Title:
YARROWIA LIPOLYTICA-DERIVED PEPTIDE-N-GLYCOSIDASE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/067466
Kind Code:
A2
Abstract:
The present invention relates to a novel peptide-N-glycosidase (PNGase Y) protein derived from Yarrowia lipolytica, to nucleic acids encoding said protein, to a recombinant vector comprising said nucleic acids, to a transformant transformed by said recombinant vector, to a method for preparing said protein by culturing said transformant, and to a method for preparing a glycoprotein in which an N-linked sugar chain is cut out using said protein or culture medium of said transformant comprising said protein. The peptide-N-glycosidase of the present invention may effectively cut out complex-type sugar chains, hybrid-type sugar chains, high-mannose-type sugar chains, sugar chains having mannose-6-phosphoric acids added thereto, sugar chains in which á(1,3)-fucose is added to a first N-acetylglucosamine, etc., and therefore exhibits wide and superior substrate specificity to sugar chains derived from animals, plants, or insects. The peptide-N-glycosidase of the present invention may cut out N-linked sugar chains not only when a glycoprotein is in a peptide state, but also when a protein is simply in a denatured state, and therefore may cut out N-linked sugar chains in a more convenient and quicker manner and enable the analysis of the structures of the sugar chains.
Inventors:
OH DOO BYOUNG (KR)
LEE KYUNG JIN (KR)
KWON OH SUK (KR)
KO KI SUNG (KR)
LEE KYUNG JIN (KR)
KWON OH SUK (KR)
KO KI SUNG (KR)
Application Number:
PCT/KR2011/008849
Publication Date:
May 24, 2012
Filing Date:
November 18, 2011
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
WONKWANG UNIV CT IND ACAD COOP (KR)
OH DOO BYOUNG (KR)
LEE KYUNG JIN (KR)
KWON OH SUK (KR)
KO KI SUNG (KR)
WONKWANG UNIV CT IND ACAD COOP (KR)
OH DOO BYOUNG (KR)
LEE KYUNG JIN (KR)
KWON OH SUK (KR)
KO KI SUNG (KR)
International Classes:
C12N9/24; C07K14/37; C12N1/19; C12N15/56; C12N15/81; C12P21/00
Foreign References:
| KR100915670B1 | 2009-09-04 | |||
| KR100378781B1 | 2003-04-07 | |||
| US20080124786A1 | 2008-05-29 |
Other References:
DATABASE NCBI 29 October 2008 Database accession no. XP 504069
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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Claims: