To design and develop an optical arrangement in a beam path of a light source suitable for fluorescence excitation, preferably in a beam path of a confocal laser scanning microscope such that injection of excitation light of various excitation wavelengths is possible without having to switch or make special adjustment to the optical elements used when switching the wavelength of the excitation light.
The optical arrangement comprises at least one spectrally selective element (4) for injecting excitation light (3) of at least one light source (2) in the microscope and for separating and extracting the excitation light (3) scattered and reflected on an object (10) or for extracting the excitation light from light (13) coming from the object (10) via a detection beam path (12). The optical arrangement in the beam path of the light source suitable for fluorescence excitation, preferably in the beam path of the confocal laser scanning microscope is characterized in that excitation light (3, 9) of different wavelengths can be separated and extracted by the spectrally selective element (4) so that configuration can be changed with the simplest structure. Alternatively, the optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be separated and extracted.
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BRADL JOACHIM
ULRICH HEINRICH
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