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Title:
DEVICE AND METHOD FOR GENE DIAGNOSIS
Document Type and Number:
Japanese Patent JP3781688
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To provide a device and a method for gene diagnosis capable of easily and accurately detecting a gene aberration of one base or more in a short time and reducing the seize, weight, and costs of the device for automating the diagnosis at a low running cost.
SOLUTION: This gene diagnosis device is provided with a high temperature part splitting a double helix of a DNA sample and a separation part adjusted at a temperature lower than that of the high temperature part for separating the split single stranded DNA sample between a normal DNA, an abnormal DNA, and a noise DNA. In a buffer solution inside a sealed flow passage, the DNA sample and a separating DNA conjugate, in which a base sequence and a polymer compound bondable with the single stranded DNA sample via hydrogen bonds are bonded for separating the DNA sample between the normal DNA, the abnormal DNA, and the noise DNA according to a difference of bonding strength, are charged. When a constant voltage is applied, the DNA inside the sealed flow passage is electrophoresed, and a passage amount of the normal DNA and that of the abnormal DNA are measured respectively by means of a detection part.


Inventors:
Hideaki Hashimoto
Kazuyoshi Mori
Takeshi Nishida
Mizuo Maeda
Yoshiki Katayama
Application Number:
JP2002042942A
Publication Date:
May 31, 2006
Filing Date:
February 20, 2002
Export Citation:
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Assignee:
Matsushita Electric Industrial Co., Ltd
Mizuo Maeda
Yoshiki Katayama
International Classes:
G01N27/447; G01N33/483; C12M1/00; C12M1/34; C12M1/38; C12M1/42; C12N15/09; C12Q1/68; G01N33/50; (IPC1-7): G01N27/447; C12M1/00; C12M1/34; C12M1/38; C12M1/42; C12N15/09; C12Q1/68; G01N33/483; G01N33/50
Domestic Patent References:
JP2001252098A
JP6138090A
JP5332992A
Other References:
有澤卓己,キャピラリー電気泳動による遺伝子変異診断法の開発,九州大学大学院工学研究科 材料物性工学専攻 前田研究室 卒業論文,日本,2000年 3月
穴田貴久,DNAコンジュゲートを用いる遺伝子一塩基変位診断システムの開発,日本分析化学会第49年会講演要旨集,日本,2000年 9月,p.180
Attorney, Agent or Firm:
Fumio Iwahashi
Daisuke Nagano
Hiroki Naito