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Patent Searching and Data

Document Type and Number:
Japanese Patent JPH08510638
Kind Code:
The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

Jakobowitz, Edward Bee.
Siren, Joyel.
Levy, Mark Jay.
Goodman, Thomas C.
Becker, Martin Jay.
Ullman, Edwin F.
Caldwell, Robert M.
Bot, Richard Earl.
Burnet, Christopher Charles
Application Number:
Publication Date:
November 12, 1996
Filing Date:
April 07, 1994
Export Citation:
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Beringberge Akchengezel Shaft
Genenker International, Inc.
International Classes:
C12N9/04; C12N15/09; C12Q1/28; C12Q1/32; G01N33/535; G01N33/569; G01N33/542; G01N33/58; G01N33/78; G01N33/94; C12R1/19; (IPC1-7): C12N15/09; C12N9/04; C12Q1/32; G01N33/542
Attorney, Agent or Firm:
Takashi Ishida (3 others)