Title:
高スループット小分子薬物発見のための培養ヒト肥満細胞および好塩基球の産生
Document Type and Number:
Japanese Patent JP4388372
Kind Code:
B2
Abstract:
Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.
Inventors:
Rossi, Alexander Bee.
Application Number:
JP2003525600A
Publication Date:
December 24, 2009
Filing Date:
August 29, 2002
Export Citation:
Assignee:
Rigel Pharmaceuticals, Inc.
International Classes:
C12N15/09; C12N5/02; C12N5/0787; C12Q1/02
Foreign References:
| US4559310 |
Other References:
炎症,1999年 3月10日,Vol. 19, No. 2,pp. 93-98
Clinical & Experimental Allergy,1998年,Vol. 28, No. 10,pp. 1228-1236
Clinical & Experimental Allergy,2000年,Vol. 30, No. 8,pp. 1053-1055
blood,1997年,Vol. 90, No. 2,pp. 620-629
Annual Review 血液 1997,1997年 1月30日,pp. 18-24
Clinical & Experimental Allergy,1998年,Vol. 28, No. 10,pp. 1228-1236
Clinical & Experimental Allergy,2000年,Vol. 30, No. 8,pp. 1053-1055
blood,1997年,Vol. 90, No. 2,pp. 620-629
Annual Review 血液 1997,1997年 1月30日,pp. 18-24
Attorney, Agent or Firm:
Naoto Uchida
Koji Okuhara
Koji Okuhara