Title:
XYLITOL-PRODUCING STRAIN TO WHICH AN ARABINOSE METABOLIC PATHWAY IS INTRODUCED, AND METHOD FOR PRODUCING XYLITOL USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/046924
Kind Code:
A1
Abstract:
The present invention relates to a method for efficiently producing xylitol, which suppresses the production of the by-product arabitol by applying arabinose to cell metabolism in a complex medium of xylose and arabinose using a xylitol-producing strain to which an arabinose metabolic pathway is newly introduced. More particularly, the present invention relates to a method for producing xylitol suppressing the production of arabitol, which hinders purification and crystallization during the production of xylitol, by performing codon optimization so as to effectively express the L-arabinose isomerase (araA), L-ribulokinase (araB), and L-ribulose-5-phosphate 4-epimerase (araD) enzymes in Candida tropicalis strains; then inserting respective genes into a cassette including a promoter of glyceraldehyde-3-phosphate dehydrogenase and a selectable marker URA3; and transferring the latter to Candida sp. The xylitol-producing strain to which an arabinose metabolic pathway is newly introduced of the present invention can suppress the production of arabitol and can thus be useful in producing xylitol at a high yield.
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Inventors:
KIM JUNG HOE (KR)
YOON BYOUNG HOON (KR)
JEON WOO YOUNG (KR)
SHIM WOO YONG (KR)
YOON BYOUNG HOON (KR)
JEON WOO YOUNG (KR)
SHIM WOO YONG (KR)
Application Number:
PCT/KR2011/000346
Publication Date:
April 12, 2012
Filing Date:
January 18, 2011
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
KIM JUNG HOE (KR)
YOON BYOUNG HOON (KR)
JEON WOO YOUNG (KR)
SHIM WOO YONG (KR)
KIM JUNG HOE (KR)
YOON BYOUNG HOON (KR)
JEON WOO YOUNG (KR)
SHIM WOO YONG (KR)
International Classes:
C12N1/15; C12N15/52; C12P7/18
Foreign References:
| KR20090013617A | 2009-02-05 | |||
| US7745177B2 | 2010-06-29 | |||
| US20090004704A1 | 2009-01-01 | |||
| KR960030577A | 1996-08-17 | |||
| KR0169061B1 | 1999-01-15 | |||
| KR100259470B1 | 2000-06-15 | |||
| KR19950037516A | 1995-10-27 | |||
| KR19960013638A | 1996-04-30 | |||
| KR100730315B1 | 2007-06-19 |
Other References:
BEATE WIEDEMANN ET AL.: "Codon-Optimized Bacterial Genes Improve L-Arabinose Fermentation in Recombinant Saccharomyces cerevisiae.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 74, no. 7, 2008, pages 2043 - 2050, XP002494170
JESSICA BECKER ET AL.: "A Modified Saccharomyces cerevisiae Strain That Consumes I Arabinose and Produces Ethanol.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 69, no. 7, 2003, pages 4144 - 4150, XP009080581
HIDEO KAWAGUCHI ET AL.: "Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum.", APPL MICROBIOL BIOTECHNOL., vol. 77, no. 5, 2008, pages 1053 - 1062, XP019560773
BYOUNG SAM KO ET AL.: "Production of Xylitol from D-Xylose by a Xylitol Dehydrogenase Gene-Disrupted Mutant of Candida tropicalis.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 72, no. 6, 2006, pages 4207 - 4213, XP055058519
WINKELHAUSEN, E. ET AL., J. FERMENT. BIOENG., vol. 86, 1998, pages 1 - 14
GRANSTROM, T. B. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 74, 2007, pages 277 - 281
LAPLACE, J. M. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 36, 1991, pages 158 - 162
HAHN-HAGERDAL, B. ET AL., ENZYME MICROB. TECHNOL., vol. 16, 1994, pages 933 - 943
KIM, S. Y. ET AL., J. FERMENT. BIOENG., vol. 83, no. 3, 1997, pages 267 - 270
See also references of EP 2460871A4
JESSICA BECKER ET AL.: "A Modified Saccharomyces cerevisiae Strain That Consumes I Arabinose and Produces Ethanol.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 69, no. 7, 2003, pages 4144 - 4150, XP009080581
HIDEO KAWAGUCHI ET AL.: "Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum.", APPL MICROBIOL BIOTECHNOL., vol. 77, no. 5, 2008, pages 1053 - 1062, XP019560773
BYOUNG SAM KO ET AL.: "Production of Xylitol from D-Xylose by a Xylitol Dehydrogenase Gene-Disrupted Mutant of Candida tropicalis.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 72, no. 6, 2006, pages 4207 - 4213, XP055058519
WINKELHAUSEN, E. ET AL., J. FERMENT. BIOENG., vol. 86, 1998, pages 1 - 14
GRANSTROM, T. B. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 74, 2007, pages 277 - 281
LAPLACE, J. M. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 36, 1991, pages 158 - 162
HAHN-HAGERDAL, B. ET AL., ENZYME MICROB. TECHNOL., vol. 16, 1994, pages 933 - 943
KIM, S. Y. ET AL., J. FERMENT. BIOENG., vol. 83, no. 3, 1997, pages 267 - 270
See also references of EP 2460871A4
Attorney, Agent or Firm:
LEE, WON-HEE (KR)
이원희 (KR)
이원희 (KR)
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