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Title:
THERMOSTABLE LUCIFERASE AND METHOD OF PRODUCTION
Document Type and Number:
Japanese Patent JP2010104377
Kind Code:
A
Abstract:

To provide mutant luciferase enzymes with greatly enhanced thermostability in comparison with natural luciferases or mutant luciferases.

A modified luciferase conjugate is prepared by using a beetle luciferase gene, applying it to various means for inducing random mutation, particularly to gene synthesis using a polymerase easily happening to be in errors, preparing a modified luciferase gene library, expressing the same with E. coli, and combining selected mutations. The method includes recursive mutagenesis in which a novel library is prepared from modified luciferases through random mutation induction and the process is repeatedly selected.


Inventors:
WOOD KEITH V
HALL MARY P
Application Number:
JP2010000006999
Publication Date:
May 13, 2010
Filing Date:
January 15, 2010
Export Citation:
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Assignee:
PROMEGA CORP
International Classes:
C12N15/09; C07K19/00; C12N1/21; C12N9/02; C12N11/00; C12N15/10; C12N15/53; C12P21/02; C12Q1/02; C12Q1/66; C12Q1/68
Domestic Patent References:
JP2001516585A2001-10-02
JPH05244942A1993-09-24
JP2001516585A2001-10-02
JPH05244942A1993-09-24
Foreign References:
WO1995025798A11995-09-28
WO1996022376A11996-07-25
WO1995025798A11995-09-28
WO1996022376A11996-07-25
Other References:
JPN6012033319; Biochemical Journal,1996,Vol.319,part2,p.343-350
JPN7012002441; Biochemistry,1996,Vol.61,No.1,p.115-119
JPN6012033320; Biochim. Biophys. Acta,1996, Vol.1339, No.1, pp.39-52
JPN6012033319; Biochemical Journal,1996,Vol.319,part2,p.343-350
JPN7012002441; Biochemistry,1996,Vol.61,No.1,p.115-119
JPN6012033320; Biochim. Biophys. Acta,1996, Vol.1339, No.1, pp.39-52
Attorney, Agent or Firm:
川口 義雄
渡邉 千尋
大崎 勝真
坪倉 道明