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Title:
SRM ASSAY FOR INDICATING CANCER THERAPY
Document Type and Number:
Japanese Patent JP2019049557
Kind Code:
A
Abstract:
To provide specific peptides, and derived ionization characteristics of the peptides, which are particularly advantageous for directly quantifying ALK, Ros, Ron, Ret, TS, and/or FGFR1 proteins in biological samples fixed in formalin by the SRM/MRM) methods.SOLUTION: A biological sample is selected from tissues and cells treated with formaldehyde-containing agents/fixatives, including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from the biological sample using the Liquid Tissue (TM) reagents and protocol, and the proteins are quantified by quantifying one or more peptides using the SRM/MRM mass spectrometry methods.SELECTED DRAWING: None

Inventors:
DAVID B KRIZMAN
TODD HEMBROUGH
SHEENO THYPARAMBIL
LIAO WEI-LI
Application Number:
JP2018182742A
Publication Date:
March 28, 2019
Filing Date:
September 27, 2018
Export Citation:
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Assignee:
EXPRESSION PATHOLOGY INC
International Classes:
G01N27/62; A61K45/00; A61P35/00
Attorney, Agent or Firm:
Hiroyuki Nagai
Nakamura Yukitaka
Yasukazu Sato
Satoru Asakura
Asano Mari
Sorimachi Hiroshi
Nobumi Ikeda