Title:
VIRUS TRANSFECTION SYNERGIST AND VIRUS TRANSFECTION APPLICATIONS BASED ON CLICK CHEMISTRY
Document Type and Number:
WIPO Patent Application WO/2019/014924
Kind Code:
A1
Abstract:
Related are a virus transfection synergist—a DBCO-modified cationic polymer PEI (PEI-DBCO) and applications thereof in virus transfection, specifically, virus transfection applications based on click chemistry, and a preparation method for the virus transfection synergist. Also related are a virus transfection vector system, which is a virus-PEI-DBCO complex formed by PEI-DBCO being adsorbed on the surface of virus particles, is provided on the surface with a DBCO group and a positive charge, and effectively increases virus transfection efficiency, and applications of the virus transfection vector system. Finally, related are a mammalian cell transfection method for implementing high-efficiency loading of a mammalian cell virus and cell transfection via a bioorthogonal reaction, and applications of an azide modification of a cell in mammalian cell transfection.
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Inventors:
CAI LINTAO (CN)
MA YIFAN (CN)
PAN HONG (CN)
JIN YAN (CN)
LI PING (CN)
LI WENJUN (CN)
MA YIFAN (CN)
PAN HONG (CN)
JIN YAN (CN)
LI PING (CN)
LI WENJUN (CN)
Application Number:
PCT/CN2017/093870
Publication Date:
January 24, 2019
Filing Date:
July 21, 2017
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12N15/11; C08J3/24
Domestic Patent References:
| WO2016123365A1 | 2016-08-04 |
Foreign References:
| CN104749369A | 2015-07-01 | |||
| CN101755048A | 2010-06-23 | |||
| CN103588998A | 2014-02-19 | |||
| CN104730253A | 2015-06-24 |
Other References:
BOUSSIF, O. ET AL.: "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine", PROC.NATL.ACAD.SCI., vol. 92, 31 August 1995 (1995-08-31), pages 7297 - 7301, XP002947333
LIM, YONG-BEOM ET AL.: "Cationic Hyperbranched Poly (amino ester): A Novel Class of DNA Condensing Molecule with Cationic Surface, Biodegradable Tme.Di.hreensional Structure, and Tertiary Amine Groups in the Interior", J.AM.CHEM.SOC., vol. 123, 16 February 2001 (2001-02-16), pages 2460 - 2461, XP055568037
LIM, YONG-BEOM ET AL.: "Cationic Hyperbranched Poly (amino ester): A Novel Class of DNA Condensing Molecule with Cationic Surface, Biodegradable Tme.Di.hreensional Structure, and Tertiary Amine Groups in the Interior", J.AM.CHEM.SOC., vol. 123, 16 February 2001 (2001-02-16), pages 2460 - 2461, XP055568037
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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