Title:
RNAポリメラーゼから誘導されたポリペプチド及びそれらの使用
Document Type and Number:
Japanese Patent JP2004535163
Kind Code:
A
Abstract:
Mutant RNA polymerases of phagic origin in which the peptide chain is modified by substitution, deletion or addition of at least one amino acid, the modification having the effect of reducing the sensitivity of the RNA polymerases to the initial transcription sequence of the DNA sequence coding for the RNA, for a method for production of the RNA, or proteins coded by the RNA, from given nucleotide sequences, comprising a sequence of DNA coding for the RNA, the transcription of which is placed under the control of a promoter recognised by wild-type RNA polymerases and the mutant RNA polymerases as above. The method has a higher yield of RNA than the yield obtained when using the wild-type RNA polymerases in the presence of the same non-consensual ITS as that found in the sequence of DNA coding for the RNA.
Inventors:
Guillerez, Jean
Lopez, Pascal
Mark, Dreyfus
Lopez, Pascal
Mark, Dreyfus
Application Number:
JP2002574354A
Publication Date:
November 25, 2004
Filing Date:
March 19, 2002
Export Citation:
Assignee:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12; C12N15/54; C12P21/00; C12P21/02; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12; C12P21/02
Attorney, Agent or Firm:
Hajime Tsukuni
Fumio Shinoda
Fumio Shinoda