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Title:
VIBRIO UNIVERSAL SUICIDE VECTOR FOR GENE KNOCKOUT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/045281
Kind Code:
A1
Abstract:
Provided are a vibrio universal suicide vector for gene knockout, a construction method thereof and a use thereof in the vibrio gene knockout. The suicide vector pLP12 is a circular vector comprising a P BAD promoter, a repressor protein gene araC, an RP4 transfer initiation site, a chloramphenicol resistance gene, an R6K replication initiation site, a multiple cloning site region, and a lethal gene vmi480, wherein the multiple cloning site region comprises at least two AhdI enzyme cleavage sites. The suicide vector pLP12 is digested with AhdI to form a linearized suicide vector pLP12T.

Inventors:
LUO, Peng (No.164, West Xingang Road Haizhu Distric, Guangzhou City Guangdong 1, 510301, CN)
HE, Xiangyan (No.164, West Xingang Road Haizhu Distric, Guangzhou City Guangdong 1, 510301, CN)
LIU, Qiuting (No.164, West Xingang Road Haizhu Distric, Guangzhou City Guangdong 1, 510301, CN)
HU, Chaoqun (No.164, West Xingang Road Haizhu Distric, Guangzhou City Guangdong 1, 510301, CN)
Application Number:
CN2015/097202
Publication Date:
March 23, 2017
Filing Date:
December 11, 2015
Export Citation:
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Assignee:
SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY, CHINESE ACADEMY OF SCIENCES (No.164, West Xingang Road Haizhu Distric, Guangzhou City Guangdong 1, 510301, CN)
International Classes:
C12N15/63; C12N1/20; C12N15/03; C12R1/63
Foreign References:
CN105063073A2015-11-18
CN104817632A2015-08-05
Other References:
LUO, PENG ET AL.: "Developing Universal Genetic Tools for Rapid and Efficient Deletion Mutation in Vibrio Species Based on Suicide T-Vectors Carrying a Novel Counterselectable Marker, vmi480", PLOS ONE, vol. 10, no. 12, 7 December 2015 (2015-12-07), pages 1 - 17, XP055373177, ISSN: 1932-6203
ROUX, F.L. ET AL.: "Construction of a Vibrio splendidus Mutant Lacking the Metalloprotease Gene vsm by Use of a Novel Counterselectable Suicide Vector", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 3, 28 February 2007 (2007-02-28), pages 777 - 784, XP002674498, ISSN: 0099-2240
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (Room 616, 6/F Building 23-1, No.100, Xianlie Central Road, Yuexiu Distric, Guangzhou City Guangdong 0, 510070, CN)
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