Title:
VIRUS-DERIVED CONSTRUCT PLASMID LIBRARY AND CONSTRUCTION OF SAME
Document Type and Number:
WIPO Patent Application WO/2023/214578
Kind Code:
A1
Abstract:
In one aspect, the present disclosure provides a method for producing various virus-derived construct plasmids from starting plasmids (for example, the virus-derived construct plasmids of the present disclosure). In one embodiment, the method for producing virus-derived construct plasmids according to the present disclosure includes: a step for preparing a set of starting plasmids containing at least a portion of a nucleic acid sequence necessary for forming a virus-derived construct, the set of starting plasmids including a set of corresponding alternative unit nucleic acids, and the set of corresponding alternative unit nucleic acids including at least one set of corresponding alternative unit nucleic acids that contain a different nucleic acid sequence; a step for treating the set of starting plasmids with a restriction enzyme and preparing a mixed solution containing cleaved alternative unit nucleic acid fragments; a step for ligating the cleaved alternative unit nucleic acid fragments to form an integrated nucleic acid; and a step for causing the integrated nucleic acid to contact a transformed organism and thereby forming virus-derived construct plasmids.
Inventors:
SAITO SHUNSUKE (JP)
TSUGE KENJI (JP)
NISHIMURA YUYA (JP)
TSUGE KENJI (JP)
NISHIMURA YUYA (JP)
Application Number:
PCT/JP2023/017130
Publication Date:
November 09, 2023
Filing Date:
May 02, 2023
Export Citation:
Assignee:
SYNPLOGEN CO LTD (JP)
International Classes:
C12N15/86; A61K35/76; A61K48/00; A61P43/00; C12N5/10; C12N7/01; C12N15/12; C12N15/37; C12N15/48; C12N15/52; C12N15/63; C12N15/66; C12N15/861; C12N15/863; C12N15/864; C12N15/867; C12N15/869; C40B40/08
Domestic Patent References:
| WO2020203496A1 | 2020-10-08 | |||
| WO2022097646A1 | 2022-05-12 |
Foreign References:
| JP2021502799A | 2021-02-04 | |||
| JP2020188763A | 2020-11-26 | |||
| JP2002528049A | 2002-09-03 | |||
| US20180155740A1 | 2018-06-07 | |||
| JP2021510496A | 2021-04-30 |
Other References:
TSUGE KENJI, MATSUI K, ITAYA M: "One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid.", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 31, no. 21, 1 November 2003 (2003-11-01), GB , pages E133.1 - E133.8, XP002569369, ISSN: 0305-1048, DOI: 10.1093/NAR/GNG133
CHRISTOPHER VOIGT (ED.): "Computer aided design and DNA assembly: Synthetic biology, Pt. B IN: Methods in Enzymology; ISSN 0076-6879; Vol. 498", vol. 498, 1 January 2011, ELSEVIER, ACAD. PRESS , NL , ISBN: 978-0-12-385120-8, article MITSUHIRO ITAYA, TSUGE KENJI, VOIGT CHRISTOPHER A.: "Construction and Manipulation of Giant DNA by a Genome Vector", pages: 427 - 447, XP055355825, DOI: 10.1016/B978-0-12-385120-8.00019-X
CHRISTOPHER VOIGT (ED.): "Computer aided design and DNA assembly: Synthetic biology, Pt. B IN: Methods in Enzymology; ISSN 0076-6879; Vol. 498", vol. 498, 1 January 2011, ELSEVIER, ACAD. PRESS , NL , ISBN: 978-0-12-385120-8, article MITSUHIRO ITAYA, TSUGE KENJI, VOIGT CHRISTOPHER A.: "Construction and Manipulation of Giant DNA by a Genome Vector", pages: 427 - 447, XP055355825, DOI: 10.1016/B978-0-12-385120-8.00019-X
Attorney, Agent or Firm:
YAMAMOTO, Shusaku et al. (JP)
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