イメージングのためのビーズの調製のための方法

Japanese Patent JP6997717

B2

The invention relates to a process for the preparation of beads comprising a biocompatible hydrophobic polymer, a perfluorocarbon and optionally a metal compound, comprising the steps of: a) adding the perfluorocarbon and optionally the metal compound to a solution of the biocompatible hydrophobic polymer in a polar solvent to provide a first liquid mixture, b) adding the first liquid mixture to an aqueous solution of a biocompatible surfactant under sonication to obtain a second liquid mixture, c) maintaining the sonication of the second liquid mixture while cooling, d) evaporating the polar solvent from the second liquid mixture to obtain a suspension of beads comprising the biocompatible hydrophobic polymer, the perfluorocarbon and optionally the metal compound, e) separating the beads from the suspension and preparing a water suspension of the beads and f) freeze-drying the water suspension to obtain the beads, wherein the addition of the first liquid mixture to the biocompatible surfactant in step b) is performed within a period of at most 10 seconds, wherein the sonication in step b) and the sonication in step c) are performed by a probe at an amplitude of at least 240 µm for 1-10 minutes and wherein the weight ratio of the biocompatible surfactant to the biocompatible hydrophobic polymer is at least 3:1.

Srinivas Mangala
Koshikina Olga
Figdor Caruru Gustav
De Vries Ingrid Yolanda Monique

JP2018547899A

February 04, 2022

March 13, 2017

Click for automatic bibliography generation  

Stichting Radbaud Universitere Medish Stichting

A61K49/12; A61B8/13; A61B8/14; A61K9/16; A61K47/02; A61K47/06; A61K47/34; A61K49/06; A61K49/10; A61K49/22; G01N21/64; G01N21/65; G01N24/00; G01N24/08

WO2014041150A1
CN101574530A

Biomaterials, 2010, Vol.31, pp.7070-7077

Patent Service Corporation Patent Office Sykes

Previous Patent: Stem cells expressing mesenchymal and neuronal markers, their compositions, and methods of their pre...

Next Patent: New co-crystal