Title:
【発明の名称】十字花科植物から有効成分の分離方法
Document Type and Number:
Japanese Patent JP2534043
Kind Code:
B2
Abstract:
PURPOSE:To separate and recover two active components consisting of a hot component and a peroxidase from a cruciferous plant at the same time, by using supercritical carbon dioxide or liquefied carbon dioxide as an extraction solvent. CONSTITUTION:A cruciferous plant (e.g. raw horseradish) is thrown into a cooling tank 1, frozen e.g. with liquid nitrogen and pulverized with a pulverizer 2 in frozen state. The obtained frozen powder is filled in an extraction tank 3 and heated to cause enzymatic reaction and produce a hot component (allyl isothiocyanate). At the same time, supercritical carbon dioxide is introduced into the extraction tank 3 at >=30 deg.C and >=75 atm or liquid carbon dioxide is introduced at 20 deg.C under 200 atom to extract the produced hot component to the solvent. The solvent is transferred to a separation tank 4 and separated into a liquid phase and a gaseous phase by reducing the pressure. The gaseous phase is released from the system to selectively separate and recover the hot component. A peroxidase can be extracted and separated from the residue left in the extraction tank 3 by the conventional method.
Inventors:
KOBAYASHI TAKESHI
TANIGUCHI MASAYUKI
TANIGUCHI MASAYUKI
Application Number:
JP23375186A
Publication Date:
September 11, 1996
Filing Date:
October 01, 1986
Export Citation:
Assignee:
KINJIRUSHI WASABI KK
KOBAYASHI TAKESHI
KOBAYASHI TAKESHI
International Classes:
C12N9/08; C07C67/00; C07C325/00; C07C331/18; C07C331/22; (IPC1-7): C12N9/08; C07C331/18
Domestic Patent References:
| JPS61129003A | ||||
| JPS61227787A | ||||
| JPS5928473A |
Attorney, Agent or Firm:
Someya Hitoshi