Title:
$g(a)-AGARASE AND PROCESS FOR PRODUCING THE SAME
Document Type and Number:
WIPO Patent Application WO/2000/050578
Kind Code:
A1
Abstract:
$g(a)-Agarase having the following physicochemical properties: (1) function: hydrolyzing an $g(a)-1,3 bond between 3,6-anhydro-L-galactose and D-galactose; (2) substrate specificity: acting on agarose and agarohexaose and higher agarooligosaccharides but not on agarotetraose; (3) optimum temperature: showing its enzymatic activity at 55 °C or below; and (4) heat stability: sustaining 20% or more of the activity after being treated at 48 °C for 30 seconds.
Inventors:
TOMONO JUN (JP)
NOMURA YOSHIKO (JP)
SAGAWA HIROAKI (JP)
SAKAI TAKESHI (JP)
KATO IKUNOSHIN (JP)
NOMURA YOSHIKO (JP)
SAGAWA HIROAKI (JP)
SAKAI TAKESHI (JP)
KATO IKUNOSHIN (JP)
Application Number:
PCT/JP2000/000966
Publication Date:
August 31, 2000
Filing Date:
February 21, 2000
Export Citation:
Assignee:
TAKARA SHUZO CO (JP)
TOMONO JUN (JP)
NOMURA YOSHIKO (JP)
SAGAWA HIROAKI (JP)
SAKAI TAKESHI (JP)
KATO IKUNOSHIN (JP)
TOMONO JUN (JP)
NOMURA YOSHIKO (JP)
SAGAWA HIROAKI (JP)
SAKAI TAKESHI (JP)
KATO IKUNOSHIN (JP)
International Classes:
C12N1/15; C12N1/21; C12N9/24; C12N15/31; C12N15/56; C12P19/04; C12P19/24; (IPC1-7): C12N9/40; C12N15/31; C12N15/56; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P19/14
Foreign References:
| JPH07322878A | 1995-12-12 | |||
| JPH07322886A | 1995-12-12 | |||
| US5834257A | 1998-11-10 |
Other References:
PHILIPPE POTIN ET AL.: "Purification and characterization of the alpha-agarase from Alteromonas agarlyticus(Cataldi) comb. nov., strai GJ1B", EUROPEAN JOURNAL OF THE BIOCHEMISTRY, vol. 214, no. 2, 1993, pages 599 - 607, XP002924340
Attorney, Agent or Firm:
Aoyama, Tamotsu (Shiromi 1-chome Chuo-ku, Osaka-shi Osaka, JP)
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