Title:
ウイルス逆転写酵素活性の測定
Document Type and Number:
Japanese Patent JP2004500896
Kind Code:
A
Abstract:
In clinical settings as well as in a drug-discovery context, the impact of an agent that may affect reverse transcriptase (RT) can be measured and even quantified by bringing a sample, which may contain an RT, into contact with an RNA template, a primer complementary to the RNA template, and appropriate oligonucleotide-specific primers, under conditions such that they react, in the presence of RT, to form a cDNA product in inverse proportion to the effect of the agent. The amount of any resultant cDNA product then can be measured. The approach is readily implemented as a real-time, quantitative kinetic assay for RT activity.
Inventors:
Harris, Robert, Bee.
Reynolds, Thomas, Earl.
Reynolds, Thomas, Earl.
Application Number:
JP2002504689A
Publication Date:
January 15, 2004
Filing Date:
June 21, 2001
Export Citation:
Assignee:
Harris, Robert, Bee.
International Classes:
C12Q1/48; C12Q1/68; C12Q1/70; (IPC1-7): C12Q1/48; C12Q1/68; C12Q1/70
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Ishii Teiji
Satoshi Fujita
Ishii Teiji