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Title:
固相のオリゴ糖タグ付け:固定化糖質の操作技術
Document Type and Number:
Japanese Patent JP2008530538
Kind Code:
A
Abstract:
The invention relates to methods of manipulating immobilised carbohydrates by derivatisation. Depending on the nature of the derivatisation, the carbohydrate may thereby be more easily detected and/or identified or handled. In particular, the invention relates to methods of preparing a reactive sugar comprising the steps of: i) providing a sample comprising a reducing sugar; ii) providing a solid support covalently attached to a linker comprising a capture group comprising an —NH2 group, wherein said linker optionally is attached to said solid support via a spacer; iii) reacting said reducing sugar with said —NH2 group, thereby obtaining an immobilised sugar; iv) reacting free —NH2 groups with a capping agent, wherein the capping agent comprises a reactive group capable of reacting with an —NH2 group; and v) reducing C═N bonds with a reducing agent, thereby obtaining an reactive sugar of the structure SugarCHn-NH— linked to a solid support via a linker and optionally a spacer, wherein n is 1 or 2.

Inventors:
ローセ、 アンデルス
ヨーゲンセン、 マレーネ リュボール
マルティンス、 リタ
ヒンドスガウル、 オレ
Application Number:
JP2007554420A
Publication Date:
August 07, 2008
Filing Date:
February 08, 2006
Export Citation:
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Assignee:
Merck Patent Gesellschaft mit beschraenkter Haftung
International Classes:
G01N33/53; C12Q1/34; C12Q1/48; C12Q1/68; G01N27/62; G01N33/483; G01N33/543; G01N33/547
Domestic Patent References:
JP2002350447A2002-12-04
JP2004333232A2004-11-25
Attorney, Agent or Firm:
宮崎 昭夫
石橋 政幸
緒方 雅昭