PURPOSE: To obtain a cell culture base which forms a cell aggregate not to cause dedifferentiation for a long period of time, is capable of eliminating floating cell aggregate, prevents necrosis of internal cells caused by aggregation of mutual floating cell aggregates, is capable of making cells cultured in vitro to develop functions of cells in vivo, maintaining the functions for a long period of time, is readily and inexpensively prepared and has high stability and to culture cells.

CONSTITUTION: In a method of cell culture, a cell culture base having the surface of the base composed of a vinyl-based copolymer containing 10-90mol% 2-hydroxyethyl methacrylate is used, cell aggregate is formed and cell aggregate is bonded to the surface of the base and maintained to give a cell culture base. Cells are cultured by using the cell culture base.