Title:
SYNTHESIS OF CHROMOSOME DNA AND PRODUCTION OF CHROMOSOME
Document Type and Number:
Japanese Patent JP3220845
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To readily obtain a train of chromosome DNA having a centromere region, a telomere region and a replication-initiating region by polymerizing deoxyribonucleotide in a reaction system in the absence of a template and a primer, etc., and in the presence of a protein.
SOLUTION: In a reaction system not containing a template and/or a primer, a manifesting vector containing a DNA coding a protein composed of a heat- resistant deoxyribonucleotide polymerase, etc., of a bacterium belonging to genera Thermococcus, Thermus or Pyrococcus is introduced into a cell and the protein is produced in the cell, then the deoxyribonucleotide is polymerized in the presence of the protein at 60-90°C to readily obtain the objective chromosome DNA composed of a train of double stranded linear DNA having a centromere region, a telomere region and a replication-initiating region as necessary elements in the chromosome.
Inventors:
Norio Ogata
Takanori Miura
Takanori Miura
Application Number:
JP15664796A
Publication Date:
October 22, 2001
Filing Date:
June 18, 1996
Export Citation:
Assignee:
Daiko Pharmaceutical Co., Ltd.
International Classes:
C12N15/09; C12N9/12; C12R1/01; (IPC1-7): C12N15/09
Domestic Patent References:
| JP9285289A | ||||
| JP5317058A |
Other References:
【文献】国際公開96/7669(WO,A1)
Attorney, Agent or Firm:
Hitoko Tsuda (1 person outside)