Title:
VARIANTS OF A DNA POLYMERASE OF THE POLX FAMILY
Document Type and Number:
WIPO Patent Application WO/2017/216472
Kind Code:
A3
Abstract:
The invention relates to variants of a DNA polymerase of the polX family capable of synthesising a nucleic acid molecule without a template strand, or of a functional fragment of such a polymerase, comprising at least one mutation of a residue in at least one specific position, and to the uses of said variants, in particular for the synthesis of nucleic acid molecules comprising 3'- OH modified nucleotides.
Inventors:
YBERT THOMAS (FR)
DELARUE MARC (FR)
DELARUE MARC (FR)
Application Number:
PCT/FR2017/051519
Publication Date:
March 15, 2018
Filing Date:
June 13, 2017
Export Citation:
Assignee:
DNA SCRIPT (FR)
PASTEUR INSTITUT (FR)
CENTRE NAT RECH SCIENT (FR)
PASTEUR INSTITUT (FR)
CENTRE NAT RECH SCIENT (FR)
International Classes:
C12N9/12; C12P19/34
Domestic Patent References:
| WO2016064880A1 | 2016-04-28 |
Other References:
BAOLI YANGS ET AL: "Mutational Analysis of Residues in the Nucleotide Binding Domain of Human Terminal Deoxynucleotidyl Transferase*", 22 April 1994 (1994-04-22), pages 11859 - 11868, XP055345779, Retrieved from the Internet [retrieved on 20170214]
M. DELARUE ET AL: "Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase", EMBO JOURNAL, vol. 21, no. 3, 1 February 2002 (2002-02-01), pages 427 - 439, XP002767368, DOI: 10.1093/emboj/21.3.427
DATABASE Geneseq [online] 11 September 1995 (1995-09-11), YANG B ET AL: "T-cell specific avian Td: Characterization of the cDNA and recombinant enzyme", XP002774865, Database accession no. aaa75280
M. DELARUE ET AL: "Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase", EMBO JOURNAL, vol. 21, no. 3, 1 February 2002 (2002-02-01), pages 427 - 439, XP002767368, DOI: 10.1093/emboj/21.3.427
DATABASE Geneseq [online] 11 September 1995 (1995-09-11), YANG B ET AL: "T-cell specific avian Td: Characterization of the cDNA and recombinant enzyme", XP002774865, Database accession no. aaa75280
Attorney, Agent or Firm:
LEBRETTE, Camille et al. (FR)
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