Title:
VOLUME EXCLUSION AGENT TO ENHANCE FORMATION OF EXTRACELLUAR MATRIX
Document Type and Number:
WIPO Patent Application WO/2010/027789
Kind Code:
A3
Abstract:
A method of enhancing the formation of extracellular matrix in culture. Cells in culture secrete most of the collagen into the media as unprocessed procollagen, i.e., the cells do not convert procollagen to collagen. In contrast, normal extracellular matrix deposition involves procollagen processing to collagen, fibril assembly and deposition into the cell layer to form a collagenous extracellular matrix. The addition of certain growth factors and the addition of a thin layer of a certain volume exclusion agent on top of the cells dramatically enhances the conversion of procollagen to collagen and will increase the amount of collagen and extracellular matrix associated with the cells. This invention advances bioengineering of connective tissues for medical applications that require an extensive and functional extracellular matrix with high tensile strength such as those in the cornea stroma, skin, tendons, ligaments, articular cartilage and the intervertebral disks.
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Inventors:
BIRK DAVID E (US)
HASSELL JOHN (US)
KANE BRADLEY (US)
ETHEREDGE LA TIA (US)
HASSELL JOHN (US)
KANE BRADLEY (US)
ETHEREDGE LA TIA (US)
Application Number:
PCT/US2009/054899
Publication Date:
June 24, 2010
Filing Date:
August 25, 2009
Export Citation:
Assignee:
UNIV SOUTH FLORIDA (US)
UNIV JEFFERSON (US)
BIRK DAVID E (US)
HASSELL JOHN (US)
KANE BRADLEY (US)
ETHEREDGE LA TIA (US)
UNIV JEFFERSON (US)
BIRK DAVID E (US)
HASSELL JOHN (US)
KANE BRADLEY (US)
ETHEREDGE LA TIA (US)
International Classes:
C12N5/02; C12N5/071
Foreign References:
| US5827641A | 1998-10-27 |
Other References:
CHAIPINYO, K. ET AL.: "Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels.", J. ORTHOPAEDIC RES., vol. 20, 2002, pages 1070 - 1078
BRYANT, S. J. ET AL.: "Hydrogel properties influence ECM production by dhcondrocytes photoencapsulated in poly(ethylene glycol)hydrogels.", J. BIOMED. MATER. SCI., vol. 59, 2002, pages 63 - 72
HASSELL, J.R. ET AL.: "Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose.", EXP. EYE RES., vol. 87, 7 October 2008 (2008-10-07), pages 604 - 611
BRYANT, S. J. ET AL.: "Hydrogel properties influence ECM production by dhcondrocytes photoencapsulated in poly(ethylene glycol)hydrogels.", J. BIOMED. MATER. SCI., vol. 59, 2002, pages 63 - 72
HASSELL, J.R. ET AL.: "Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose.", EXP. EYE RES., vol. 87, 7 October 2008 (2008-10-07), pages 604 - 611
Attorney, Agent or Firm:
MCGAW, Michael, M. (Oldsmar, Florida, US)
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