Title:
ストレプトコッカスピオジェンスから誘導される組換えDNアーゼB
Document Type and Number:
Japanese Patent JP3742896
Kind Code:
B2
Abstract:
The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing efficient and rapid production of the DNase in E. coli without the necessity of growing large quantities of S. pyogenes. The enzyme can be produced with aleader peptide at its amino terminus. An improved method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.
Inventors:
Adams, Craig W
Bread, Patty Pee Wai
Beley, Sea Marina
Bread, Patty Pee Wai
Beley, Sea Marina
Application Number:
JP50801996A
Publication Date:
February 08, 2006
Filing Date:
August 18, 1994
Export Citation:
Assignee:
BECKMAN COULTER,INCORPORATED
International Classes:
C12N15/09; G01N33/569; A61K39/02; A61K39/09; A61K39/395; A61P31/04; C07H21/04; C07K14/315; C07K16/40; C12N1/21; C12N9/22; C12N15/55; C12P21/08; G01N33/573; A61K39/00; C12R1/19; C12R1/46
Domestic Patent References:
| JP6279498A | ||||
| JP8500741A | ||||
| JP63122957A |
Other References:
FEBS LETTERS, 1993年,vol.331, no.1-2, p.187-192
FEBS LETTERS, 1992年,vol.308, no.1, p.30-34
Laboratory and Clinical Medicine, 1980年,vol.95, no.2, p.258-265
FEBS LETTERS, 1992年,vol.308, no.1, p.30-34
Laboratory and Clinical Medicine, 1980年,vol.95, no.2, p.258-265
Attorney, Agent or Firm:
Nobuyuki Matsunaga