To provide a protein originated from Pieris rapae crucivora (common white butterfly), having specific properties such as cytotoxic activity on cultured human gastric cancer cell TMK-1, etc., exhibiting strong anticancer activity at a low concentration, having low toxicity to normal cell and expected to be useful as an anticancer agent.
This protein originated from Pieris rapae crucivora has the following properties. (A) Molecular weight: 6.7×104 to 4.4×105, (B) temperature stability: stable at 40°C and inactivated at 60°C, (C) pH stability: stable at pH3-9, (D) solubility: soluble in pure water and PBS and insoluble in chloroform and ether, (E) enzymatic stability: stable to Pronae, Proteinase-K, Nuclease P1, (Spleen) phosphodiesterase and BAP, (F) adsorbability: adsorbable to DEAE and hydroxyapatite and free from absorptivity to phosphocellulose and Affigel Blue, (G) precipitation property: forming a precipitate with an aqueous solution of ammonium sulfate of ≥40% concentration and (H) cytotoxicity: exhibiting cytotoxic activity on cultured human gastric cancer cell TMK-1.
SUGIMURA TAKASHI
WAKABAYASHI KEIJI
KOIWAI KAZUMICHI
KOYAMA TSUNETARO