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Title:
N-ACETYLGLUCOSAMINYL-CELLOOLIGOSACCHARIDE DERIVATIVE AND METHOD FOR PRODUCING THE SAME
Document Type and Number:
Japanese Patent JP2003212891
Kind Code:
A
Abstract:

To provide a non-natural type new cellooligosaccharide derivative, and to provide a method for producing the same.

This method for producing the new cellooligosaccharide derivative, an N-acetylglucosaminly-cellooligosaccharide derivative, comprises the following process: using a cellooligosaccharide or a derivative thereof as a saccharide receptor and an oxazoline derivative of N-acetyllactosamine as a saccharide donor, a chitinase is subjected to them under alkaline conditions to obtain a non-natural type N-acetyllactosaminyl-cellooligosaccharide derivative where N-acetyllactosamine is transferred via a β-1,4-linkage to the non-reduced terminal of the cellooligosaccharide or the derivative thereof; subsequently, β-D galactosidase is subjected to the non-natural type N-acetyllactosaminyl- cellooligosaccharide derivative to obtain the objective N-acetyl glucosaminyl- cellooligosaccharide derivative where N-acetylglucosamine is transferred via a β-1,4-linkage to the non-reduced terminal of the cellooligosaccharide or the derivative thereof; wherein it is preferable that the chitinase (preferably, Bacillus-derived one) be subjected to both the saccharide receptor and donor under the condition of pH 8-10.


Inventors:
SHODA SHINICHIRO
MISAWA YOSHITOMO
KURIYAMA MASAHISA
FUJITA MASAYA
WATANABE TSUYOSHI
Application Number:
JP2002000011613
Publication Date:
July 30, 2003
Filing Date:
January 21, 2002
Export Citation:
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Assignee:
YAIZU SUISANKAGAKU IND CO LTD
International Classes:
C12P19/26; C07H15/14; (IPC1-7): C07H15/14; C12P19/26
Attorney, Agent or Firm:
松井 茂