Title:
遺伝子操作によるデキストランスクラーゼDSR-Sの新規変異体の構築
Document Type and Number:
Japanese Patent JP2009525745
Kind Code:
A
Abstract:
The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the alpha-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthesis of alpha-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.
Inventors:
Mont Saint Pierre
Remo-Simeon, Magari
Potski-Veronese, Gabriel
Muri, Clair
Remo-Simeon, Magari
Potski-Veronese, Gabriel
Muri, Clair
Application Number:
JP2008553855A
Publication Date:
July 16, 2009
Filing Date:
February 08, 2007
Export Citation:
Assignee:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Institute National de la Lecherche Agronomic
Institute National de la Lecherche Agronomic
International Classes:
C12N15/09; A23L1/30; C07H1/00; C07H3/04; C07H3/06; C07K7/06; C07K14/195; C07K19/00; C08B37/02; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P19/08; C12P21/02
Domestic Patent References:
| JP2005185290A | 2005-07-14 | |||
| JP2003533219A | 2003-11-11 | |||
| JPH07274991A | 1995-10-24 | |||
| JPH0198601A | 1989-04-17 | |||
| JP2005034135A | 2005-02-10 | |||
| JPH02289520A | 1990-11-29 | |||
| JPH07274991A | 1995-10-24 | |||
| JPS63240757A | 1988-10-06 | |||
| JP2005034135A | 2005-02-10 |
Foreign References:
| WO1989012386A1 | 1989-12-28 | |||
| WO1989012386A1 | 1989-12-28 |
Other References:
JPN6012019611; Ａｐｐｌ．Ｅｎｖｉｒｏｎ．Ｍｉｃｒｏｂｉｏｌ．，１９９８ Ｍａｙ，６４（５），ｐ．１６４４-９
JPN6012019611; Ａｐｐｌ．Ｅｎｖｉｒｏｎ．Ｍｉｃｒｏｂｉｏｌ．，１９９８ Ｍａｙ，６４（５），ｐ．１６４４-９
JPN6012019612; Ａｎｔｏｎｉｅ Ｖａｎ Ｌｅｅｕｗｅｎｈｏｅｋ，２００５ Ｆｅｂ，８７（２），ｐ．１３１-４１
JPN6012019611; Ａｐｐｌ．Ｅｎｖｉｒｏｎ．Ｍｉｃｒｏｂｉｏｌ．，１９９８ Ｍａｙ，６４（５），ｐ．１６４４-９
JPN6012019612; Ａｎｔｏｎｉｅ Ｖａｎ Ｌｅｅｕｗｅｎｈｏｅｋ，２００５ Ｆｅｂ，８７（２），ｐ．１３１-４１
Attorney, Agent or Firm:
Hajime Tsukuni
Koshiro Tsukuda
Fusayuki Saito
Koshiro Tsukuda
Fusayuki Saito