Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
APOPTOSIS-ASSOCIATED PROTEIN
Document Type and Number:
Japanese Patent JP2000083672
Kind Code:
A
Abstract:

To obtain a new protein consisting of a protein having a specific amino acid sequence, capable of binding with an ALG-2 protein associated with an apoptosis or a cell cycle adjustment and an inhibitor of which is useful for the diagnosis, treatment, etc., of the apoptosis, cancer, etc.

This apoptosis-associated antigen consists of amino acid sequences described as formulae I, II or III and can bind with a human origin ALG-2 protein associated with the apoptosis or a cell cycle adjustment, and used as a screening of inhibitors thereof, etc., since a compound inhibiting the binding of the protein with the ALG-2 protein is useful for an elucidation of the molecular mechanism of the apoptosis, cancer, etc., and the diagnosis and treatment of cancer, etc. The protein is obtained by adding a solubilized buffer cooled with ice to Jurkat cells, completely dissolving cells by a vigorous agitation, subjecting the cell solution to an ultra centrifuge, separating a supernatant, passing the same through a column fixed with an anti-ALG-2 antibody, and eluting the bound immuno-precipitated material with an eluting buffer.


Inventors:
SHIBATA MASAO
NOGUCHI JUN
Application Number:
JP1998000258165
Publication Date:
March 28, 2000
Filing Date:
September 11, 1998
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
IGAKU SEIBUTSUGAKU KENKYUSHO K
International Classes:
C12N15/09; A61K31/00; A61K45/00; A61K48/00; A61P35/00; A61P43/00; C07K14/47; C07K16/18; C12N1/21; C12N15/00; C12P21/02; C12R1/19; C12R1/91; (IPC1-7): C12N15/09; A61K31/00; A61K45/00; A61K48/00; A61P35/00; A61P43/00; C07K14/47; C07K16/18; C12N1/21; C12P21/02
Attorney, Agent or Firm:
Shimizu Hatsushi (1 person outside)