Title:
タンパク質の高発現システム
Document Type and Number:
Japanese Patent JP4526638
Kind Code:
B2
Abstract:
According to the protein expression system in which a variety of desired useful proteins are highly produced by fusing a coding region of useful protein gene to the downstream of a promoter region of a tyrosinase-encoding gene (melO) of Aspergillus oryzae by a usual method of DNA manipulation, transferring a plasmid containing the resulting novel fusion gene into Aspergillus oryzae and incubating the thus-obtained transformant, various proteins can efficiently be produced at a high purity and in a high yield.
Inventors:
Yoji Hata
Hiroki Ishida
Hiromoto Hisada
Eiji Ichikawa
Akitsugu Kawato
Abe Yasuhisa
Koji Suginami
Satoshi Imayasu
Hiroki Ishida
Hiromoto Hisada
Eiji Ichikawa
Akitsugu Kawato
Abe Yasuhisa
Koji Suginami
Satoshi Imayasu
Application Number:
JP2000036754A
Publication Date:
August 18, 2010
Filing Date:
February 15, 2000
Export Citation:
Assignee:
Laurel wreath
International Classes:
C07K14/38; C12N15/09; C12N1/14; C12N1/15; C12N9/34; C12N15/80; C12P21/02; C12R1/69
Domestic Patent References:
| JP10084968A |
Other References:
Biochim Biophys Acta.,1995年,Vol.1261,pp.151-154
Appl Microbiol Biotechnol.,1998年,Vol.50,pp.459-467
Biosci Biotechnol Biochem.,1996年,Vol.60,pp.383-389
Biochem J.,1969年,Vol.114,10P
FEMS Microbiol Lett.,1990年,Vol.60,pp.195-199
Appl Microbiol Biotechnol.,1998年,Vol.50,pp.459-467
Biosci Biotechnol Biochem.,1996年,Vol.60,pp.383-389
Biochem J.,1969年,Vol.114,10P
FEMS Microbiol Lett.,1990年,Vol.60,pp.195-199
Attorney, Agent or Firm:
Matsumoto Kuki
Toda parent man
Toda parent man