
Other References:
RAO GUODONG, OLDFIELD ERIC: "Structure and Function of Four Classes of the 4Fe–4S Protein, IspH", BIOCHEMISTRY, vol. 55, no. 29, 26 July 2016 (2016-07-26), pages 4119 - 4129, XP055930567, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.6b00474
KIA-JOO PUAN; HONG WANG; TOHRU DAIRI; TOMOHISA KUZUYAMA; CRAIG T. MORITA: "fldA is an essential gene required in the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway for isoprenoid biosynthesis", FEBS LETTERS, vol. 579, no. 17, 13 June 2005 (2005-06-13), NL , pages 3802 - 3806, XP071246395, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2005.05.047
KIA-JOO PUAN; HONG WANG; TOHRU DAIRI; TOMOHISA KUZUYAMA; CRAIG T. MORITA: "fldA is an essential gene required in the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway for isoprenoid biosynthesis", FEBS LETTERS, vol. 579, no. 17, 13 June 2005 (2005-06-13), NL , pages 3802 - 3806, XP071246395, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2005.05.047
Attorney, Agent or Firm:
SHOJI Takashi et al. (JP)
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