Field of the Invention The present invention relates to compositions and methods to control fruit fly populations of the Tephritidae family.

Background of the Invention Fruit flies are a serious problem for the agriculture industry and cause extensive damage to, for example, citrus fruit. Extensive efforts have been made heretofore to exterminate these difficult and sometimes disease-bearing and/or fruit infecting insects.

A variety of methods have previously been utilized in efforts to control fruit flies.

Unfortunately, these methods have significant drawbacks which limit their usefulness.

For example, one common means for the control of fruit flies involves spraying malathion. Unfortunately, malathion, due to its toxicity, poses a serious threat to the environment and to human health.

Other methods of control have been attempted but without great success. For example, Enkerlin, W. et al. (Use of a Mixture of Boric Acid, Borax, Hydrolyzed Protein, and Water to Control Anastrepha Fruit Flies, Fruit Flies: Biology and Management, ed.

Aluja, P. and Liedo, P., Springer-Verlag, NY, Inc., pp 353-358 [1993]) suggest that borate compounds may be used as insecticides against fruit flies and that a mixture of boric acid, borax, hydrolyzed protein and water may be used to control Anastrepha fruit flies.

Lopez, F. D. et al. (J. Econ. Entomol. 61 (1): 316-317 [1968]) disclose the use of pelletized lures formulated with borax and either PIB. 7 (protein insect bait) or ENT-44, 014-X (enzyme hydrolyzed cottonseed protein) to trap and catch Mexican fruit flies.

Lopez, F. D. etal. (J. Econ. Entomol 60 (1): 137-140 [1967]) suggest that sodium borate inhibits decomposition of two protein hydrolysates attractive to the Mexican fruit fly.

Ken, A. J. et al. (Insect Pests Leaflets, Noll.-Fruit Flies, Gov't Printer, Dept. of Agriculture, N. S. W. Australia [1930]) disclose the use of lures containing borax to trap Mediterranean and Queensland fruit flies.

Newman, L. J. et al. (Fruit Fly (Ceratitis capitata); Baiting and Trapping Experiments, leaflet No. 244, Gov't Printer, Dept. Of Agriculture, Western Australia) disclose the use of arsenate of soda as a rapid killer of certain fruit flies and that trapping or luring methods utilizing arsenate of soda appear to be somewhat more effective than baiting methods.

Imidocloprid is an insecticidal compound known to have activity against sucking insects such as aphids, whiteflies, thrips, mealy bugs, leafhoppers, and scale insects. Use of imidocloprid to control fruit flies has not previously been disclosed or suggested.

There is a need in the art for improved toxicants that are effective against fruit flies of the family Tephritidae, that have attributes for direct and easy application, and that are not environmental pollutants or potential carcinogens.

Brief Summarv of the Invention The present invention provides novel methods and compositions for controlling fruit flies of the family Tephritidae.

In a preferred embodiment, the present invention pertains to the use of imidocloprid compounds to control fruit flies. While the preferred toxicant contemplated by the present invention is imidocloprid (1-[(6-chloro-3-pyridinyl) methy]-N-nitro-2- imidazolidinime), other suitable compounds including derivatives, analogs, and salts of imidocloprid can be used as described herein.

Also, in accordance with the present invention, the imidocloprid toxicants may be utilized alone or in combination with baits, insecticides, other toxicants, agars, liquefiers, sweeteners, carriers and the like.

It should be appreciated by those of skill in this art that the fruit flies of the Trephritidae family, as contemplated by the present invention, include the Caribbean fruit flies, the Mediterranean fruit flies, the Mexican fruit flies, the Oriental fruit flies and any

of the fruit flies which may be controlled by the methods and compositions of the present invention.

In accordance with the present invention, the methods and compositions are safe and effective and, therefore, can be used on any surface or at any location. In addition, the compositions of the present invention can be easily applied directly to areas of infestation and will remain active for extended periods of time. Therefore, the toxicants of the present invention may be used in residential preparations, commercial crop production, eradication programs and suppression programs for Tephritid fruit fly control.

The above features and advantages of the present invention will be better understood with reference to the detailed description and examples set out hereinafter.

It will also be understood that the specific methods and compositions as set forth herein are exemplary only and are not to be regarded as limitations of this invention.

Brief Description of the Drawings Figure 1 shows the chemical structure of imidocloprid.

Detailed Disclosure of the Invention The subject invention pertains to materials and methods useful for the safe and effective control of fruit flies. In a preferred embodiment, the subject invention pertains to the control of fruit flies which attack citrus. In accordance with the subject invention, fruit flies are controlled by the application of an imidocloprid compound.

The imidocloprid compound used according to the subject invention may be imidocloprid itself or an analog, derivative, or salt which retains the advantageous fruit fly controlling properties of imidocloprid. The chemical structure of imidocloprid is shown in Figure 1.

The fruit flies which can be controlled according to the subject invention include, but are not limited to, the Caribbean, Mediterranean, Mexican, and Oriental fruit flies.

The use of imidocloprid to control fruit flies as described herein is highly advantageous because imidocloprid is able to achieve a high level of control without toxicity to mammals, fish, or birds. The use of imidocloprid to control fruit flies has other important advantages including, for example, imidocloprid's low sensitivity to pH

changes. This property of imidocloprid is advantageous because it expands the options for formulating and applying imidocloprid. In this regard, imidocloprid has been found to be compatible with a variety of baits.

Advantageously, it has been found that the use of imidocloprid compounds as described herein to control fruit flies results in a high toxicity to mature male and female fruit flies.

The subject invention includes the use of imidocloprid either alone as the active ingredient, or in combination with other compounds which can improve the efficacy or ease of the treatment. In accordance with the present invention, the compositions for use in controlling fruit flies include mixtures such as a mixture of an imidocloprid toxicant in an effective amount and, for example, a protein hydrolysate bait or any synthetic bait to generate a bait or lure in the form of a patty, heavy cream, pellet, gel, foam, paste, liquid or spray. The bait or lure may be in the free form or, alternatively, in a form, such as granules or tablets, agglomerated with or without the aid of a binder. Moreover, the bait or lure can be fixed or impregnated on a support or absorbed therein, and this support may include for instance, agar, paper, cardboard, plastic such as polystyrene, polyvinyl chloride, polyvinyl acetate and cellulose acetate, glass, pumice, crushed marble, silica or silica minerals. Optionally, other toxicants, such as Malathion, Dibrom (b and NaledQ) can be used in conjunction with the imidocloprid application.

Furthermore, attractants, such as Male Lure 11 and methyl eugenol, sweeteners, carriers and/or liquefiers may be used together with the imidocloprid compound. A bait or lure may be placed in selected locations such that the fruit flies are likely to encounter and ingest the toxicant to assure the desired effect, but preferably out of the way of normal human or animal traffic.

One embodiment of the present invention pertains to the use of an imidocloprid compound in wide-area suppression and eradication programs. Currently, toxic pesticides, such as malathion, are formulated with a protein hydrolysate bait, such as Miller's Nu-LureO.

According to the current invention, imidocloprid can be substituted for malathion in the formulation. In a specific embodiment, the protein hydrolysates are corn-based. The protein hydrolysate may be used full-strength or diluted to about 10% with water. The final proteinaceous bait spray may be used over wide areas.

A second innovative method for use in accordance with this invention is to formulate the toxicant with an extender or gel, such as Min-U-GelOO, Thixcin Et, Myverolg and CAB-0-SILO. These are commercially available. In this case, gels can be sprayed in a solid stream to adhere to tree trunks, telephone poles, buildings and so forth. The gels are formulated with synthetic bait and/or natural proteinaceous baits.

This method of application reduces worker and public inconvenience of aerial spraying of large areas. For the homeowner, either the gel formulation or the liquid formulation may be applied to individual host trees for Tephritid fly control.

It, of course, should be understood by those versed in this art that the compositions of the present invention may be applied by any suitable means, such as by pressurized applications, hydraulic oil squirt cans and aerial sprays.

The imidocloprid may be applied at a rate of, for example, about 0.1 ppm to about 100 ppm. More preferably, the imidocloprid compound is applied at a rate of about 1 ppm to about 25 ppm. Most preferably, the imidocloprid compound is applied at a rate of about 5 ppm to about 20 ppm. The application rate can be readily optimized for a particular use by a person skilled in the art having the benefit of the instant disclosure.

In one embodiment of the subject invention, imidocloprid can be used in conjunction with a borax toxicant for the control of fruit flies. When imidocloprid is used in conjunction with a borate compound, the preferred borate compound is borax (sodium borate decahydrate-10 mol Na2B407 10H2O or sodium borate pentahydrate-5 mol Na2B407SH20). Other suitable borate compounds may be utilized in effective amounts as substitutes for borax or may be utilized in effective amounts in combination with borax or one another. Exemplary of borax-type compounds include anhydrous borax Na2B407, ammonium tetraborate (NH4) 2B407-4H20, ammonium pentaborate (NH4) 28 io0i a-8H20, potassium pentaborate K2BI0016-8H20, potassium tetraborate K2B407-4H20, sodium metaborate (8 mol) Na2B204-8H20, sodium metaborate (4 mol) Na2B204-4H20, disodium tetraborate decahydrate Na2B407-10H2O, disodium tetraborate <BR> <BR> <BR> <BR> <BR> pentahydrate Na2B407-5H20 and disodium octaborate tetrahydrate Na2B8Q3-4"20. thus, the term"borax toxicant (s)" is used herein broadly and includes collectively and/or individually such borax and any other suitable borax type compounds. An effective molarity for a borax toxicant is in the range of between about 0.02 M and about 0.12 M or higher.

The mixtures, formulations and combination treatments of the subject invention can be readily carried out by one skilled in the art having the benefit of the instant disclosure.

In brief, the present invention overcomes and alleviates certain of the above- mentioned drawbacks and shortcomings of the prior art and is directed to novel methods and compositions for killing or controlling fruit flies of the family Tephritidae.

It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application.