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Title:
MUTATED APOPROTEIN OF LOW CALCIUM-SENSITIVE PHOTOPROTEIN
Document Type and Number:
Japanese Patent JP2012217357
Kind Code:
A
Abstract:

To provide a calcium-binding photoprotein having slow attenuation of emission.

The mutated apoprotein contains an amino acid sequence in which amino acids of 23rd to 34th in an amino acid sequence of sequence number 2 are substituted with amino acids represented by formula I: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa34, has a function of binding a peroxide of coelenterazine or a peroxide of coelenterazine analog and forming a photoprotein to produce luminescence by a calcium ion action, and has a half-life period of emission caused by binding of the photoprotein with calcium ion of two seconds or more.


Inventors:
INOUE SATOSHI
MIURA YUIKO
Application Number:
JP2011000083968
Publication Date:
November 12, 2012
Filing Date:
April 05, 2011
Export Citation:
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Assignee:
JNC CORP
International Classes:
C12N15/09; C07K1/02; C07K14/435; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; C12Q1/68; G01N33/53; G01N33/532
Attorney, Agent or Firm:
小林 浩
片山 英二
星川 亮
鈴木 康仁



 
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