Title:
ZINC FLUORESCENT PROBE
Document Type and Number:
WIPO Patent Application WO/2007/013201
Kind Code:
A1
Abstract:
A zinc fluorescent probe that excels in water solubility, enabling observing with high sensitivity of any change of intracellular zinc ion level ratiometrically with the use of a single excited wavelength; a process for producing the same; and a method of measuring zinc ions with the use of the zinc fluorescent probe. As such a zinc fluorescent probe, there are provided compounds or salts thereof, the compounds represented by the general formula: (I) wherein Ar is an optionally substituted aryl; X is a group represented by -O- or -S-; n is 2 or 3; R1 and R2 may be identical with or different from each other and represent an optionally substituted alkyl, an optionally substituted aryl or an optionally substituted heteroaryl; Z is a single bond or a group of the formula: A is an optionally substituted benzene ring; and Y is a group represented by -O- or -S-.
Inventors:
TAKI MASAYASU (JP)
YAMAMOTO YUKIO (JP)
YAMAMOTO YUKIO (JP)
Application Number:
PCT/JP2006/304749
Publication Date:
February 01, 2007
Filing Date:
March 10, 2006
Export Citation:
Assignee:
UNIV KYOTO (JP)
TAKI MASAYASU (JP)
YAMAMOTO YUKIO (JP)
TAKI MASAYASU (JP)
YAMAMOTO YUKIO (JP)
International Classes:
C07D209/14; C07C205/37; C07D317/22; C07D319/06; G01N21/64; G01N21/78
Other References:
GRYNKIEWICZ G. ET AL.: "A New Generation of Ca2+ Indicators with Greatly Improved Fluorescence Properties", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, no. 6, 1985, pages 3440 - 3450, XP003002269
JEFFERSON J.R. ET AL.: "Characterization of indo-1 and quin-2 as spectroscopic probes for Zn2+-protein interactions", ANALYTICAL BIOCHEMISTRY, vol. 187, no. 2, 1990, pages 328 - 336, XP003002270
GEE K.R. ET AL.: "Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 5, 2002, pages 776 - 778, XP003002271
TAKI M. ET AL.: "Emission Ratiometric Imaging of Intracellular Zinc: Design of a Benzoxazole Fluorescent Sensor and Its Application in Two-Photon Microscopy", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 3, 2004, pages 712 - 713, XP003002272
JEFFERSON J.R. ET AL.: "Characterization of indo-1 and quin-2 as spectroscopic probes for Zn2+-protein interactions", ANALYTICAL BIOCHEMISTRY, vol. 187, no. 2, 1990, pages 328 - 336, XP003002270
GEE K.R. ET AL.: "Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 5, 2002, pages 776 - 778, XP003002271
TAKI M. ET AL.: "Emission Ratiometric Imaging of Intracellular Zinc: Design of a Benzoxazole Fluorescent Sensor and Its Application in Two-Photon Microscopy", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 3, 2004, pages 712 - 713, XP003002272
Attorney, Agent or Firm:
SAEGUSA, Eiji et al. (1-7-1 Doshomachi, Chuo-ku, Osaka-sh, Osaka 45, JP)
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