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Title:
RECOMBINANT OBESITY(OB) PROTEIN
Document Type and Number:
Japanese Patent JP3244627
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To produce a new recombinant obesity(ob) protein useful for the treatment, prevention, control, etc., of obesity and its relating diseases by expressing a fused protein containing an obesity(ob) protein and an E.coli signal peptide in E.coli cell and isolating the ob protein.
SOLUTION: The objective protein is a new human recombinant obesity(ob) protein having an amino acid sequence expressed by the formula, a homogeneous structure and a biological activity or its fragment having the biological activity of the protein. The protein is useful for the treatment, prevention, control, etc., of obesity and its relating diseases. The ob protein can be produced by constructing an expression vector having a promoter sequence and a DNA sequence coding for a fused protein containing a protein expressed by the formula and a signal paptide of an exosporium protein A of E.coli, inserting the expression vector into a host E.coli cell to transform the E.coli cell, expressing the fused protein in the host cell and treating the host cell with a cold osmotic pressure shock buffering solution to isolate the ob protein.


Inventors:
Campfield, Arthur
Devos, Rene
Guisez, Yves
Application Number:
JP11273996A
Publication Date:
January 07, 2002
Filing Date:
May 07, 1996
Export Citation:
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Assignee:
F HOFFMANN LA ROCHE AG
International Classes:
A61K38/00; A61K38/17; A61K47/48; A61P3/04; A61P3/06; A61P3/08; A61P3/10; A61P9/10; C07H21/04; C07K1/18; C07K1/20; C12N15/09; C07K1/22; C07K14/195; C07K14/41; C07K14/47; C07K14/575; C07K19/00; C12N1/21; C12N15/10; C12N15/11; C12N15/113; C12N15/12; C12N15/62; C12N15/63; C12N15/64; C12N15/70; C12P21/02; C12R1/19; (IPC1-7): C12N15/09; A61K38/00; A61P3/04; C07K14/47; C07K19/00; C12N1/21; C12P21/02
Other References:
Nature(1994)Vol.372,p.425−432
Attorney, Agent or Firm:
津国 肇