Title:
タプシガルジンにより誘導される細胞死の阻害剤
Document Type and Number:
Japanese Patent JP2010530736
Kind Code:
A
Abstract:
Methods for screening for inhibitors of endoplasmic reticulum (ER) stress are provided. These methods involve the addition of thapsigargin, which induces ER stress, and a test agent to mammalian cells in multi-well plates. Cell survival can be readily monitored by measuring intracellular ATP content using a bioluminescent reagent. Screening a commercially available library of 50,000 compounds led to the identification of 93 hit compounds that were subjected to secondary assays to confirm their ability to rescue cells from thapsigargin-induced cell death.
Inventors:
Reed, john, sea
Kim, ink
Kim, ink
Application Number:
JP2010509398A
Publication Date:
September 16, 2010
Filing Date:
May 23, 2008
Export Citation:
Assignee:
Burnham Institute for Medical Research
International Classes:
A61K31/404; C12Q1/02; A61K31/429; A61K31/4709; A61K31/495; A61K31/496; A61K31/517; A61K31/55; A61K31/5513; A61K45/00; A61P3/00; A61P3/06; A61P3/10; A61P9/00; A61P9/10; A61P25/00; A61P25/14; A61P25/16; A61P25/24; A61P25/28; A61P31/12; A61P43/00; C12N5/10; G01N33/15; G01N33/483; G01N33/50; C07D209/34; C07D209/86; C07D209/90; C07D239/91; C07D243/38; C07D295/18; C07D405/06; C07D409/12; C07D513/04
Domestic Patent References:
| JP2006254927A | 2006-09-28 |
Other References:
JPN6013019079; Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｖｏｌ．２８４， Ｎｏ．３, 2009, ｐｐ．１５９３-１６０３
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JPN6013019076; Ｎｅｕｒｏｃｈｅｍｉｓｔｒｙ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｖｏｌ．４８, 2006, ｐｐ．６９６-７０２
JPN6013019078; Ｃｅｌｌ Ｄｅａｔｈ ａｎｄ Ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ Ｖｏｌ．１０, 2003, ｐｐ．１１７８-１１８７
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Attorney, Agent or Firm:
Masutani Takeshi