PURPOSE: To correctly determine a sample to be tested in high sensitivity by affecting combination of creatininamide hydrolase and creatinamidino hydrolase, etc., to sample to be tested containing creatinine.
CONSTITUTION: (a) 50-500unit/ml creatininamide hydrolase is mixed with (b) 40-300unit/ml creatinamidino hydrolase, (c) 20-200unit/ml urease, (d) 5-15mM α-ketoglutalic acid, (e) 1-10unit/ml glutamic acid dehydrogenase and (f) 0.15-0.30mM nicotinamide adenine dinucleotide phosphate of reduction type so as to be pH7.5-8.5 to obtain a reagent composition for determination of creatinine (A). Next, the component A is added to a sample to be tested containing creatinine and reacted at about 37°C for about 10min, then creatinine is determined by reduced amount of the f-component. On the other hand, a reagent composition (B) subtracted the a-component from the component A is affected to a sample to be tested containing creatine in a similar manner as the above-mentioned to determine creatine.
KIMURA NORIYUKI
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