Title:
WEST NILE VIRUS VACCINE
Document Type and Number:
WIPO Patent Application WO/2005/014803
Kind Code:
A1
Abstract:
It is intended to provide process for producing a West Nile Virus (WNV) vaccine and an attenuated WNV vaccine produced by this method. A process which comprises culturing an established cell line (Vero cells) originating in the kidney of a WNV-inoculated vervet monkey at a high density, attenuating the WNV-infected cells or a liquid culture medium thereof thus obtained, and then highly purifying WNV by sucrose density gradient centrifugation; WNV obtained by this process; and an attenuated WNV vaccine comprising the WNV or an antigenic component thereof as the main component and being substantially free from any nucleic acids or proteins originating in a host.
Inventors:
SONODA KENGO (JP)
ISHIKAWA YUJI (JP)
NISHIYAMA KIYOTO (JP)
KUZUHARA SHOJI (JP)
KINO YOICHIRO (JP)
ISHIKAWA YUJI (JP)
NISHIYAMA KIYOTO (JP)
KUZUHARA SHOJI (JP)
KINO YOICHIRO (JP)
Application Number:
PCT/JP2004/011041
Publication Date:
February 17, 2005
Filing Date:
August 02, 2004
Export Citation:
Assignee:
CHEMO SERO THERAPEUT RES INST (JP)
SONODA KENGO (JP)
ISHIKAWA YUJI (JP)
NISHIYAMA KIYOTO (JP)
KUZUHARA SHOJI (JP)
KINO YOICHIRO (JP)
SONODA KENGO (JP)
ISHIKAWA YUJI (JP)
NISHIYAMA KIYOTO (JP)
KUZUHARA SHOJI (JP)
KINO YOICHIRO (JP)
International Classes:
A61P31/12; C07K14/18; C12N7/08; A61K39/00; (IPC1-7): C12N7/04; C12N7/06; C12N7/02; A61K39/12; A61P31/12
Foreign References:
| JP2001514844A | 2001-09-18 | |||
| JPH05502581A | 1993-05-13 | |||
| JPH02223531A | 1990-09-05 | |||
| JPS5048118A | 1975-04-30 | |||
| JP2003523169A | 2003-08-05 |
Other References:
DEBNATH N.C. ET AL.: "In vitro homotypic and heterotypic interference by defective interfering particles of West ile virus", J. GEN. VIROL., vol. 72, no. 11, 1991, pages 2705 - 2711, XP002903497
BOROWSKI P. ET AL.: "Purification and characterization of West Nile virus nucleoside triphosphatase (NTPase)/helicase: evidence for dissociation of the NTPase and helicase activities of the enzyme", J. VIROL., vol. 75, no. 7, 2001, pages 3220 - 3229, XP002903498
MORITA K.: "Nishi Nile netsu no vaccine", MED. TECHNOL., vol. 32, no. 4, 15 April 2004 (2004-04-15), pages 347 - 348, XP002985309
SHLOMO LUSTIG ET AL.: "A live attenuated West Nile virus strain as a potential veterinary vaccine", VIRAL IMMUNOLOGY, vol. 13, no. 4, 2000, pages 401 - 410, XP008018324
BOROWSKI P. ET AL.: "Purification and characterization of West Nile virus nucleoside triphosphatase (NTPase)/helicase: evidence for dissociation of the NTPase and helicase activities of the enzyme", J. VIROL., vol. 75, no. 7, 2001, pages 3220 - 3229, XP002903498
MORITA K.: "Nishi Nile netsu no vaccine", MED. TECHNOL., vol. 32, no. 4, 15 April 2004 (2004-04-15), pages 347 - 348, XP002985309
SHLOMO LUSTIG ET AL.: "A live attenuated West Nile virus strain as a potential veterinary vaccine", VIRAL IMMUNOLOGY, vol. 13, no. 4, 2000, pages 401 - 410, XP008018324
Attorney, Agent or Firm:
Kawamiya, Osamu (IMP Building 3-7, Shiromi 1-chome, Chuo-k, Osaka-shi Osaka 01, JP)
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