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Title:
ウイルス逆転写酵素活性の測定
Document Type and Number:
Japanese Patent JP2004500896
Kind Code:
A
Abstract:
In clinical settings as well as in a drug-discovery context, the impact of an agent that may affect reverse transcriptase (RT) can be measured and even quantified by bringing a sample, which may contain an RT, into contact with an RNA template, a primer complementary to the RNA template, and appropriate oligonucleotide-specific primers, under conditions such that they react, in the presence of RT, to form a cDNA product in inverse proportion to the effect of the agent. The amount of any resultant cDNA product then can be measured. The approach is readily implemented as a real-time, quantitative kinetic assay for RT activity.

Inventors:
ハリス,ロバート,ビー.
レイノルズ,トーマス,アール.
Application Number:
JP2002504689A
Publication Date:
January 15, 2004
Filing Date:
June 21, 2001
Export Citation:
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Assignee:
ハリス,ロバート,ビー.
International Classes:
C12Q1/48; C12Q1/68; C12Q1/70; (IPC1-7): C12Q1/48; C12Q1/68; C12Q1/70
Attorney, Agent or Firm:
平木 祐輔
藤田 節
石井 貞次



 
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