$g(b)-GALACTOSIDE $G(a)-2,6-SIALYLTRANSFERASE

Title:

$g(b)-GALACTOSIDE $G(a)-2,6-SIALYLTRANSFERASE

Document Type and Number:

WIPO Patent Application WO/1998/038289

Kind Code:

A1

Abstract:

$g(b)-Galactoside $g(a)-2,6-sialyltransferase produced by the genetic engineering technique. The enzyme may be deficient in at least a part of the membrane binding region possessed by a naturally occurring $g(b)-galactoside $g(a)-2,6-sialyltransferase and hence, when expressed in a host, is likely to be secreted outside cells, so that it can be easily prepared and purified. It is prepared by culturing a host cell transformed with an expression vector containing a DNA encoding the amino acid sequence of 16-498 in SEQ ID NO: 1 or DNA encoding a protein which has the same amino acid sequence as the above one, except that one or more amino acids have been deleted, substituted or added, and has a $g(b)-galactoside $g(a)-2,6-sialyltransferase activity under such conditions as will accelerate the expression of the $g(b)-galactoside $g(a)-2,6sialyltransferase, and recovering a $g(b)-galactoside $g(a)-2,6-sialyltransferase protein from the culture.

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Inventors:

YAMAMOTO TAKESHI (JP)
NAKASHIZUKA MOTOKO (JP)
TERADA ICHIRO (JP)

Application Number:

PCT/JP1998/000851

Publication Date:

September 03, 1998

Filing Date:

March 02, 1998

Export Citation:

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Assignee:

JAPAN TOBACCO INC (JP)
YAMAMOTO TAKESHI (JP)
NAKASHIZUKA MOTOKO (JP)
TERADA ICHIRO (JP)

International Classes:

C12N15/09; C07H21/04; C12N1/21; C12N9/10; C12N15/00; C12N15/31; C12R1/01; C12R1/19; (IPC1-7): C12N9/10; C12N15/31

Foreign References:

JPH08154673A

1996-06-18

Other References: