Title:
【発明の名称】アミノ酸分析のためのアミノ酸蛍光誘導体混合物とアミノ酸分析のためのアミノ酸蛍光誘導体混合物の製造方法とアミノ酸蛍光誘導体混合物をアミノ酸分析に使用する方法
Document Type and Number:
Japanese Patent JP2518637
Kind Code:
B2
Abstract:
A new mixture of fluorescent amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which all primary amino acids are present in one kind of derivatization whereas all secondary amino acids are present in another kind of derivatization. …The process for recovering the mixture comprises the steps of derivatization of the primary amino acids of an amino acid mixture with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.
Inventors:
JEIMUSU AREKISANDAA APUFUERU JUNIA
RAINAA SHUSUTAA
RAINAA SHUSUTAA
Application Number:
JP3898887A
Publication Date:
July 24, 1996
Filing Date:
February 20, 1987
Export Citation:
Assignee:
HEWLETT PACKARD CO
International Classes:
G01N30/84; B01D15/08; C07C67/00; C07C239/00; C07C271/10; C07C271/22; C07D209/44; G01N30/74; G01N30/88; G01N33/68; (IPC1-7): C07C271/22; C07D209/44; G01N30/74; G01N33/68
Attorney, Agent or Firm:
Hideo Ueno