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Title:
METHOD FOR HYDROLYZING PROTEIN WITH HYDROLASE AND DEVICE THEREFOR
Document Type and Number:
Japanese Patent JP2788837
Kind Code:
B2
Abstract:

PURPOSE: To obtain a sufficiently defined peptide profile with good reproducibility by mixing a protein hydrolase with a protein substrate, performing a first hydrolysis process in an agitation tank and also performing a second hydrolysis process within a tube provided with a stationary mixing element.
CONSTITUTION: This method comprises: supplying measured amounts of a protein substrate and a protein hydrolase to an agitation tank 1 from a vessel 7 and a vessel 13 respectively to perform a first hydrolysis process there while agitating them with an agitator 3; and then, transferring the first hydrolysis product to a tube 25 provided with a stationary mixing element 27 to perform a second hydrolysis process there. At this time, the residence time of the total amount of the substrate can accurately and simply be adjusted.


Inventors:
JOANNU BENSHU
ANTOWAANU MARUGO
NIKURO MEISUTERU
ARUBEERU RANKAN
ROBEERU DEYUSUTAN UDO
ARUFURETSUDO UPEI
Application Number:
JP8344993A
Publication Date:
August 20, 1998
Filing Date:
April 09, 1993
Export Citation:
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Assignee:
PURODEYUI NETSUSURU SA SOC
International Classes:
A23J3/08; A23J3/14; A23J3/16; A23J3/20; A23J3/34; C07K14/47; C12M1/06; A23J3/04; C12P21/06; (IPC1-7): A23J3/34; A23J3/04; A23J3/08; A23J3/14; A23J3/16; A23J3/20; C12P21/06
Domestic Patent References:
JP1167200B
JPS59162873A
JPH0198494A
JPS63258599A
JPS6098993A
JPS60192599A
JP64005484A
JPS5898084A
JPS5176443A
Attorney, Agent or Firm:
Akira Asamura (3 outside)