PURPOSE: An aqueous extract of leaves of SOKUHAKU (Thujae orientalis cacumen) is extracted with an organ ic solvent, then subjected to gel-filtration column chromatography and chromatography to effect siolation and purification to obtain simply quercitrin which is used as a hemostatic.
CONSTITUTION: Leaves of SOKUHAKU is extracted with hot water and the extract is fractionated with an ethyl acetate-water solvent mix system and the ethyl acetate phase is separated. The resultant lipid-soluble substances are separated by means of gel-filtration column chromatography using methanol as a eluent to collect the hemostatic fractions. Further, the fractions are purified by subjecting them to silica-gel chromatography and gel-filtration column chromatography to obtain the objective quercitrin.
ISHIDA KINJI
ITOU YASUO
KATOU HIDEO