Title:
PREPARATION OF WEAKLY ACTIVE OR INACTIVE ALKALINE PHOSPHATASE MUTANT, AND ITS EXPRESSION IN YEAST
Document Type and Number:
Japanese Patent JP3850806
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for preparing a weakly active or inactive alkaline phosphatase mutant and a method for expressing the mutant in an yeast.
SOLUTION: The invention relates to a method for the recombinant production or expression of eukaryotic alkaline phosphatase (AP) mutants in yeast cells wherein the specifically introduced mutations result in a reduction in specific activity of AP by at least a factor 1:100. The invention also relates to a method for inserting the corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it relates to the corresponding vectors and host strains.
Inventors:
Liner mueller
Johann-Peter Tarhofer
Frank gaipel
Werner Heerke
Thomas Kirschbaum
Johann-Peter Tarhofer
Frank gaipel
Werner Heerke
Thomas Kirschbaum
Application Number:
JP2003080470A
Publication Date:
November 29, 2006
Filing Date:
March 24, 2003
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12N15/09; C12N1/19; C12N9/16; C12N15/55; C12R1/84; (IPC1-7): C12N15/09; C12N1/19; C12N9/16; //(C12N1/19; C12R1:84); (C12N9/16; C12R1:84)
Foreign References:
| EP1176205A1 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Ishii Teiji
Satoshi Fujita
Ishii Teiji