Title:
WHOLE-CELL SYSTEM FOR CYTOCHROME P450 MONOOXYGENASES BIOCATALYSIS
Document Type and Number:
WIPO Patent Application WO/2014/202627
Kind Code:
A3
Abstract:
The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains.
Inventors:
GERBER ADRIAN (DE)
HANNEMANN FRANK (DE)
BLEIF SABRINA (CH)
KLESER MICHAEL (DE)
BERNHARDT RITA (DE)
HANNEMANN FRANK (DE)
BLEIF SABRINA (CH)
KLESER MICHAEL (DE)
BERNHARDT RITA (DE)
Application Number:
PCT/EP2014/062749
Publication Date:
March 12, 2015
Filing Date:
June 17, 2014
Export Citation:
Assignee:
SANOFI SA (FR)
International Classes:
C12P33/00; C12N9/02; C12N9/06; C12P7/62
Foreign References:
| US20100112634A1 | 2010-05-06 | |||
| US20030108982A1 | 2003-06-12 | |||
| EP0477961A2 | 1992-04-01 | |||
| EP2386634A1 | 2011-11-16 |
Other References:
SABRINA BLEIF ET AL: "A newwhole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-Î-boswellic acid (KBA) based on a recombinant cytochrome P450 system", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 93, no. 3, 22 July 2011 (2011-07-22), pages 1135 - 1146, XP035006085, ISSN: 1432-0614, DOI: 10.1007/S00253-011-3467-0
SINGH MAMTESH ET AL: "Bacillus subtilis as potential producer for polyhydroxyalkanoates.", MICROBIAL CELL FACTORIES 2009, vol. 8, 2009, pages 38, XP002711193, ISSN: 1475-2859
ROHINI D DESETTY ET AL: "Isolation and heterologous expression of PHA synthesising genes from Bacillus thuringiensis R1", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 24, no. 9, 2 February 2008 (2008-02-02), pages 1769 - 1774, XP019617091, ISSN: 1573-0972
SINGH MAMTESH ET AL: "Bacillus subtilis as potential producer for polyhydroxyalkanoates.", MICROBIAL CELL FACTORIES 2009, vol. 8, 2009, pages 38, XP002711193, ISSN: 1475-2859
ROHINI D DESETTY ET AL: "Isolation and heterologous expression of PHA synthesising genes from Bacillus thuringiensis R1", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 24, no. 9, 2 February 2008 (2008-02-02), pages 1769 - 1774, XP019617091, ISSN: 1573-0972
Attorney, Agent or Firm:
BLOT, Philippe et al. (2 place d'Estienne d'Orves, Paris, FR)
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