Title:
膜内外ｐＨ勾配ベシクルを調製する方法
Document Type and Number:
Japanese Patent JP6715265
Kind Code:
B2
Abstract:
A method for preparing transmembrane pH-gradient vesicles is provided. This method includes the following steps: a) preparing vesicles made from at least one matrix substance in an aqueous medium having an osmolarity of not more than 200 mOsm/l, wherein the matrix substance is chosen from the group consisting of amphiphilic lipids and amphiphilic block copolymers, b) transferring the vesicles into a basic or acidic buffer having an osmolarity being at least 200 mOsm/l higher than the osmolarity of the aqueous medium of step a) to apply an osmotic shock to the vesicles and to obtain buffer-filled vesicles and c) diluting a mixture of the aqueous medium and the basic or acidic buffer containing the buffer-filled vesicles by adding a neutralizing solution to obtain transmembrane pH-gradient vesicles suspended in a suspension buffer, wherein the suspension buffer differs from the basic or acidic buffer in pH value.
Inventors:
Leroy Jean-Christophe
Forster Vincent
Agostoni, Valentina
Forster Vincent
Agostoni, Valentina
Application Number:
JP2017557435A
Publication Date:
July 01, 2020
Filing Date:
May 03, 2016
Export Citation:
Assignee:
VERSANTIS AG
International Classes:
A61K9/127; A61K31/575; A61K31/685; A61K31/785; A61K47/02; A61K47/04; A61K47/10; A61K47/12; A61K47/18; A61K47/24; A61K47/26; A61K47/28; A61K47/60; A61L2/07; A61P39/02; B01J13/12; C08J5/00; C08K5/05; C08K5/521; C08L71/00
Domestic Patent References:
| JP2014506240A |
Other References:
ACSNANO, 2010, Vol.4, No.12, pp.7552-7558,Supplementary information pp.1-16
Attorney, Agent or Firm:
Shuji Sugimoto
Masashi Noda
Kenro Tsutsumi
Yuka Kobayashi
Kenichi Nakata
Daisuke Kaneko
Hiroki Taniguchi
Masashi Noda
Kenro Tsutsumi
Yuka Kobayashi
Kenichi Nakata
Daisuke Kaneko
Hiroki Taniguchi