Title:
細胞培養システム
Document Type and Number:
Japanese Patent JP4845737
Kind Code:
B2
Abstract:
The present invention provides a novel apparatus to grow cells where the cultivation chamber (1) is partially filled with liquid cultivation medium and cells. Mixing and aeration is achieved by generating intermittently one single large gas bubble (6) at the bottom of the column bioreactor, the single large bubble width representing from 50 to 99% of the tank width, preferably from 60 to 99%, more preferably 98.5%. The culture medium flows out as a film between the large bubble and the inner wall of the bioreactor. This rising bubble allows mixing and aeration of the bulk. As the design of the invention is very simple, it is possible to manufacture it with flexible plastic material and use the apparatus as a disposable system. Moreover, such a mixing/aeration principle minimizes cell damages usually due to shear stress and small bubbles and allows easy and efficient scale-up from small scale to a larger one. Such a large-scale, efficient and disposable culture system can largely reduce production costs.
Inventors:
Cool Tor, Didier
Cubier, Arnaud, Robert, Jill
Enault, Nicholas
Cubier, Arnaud, Robert, Jill
Enault, Nicholas
Application Number:
JP2006540322A
Publication Date:
December 28, 2011
Filing Date:
November 18, 2004
Export Citation:
Assignee:
Nestec Society Anonymous
International Classes:
C12M1/00; B01F33/40; C12M1/04; C12M1/06; C12N1/00; C12N5/00; C12P1/00
Domestic Patent References:
| JPH0649143B2 | 1994-06-29 | |||
| JPS61212283A | 1986-09-20 |
Attorney, Agent or Firm:
Yoshiki Hasegawa
Tomoya Kurokawa
Yoshinori Shimizu
Keiko Iizuka
Tomoya Kurokawa
Yoshinori Shimizu
Keiko Iizuka