PURPOSE: To determine SP1 in a number of sample liquids quickly and accurately by a radio immunoassay by arranging an anti-human SP1 (pregnancy specific protein) antibody immobilizing vessel, a radioactive substance labeled human SP1 labeled human SP1 and a buffer liquid.
CONSTITUTION: Labeled human SP1 is dissolved into a buffer liquid to prepare a standard calibration curve. Radioactive substance labeled human SP1 is preferably 125I or 135I labeled one and the labeling of human SP1 is done by the chloroamine T method or the like. A vessel is preferably a test tube-shaped on male of a synthetic resin. Anti-human SP1 antibody is obtained by emulsifying human SP1 with Freund's Adjuvant and sensitizing the emulsion to an animal such as rabbit and guinea pig. The anti-human SP1 antibody is diluted in the buffer liquid and after it is allowed to stand in the vessel, the dilusion liquid is removed. Then, the product is washed and dried to obtained anti-human SP1 antibody immobilization vessel. The radioactive substance-labeled SP1 and a buffer liquid are added to the vessel and after allowed to stand, it is washed to make an antibody immobilization vessel in which about 40W60% of the labeled SP1 is bonded. The buffer liquid herein used is, for example, a 0.1M phosphate- buffered physiological salt solution with pH7.
FUKUSHIMA TSUNEKAZU
FUNAKOSHI SATORU
SUYAMA TADAKAZU
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